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A new diterpenoid, 15-angeloyloxy-16,17-epoxy-19-kauronic acid (1), along with five known metabolites, 16-kauren-19-oic acid (2), 6′-hydroxy-2′,3′,4,4′-tetramethoxychalcone (3), isosakuranetin (4), acacetin (5), and kaempferide (6) was isolated from the organic extracts of the roots of Chromoleana odorata. Their structures were determined by spectroscopic evidences. The structures of 1 and 2 were further confirmed by single-crystal X-ray diffraction studies. Compound 2 exhibited significant α-glucosidase inhibitory and antibacterial activities against Escherichia coli and Bacillus subtilis.  相似文献   
2.
长期以来,在生物制药行业,油佐剂疫苗的定量灌装成了令各个生产厂家头疼的问题。因疫苗中含有一定的油佐剂成分,所以黏度较大,在灌装过程中速度提高不上去,灌装量不稳定。随着GMP的进一步落实,药机行业更新换代,这些问题也逐步得到改善。罐装机械不仅从材质、结构、原理上发生改变,而且控制模式也发生较大变化。尽管如此,一些细小环节,仍对罐装速度及稳定性有一定影响。  相似文献   
3.
利用尿囊腔接种法替代绒毛尿囊膜接种法生产鸭瘟活疫苗,收获的胚液和绒毛尿囊膜及胎儿的病毒含量(ELD50)均高于<中华人民共和国兽用生物制品规程>2000年版中的病毒含量标准,而且降低了早死率,提高了鸡胚利用率.  相似文献   
4.
对于集兽用疫苗研发、生产、销售为一体的兽药企业,生产、检验用设备,仪器种类繁多,这就要求有一支与之相适应的维修管理队伍,笔者从事该行业设备维修管理工作十多年,有一些设备维修、改造方面的经历,现就这方面的经历提出几点思路,谨供同行参考。  相似文献   
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The aim of this study was to explore the feasibility of cryopreservation of inter‐subspecies cloned embryos in buffalo. In our experiment, river buffalo ear fibroblast nucleus was fused into swamp buffalo oocyte cytoplasm. The blastocyst formation rate for nuclear transfer of freshly thawed cells was not different from those of growing cells, confluent or serum‐starved cells. A total of 122 cloned blastocysts derived from cryopreserved fibroblasts were cryopreserved and thawed, 37 were survived, the cryosurvival rate was 30.3%. The survived blastocysts were transferred into 15 recipient buffalos. Five of the recipients established pregnancy, but four of them aborted on day 53, 59, 145 and 179 of gestation respectively. One cross‐bred buffalo (Murrah × Swamp buffalo (2n = 49) received three embryos delivered a 40.5 kg female calf by natural delivery on day 320 of gestation. Up to now (13‐month old), the cloned calf has been growing well with no abnormity observed. These results demonstrated that cryopreservation of inter‐subspecies cloned embryos is feasible to produce buffalo offspring.  相似文献   
6.
The essential oils of Xylopia aethiopica, Monodora myristica, Zanthoxylum xanthoxylo?des and Z. leprieurii, four Cameroonian plants used as spices in local food, showed antibacterial and antifungal activity.  相似文献   
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