全文获取类型
收费全文 | 17215篇 |
免费 | 47篇 |
专业分类
林业 | 3662篇 |
农学 | 1300篇 |
基础科学 | 142篇 |
2805篇 | |
综合类 | 805篇 |
农作物 | 2126篇 |
水产渔业 | 1818篇 |
畜牧兽医 | 1571篇 |
园艺 | 1124篇 |
植物保护 | 1909篇 |
出版年
2021年 | 9篇 |
2020年 | 17篇 |
2019年 | 22篇 |
2018年 | 2749篇 |
2017年 | 2710篇 |
2016年 | 1188篇 |
2015年 | 75篇 |
2014年 | 25篇 |
2013年 | 51篇 |
2012年 | 813篇 |
2011年 | 2178篇 |
2010年 | 2124篇 |
2009年 | 1271篇 |
2008年 | 1357篇 |
2007年 | 1605篇 |
2006年 | 66篇 |
2005年 | 123篇 |
2004年 | 136篇 |
2003年 | 194篇 |
2002年 | 95篇 |
2001年 | 25篇 |
2000年 | 56篇 |
1999年 | 19篇 |
1998年 | 9篇 |
1997年 | 15篇 |
1996年 | 15篇 |
1995年 | 11篇 |
1994年 | 6篇 |
1993年 | 22篇 |
1992年 | 22篇 |
1991年 | 14篇 |
1990年 | 14篇 |
1989年 | 14篇 |
1988年 | 20篇 |
1987年 | 11篇 |
1986年 | 11篇 |
1985年 | 9篇 |
1984年 | 9篇 |
1983年 | 5篇 |
1982年 | 7篇 |
1980年 | 7篇 |
1979年 | 12篇 |
1978年 | 9篇 |
1977年 | 9篇 |
1976年 | 5篇 |
1973年 | 5篇 |
1972年 | 5篇 |
1970年 | 6篇 |
1968年 | 9篇 |
1967年 | 7篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
1.
2.
3.
4.
5.
Outbreaks of infection due to a parapoxvirus were reported on eight New Zealand deer farms. Scabby lesions were seen variably on the muzzle, lips, face, ears and neck of red deer (Cervus elaphus) with morbidity rates reaching 100%. On three farms multifocal lesions were also present on the velvet. Deaths were reported on two properties where the lesions were extensive and secondary bacterial infections had occurred. On one of these farms multifactorial disease was suspected. Poxvirus particles were seen by negative contrast electron microscopy in scab material from all eight properties. Morphologically the deer virus resembled a parapoxvirus, but restriction endonuclease analysis showed its DNA fragment patterns were distinct from those of orf (contagious ecthyma) virus. 相似文献
6.
Latimer KS Bounous DI Colatos C Carmichael KP Howerth EW 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》1996,25(1):23-26
Extreme eosinophilia with disseminated eosinophilic granulomatous disease is described in a 4-year-old Arabian mare. Clinical signs included weight loss, coughing, jugular distention, and ventral edema. Cutaneous lesions were not observed. Eosinophilic inflammation was observed in cytologic specimens from the respiratory tract, body cavities, and lymph nodes. At necropsy, a 20-cm diameter intrathoracic mass was observed. Smaller nodules were present in the lymph nodes, liver, spleen, adrenal glands, pancreas, and skeletal muscle. Histologically, these masses and nodules were characterized by infiltrates of eosinophils, macrophages, and multinucleated giant cells, reactive fibroplasia; and multifocal eosinophilic coagula. Microscopically, mild eosinophilic infiltrates were observed in sections of stomach, small intestine, colon, and pleura; however, gross lesions were not observed in these tissues at necropsy. The etiology of the extreme eosinophilia and disseminated eosinophilic granulomatous disease in this horse was not determined. 相似文献
7.
Schneider RR Hunter DB 《The Canadian veterinary journal. La revue veterinaire canadienne》1993,34(2):103-108
A study of the pattern and relative frequency of diseases in adult female mink during the lactation period was undertaken. All adult females that died between parturition (April/May) and July 1, 1990, from 48 farms in southern Ontario were selected for study, and the cause of death was determined by gross necropsy. In addition, the cause of death was determined by gross necropsy for all adults and weaned kits that died on one farm between April 1988 and March 1989.
The mortality rate among farms in the 1990 study, for adult females during the lactation period, ranged from 0.2% to 10.1%, with a median of 1.9%. Nursing disease (56%) was the most common diagnosis, followed by mastitis (11%), metritis (8%), and dystocia (7%). Escherichia coli and Staphylococcus spp. were the most frequent isolates from the cases of mastitis. In the 1988/1989 study, the mortality rate was highest from May to July, with a large increase in June as a result of nursing disease.
相似文献8.
A method for monitoring tobacco whitefly [Bemisia tabaci (Gennadius) (Homoptera: Aleyrodidae)] populations was developed and used for timing spray applications against this pest in cotton. It was found to be successful in tests in eight commercial cotton fields. A trap composed of a yellow plastic plate (15 × 20 cm) smeared on the upper side with a thin layer of glue, was attached to an iron pole which held the plate in a horizontal position above the plants. The traps were placed 400 m apart along the periphery of the field. The sticky plates were changed weekly and the adult whiteflies were counted. When the mean weekly catch per trap reached ~ 100–200 adults, spray applications were carried out. Following this treatment,B. tabaci populations remained low and sooty mould did not develop on the lint. 相似文献
9.
10.
A. Schots J. De Boer A. Schouten J. Roosien J. F. Zil Verentant H. Pomp L. Bouwman-Smits H. Overmars F. J. Gommers B. Visser W. J. Stiekema J. Bakker 《European journal of plant pathology / European Foundation for Plant Pathology》1992,98(2):183-191
Engineering resistance against various diseases and pests is hampered by the lack of suitable genes. To overcome this problem we started a research program aimed at obtaining resistance by transfecting plants with genes encoding monoclonal antibodies against pathogen specific proteins. The idea is that monoclonal antibodies will inhibit the biological activity of molecules that are essential for the pathogenesis. Potato cyst nematodes are chosen as a model and it is thought that monoclonal antibodies are able to block the function of the saliva proteins of this parasite. These proteins are, among others, responsible for the induction of multinucleate transfer cells upon which the nematode feeds. It is well documented that the ability of antibodies to bind molecules is sufficient to inactivate the function of an antigen and in view of the potential of animals to synthesize antibodies to almost any molecular structure, this strategy should be feasible for a wide range of diseases and pests.Antibodies have several desirable features with regard to protein engineering. The antibody (IgG) is a Y-shaped molecule, in which the domains forming the tips of the arms bind to antigen and those forming the stem are responsible for triggering effector functions (Fc fragments) that eliminate the antigen from the animal. Domains carrying the antigen-binding loops (Fv and Fab fragments) can be used separately from the Fc fragments without loss of affinity. The antigen-binding domains can also be endowed with new properties by fusing them to toxins or enzymes. Antibody engineering is also facilitated by the Polymerase Chain Reaction (PCR). A systematic comparison of the nucleotide sequence of more than 100 antibodies revealed that not only the 3′-ends, but also the 5′-ends of the antibody genes are relatively conserved. We were able to design a small set of primers with restriction sites for forced cloning, which allowed the amplification of genes encoding antibodies specific for the saliva proteins ofGlobodera rostochiensis. Complete heavy and light chain genes as well as single chain Fv fragments (scFv), in which the variable parts of the light (VL) and heavy chain (VH) are linked by a peptide, will be transferred to potato plants. A major challenge will be to establish a correct expression of the antibody genes with regard to three dimensional folding, assembly and intracellular location. 相似文献