Soil organic carbon allocation and dynamics under perennial energy crops and their feedbacks with soil microbial biomass and activity     

Thorsten Ruf  Christoph Emmerling 《Soil Use and Management》2020,36(4):646-657

The cultivation of perennial instead of annual energy crops has received growing interest. Previous studies identified numerous beneficial effects of perennial energy crop (PEC) cultivation for the agricultural landscape such as promotion of agrobiodiversity, reduced requirements for agrochemicals and fertilizers as well as a large potential for carbon accumulation in soil. However, the mere presence of soil organic matter (SOM) accumulation gives no indication about the persistence of the SOM for example after a recultivation of the stands. Therefore, this study focused on SOM pools of different density fractions and soil microbial parameters. Six different PECs were tested against a typical benchmark system as feedstock for anaerobic digestion. The study has shown that all PEC species increased soil microbial activity and provided an insight how they sequester carbon in soil. Moreover, significant modifications in basic soil properties caused by plant growth were observed. For example, the cultivation of giant knotweed has lowered the soil pH by more than 0.5 pH units compared to the benchmark system. After 5 years of PEC cultivation, total soil organic carbon stocks were increased between 1,500 ± 400 and 4,500 ± 1,500 kg C ha^-1 for the upper 10 centimetres of soil. The distribution among different soil fractions showed species-specific patterns. Tall wheatgrass and Virginia mallow showed particular high accumulation rates in the mineral-associated SOM fraction which indicates long residence times of the SOM after a possible recultivation of the fields.  相似文献   

Upper body movement analysis of multiple limb asymmetry in 367 clinically lame horses     

Suphannika Phutthachalee  Kathrin Mählmann  Suvaluk Seesupa  Christoph Lischer 《Equine veterinary journal》2021,53(4):701-709

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Investigation of a possible chain of Streptococcus suis infection in a pig breeding community using PCR-based genotyping     

Neis C  Rohde J  Valentin-Weigand P  Baums CG 《Berliner und Münchener tier?rztliche Wochenschrift》2007,120(5-6):202-206

Streptococcus (S.) suis plays an important role in pig breeding causing invasive diseases such as meningitis and polyarthritis. Herd problems with Streptococcus suis are common in many pig farms and are frequently characterised by disease outbreaks. In this context, it is often important to identify chains of infection, e.g. between a farrow-to-wean and a grower farm. In the following case report a possible chain of infection among the different farms of a farrow-to-finish system was investigated. In two grower units herd problems with S. suis were present; the mortality was higher than 5 %. It appeared likely, that the streptococci causing these problems were transmitted from a single farrow-to-wean unit to the two different grower farms. In the respective farms swabs were taken from different healthy animals and, in the case of the grower farms, also from the infected animals. Genotypic profiling of strains by a multiplex-PCR and AFLP typing method revealed that two different S. suis pathotypes were responsible for the herd problems. Both pathotypes could not be detected in the farrow-to-wean farm.Thus, a chain of infection originating from the farrow-to-wean farm appeared unlikely. The multiplex PCR was in this case sufficient to elucidate the described problem.  相似文献   

Diagnosis and outcome following tenoscopic surgery of the digital flexor tendon sheath in German sports and pleasure horses     

Andrea N. Cender  Kathrin Mählmann  Anna Ehrle  Roswita Merle  Laura Pieper  Christoph J. Lischer 《Equine veterinary journal》2023,55(1):48-58

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Structures and possibilities of herd management on the example of respiratory enzootic infection of calves     

Hofmann W  Heckert HP 《Berliner und Münchener tier?rztliche Wochenschrift》1999,112(12):430-434

With the keeping of cattle in bigger herds problems arise, which require a modified veterinary approach. In stead of and in addition to the treatment of individual animals improvement of hygiene, intensification of prophylaxis, fast recognition (herd diagnostics) and interregional comparability have to be established. Beside the conventional field veterinarian, who is consulted on request primarily for the treatment of single animals, specialists in team practices as an alternative, permanently employed veterinarians or firm contracts with a health service up to integrated herd control are thinkable. The clarification and control of infectious diseases of the respiratory tract in cattle operations require an exact analysis of the environmental conditions and the identification of etiologic agents. Special attention has to be drawn to the evaluation of the barn climate and the respective taking of samples concerning the actual disease situation.  相似文献   

Classical swine fever in wild boars in Switzerland     

Hofmann MA  Thür B  Vanzetti T  Schleiss W  Schmidt J  Griot C 《Schweizer Archiv für Tierheilkunde》1999,141(4):185-190

In May 1998, wild boars with classical swine fever (CSF) symptoms were detected in the southern part (Canton Ticino) of Switzerland. CSF virus was isolated from the submitted samples and RT-PCR followed by direct nucleotide sequencing of the 5' non-translated region showed that this virus was identical to the isolate previously recognized in wild boars from the area of Varese (Italy). In most animals, antibodies to CSF virus were detected as well. An immediate measurement was taken by limiting the movement of pigs and identifying both risk and surveillance zones. In order not to disturb potentially infected wild boars within their habitat a complete hunting prohibition for 2 months was enforced. The different possibilities of the control of CSF outbreaks in wild boars are discussed.  相似文献   

A survey of Newcastle disease in Swiss laying-hen flocks using serological testing and simulation modelling   总被引：1，自引：0，他引：1  

Gohm DS  Thür B  Audigé L  Hofmann MA 《Preventive veterinary medicine》1999,38(4):459-288

Newcastle disease (ND) is a highly contagious viral disease of birds particularly domestic poultry. Switzerland is currently declared free from ND; since vaccination is prohibited, the detection of antibodies against ND virus (NDV) results in the destruction of the respective flock (stamping-out policy). However, in 1995 and 1996, antibody-positive flocks were detected and sporadic ND outbreaks even occurred in Switzerland. Therefore, a serosurvey was done to look for evidence of NDV infections in Swiss laying-hen flocks. The survey was designed to provide 95% confidence of detecting at least one seropositive flock if the flock prevalence were 1%. Thirty blood samples from each of 260 commercial laying-hen flocks were collected during 1996 in a central poultry slaughterhouse. Sera were screened for NDV antibodies with a commercial blocking enzyme-linked immunosorbent assay (ELISA). Samples with a questionable or positive test result were retested with the same ELISA. A stochastic computer model was applied to define a cut-off number of test-positive samples to help to differentiate between true- and false-positive flocks and to estimate the true flock prevalence of infection. Four flocks were identified as NDV-seropositive and the NDV true seroprevalence among commercial laying-hen flocks in Switzerland was most likely between 1.35 and 1.55%. This indicates that Swiss laying-hen and parental flocks with more than 150 animals have been in contact with strains of NDV that cause subclinical infection in chicken, because no clinical symptoms have been observed. In this context, computer simulation was a useful technique to interpret survey results.  相似文献   

Studies of Coxiella burnetii infections in dairy herds with special regard to infections in men     

Sting R  Kopp J  Mandl J  Seeh C  Seemann G  Kimmig P  Schmitt K  Mentrup T 《Berliner und Münchener tier?rztliche Wochenschrift》2002,115(9-10):360-365

Investigations of 1167 dairy cows out of 105 herds with fertility disorders on secretion of Coxiella burnetii (C. burnetii) by the genitals as well as serological studies of these animals using ELISA demonstrated that C. burnetii infections are significantly associated with abortions but not with repeated inseminations without success or vaginal excretions. The demonstration of an increased risk of infections for persons caring for those dairy herds could be shown by a total of 253 serological studies. A comparison of these studies of farmers caring for dairy herds suffering from abortions a seroprevalence of > or = 20% showed that these persons revealed significantly more frequent antibodies against C. burnetii than farmers of the group compared with. Further studies showed that in herds suffering from abortions a seroprevalence of > or = 20% means an additional risk of infections of farmers. Vice versa abortions of the cows in herds with a seroprevalence of > or = 20% imply an additional infectious risk.  相似文献   

A novel fluorescent pH probe for expression in plants     

Alexander Schulte  Inken Lorenzen  Markus Böttcher  Christoph Plieth 《Plant methods》2006,2(1):7-13

Background  

The pH is an important parameter controlling many metabolic and signalling pathways in living cells. Recombinant fluorescent pH indicators (pHluorins) have come into vogue for monitoring cellular pH. They are derived from the most popular Aequorea victoria GFP (Av-GFP). Here, we present a novel fluorescent pH reporter protein from the orange seapen Ptilosarcus gurneyi (Pt-GFP) and compare its properties with pHluorins for expression and use in plants.  相似文献   

Enzyme-linked immunosorbent assay for the detection of porcine epidemic diarrhea coronavirus antibodies in swine sera   总被引：1，自引：0，他引：1  

M Hofmann  R Wyler 《Veterinary microbiology》1990,21(3):263-273

An enzyme-linked immunosorbent assay (ELISA) for detecting serum antibodies to the porcine epidemic diarrhea coronavirus (PEDV) was established by using cell culture-grown PEDV as antigen for coating. Ultracentrifugation through 20 and 45% (w/w) sucrose cushions proved to be the best antigen purification method. Examination of 1024 swine sera showed a high specificity and a greater sensitivity of the ELISA, when compared with indirect immunofluorescence. Reference sera with high antibody titers to PEDV originated from two pigs experimentally infected with PEDV. Three different antigen purification methods and the advantages of the ELISA compared with an immunofluorescence test are discussed.  相似文献