Application of nox-restriction fragment length polymorphism for the differentiation of Brachyspira intestinal spirochetes isolated from pigs and poultry in Australia.     

Kirsty M Townsend  Vo Ngan Giang  Carol Stephens  Paul T Scott  Darren J Trott 《Journal of veterinary diagnostic investigation》2005,17(2):103-109

Sixty-nine intestinal spirochetes isolated from pigs and poultry in eastern Australia were selected to evaluate the effectiveness of a species-specific PCR-based restriction fragment length polymorphism (RFLP) analysis of the Brachyspira nox gene. For comparative purposes, all isolates were subjected to species-specific PCRs for the pathogenic species Brachyspira hyodysenteriae and Brachyspira pilosicoli, and selected isolates were examined further by sequence analysis of the nox and 16S ribosomal RNA genes. Modifications to the original nox-RFLP method included direct inoculation of bacterial cells into the amplification mixture and purification of the PCR product, which further optimized the nox-RFLP for use in a veterinary diagnostic laboratory, producing sufficient product for both species identification and future comparisons. Although some novel profiles that prevented definitive identification were observed, the nox-RFLP method successfully classified 45 of 51 (88%) porcine and 15 of 18 (83%) avian isolates into 5 of the 6 recognized species of Brachyspira. This protocol represents a significant improvement over conventional methods currently used in veterinary diagnostic laboratories for rapid specific identification of Brachyspira spp. isolated from both pigs and poultry.  相似文献   

Synovial cell sarcoma in a dog: A misnomer—Cytologic and histologic findings and review of the literature          下载免费PDF全文

Paola Monti  Darren Barnes  Anna M. Adrian  Roberta Rasotto 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2018,47(2):181-185

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Investigations of selected pathogens among village pigs in Central Papua,Indonesia     

Widi Nugroho  Colin Frank Cargill  I. Made Putra  Roy Neville Kirkwood  Darren John Trott  Siti Isrina Oktavia Salasia  Mitra Slipranata  Michael Philipp Reichel 《Tropical animal health and production》2016,48(1):29-36

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Development of a Novel Medial Synoviocentesis Approach to the Bicipital Bursa in an Equine Cadaver Model          下载免费PDF全文

Milosz Grabski  Jennifer McNamara  Darren Roper  Chris Warren‐Smith  Evita Busschers 《Veterinary surgery : VS》2016,45(4):523-528

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Analysis of flowering,stigmas yield and qualitative traits of saffron (Crocus sativus L.) as affected by environmental conditions     

F. Gresta  G. Avola  G.M. Lombardo  L. Siracusa  G. Ruberto 《Scientia Horticulturae》2009

Saffron is well known for its use as a condiment spice, as a dye and traditional medicine. Saffron is experiencing an increasing interest mainly due to its peculiar and manifold properties of the metabolic pool of its stigmas, mainly crocetin esters and picrocrocin. This species is cultivated in environments with very different climatic conditions and with very different corm rates from place to place, passed down over the centuries. The aim of this study was therefore the evaluation of the influence of rainfall, temperature and corm density on flower phenology, stigmas yield and main compositional characteristics of two saffron corms provenience. Flowering beginning in saffron seems to be influenced by the combination of temperature and soil moisture, whilst its flowering calendar proves independent of corm provenance, environment and plant density. On the contrary, the studied factors exert a strong effect on both total stigmas yield and qualitative characteristics: colder environment resulted in a higher flower production, but lower quality of stigmas. Flower number was positively correlated with the stigmas yield, but negatively with its unitary weight. The content of crocetin esters and picrocrocin has been evaluated according to the spectrophotometric ISO normative, which ranked the samples into three qualitative decreasing categories (I–III). The spectrophotometric data showed positive correlation with the unitary stigmas weight and negative with stigmas production.  相似文献   

Observed flow compensation associated with the MOC at 26.5 degrees N in the Atlantic   总被引：3，自引：0，他引：3  

Kanzow T  Cunningham SA  Rayner D  Hirschi JJ  Johns WE  Baringer MO  Bryden HL  Beal LM  Meinen CS  Marotzke J 《Science (New York, N.Y.)》2007,317(5840):938-941

The Atlantic meridional overturning circulation (MOC), which provides one-quarter of the global meridional heat transport, is composed of a number of separate flow components. How changes in the strength of each of those components may affect that of the others has been unclear because of a lack of adequate data. We continuously observed the MOC at 26.5 degrees N for 1 year using end-point measurements of density, bottom pressure, and ocean currents; cable measurements across the Straits of Florida; and wind stress. The different transport components largely compensate for each other, thus confirming the validity of our monitoring approach. The MOC varied over the period of observation by +/-5.7 x 10(6) cubic meters per second, with density-inferred and wind-driven transports contributing equally to it. We find evidence for depth-independent compensation for the wind-driven surface flow.  相似文献   

Temperature effects on the action of acylurea insecticides against tobacco hornworm (Manduca sexta) larvae     

Darren R. Chandler  Robert G. King  Philip Jewess  Stuart E. Reynolds 《Pest management science》1991,31(3):295-304

The toxic effects of six acylurea insecticides on larvae of the tobacco hornworm were investigated at each of four environmental temperatures (20, 25, 30 and 35°C). This spans the range of temperatures which the insects can tolerate. For all the acylureas tested, mortality increased with temperature when either newly hatched or fourth-instar larvae were given insecticide in their food. Sub-lethal growth inhibition also became more pronounced at progressively higher environmental temperatures. This temperature dependence of acylurea action was not due to altered uptake of the insecticide, since there was no significant variation with temperature in the amount of [¹⁴C]flufenoxuron taken up by fifth-instar larvae when given a single meal containing labelled insecticide. Additionally, mortality of fourth-instar larvae given a single intra-haemocoelic injection of flufenoxuron was significantly greater at higher temperatures, implying that temperature affects a process that occurs after insecticide uptake. The intrinsic ability of acylureas to inhibit chitin synthesis is temperaturesensitive, since flufenoxuron inhibited the incorporation of [¹⁴C]N-acetylalucosamine into chitin by proleg epidermis in vitro significantly less well at 20°C than at the higher temperatures tested. However, there was no significant variation between the effectiveness of in-vitro chitin synthesis inhibition at 25, 30 and 35°C. These data show that the effectiveness of acylurea insecticides is subject to strong temperature effects in the range of temperatures likely to be experienced in the field.  相似文献   

Prevalence of antibodies to Leptospira serovars in sheep and goats in Alto Adige-South Tyrol     

Ciceroni L  Lombardo D  Pinto A  Ciarrocchi S  Simeoni J 《Journal of veterinary medicine. B, Infectious diseases and veterinary public health》2000,47(3):217-223

Serum samples from 313 sheep and 95 goats were collected during November 1993 in 26 localities in Alto Adige-South Tyrol and tested by microscopic agglutination test for antibodies to 28 serovars of the genus Leptospira. At the time of blood collection all the animals appeared healthy with no clinical sign suggestive of leptospirosis. The observed seroprevalence in sheep was 6.1%, whereas the seropositivity rate for goat serum samples was 2.1%. The highest serological prevalence in sheep was recorded for serovar castellonis, followed by poi, sejroe, hardjo subtype hardjobovis, copenhageni, and cynopteri. Titres to poi were the only ones found in goats. These findings, which are proof of Leptospira infection in Alto Adige-South Tyrol, indicate that foci of several serovars exist in this region.  相似文献   

Keeping up with the Joneses — Comparison of fees          下载免费PDF全文

Darren Osborne 《The Canadian veterinary journal. La revue veterinaire canadienne》2007,48(3):245-249

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Model of practice excellence — Budgeting with New Hamburg Veterinary Clinic          下载免费PDF全文

Darren Osborne  Frank Richardson 《The Canadian veterinary journal. La revue veterinaire canadienne》2006,47(9):923-928

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