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1.
Field trial of a staphylococcal mastitis vaccine in dairy herds: clinical, subclinical and microbiological assessments 总被引：4，自引：0，他引：4
Objective To assess the efficacy of a new staphylococcal mastitis vaccine under commercial dairying conditions.
Design A field trial involving 1819 cows and heifers conducted on seven dairy herds in Victoria. The trial was done 'blind'; approximately half the animals were vaccinated and the remainder were untreated controls.
Procedure The vaccine was given twice during the last 10 weeks of pregnancy. Effects of vaccination were assessed, during the ensuing lactation, on the basis of clinical and sub-clinical mastitis and microbiological investigations of the milk.
Results A total of 273 cases of clinical mastitis were recorded. Staphylococcus aureus was isolated from 112 of these, 45 cases in vaccinates and 67 cases in controls; the difference was not statistically significant. One herd was notable in having a high incidence of clinical staphylococcal mastitis. This herd accounted for 15.8% of the animals in the field trial but 54.5% of cases of clinical staphylococcal mastitis. For this herd, vaccinated animals had significantly lower incidence of clinical staphylococcal mastitis and prevalence of subclinical mastitis, relative to controls. An unexpected feature of the trial as a whole was the low incidence of clinical mastitis from which S aureus was isolated in pure culture (26.3% of cases) and the high incidence of clinical Streptococcus uberis mastitis (22.7% of cases).
Conclusions The trial showed that the vaccine was efficacious in reducing the incidence of clinical mastitis and prevalence of subclinical mastitis in a herd that had a serious staphylococcal mastitis problem. 相似文献
Design A field trial involving 1819 cows and heifers conducted on seven dairy herds in Victoria. The trial was done 'blind'; approximately half the animals were vaccinated and the remainder were untreated controls.
Procedure The vaccine was given twice during the last 10 weeks of pregnancy. Effects of vaccination were assessed, during the ensuing lactation, on the basis of clinical and sub-clinical mastitis and microbiological investigations of the milk.
Results A total of 273 cases of clinical mastitis were recorded. Staphylococcus aureus was isolated from 112 of these, 45 cases in vaccinates and 67 cases in controls; the difference was not statistically significant. One herd was notable in having a high incidence of clinical staphylococcal mastitis. This herd accounted for 15.8% of the animals in the field trial but 54.5% of cases of clinical staphylococcal mastitis. For this herd, vaccinated animals had significantly lower incidence of clinical staphylococcal mastitis and prevalence of subclinical mastitis, relative to controls. An unexpected feature of the trial as a whole was the low incidence of clinical mastitis from which S aureus was isolated in pure culture (26.3% of cases) and the high incidence of clinical Streptococcus uberis mastitis (22.7% of cases).
Conclusions The trial showed that the vaccine was efficacious in reducing the incidence of clinical mastitis and prevalence of subclinical mastitis in a herd that had a serious staphylococcal mastitis problem. 相似文献
2.
研究优质蛋白玉米籽粒品质性状组间典型相关性结果表明,2个半硬质胚乳奥帕克-2玉米群体(“中群14QPMC3”和“陕综5号QPMC0”)籽粒物理性状中硬质度、籽粒密度、百粒重与胚比、百粒体积呈负相关效应,营养品质中赖氨酸含量、蛋白质品质性状为其核心,籽粒密度(或硬质度)可作为蛋白质品质的间接选择指标;籽粒物理性状、蛋白质品质性状在赖氨酸产量构成中起决定性作用,全籽粒蛋白质品质与胚乳蛋白质品质紧密相关,而与胚蛋白质品质无关,高赖氨酸高油分育种相统一。 相似文献
3.
Detlef Deumlich R Funk J Kiesel HI Reuter J Thiere L Völker 《Archives of Agronomy and Soil Science》2013,59(3):259-271
This paper presents a comparative method (VERMOST) to evaluate complex site conditions at the level of a federal state (Brandenburg) and at different areal units. The methodology uses primary site conditions and provides the possibility to objectively compare and judge different ecological questions. Objects with heterogeneous content are pooled in comparison groups. Considering the main site characteristics allows a goal-oriented allocation of subsidies. The use of VERMOST is demonstrated for the wind and water erosion risk assessment across different scales from a federal state down to a parcel of land. The ‘parcel’ aggregation-level proves to be useful to assess the erosion risk. Larger scales are less useful in the assessment of subsidies policy with regard to erosion risk. A moving-window-technique combined with VERMOST is demonstrated to be suitable for comparing and assessing regional aspects more objectively compared with data administratively linked. 相似文献
4.
In order to improve general adaptive capability of algorithm,the new color image segmentation algorithm based on feature divergence and fuzzy theory(FDCIS) is proposed.The algorithm introduces feature divergence and fuzzy dissimilarity function into calculation in order to measure the dissimilarity of feature vector,clusters data by means of feature divergence,and accomplishes the merge of image region.The experimental results demonstrate that the color image segmentation result of the proposed approach reduce calculation on large sample of color image,simply and effectively solve over-segmentation of color image,avoid the dependence of the algorithm on initial condition,and hold favorable consistency in terms of human perception. 相似文献
5.
Kalliopi Kadoglidou Anastasia Lagopodi Katerina Karamanoli Despoina Vokou George A. Bardas George Menexes Helen-Isis A. Constantinidou 《European journal of plant pathology / European Foundation for Plant Pathology》2011,130(3):297-309
The effect of essential oils and individual monoterpenoids on soil-borne fungi, in pure and mixed cultures, in growth media
and in the soil environment, was investigated. Essential oils were extracted from lavender (Lavandula stoechas), oregano (Origanum vulgare subsp. hirtum), sage (Salvia fruticosa) and spearmint (Mentha spicata). The monoterpenoids tested were fenchone, carvacrol, 1,8-cineole, carvone, α-pinene and terpinen-4-ol. Their effect was examined
on growth and sporulation of Aspergillus terreus, Fusarium oxysporum, Penicillium expansum and Verticillium dahliae isolated from an organic cultivation of tomato. All tested essential oils and individual monoterpenoids inhibited mycelial
growth in all fungi and conidial production in most fungi. The strongest inhibitory activity on mycelial growth was exhibited
by oregano and spearmint oils and by carvacrol and carvone, respectively their main constituents. The inhibitory activity
was clearly fungistatic in A. terreus and F. oxysporum but fungicidal in V. dahliae. On sporulation, clearly stimulatory effects were observed alongside inhibitory ones. Conidial production was always promoted
by α-pinene in P. expansum and by sage oil in F. oxysporum. At certain dosages it was promoted by cineole and carvone in F. oxysporum, and by lavender oil in A. terreus and V. dahliae. Experiments with carvone and carvacrol against mixed fungal cultures in a soil environment showed that V. dahliae was the most sensitive and A. terreus the most tolerant of the four fungi. Our results demonstrate strong but divergent effects and selectivity of action of the
lower terpenoids on fungal strains that can become serious pests of tomato. Of special importance is the complete inhibition
of growth and conidial production of V. dahliae, a pathogen otherwise very resistant to chemical control. 相似文献
6.
HI JANG Min ZOU Ying-bin JIANG Peng XIA Bing Md Ibrahim AO He-jun 《中国农业科学(英文版)》2011,10(10):1537-1544
Chinese super hybrid rice breeding project has developed many new varieties with great yield potential.It is controversial which yield component should be emphasized in super hybrid rice production.The present study was conducted to compare super hybrid rice with common hybrid and super inbred rice and analyze contributions of yield components to grain yield of super hybrid rice under experimental conditions,and evaluate relationships between grain yield and yield components of super hybrid rice in farmer's paddy fields.Field experiments were done in Changsha,Guidong,and Nanxian,Hunan Province,China,from 2007 to 2009.Eight super hybrid varieties,one common hybrid variety,and one super inbred variety were grown in each location and year.Rice production investigation was undertaken in high-yielding (Guidong),moderate-yielding (Nanxian),and low-yielding (Ningxiang) regions of Hunan Province,China,in 2009.Grain yield and yield components were measured in both the field experiments and rice production investigation.Super hybrid rice varieties outyielded common hybrid and super inbred varieties across three locations and years.Yield potential has been increased by 11.4％ in super hybrid rice varieties compared with common and super inbred varieties.The higher yield of super hybrid varieties was attributed to improvement in panicle size.Panicles per m2 had the highest positive contribution to grain yield with the exception under yield level of 10.0 to 12.0 t ha-1,and was positively related to grain yield in farmer's field at all of the high-,moderate-,and low-yielding regions.Our study suggests that panicle per m2 ought to be emphasized in super hybrid rice production. 相似文献
7.
Effects of water stress on phenology, growth, stomatal activity and water status were assessed from April to November 1996 in 2-year-old seedlings of Quercus frainetto Ten. (Quercus conferta Kit.), Quercus pubescens Willd., Quercus macrolepis Kotschy (Quercus aegilops auct.) and Quercus ilex L. growing in containers in northern Greece. All four species developed more than 50% of their total leaf area before the beginning of June--an adaptation to arid climates. Well-irrigated plants tended to develop greater individual leaf area, number of leaves per plant, total plant leaf area, height and root:shoot ratios than water-stressed plants, but the difference between treatments was not significant for any parameter in any species. Quercus macrolepis appeared to be the most drought-tolerant of the four species. It maintained the highest number of leaves of the smallest size and increased the proportion of fine roots during drought. In all species, drought caused significant decreases in stomatal conductance and predawn and midday water potentials from mid-July until the end of August, when the lowest soil water content and highest mean daily air temperatures and midday leaf temperatures occurred; however, the responses were species-specific. Among the four species, Quercus macrolepis sustained the highest stomatal conductance despite very low water potentials, thus overcoming drought by means of desiccation tolerance. Quercus ilex decreased stomatal conductance even before severe water stress occurred, thereby avoiding desication during drought. Quercus pubescens had the highest water potential despite a high stomatal conductance, indicating that its leaf water status was independent of stomatal activity. Quercus frainetto was the least drought-resistant of the four species. During drought it developed very low water potentials despite markedly reduced stomatal aperture. 相似文献
8.
D. JORDAN JW PLANT† HI NICOL‡ TM JESSEP CJ SCRIVENER 《Australian veterinary journal》1996,73(6):211-215
Factors associated with the proportion of sheep cured of virulent footrot after antibiotic treatment were studied in a field trial under dry environmental conditions. From 2 similar flocks, 1091 Merino sheep weighing about 50 kg and infected with virulent footrot received an intramuscular injection of either 12 mL of a mixture of penicillin (250 mg/mL) and streptomycin (250 mg/mL), 6 mL of long acting oxytetracycline (200 mg/mL) or 6 mL of a mixture of lincomycin (50 mg/mL) and spectinomycin (100 mg/mL). Variables that were significantly associated with the proportion of sheep cured were: the type of antibiotic used, the number of feet infected and the flock from which the sheep came. There was an interaction between antibiotic type and number of feet infected and between antibiotic type and flock in association with the proportion of sheep cured. The extent of paring and the occurrence of blowfly strike in footrot lesions treated with diazinon had no significant association with the proportion of sheep cured. 相似文献
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本研究从患急性肝胰腺坏死病(Acute hepatopancreatic necrosis disease,AHPND)的凡纳滨对虾(Litopenaeus vannamei)肝胰腺中分离到一株优势菌,编号为20160303005-1,通过16S rRNA和分子伴侣蛋白groEL基因序列分析,并结合生理生化特征,将该细菌鉴定为副溶血弧菌(Vibrio parahaemolyticus),其血清型为O1:KUT(K untypeable)。基因分析结果显示,该菌株携带可引起对虾AHPND的相关毒力蛋白基因pirA~(VP)和pirB~(VP),但不携带副溶血弧菌临床菌株毒力基因:耐热直接溶血毒素(Thenmostable direct hemolysin,tdh)和相对耐热直接溶血毒素(TDH-related hemolysin,trh)基因。菌株对凡纳滨对虾具有较强的致病性,浸泡感染的半数致死剂量(LD_(50))为7.96×10~3 CFU/ml。对虾急性感染后,6 h肝胰腺颜色变浅,肠胃变空;9 h肝胰腺呈浅白色,萎缩变小。9 h死亡数过半,24 h全部死亡。组织病理学分析显示,感染后对虾肝胰腺小管崩塌,上皮细胞严重脱落,呈现出典型的AHPND病理症状。药敏实验结果显示,该菌对庆大霉素、环丙沙星和头孢他啶等16种药物敏感,对阿莫西林、替卡西林和头孢噻吩等5种药物表现为耐药。上述研究可为该病原的流行病学及药物防控研究提供基本数据。 相似文献