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试验采用温湿度二因素回归最优设计,研究贵烟4号变黄阶段烘烤环境与烤后烟叶石油醚提取物含量及香吃味的关系,结果表明:在温度32℃~42℃、相对湿度75%~95%范围内,随着变黄温湿度的逐渐升高,烤后烟叶石油醚提取物含量及香吃味评吸得分均表现出先逐渐增加而后又下降的趋势;在控制变黄温度约37℃~38℃,相对湿度84%~90%即干湿差约1.5℃~2℃烤后烟叶石油醚提取物含量及香吃味评吸得分较高;分析结果还表明,烤后烟叶的石油醚提取物含量与香吃味评吸得分表现出较好的相关性。 相似文献
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木材干燥导水系数和换水系数的研究 总被引:3,自引:0,他引:3
木材(板、方材)的导水系数和换水系数是反映木材干燥或存放过程中水分迁移的重要物性参数。然而,我国对木材导水系数和换水系数的研究和测定工作十分有限。木材干燥有关的理论计算中,常用原苏联的数据。由于这些数据本身可能存在的误差及用于我国树种的可靠程度难以估计,故使理论结果的实际运用受到限制。本文采用等厚试件系数分离法研究和测定了木材干燥过程动态导水系数和换水系数。 相似文献
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河南省食用菌科技推广存在的问题及对策 总被引:1,自引:0,他引:1
综合分析了河南省食有菌科技推广中存在的生产经营缺乏宏观引导,科技推广存在自发性和盲目性,体制不协调和食用菌生产本身的不可控因素较多等问题,提出了加强宏观引导,微观调控,建立健全食用菌行业管理,食用菌龙头企业建设,开发建设规模生产基地等具体措施,为食用菌的产业化大发展提供了参考依据。 相似文献
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将 30头试验鹿随机分为 3组 ,Ⅰ组为对照组 ,Ⅱ、Ⅲ组为试验组 ,分别在日粮中添加 5 0mg/kg安定和 10 0IU/kg鹿安宁。观测保定—锯茸应激状态下 ,梅花鹿血液白细胞的数量和组成、血浆总糖皮质激素、皮质醇浓度及淋巴细胞糖皮质激素受体含量的变化。结果表明 ,安定和鹿安宁均可显著提高应激鹿血液白细胞总数 (P <0 0 1) ,鹿安宁可使之趋于正常 (P >0 0 5 ) ;安定和鹿安宁均可显著提高应激鹿血液中淋巴细胞百分率 (P <0 0 1) ,降低N/L值 (P <0 0 1)。安定和鹿安宁可显著降低应激鹿血浆总糖皮质激素、皮质醇浓度 ,提高应激鹿血液淋巴细胞糖皮质激素受体含量 (P <0 0 1)。综合比较 ,鹿安宁的作用效果较安定好 相似文献
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A. Schots J. De Boer A. Schouten J. Roosien J. F. Zil Verentant H. Pomp L. Bouwman-Smits H. Overmars F. J. Gommers B. Visser W. J. Stiekema J. Bakker 《European journal of plant pathology / European Foundation for Plant Pathology》1992,98(2):183-191
Engineering resistance against various diseases and pests is hampered by the lack of suitable genes. To overcome this problem we started a research program aimed at obtaining resistance by transfecting plants with genes encoding monoclonal antibodies against pathogen specific proteins. The idea is that monoclonal antibodies will inhibit the biological activity of molecules that are essential for the pathogenesis. Potato cyst nematodes are chosen as a model and it is thought that monoclonal antibodies are able to block the function of the saliva proteins of this parasite. These proteins are, among others, responsible for the induction of multinucleate transfer cells upon which the nematode feeds. It is well documented that the ability of antibodies to bind molecules is sufficient to inactivate the function of an antigen and in view of the potential of animals to synthesize antibodies to almost any molecular structure, this strategy should be feasible for a wide range of diseases and pests.Antibodies have several desirable features with regard to protein engineering. The antibody (IgG) is a Y-shaped molecule, in which the domains forming the tips of the arms bind to antigen and those forming the stem are responsible for triggering effector functions (Fc fragments) that eliminate the antigen from the animal. Domains carrying the antigen-binding loops (Fv and Fab fragments) can be used separately from the Fc fragments without loss of affinity. The antigen-binding domains can also be endowed with new properties by fusing them to toxins or enzymes. Antibody engineering is also facilitated by the Polymerase Chain Reaction (PCR). A systematic comparison of the nucleotide sequence of more than 100 antibodies revealed that not only the 3′-ends, but also the 5′-ends of the antibody genes are relatively conserved. We were able to design a small set of primers with restriction sites for forced cloning, which allowed the amplification of genes encoding antibodies specific for the saliva proteins ofGlobodera rostochiensis. Complete heavy and light chain genes as well as single chain Fv fragments (scFv), in which the variable parts of the light (VL) and heavy chain (VH) are linked by a peptide, will be transferred to potato plants. A major challenge will be to establish a correct expression of the antibody genes with regard to three dimensional folding, assembly and intracellular location. 相似文献
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Tsutomu MATSUMOTO Yuichiro NARA Hiromitsu FURUYA Harumi TAKAHASHI Kiichi TAIRAKO Hideki YAMAMOTO 《Journal of General Plant Pathology》2002,68(4):382-384
L11A-Fukushima (L11A-F) derived from attenuated isolate LuA of Tomato mosaic virus (ToMV) has the highest ability to cross protect against virulent ToMV among LuA and its derivatives and is stably inherited.
Growth, yield, fruit quality and symptom attenuation of inoculated tomato plants did not differ significantly between L11A-F and L11A. The infectivity of progeny viruses in tomato infected with LuA-F was less than 4% of that with virulent ToMV. From these
results, L11A-F appears to possess the properties necessary for practical use. To manage L11A-F strictly, a PCR-based assay to detect trace contamination of virulent ToMV in L11A-F preparations was established.
Received 10 June 2002/ Accepted in revised form 30 October 2002 相似文献
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Shohei MATSUURA Shigeru HOSHINO Hideaki HAYASHI Tetsuyuki KOHGUCHI Kyoji HAGIWARA Toshihiro OMURA 《Journal of General Plant Pathology》2002,68(1):99-102
DAS-ELISA proved to be reliable enough to detect a latent infection by Tomato spotted wilt virus (TSWV) in asymptomatic stock plants of chrysanthemum. A high density of Frankliniella occidentalis, the predominant vector, in the presence of latently infected stock plants resulted in a high incidence of disease in the chrysanthemum
production field. The incidence of disease was low when the vector thrips were not abundant in spite of the presence of latently
infected stock plants. These results suggest that an infestation of the vector thrips causes severe secondary spread of TSWV
originating from latently infected stock plants in chrysanthemum production fields.
Received 27 July 2001/ Accepted in revised form 27 November 2001 相似文献