Differential HSP90α expression in fish hepatocytes from polluted estuary during summer   总被引：2，自引：0，他引：2  

Ekambaram Padmini  Munuswamy Usha Rani  Bose Vijaya Geetha 《Fisheries Science》2008,74(5):1118-1126

The molecular chaperone, heat shock protein 90α (HSP90α), plays an important role in protein folding, degradation of denatured proteins and steroid activation. It is essential for the maintenance of cellular integrity and survival when induced in response to environmental, physical and chemical stresses. In the present investigation the effect of environmental stress on HSP90α expression was examined in grey mullet Mugil cephalus living in either a contaminated (Ennore) or uncontaminated (Kovalam) estuary over two seasons: Hepatocytes were isolated from grey mullet of both estuaries. Oxidative stress was determined along with HSP90α in these fish. Additionally, immunohistochemical changes were studied to confirm the HSP90α expression. Comparison of the results revealed enhanced hepatocyte oxidative stress and HSP90α expressions in fish from Ennore to a significant extent than fish from Kovalam. Also, the results showed significant seasonal variations with maximum expression observed during summer compared to the monsoon season. Overexpression of HSP90α in hepatocytes exposed to chronic environmental stress by pollutants may confer differential effects on cell survival by protecting against oxidative stress induced changes. The results also indicate that seasonal variations have significant effect on the HSP90α expression.  相似文献   

沙棘叶子提取物对白化病白鼠由铬诱导氧化胁迫的抗氧化活性评估     

郭海  赵明  S.Geetha  M.Sai Ram  S.S.Mongia  Virendra Singh  G.Ilavazhagan  R.C.Sawhney 《陕西林业科技》2006,(1):90-94

本研究报道沙棘(胡颓子科)对雄性白化病白鼠由铬诱导氧化胁迫的抗氧化活性。氧化胁迫通过对小白鼠进行强制性喂养30 d,以浓度为30 m g/kg的铬相对体重比例的重铬酸钾盐实现。铬促使体重下降,而却明显增加了器官与体重比例。铬处理明显减少了谷胱甘肽的降低,增加了丙二醛和肌氨酸磷酸激酶的水平;而且它还加强了血清中谷氨酸草酰乙酸转移酶和谷氨酸丙酮酸转移酶的浓度。用不同剂量的沙棘叶子提取物(乙醇提取)对保护铬元素诱导的氧化胁迫进行了评估,结果表明叶子提取物在浓度为100到250 m g/kg铬与体重比的情况下可以明显保护动物避免由铬所诱导的氧化伤害。  相似文献   

Experimental evaluation of Pentas lanceolata flowers for wound healing activity in rats     

Nayak BS  Vinutha B  Geetha B  Sudha B 《Fitoterapia》2005,76(7-8):671-675

The ethanolic extract of the flowers of Pentas lanceolata given by oral route to rats at dose of 150 mg kg(-1) day(-1) for 10 days, was studied for its effect on wound healing, using excision wound mode. Significant increase in granulation tissue weight, tensile strength, hydroxyproline and glycosaminoglycan content was observed. Moderate increase in protein content was seen. There was significant reduction in the wound area measurement of the test group as compared to that of controls. The efficacious prohealing action may be due to increased collagen deposition as well as better alignment and maturation.  相似文献   

Detection of OvHV-2 from an outbreak of sheep associated malignant catarrhal fever from crossbred cattle of Southern India     

N. Vinod Kumar  B. Sreedevi  A. Karthik  S. Vijaya Lakshmi  A. Geetha Reddy  D. Sreenivasulu 《Veterinary research communications》2014,38(4):323-328

An outbreak of sheep associated malignant catarrhal fever in crossbred cattle in a village of Andhra Pradesh, southern India, affected thirteen adult cows and two calves from a population of forty animals. All the affected animals were died between December and January 2013–14. The clinical and gross postmortem findings were typical of MCF in Indian crossbred cattle. Migrating sheep flocks were suspected source of infection for the cattle. The diagnosis was confirmed by heminested PCR in all the affected cattle and the suspected sheep flock. The PCR provided evidence of ovine herpes virus type 2.  相似文献   

Neoplasia in the Indian oil sardine, Sardinella longiceps (Valenciennes), and the great barracuda, Sphyraena barracuda (Edwards), from the south-east coast of India     

Gopalakrishnan A  Maran BA  Puvanendran V  Rajkumar M  Balasubramanian T  Ferguson HW 《Journal of fish diseases》2011,34(11):881-885

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Novel polyphenol molecule isolated from licorice root (Glycrrhiza glabra) induces apoptosis, G2/M cell cycle arrest, and Bcl-2 phosphorylation in tumor cell lines   总被引：4，自引：0，他引：4  

Rafi MM  Vastano BC  Zhu N  Ho CT  Ghai G  Rosen RT  Gallo MA  DiPaola RS 《Journal of agricultural and food chemistry》2002,50(4):677-684

Herbal therapies are commonly used by patients with cancer, despite little understanding about biologically active chemical derivatives. We recently demonstrated that the herbal combination PC-SPES, which contains licorice root, had anti-prostate cancer activity attributable to estrogen(s) that produced a chemical castration. A recent study also demonstrated that licorice root alone decreased circulating testosterone in men. Other studies demonstrated antitumor activity of PC-SPES in vitro associated with decreased expression of the anti-apoptotic protein Bcl-2 and in patients independent of chemical castration, suggesting that other mechanisms of antitumor activity exist separate from chemical castration. In the present study, we assessed licorice root extract for effects on Bcl-2 to identify novel cytotoxic derivatives. Licorice root extract induced Bcl-2 phosphorylation as demonstrated by immunoblot and G2/M cell cycle arrest, similarly to clinically used antimicrotubule agents such as paclitaxel. Bioassay-directed fractionations resulted in a biologically active fraction for Bcl-2 phosphorylation. HPLC separation followed by mass spectrometry and NMR identified 6 compounds. Only one molecule was responsible for Bcl-2 phosphorylation; it was identified as 1-(2,4-dihydroxyphenyl)-3-hydroxy-3-(4'-hydroxyphenyl) 1-propanone (beta-hydroxy-DHP). The effect on Bcl-2 was structure specific, because alpha-hydroxy-DHP, 1-(2,4-dihydroxyphenyl)-2-hydroxy-3-(4'-hydroxyphenyl) 1-propanone, in contrast to beta-hydroxy-DHP, was not capable of Bcl-2 phosphorylation. Pure beta-hydroxy-DHP induced Bcl-2 phosphorylation in breast and prostate tumor cells, G2/M cell cycle arrest, apoptosis demonstrated by Annexin V and TUNEL assay, decreased cell viability demonstrated by a tetrazolium (MTT) assay, and altered microtubule structure. Therefore, these data demonstrate that licorice root contains beta-hydroxy-DHP, which induced Bcl-2 phosphorylation, apoptosis, and G2/M cell cycle arrest, in breast and prostate tumor cells, similarly to the action of more complex (MW >800) antimicrotubule agents used clinically.  相似文献   

Unsaturated fatty acids from zoospores of <Emphasis Type="Italic">Sclerospora graminicola</Emphasis> induce resistance in pearl millet     

K.?N.?Amruthesh  N.?P.?Geetha  H.?J.?Lyngs J?rgensen  E.?de?Neergaard  H.?Shekar Shettyappbot@sancharnet.in" title="hss_uom@hotmail.com  appbot@sancharnet.in" itemprop="email" data-track="click" data-track-action="Email author" data-track-label="">Email author 《European journal of plant pathology / European Foundation for Plant Pathology》2005,111(2):125-137

Downy mildew of pearl millet, caused by Sclerospora graminicola, is a devastating disease, resulting in high economic losses in the semi-arid regions of the world. Recently, induction of host plant resistance using biotic and abiotic inducers are included among disease management practices as an eco-friendly approach. Unsaturated fatty acids are considered as a new generation of plant disease resistance inducers. In the present study, six unsaturated fatty acids, viz. docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA), arachidonic acid (AA), linolenic acid, linoleic acid and oleic acid, all originally detected in the zoospores of S. graminicola,were applied to seeds of susceptible cultivars of pearl millet to examine their ability to protect against downy mildew under greenhouse and field conditions. In greenhouse experiments, EPA and AA induced a maximum of 78.6% and 76.5% protection, whereas linoleic acid, DHA and linolenic acid provided up to 66.3%, 61.2% and 24.5% protection, respectively. Oleic acid was not effective in protecting pearl millet (only 5.1% protection). A time interval of four days between treatment of seeds and challenge inoculation was required to obtain optimum protection. Plants raised from treated seeds and challenge inoculated at the tillering and inflorescence stages showed enhanced resistance, resulting in higher grain yield compared to untreated plants of the same cultivar. Chitinase activity was found to be higher in susceptible seedlings of pearl millet after treatment with the fatty acids and pathogen inoculation than in seedlings only inoculated with the pathogen. This indicates that host defence responses are activated and thus that induced resistance is involved in the protection observed. The role of unsaturated fatty acids as activators of resistance against downy mildew in pearl millet is discussed.  相似文献   

Contagious caprine pleuropneumonia – a comprehensive review     

Mohd. Iqbal Yatoo  Oveas Raffiq Parray  Shah Tauseef Bashir  Muheet  Riyaz Ahmed Bhat  Arumugam Gopalakrishnan 《The Veterinary quarterly》2019,39(1):1-25

Contagious caprine pleuropneumonia (CCPP) is a serious disease of goats, occasionally sheep and wild ruminants, caused by Mycoplasma capricolum subspecies capripneumoniae (Mccp). The disease is characterized by severe serofibrinous pleuropneumonia, very high morbidity (～100%), and mortality (80–100%). CCPP affects goats in more than 40 countries of the world thereby posing a serious threat to goat farming around the globe. The characteristic clinical signs of CCPP are severe respiratory distress associated with sero-mucoid nasal discharge, coughing, dyspnea, pyrexia, pleurodynia, and general malaise. In later stages, severe lobar fibrinous pleuropneumonia, profuse fluid accumulation in pleural cavity, severe congestion of lungs and adhesion formation is observed. Mycoplasmal antigen interactions with host immune system and its role in CCPP pathogenesis are not clearly understood. CCPP is not a zoonotic disease. Diagnosis has overcome cumbersome and lengthy conventional tests involving culture, isolation, and identification by advanced serological (LAT, cELISA) or gene-based amplification of DNA (PCR, RFLP, and hybridization) and sequencing. The latex agglutination test (LAT) is rapid, simple, and better test for field and real-time diagnosis applicable to whole blood or serum and is more sensitive than the CFT and easier than the cELISA. Moreover, the studies on antibiotic sensitivity and exploration of novel antibiotics (fluoroquinolones, macrolides) can help in better therapeutic management besides preventing menace of antibiotic resistance. Re-visiting conventional prophylactic measures focussing on developing novel strain-based or recombinant vaccines using specific antigens (capsular or cellular) should be the most important strategy for controlling the disease worldwide.  相似文献   

Triterpenoidal constituents of an aqueous extract from neem kernels     

T. R. Govindachari  Geetha Gopalakrishnan  G. Suresh 《Fitoterapia》1999,70(6):318

A quantitative HPLC analysis of the major triterpenoids present in an aqueous extract of powdered neem kernels prepared in the traditional manner was undertaken. The study shows that water extracts contain all the principal triterpenoids in neem kernel though the quantities extracted are much less than in the subsequent extraction with methanol.  相似文献   

Uncommon Skin Response to the Stings of Tropical Fire Ant Solenopsis geminata     

Buddhika T.B. Wijerathne  Geetha K. Rathnayake  R.K. Sriyani Dias  Nuwan D. Wickramasinghe  Sujeewa P.B. Thalgaspitiya  Anuruddha H. Karunaratne  Suneth B. Agampodi 《The Forestry Chronicle》2018,29(4):551-554

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