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Bengochea P Christiaens O Amor F Viñuela E Rougé P Medina P Smagghe G 《Pest management science》2012,68(7):976-985
BACKGROUND: The moulting accelerating compounds (MACs) or ecdysteroid agonists represent a selective group of insecticides acting upon binding to the ecdysteroid receptor (EcR) and leading to lethal premature moulting in larval stages and aborted reproduction in adults. Psyttalia concolor Szèpl. is a useful parasitic wasp attacking important tephritid pests such as the medfly and olive fruit fly. RESULTS: Contact and oral exposure in the laboratory of female parasitic wasps to the dibenzoylhydrazine‐based methoxyfenozide, tebufenozide and RH‐5849 did not provoke negative effects. No mortality and no reduction in beneficial capacity were observed. The ligand‐binding domain (LBD) of the EcR of P. concolor was sequenced, and a homology protein model was constructed which confirmed a cavity structure with 12 α‐helices, harbouring the natural insect moulting hormone 20‐hydroxyecdysone. However, a steric clash occurred for the MAC insecticides owing to a restricted extent of the ligand‐binding cavity of the PcLBD‐EcR, while they did dock well in that of susceptible insects. CONCLUSIONS: The insect toxicity assays demonstrated that MACs are selective for P. concolor. The modelling/docking experiments are indications that these insecticides do not bind with the LBD‐EcR of P. concolor and support the theory that they show no biological effects in the parasitic wasp. These data may help in explaining the compatible use of MACs together with parasitic wasps in IPM programmes. Copyright © 2012 Society of Chemical Industry 相似文献
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C. Vaneeckhaute E. Meers E. Michels P. Christiaens F. M. G. Tack 《Water, air, and soil pollution》2012,223(4):1593-1603
In the transition from a fossil to a bio-based economy, it has become an important challenge to maximally recuperate and recycle
valuable nutrients coming from manure and digestate processing. Membrane filtration is a suitable technology to separate valuable
nutrients in easily transportable concentrates which could potentially be re-used as green fertilizers, in the meantime producing
high quality water. However, traditional membrane filtration systems often suffer technical problems in waste stream treatment.
The aim of this study was to evaluate the performance of vibratory shear enhanced processing (VSEP) in the removal of macronutrients
(N, P, K, Na, Ca, Mg) from the liquid fraction of digestates, reducing their concentrations down to dischargeable/re-usable
water. In addition, the re-use potential of VSEP-concentrates as sustainable substitutes for fossil-based mineral fertilizers
was evaluated. Removal efficiencies for N and P by two VSEP filtration steps were high, though not sufficient to continuously
reach the Flemish legislation criteria for discharge into surface waters (15 mg N l−1 and 2 mg P l−1). Additional purification can occur in a subsequent lagoon, yet further optimization of the VSEP filtration system is advised.
Furthermore, concentrates produced by one membrane filtration step showed potential as N–K fertilizer with an economic value
of €6.3 ± 1.1 t−1 fresh weight (FW). Further research is, however, required to evaluate the impact on crop production and soil quality by application
of these new potential green fertilizers. 相似文献
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Ye Chao Wang Zheng-Wu Sheng Yuan-Lan Wang Zi-Guo Smagghe Guy Christiaens Olivier Niu Jinzhi Wang Jin-Jun 《Journal of pest science》2022,95(1):87-100
Journal of Pest Science - Gram-negative binding proteins (GNBPs) are important in the innate immune system of insects in recognition of fungi pathogen, such as Beauveria bassiana. However, this... 相似文献
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RNA interference technology in crop protection against arthropod pests,pathogens and nematodes 
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下载免费PDF全文 Moises Zotti Ericmar Avila dos Santos Deise Cagliari Olivier Christiaens Clauvis Nji Tizi Taning Guy Smagghe 《Pest management science》2018,74(6):1239-1250
Scientists have made significant progress in understanding and unraveling several aspects of double‐stranded RNA (dsRNA)‐mediated gene silencing during the last two decades. Now that the RNA interference (RNAi) mechanism is well understood, it is time to consider how to apply the acquired knowledge to agriculture and crop protection. Some RNAi‐based products are already available for farmers and more are expected to reach the market soon. Tailor‐made dsRNA as an active ingredient for biopesticide formulations is considered a raw material that can be used for diverse purposes, from pest control and bee protection against viruses to pesticide resistance management. The RNAi mechanism works at the messenger RNA (mRNA) level, exploiting a sequence‐dependent mode of action, which makes it unique in potency and selectivity compared with conventional agrochemicals. Furthermore, the use of RNAi in crop protection can be achieved by employing plant‐incorporated protectants through plant transformation, but also by non‐transformative strategies such as the use of formulations of sprayable RNAs as direct control agents, resistance factor repressors or developmental disruptors. In this review, RNAi is presented in an agricultural context (discussing products that have been launched on the market or will soon be available), and we go beyond the classical presentation of successful examples of RNAi in pest‐insect control and comprehensively explore its potential for the control of plant pathogens, nematodes and mites, and to fight against diseases and parasites in beneficial insects. Moreover, we also discuss its use as a repressor for the management of pesticide‐resistant weeds and insects. Finally, this review reports on the advances in non‐transformative dsRNA delivery and the production costs of dsRNA, and discusses environmental considerations. © 2017 Society of Chemical Industry 相似文献
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Benigna Van Eynde David Vuylsteke Olivier Christiaens Kris Cooreman Guy Smagghe Daan Delbare 《Aquaculture Research》2019,50(6):1658-1667
The European brown shrimp, Crangon crangon, is a highly valued commercial species in Europe. These shrimp fisheries are characterized by strong seasonal variations in average landings. Attempts to ‘catch and hold’ wild adults in land‐based rearing systems have been proven to be very difficult, due to inadequate feed, slow growth and high mortality. In this study, we have optimized design and operation procedures of a small‐scale static larval rearing system for the culture of C. crangon larvae. Focus was on optimizing larval survival via water temperature, feed selection, feeding regime and density. This is the first report that shows that C. crangon larvae can be reared at high densities of 300 larvae L‐1 with high survival of 73.5 (± 5.4)% under laboratory conditions. In these systems, larvae can be fed exclusively Artemia nauplii according to a feeding regime which is adjusted based on major moulting events. Addition of microalgae may further increase survival by 10%. The information gathered during this research can be applied to further optimize larval development in either flow‐through or recirculation systems. 相似文献