Specificities of antinuclear antibodies detected in dogs with systemic lupus erythematosus   总被引：4，自引：0，他引：4  

O Costa  C Fournel  E Lotchouang  J C Monier  M Fontaine 《Veterinary immunology and immunopathology》1984,7(3-4):369-382

Five hundred and eighty dogs with at least one clinical sign compatible with a systemic lupus erythematosus (SLE) were entered in a prospective study aimed at evaluating the prevalence of antinuclear antibodies (ANAb). SLE was diagnosed in 38 of these dogs (group A) which fulfilled at least four American Rheumatism Association (ARA) criteria; of these, sixteen had ANAb titers greater than or equal to 4096. The 23 dogs which met three or two ARA criteria (group B) had an ANAb geometric mean titer (GMT) of 259. Dogs (group C) with only 1 criterium had an ANAb GMT of 75. Anti-ds-DNA Ab were present in 6 dogs from group A (16%), and 2 dogs from group B (9%). Anti-histone Ab were present among dogs from group A, B and C with frequencies of 81%, 67% and 26%, respectively. Among dogs from group A, the ANAb titers and the levels of anti-histone Ab correlated positively when individual sera were considered. Antibodies against the soluble nuclear antigen (SNA) were detected in 74%, 39% and 13% of the dogs from groups A, B and C, respectively. Antibodies initially described in human SLE also exist in SLE dogs. Anti-Sm Ab were found in 24% of dogs in group A. With anti-RNP Ab the frequency was still lower (10%). However, two other types of anti-SNA Ab against RNAse and trypsin-resistant antigens, not found in human "reference sera", were often detected. The first type (anti-type 1 Ab) was found in 26% and 9% of group A and group B, The first type (anti-type 1 Ab) was found in 26% and 9% of group A and group B, respectively; the second type (anti-type 2 Ab) is less frequent, and was found in 13% and 17% of group A and B, respectively. It appears that testing for anti-Sm, anti-type 1 and anti-histone Ab should be performed in order to improve the diagnosis of SLE in dogs.  相似文献   

Fine needle aspiration biopsy of malignant tumors in dogs and cats: a report of 102 cases          下载免费PDF全文

Ménard M  Fontaine M  Morin M 《The Canadian veterinary journal. La revue veterinaire canadienne》1986,27(12):504-510

Fine needle aspiration biopsy is a fast and inexpensive technique well tolerated by animals. Its efficacy for the diagnosis of malignancy and determination of cellular origin was investigated for 97 malignant tumors and five benign lesions in 83 dogs and 19 cats. Histological examination of the lesions was performed in each case. Malignancy was detected by cytology in 69% of the malignant tumors. The cellular origin of the lesion was determined in 74% of the cases. Confirmation of malignancy and determination of cellular origin permitted an early diagnosis and prognosis. Since fine needle aspiration biopsy and cytology cannot definitely rule out malignancy, a surgical biopsy and histopathology should be done when malignancy is suspected clinically and not confirmed cytologically. Specimens of good quality, adequate collaboration between the cytologist and clinicians, and sufficient knowledge of pathology and cytology are basic requirements for obtaining good results with fine needle aspiration biopsy and cytology.  相似文献   

Exposure to Temperature and Insecticides Modulates the Expression of Small Noncoding RNA-Associated Transcripts in the Colorado Potato Beetle,Leptinotarsa decemlineata (Coleoptera: Chrysomelidae)     

Mariem Ben Youssef  Brigitte Christelle Oudraogo  Pierre Bastarache  Pascal Dumas  Chandra E Moffat  Jessica L Vickruck  Pier Jr Morin 《Journal of insect science (Online)》2022,22(1)

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The application of tannic acid to the elimination of egg stickiness at varied moments of the egg swelling process in pikeperch,Sander lucioperca (L.)          下载免费PDF全文

Daniel Żarski  Sławomir Krejszeff  Dariusz Kucharczyk  Katarzyna Palińska‐Żarska  Katarzyna Targońska  Krzysztof Kupren  Pascal Fontaine  Patrick Kestemont 《Aquaculture Research》2015,46(2):324-334

The aim of this article was to analyse the process of pikeperch, Sander lucioperca, egg swelling and to apply tannic acid to eliminate egg stickiness at different moments of the swelling process on artificially obtained eggs. The first experiment involved observation of egg swelling process and the second determined the effect of temperature (12, 14 and 16°C) on the egg swelling rate. The third experiment involved elimination of egg stickiness in a tannin solution (0.75 g L^?1) where eggs were submerged in a solution for 0.5, 1, 2 and 5 min – 5, 10, 15, 20, 25 and 30 min following gamete activation. The results indicate that the pikeperch egg swelling process lasts 30 min. It was found that the temperature did not affect the process duration. The results of the third experiment showed that the effectiveness of tannic acid application in egg stickiness elimination increases with time. The best result was obtained in groups of eggs submerged for 1 and 2 min (86.5% and 80.5% of larvae were obtained respectively) 30 min following the gamete activation. The results presented in this study for the first time indicate the possibility of highly effective procedure of egg stickiness elimination with tannic acid in pikeperch aquaculture.  相似文献   

Off-Season Spawning of Eurasian Perch Perca Fluviatilis     

Herve Migaud  Jean-Noel Gardeur  Patrick Kestemont  Pascal Fontaine 《Aquaculture International》2004,12(1):87-102

This study was designed to determine the effects of the selection for body weight and of two final warming durations during an out-of-season reproductive cycle on gonad recrudescence, plasma steroid levels and spawning in Eurasian perch Perca fluviatilis males and females. Two different bodyweight classes (200–250 and 350–400 g) and two different warming periods (1 or 2 months) were studied.At the end of the chilling period, a significant effect of the selection for body weight was observed on gonad development whereas fish were the same age. According to higher levels in testosterone (12 ng mL^–1), a better development was recorded for smaller breeders (P < 0.05). A short water warming period up to 14°C (1 month v.s. 2 months) allowed higher rates of females with completed gonadogenesis (33–38% v.s. 7–8%) and spawning (31–33% v.s. 0%) (P < 0.05). Spontaneous out-of-season spawning (17 ribbons) and larvae were obtained. Relative fecundity was about 100 eggs g^–1 of female body weight. Five ribbons were partly fertilized with a rate ranging from 5 to 90%. These studies have shown that delayed spawning in Eurasian perch can be obtained by a temperature manipulation, but results are not sufficient because of low stimulation rate of females, low spawning rates and high heteroge-neity of fertilization rates. The effects of other environmental factors like photoperiod should be tested in further experiment to improve the protocol inducing out-of-season spawning production.  相似文献   

Odor Modification in Salmon Hydrolysates Using the Maillard Reaction     

Christelle Kouakou  Jean-Pascal Bergé  Régis Baron  Laurent Lethuaut  Carole Prost 《Journal Of Aquatic Food Product Technology》2013,22(5):453-467

The aim of this work was to study the effect of adding sugar during proteolysis to promote the Maillard reaction and mask the initial fish odor and off-flavors generated. An experimental design, based on the Doehlert plan, was used to study the influence of hydrolysis conditions (time, temperature, sugar, and antioxidant addition) on the odor characteristics of hydrolysates, soluble protein levels, and amino acid content. Results showed that the lowest level of sugar (10 g of D-xylose added to 1 kg of by-products) was enough to develop a grilled odor in hydrolysates. In the hydrolysis conditions used—i.e., enzyme inactivation at 95°C for 30 min—hydrolysis temperature had no effect on grilled odor production but significantly affected the soluble protein fraction, as did hydrolysis time. Soluble protein content and essential amino acid content increased with the enzymatic reaction but were not modified by adding sugar. Hydrolysis conditions that promote Maillard reactions while keeping a nutritional balance have been identified.  相似文献   

Investigation of the sensitivity of Plasmopara viticola to amisulbrom and ametoctradin in French vineyards using bioassays and molecular tools     

Sverine Fontaine  Florent Remuson  Laëtitia Caddoux  Benoit Barrs 《Pest management science》2019,75(8):2115-2123

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Direct impacts of recent climate warming on insect populations     

Christelle ROBINET  Alain ROQUES 《Integrative zoology》2010,5(2):132-142

Effects of recent climate change have already been detected in many species, and, in particular, in insects. The present paper reviews the key impacts of global warming on insect development and dispersal. The effects of climate change appear to be much more complex than a simple linear response to an average increase in temperature. They can differ between seasons and bioclimatic regions. Earlier flight periods, enhanced winter survival and acceleration of development rates are the major insect responses. Differential response of insects and hosts to warming up might also lead to disruption of their phenological synchrony, but adaptive genetic processes are likely to quickly restore this synchrony. In a number of cases, warming results in removing or relocating the barriers that limit present species' ranges. It is also likely to facilitate the establishment and spread of invasive alien species. Finally, knowledge gaps are identified and future research interests are suggested.  相似文献   

Expression of interleukin-1beta in the digital laminae of horses in the prodromal stage of experimentally induced laminitis     

Fontaine GL  Belknap JK  Allen D  Moore JN  Kroll DL 《American journal of veterinary research》2001,62(5):714-720

OBJECTIVE: To study expression of interleukin-1beta (IL-1beta) in the digital laminae of horses in the prodromal stage of experimentally induced laminitis. ANIMALS: 8 healthy adult horses with no signs of laminitis. PROCEDURES: Black walnut extract was administered via nasogastric tube to 4 horses, and water was administered to the remaining 4 (controls). Complete blood counts and physical examinations were performed every 30 minutes after administration of black walnut extract or water. General anesthesia was induced when total WBC count decreased by 30% in horses given the black walnut extract and 3 hours after water administration in control horses. The left forefoot was perfusion fixed with neutral-buffered 10% formalin, and paraffin-embedded sections of the digit were used for in situ hybridization with an equine-specific IL-1beta probe. RESULTS: IL-1beta mRNA expression was observed in perivascular cells of the small laminar venules and capillaries in all 4 horses given black walnut extract and in interstitial cells remote from the microvasculature in 1 of the 4. Other cellular components of the laminar tissue and cellular components of the digital arterioles and veins did not exhibit IL-1beta mRNA expression. Expression of IL-1beta mRNA was not detected in laminae from control horses. CONCLUSIONS AND CLINICAL RELEVANCE: Results suggest that IL-1beta mRNA is expressed by perivascular cells in the laminar tissues of horses in the prodromal stage of experimentally induced laminitis. This provides evidence of an inflammatory process during the prodromal stage of laminitis, indicating that local digital proinflammatory cytokine expression may be an initiating factor in laminitis.  相似文献   

Evaluation of a rapid method of determination of plasma fibrinogen in swine.          下载免费PDF全文

M Fontaine  B J McSherry    V E Valli 《Canadian journal of veterinary research》1977,41(2):202-205

An evaluation was made of a rapid semiautomated method for determining fibrinogen level in swine plasma. This method, referred to as thrombin time method or fibrometer method, is based on the principle that when thrombin is added to suitably diluted plasma, the time of clotting is linearly related to the fibrinogen concentration. The linear regression model for the standard curve prepared using swine plasma had an r value of 0.998. A comparison between the fibrometer and the Grannis methods done on 189 swine plasma samples showed good correlation between these two mehtods (r value 0.847). It was concluded that although the fibrometer method may not be as precise as the Grannis method, it would still be acceptable for clinical use in swine.  相似文献