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1.
Hybridization in the Urochloa humidicola breeding programme allowed to explore the genetic variability of the apomictic sources and, thus, to obtain new sexual and apomictic parents with compatible ploidy and/or superior progenies. However, given the high variability arising from crosses, there is a need to adopt efficient selection strategies among and within progenies. The aim of this study was to compare the selection methods among U. humidicola progenies with (t-BLUP) and without (BLUP) the weighting based on prediction error and subsequent selection within progenies by individual simulated (BLUPIS) or optimal individual BLUP. We evaluated seventy-one U. humidicola full-sib progenies from biparental crosses between nine sexual and ten apomictic parents. The experiment was set up in an incomplete block design with 64 blocks of 15 plots each, consisting of individual plants. Seven consecutive cuts were made and the traits total dry-matter, leaf dry-matter and regrowth scores were measured. Genetic variance was expressive for all traits. The selection procedure among progenies of U. humidicula based on t-BLUP proved to be more advantageous regarding the use of BLUP not weighted by the prediction error, resulting in higher selection gains, taking as reference the optimal procedure of the individual BLUP. Furthermore, the use of BLUPIS allowed an optimized selection of hybrids, allowing the breeder to explore with variable selection intensity the genetic variability within the selected full-sib progenies based on relative genetic merit.  相似文献   
2.

The goal of sustainable coffee production requires multiple functions from agroforestry systems. Many are difficult to quantify and data are lacking, hampering the choice of shade tree species and agronomic management. Process-based modelling may help quantify ecosystem services and disservices. We introduce and apply coffee agroforestry model CAF2021 (https://doi.org/10.5281/zenodo.5862195). The model allows for complex systems with up to three shade tree species. It simulates coffee yield, timber and fruit production by shade trees, soil loss in erosion, C-sequestration, N-fixation, -emission and -leaching. To calibrate the model, we used multivariate data from 32 different treatments applied in two long-term coffee agroforestry experiments in Costa Rica and Nicaragua. Without any further calibration, the model was then applied to agroforestry systems on 89 farms in Costa Rica and 79 in Guatemala where yields had been reported previously in farmer interviews. Despite wide variation in environmental and agronomic conditions, the model explained 36% of yield variation in Costa Rica but only 15% in Guatemala. Model analysis quantified trade-offs between yield and other ecosystem services as a function of fertilisation and shading.

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3.
Changes in coffee economics are leading producers to reduce agrochemical use and increase the use of shade. Research is needed on how to balance the competition from shade trees with the provision of ecological services to the coffee. In 2000, long-term coffee experiments were established in Costa Rica and Nicaragua to compare coffee agroecosystem performance under full sun, legume and non-legume shade types, and intensive and moderate conventional and organic inputs. Coffee yield from intensive organic production was not significantly different from intensive conventional in Nicaragua, but in Costa Rica it was lower during three of the six harvests. Full sun coffee production over 6 years was greater than shaded coffee in Costa Rica (61.8 vs. 44.7 t ha?1, P = 0.0002). In Nicaragua, full sun coffee production over 5 years (32.1 t ha?1) was equal to coffee with shade that included Tabebuia rosea (Bertol.) DC., (27?C30 t ha?1) and both were more productive (P = 0.03) than coffee shaded with Inga laurina (Sw.) Willd. (21.6 t ha?1). Moderate input organic production was significantly lower than other managements under all shade types, except in the presence of Erythrina poepiggina (Walp.) O.F. Cook. Inga and Erythrina had greater basal area and nutrient recycling from prunings than other shade species. Intensive organic production increased soil pH and P, and had higher K compared to moderate conventional. Although legume shade trees potentially provide ecological services to associated coffee, this depends on management of the competition from those same trees.  相似文献   
4.
As most mountains in tropical and subtropical zones, the Western Sierra Madre suffers active present erosion, which may create some constraints to the social and economic development in the area.The objectives of this study of soil degradation in the Western Sierra Madre, are to determine the respective roles of gully and sheet erosion. This research is based on field observations, field measurements of runoff and, soil losses at the plot, as well as the watershed scales as an analysis of an exhaustive census of the few gullies located in an experimental area.Measured soil losses in the Western Sierra Madre are high although there are few gullies. Most of the sediment yield seems to originate in widespread degraded areas where stoniness is the main evidence of a previous stage of erosion. Previously overgrazing and deforestation were determined as the factors of the appearance of new soil surface characteristics which explain the high runoff and sediment productions. The soil compacted by cattle trampling reduces infiltration. The decrease of the vegetation cover triggers a rise in the splash effect and thus, a soil sealing.These processes induce an increase in runoff and soil losses. The main erosion type has been described as sheet erosion: it is characterised by the removal of fine soil particles and the remains of gravels, pebbles and blocks, which constitute a pavement on the soil. Gullies generally appear on the bottom of wide valleys and depressions, where soils are thick. It is shown that sheet erosion is two orders of magnitude higher than gully erosion at the hillslope scale.Due to the spatial distribution of land use and the geological context such as the heavily degraded areas close to the main rivers, the reduction of runoff and soil loss rates within the extension of a considered area, commonly observed in hydrology, only applies up to the elementary catchments scale (1 to 50 km2). Above this area, runoff coefficient and soil loss rates increase.  相似文献   
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6.
The potato leafroll virus (PLRV) P0 protein (P0PL) is a suppressor of RNA silencing. In this study, we showed that P0 protein from an Argentinian isolate of PLRV (P0PL-Ar) has an additional activity not described for other PLRV or P0 proteins from poleroviruses. Besides reporting that P0PL-Ar displays both local and systemic silencing suppressor activity, we demonstrated, for the first time, that P0PL-Ar impedes accumulation of dsRNA-derived siRNAs. We also showed that P0PL-Ar interacts with Solanum tuberosum SKP1 orthologue (StSKP1) and triggers destabilization of ARGONAUTE 1 (AGO1) and that these actions are mediated by the F-box-like domain. A mutant in the GW/WG motif within the P0PL-Ar F-box-like motif lost the suppression activity, the interaction with StSKP1 and abolished AGO1 decay. Interestingly, a mutant in the L76/P77 residues within the P0PL-Ar F-box-like motif, which lost the suppression activity and the interaction with StSKP1, retained the capacity to enable AGO1 decay. Thus, unlike other P0 proteins of previously characterized poleroviruses, P0PL-Ar seems to have a dual activity, according to the findings of this study. This protein would act at both an upstream and a downstream step of the RNA silencing pathway: upstream of Dicer-like enzyme (DCL)-mediated primary siRNA production and downstream at the RNA-induced silencing complex (RISC) complex level. Our results contribute to the understanding of the different ways PLRV P0 proteins function as silencing suppressors.  相似文献   
7.
Agricultural studies in seasonally flooded areas in the tropics have been limited. Orinoco alluvial soils are more fertile than adjacent non-flooded soils, and have been considered as potential areas for further agricultural development. Traditional agroforestry practices offer some possibilities to overcome the most limiting factors of floodplain cultivation. Indigenous knowledge of these traditional agroforestry practices was assessed and some indicators of the system's nitrogen and biomass dynamics were monitored. Nitrogen inputs by sediment and plant residues were 1668 and 47 kg ha–1, respectively. Traditional selection of sediment by tree-and-grass barriers and litter management strategies increase nutrient-use efficiency in floodplain agroecosystems.
Resumen Estudios agrícolas en areas tropicales sujetas a inundación estacional han sido limitados. Los suelos aluviales o vegas del Orinoco son más fértiles que suelos cercanos no sujetos a inundación y por lo tanto han sido considerados como areas potenciales para desarrollos agrícolas. Prácticas tradicionales de agroforestería constituyen una alternativa en el control de importantes factores limitantes en la agricultura de vegas. El conocimiento técnico autóctono asociado con prácticas agroforestales tradicionales de vegas fue evaluado y algunos indicadores de la dinámica de nitrógeno y biomas adel sistema fueron monitoreados. La contribución de nitrógeno por sedimentos y hojarasca fue de 1668 and 47 kg ha–1 respectivamente. La selección del tipo de sedimento a depositarse en la parcela agrícola, por medio de barreras de vegetación, y el manejo adecuado de resíduos orgánicos constituyen estrategias tradicionales que aumentan la eficiencia de uso de nutrientes en sistemas agrícolas de vegas.
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9.
New esterase substrates were synthesized using cis-, trans- and cis-trans-permethrinic acid chloride and then used to measure pyrethroid-cleaving enzymes in insects. The new substrates, namely cis-, trans- and cis-trans-7-coumaryl permethrates (7-CP), show a structure very similar to permethrin insecticide and yield fluorescent products on hydrolysis. These substrates were hydrolyzed by a commercial porcine preparation that provided esterase-specific activity, and were stable at different pH values (5.2-7.8). Studies made with house fly, Musca domestica (L.), homogenates showed that these compounds are appropriate for determining pyrethroid hydrolysis activity on individual insects. The measured activity of house fly esterase was 870 relative fluorescence units (RFU) min(-1) with cis-7-CP as substrate, 1117 RFU min(-1) with trans-7-CP as substrate and 1423 RFU min(-1) with cis-trans-7-CP as substrate. The fluorescent substrates for pyrethroid-cleaving esterases described in this paper have advantages over methods already given in the literature. They are substrates with structures very similar to pyrethroids, the cleavage of which can be followed by an increase in fluorescence emission at 440 nm; it takes only about 5 min to measure the reaction, and moreover the high sensitivity of the fluorescence technique allows the quantification of esterase activity on individual insects.  相似文献   
10.
This study presents a comparative morphological and morphometrical examination of the interosseous muscle (IOM), digital flexor tendons and associated structures of all four species of South American Camelids (SACs): Lama (Lama glama), alpaca (Vicugna pacos), guanaco (Lama guanicoe) and vicuna (Vicugna vicugna). Our findings show that the lamoid interosseous muscle and digital flexor tendons are very similar between species and differences, which have been reported previously, are possibly due to diverging examiner interpretation. Lumbricalis muscles were mostly found as single muscles in this study. The definition of the terminal and dorsal branches of the interosseous muscle is critically revised. The analysis of morphometric data indicates that the size of the individual has a substantial influence on the cross-sectional area (CSA) of the examined structures. Metacarpal/metatarsal bone width is a main predictor of CSA, while the factor species only shows significance in specific sample points. For lama and alpaca, no differences in CSA in relation to species as factor were found. The IOM shows the largest CSAs of the three examined structures in all species, which implies an important load-bearing function. Considerable individual variation remains unexplained by the mentioned factors. This lowers the prospect of CSA reaching diagnostic importance in SAC fetlock pathology.  相似文献   
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