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Abolfazl Daneshvar Mulualem Tigabu Asaddollah Karimidoost Per Christer Odén 《林业研究》2017,28(2):231-239
The germination of freshly collected Juniperus polycarpos (K. Koch) seeds is very low and attributed to the large proportion of nonviable seeds in the seed lots. Thus, the aim of this study was to improve seed lot quality by removing nonviable seeds using two flotation techniques: incubation-drying-separation (IDS) and modified specific gravity (MSG) separation. We examined different IDS conditions (the specific incubation time, subsequent drying time and sorting media) for effectively sorting out nonviable seeds; and tested the feasibility of modified SG separation, which involved soaking seeds in water for a certain period before sorting in different concentrations of sucrose solution. Viable seeds were expected to absorb and metabolically bind more water during soaking than dead seeds could, and hence be sorted effectively depending on the viscosity of the sucrose solution. The viability of the seeds that floated or sank was determined in a topographical tetrazolium chloride (TTC) test. For the IDS trial, 7 days incubation followed by 9 h of drying and sedimentation in pure water or 200 g·L?1 sucrose solution identified, respectively, 75 and 82 % of the seeds as viable (sunken) seeds. For the MSG trail, 77 % viable seeds were recovered in the sunken fraction when seeds were soaked for 48 h then immediately sorted in 600 g·L?1 sucrose solution. In both cases, the loss of viable seeds in the discarded floating fraction was only 4 %. The results demonstrate that both IDS and MSG separation techniques substantially improved seed lot viability, but MSG separation is simple and needs no modern seed handling facility, and its efficacy relies on seed mass (due to the initial soaking) and the specific density and viscosity of the flotation medium. 相似文献
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Abolfazl Daneshvar Mulualem Tigabu Mostafa Farhadi Per Christer Odn Asaddollah Karimidoost 《林业研究》2014,25(4):827-834
Dwarf mistletoes are parasitic flowering plants that infect conifers, resulting in substantial loss of growth and mortality. Recently, forest managers in Iran are contemplating whether infection of Juniperus polycarpos C. Koch forests by dwarf mistletoe, Arceuthobium oxycedri (DC.) M. Bieb, influences tree vigor and contributes to insuffieient natural regeneration. The present study aimed at assessing the severity of infection and its impact on growth and reproductive output of./., polycar- pos. Infected and uninfected trees (n =20 each) were selected for assess- ment of diameter, height, crown area, and crown volume as well as quantity and quality of cones and seeds. The severity of infection of trees was determined by Hawksworth's 6-class dwarf mistletoe rating (DMR) system. The DMR system revealed that 40% of the infected sample trees were lightly infected (DMR =1-2) and 60% were moderately infected (DMR =3--4). Growth characteristics did not differ significantly (p 〉 0.05) between infected and uninfected trees. However, moderate infec- tion affected the reproductive output of./. polycarpos by significantly (p 〈0.05) reducing the mean number of cones per unit area of the crown, increasing the number of damaged seeds, and reducing seed size and seedgermination capacity. We conclude that reproductive output of J.. poly- carpos is more sensitive than growth characters to moderate infection by juniper dwarf mistletoe, and this might partly account for poor natural regeneration. 相似文献
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