Construction of Brucella abortus A19 VirB Promoter Deletion Strain and Study on Its Biological Characteristics     

DENG Xiaoyu  HE Jinke  YANG Qin  YI Jihai  WANG Yueli  XI Jing  WANG Zhen  CHEN Chuangfu 《中国畜牧兽医》2020,47(3):864-872

  相似文献   

Preparation and properties of solid polymer electrolyte based on imidazolium-based ionic liquids for structural capacitors     

Bong-Sang Cho  Jun Choi  Ki-Young Kim 《Fibers and Polymers》2017,18(8):1452-1458

We prepared solid polymer electrolytes (SPEs) composed of poly(ethylene glycol) monomethyl ether acrylate (1A9OMe) and 1-ethyl-3-methylimidazolium trifluoromethanesulfonate ([EMIm][OTF]) and 1-ethyl-3-methylimidazolium bis(trifluoromethane sulfonyl) imide ([EMIm][TFSI]) as the ionic liquid. The SPEs formed by appropriately adding ionic liquids in the 1A9OMe prior to thermal cure. The ratio of 1A9OMe and ionic liquid was 1:9, 3:7, and 5:5, respectively. The characterization of solid polymer electrolytes were investigated using Fourier transform infrared spectroscopy in the attenuated total reflectance mode (FTIR-ATR), Electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), and glavanostatic charge-discharge test. The highest ionic conductivity of SPEs was found to be 4.90×10^?4 S/cm in a 1A9OMe/[EMIm][OTF] of 3:7. As IL contents were increased, the specific capacitance of supercapacitor was increased. The specific capacitance of supercapacitor for ionic liquid with large ion size was lower than that for ionic liquid with smaller ion size.  相似文献   

Quantitative trait loci analysis for root traits in synthetic hexaploid wheat under drought stress conditions          下载免费PDF全文

LIU Rui-xuan  WU Fang-kun  YI Xin  LIN Yu  WANG Zhi-qiang  LIU Shi-hang  DENG Mei  MA Jian  WEI Yu-ming  ZHENG You-liang  LIU Ya-xi 《农业科学学报》2020,19(8):1947-1960

Synthetic hexaploid wheat(SHW), possesses numerous genes for drought that can help breeding for drought-tolerant wheat varieties. We evaluated 10 root traits at seedling stage in 111 F9 recombinant inbred lines derived from a F2 population of a SHW line(SHW-L1) and a common wheat line, under normal(NC) and polyethylene glycol-simulated drought stress conditions(DC). We mapped quantitative trait loci(QTLs) for root traits using an enriched high-density genetic map containing 120 370 single nucleotide polymorphisms(SNPs), 733 diversity arrays technology markers(DArT) and 119 simple sequence repeats(SSRs). With four replicates per treatment, we identified 19 QTLs for root traits under NC and DC, and 12 of them could be consistently detected with three or four replicates. Two novel QTLs for root fresh weight and root diameter under NC explained 9 and 15.7% of the phenotypic variation respectively, and six novel QTLs for root fresh weight, the ratio of root water loss, total root surface area, number of root tips, and number of root forks under DC explained 8.5–14% of the phenotypic variation. Here seven of eight novel QTLs could be consistently detected with more than three replicates. Results provide essential information for fine-mapping QTLs related to drought tolerance that will facilitate breeding drought-tolerant wheat cultivars.  相似文献   

Prevalence of the enteroaggregative Escherichia coli heat-stable enterotoxin 1 gene and its relationship with fimbrial and enterotoxin genes in E. coli isolated from diarrheic piglets.     

C Choi  D Kwon  C Chae 《Journal of veterinary diagnostic investigation》2001,13(1):26-29

A total of 720 Escherichia coli strains isolated from diarrheic piglets on 756 swine farms were screened for the presence of the enteroaggregative E. coli heat-stable enterotoxin 1 (EAST1) gene by polymerase chain reaction (PCR). Escherichia coli strains that carried EAST1 genes were also tested by PCR for the presence of 4 fimbriae (F4, F5, F6, F41), 2 heat-stable enterotoxins (STa and STb), and 1 heat-labile enterotoxin (LT) gene. One hundred sixty-four (22.7%) of the 720 E. coli isolates carried genes for EAST1. Of these 164 isolates, 62 (37.8%) carried EAST1 genes only, 11 (6.7%) carried genes for at least 1 of the fimbrial adhesins, 51 (31.1%) carried genes for at least 1 of the enterotoxins, and 40 (23.8%) carried genes for at least 1 of the fimbrial adhesins and enterotoxins. Forty-six percent of strains that carried EAST1 genes carried STa genes, and 16% of strains that carried EAST1 genes carried F4. The isolation rate of enterotoxigenic E. coli strains carrying genes for EAST1 gene was 63%. The 6 major genotypes observed in this study (in decreasing order) were EAST1+, EAST1+STa+, EAST1+STa+STb+, EAST1+STa+F5+, EAST1+STa+F4+, and EAST1+STb+F4+. EAST1 is widely prevalent among diarrheagenic strains of E. coli and may represent an important virulence determinant in the pathogenesis of enteric colibacillosis of preweaned pigs.  相似文献   

大蒜根蛆的发生规律及防治技术   总被引：2，自引：0，他引：2  

郑露  李国敬  关银池  刘绪红  易行荣  黄俊斌 《湖北农业科学》2008,47(8)

对湖北省当阳市大蒜根蛆的发生规律、不同品种的抗虫性及药剂防治进行了调查试验。结果表明，大蒜根蛆在湖北1年发生2代，第1代根蛆危害时期为9月8～26日，第2代为9月25日至10月20日，第2代根蛆发生量大，危害严重；不同大蒜品种根蛆危害差异不大；药剂防治以10％地友GR处理土壤防效较好，为88.8％，叶面喷雾以2.5％肯打EC防效较好，为77.7％。  相似文献   

木质素基超塑化剂的制备及其在砂浆中的应用     

魏民  易聪华  邓永红  杨东杰  邱学青 《林产化学与工业》2009,29(6):29-37

来源于木材、麦草、竹子和蔗渣的木质素磺酸钠与三聚氰胺、甲醛和焦亚硫酸钠合成出磺化木质素三聚氰胺甲醛超塑化剂(LSMF).系统研究了LSMF对砂浆减水率、抗压强度、抗折强度和加速碳化深度等应用性能的影响.结果表明,使用麦草基木质素磺酸钠合成出磺化度3.663mmol/g,特性黏度7.24mL/g的LSMF对砂浆的减水增强性能最好.当LSMF掺量为0.7%时,砂浆减水率为21.1%.硬化砂浆28d加速碳化深度为13mm,空白砂浆为34.5mm.掺加LSMF后,硬化砂浆28d抗压强度和抗折强度分别比空白砂浆提高37%和18%.LSMF超塑化剂为木质素高效利用提供了一种新的思路.  相似文献   

The Effect of Exogenous Hormone Treatment on Spermiation in Rhynchocypris oxycephalus (Sauvage and Dabry)     

I.-S. Park  G. C. Choi  D. S. Kim  Y. K. Nam 《Journal of the World Aquaculture Society》2002,33(4):494-500

For the evaluation of hormonal control of spermiation in fish, a method to quanify the spermiation response of mature Rhynchocypris oxycephalus (Sauvage and Dabry) to hormonal therapy is described. Spermatocrit was determined after 7 min centrifugation at 18,000 ± g and sperm density was estimated by a standard hemocytomer method. Sperm density can be predicted from spermatocrit since their relationship is linear as described by the regression equation, Y = 3.68X - 27.18 ( R ² = 0.82, N = 50). where Y is spermatocrit and × is sperm density. Milt production by mature R. oxycephalus was highest at 24 h after injection of 1,000 IU human chorionic gonadotropin (HCG) and 50 μg luteinizing hormone-releasing hormone analogue (LHRHa) per kg body weight. Increased milt production coincided with low spermatocrit and sperm density levels. These results demonstrate that spermiation in mature R. oxycephalus can be reliably evaluated by a spermatocrit method and that HCG and LHRHa are effective in stimulating of spermiation in this species.  相似文献   

HNS型三相分离器的应用   总被引：1，自引：0，他引：1  

严国民  易南华  肖勇  何新风 《油气储运》2002,21(1):37-39

在分析HNS型三相分离器工作原理及结构的基础上，通过现场实际应用测试，指出该设备油气水分离系统运行平衡，对含水率在80％以上的产出液，经该设备脱气，脱水处理后，原油含水率低于0．38％，水中含油低于300mg/L，完全能达到设计指标。  相似文献   

梨芽变新品种—尖把王梨的选育   总被引：1，自引：0，他引：1  

李俊才  隋洪涛  王家珍  刘成  许英武  闫忠业  孙丽梅  蔡忠民  沙守峰  伊凯 《果树学报》2006,23(6):912-913,F0002

尖把王梨为尖把梨大果芽变,平均单果质量189g,最大单果质量280g,是一般尖把梨的2倍以上。部分果面有1～2条纵沟,果面黄绿色;果皮薄,果肉白色,肉质细腻多汁,石细胞较少;总糖含量为11.88%,总酸含量为0.92%,维生素C含量为5.81mg/100g,风味酸甜适口,香气浓,果心小,可食率高,品质上,是鲜食、冻制和加工兼用新品种。枝条、叶片也明显大于尖把梨,抗寒能力与尖把梨相同,是栽培梨中抗寒力最强的品种之一,适于辽宁、吉林、内蒙古等较寒冷地区发展。  相似文献   

不同环境条件下几种高寒草甸植物的非克隆繁殖投入和生长     

易现峰 《四川草原》2003,(2):13-16

通过对高寒草甸生态系统金露梅灌丛、矮嵩草草甸、围栏矮嵩草草甸以及模拟地球增温效应下5种植物繁殖投入和生长特性的测定,结果表明,矮嵩草、麻花艽、苔草以及美丽风毛菊的叶长沿对照矮嵩草草甸、0TC、围栏以及金露梅灌丛呈现上升趋势,只是灌丛中苔草和麻花艽两种植物的叶长较围栏和OTC有所下降,而环境变化对植物的叶宽的影响较小。苔草在金露梅灌丛中的繁殖投入最大,其次是矮嵩草草甸和围栏,OTC中苔草的繁殖投入几乎为零。围栏与矮嵩草草甸内苔草的繁殖投入差异不显著,而两者中植物的繁殖投入与金露梅灌丛相比,差异性显著(P<0.05)。矮火绒草在矮嵩草草甸中的繁殖投入最大,其次是0Tc和围栏,灌丛中矮火绒草的繁殖投入最小。OTC与矮嵩草草甸内矮火绒草的繁殖投入差异不显著,而OTC与围栏中矮火绒草的繁殖投入差异性显著(P<0.05)。由此我们可以得出一个结论:繁殖投入随环境梯度的变化,反映了植物繁殖对策和繁殖投入在整个生活史特征中的可塑性。  相似文献