Correction to: Predation on glass eels of Japanese eel <Emphasis Type="Italic">Anguilla japonica</Emphasis> in the Tone River Estuary,Japan     

Yoichi Miyake  Aigo Takeshige  Hikaru Itakura  Hajime Itoh  Hiroaki Onda  Akira Yamaguchi  Akihito Yoneta  Kohma Arai  Yulina V. Hane  Shingo Kimura 《Fisheries Science》2018,84(6):1015-1016

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Accuracy of imputation of single nucleotide polymorphism marker genotypes from low‐density panels in Japanese Black cattle          下载免费PDF全文

Shinichiro Ogawa  Hirokazu Matsuda  Yukio Taniguchi  Toshio Watanabe  Akiko Takasuga  Yoshikazu Sugimoto  Hiroaki Iwaisaki 《Animal Science Journal》2016,87(1):3-12

Using target and reference fattened steer populations, the performance of genotype imputation using lower‐density marker panels in Japanese Black cattle was evaluated. Population imputation was performed using BEAGLE software. Genotype information for approximately 40 000 single nucleotide polymorphism (SNP) markers by Illumina BovineSNP50 BeadChip was available, and imputation accuracy was assessed based on the average concordance rates of the genotypes, varying equally spaced SNP densities, and the number of individuals in the reference population. Two additional statistics were also calculated as indicators of imputation performance. The concordance rates tended to be lower for SNPs with greater minor allele frequencies, or those located near the ends of the chromosomes. Longer autosomes yielded greater imputation accuracies than shorter ones. When SNPs were selected based on linkage disequilibrium information, relative imputation accuracy was slightly improved. When 3000 and 10 000 equally spaced SNPs were used, the imputation accuracies were greater than 90% and approximately 97%, respectively. These results indicate that combining genotyping using a lower‐density SNP chip with genotype imputation based on a population of individuals genotyped using a higher‐density SNP chip is a cost‐effective and valid approach for genomic prediction.  相似文献   

Androgen induces production of male effect pheromone in female goats   总被引：1，自引：0，他引：1  

Kakuma Y  Ichimaru T  Yonezawa T  Momozawa Y  Hashizume C  Iwata E  Kikusui T  Takeuchi Y  Ohkura S  Okamura H  Mori Y 《The Journal of reproduction and development》2007,53(4):829-834

Previously we showed that the primer pheromone responsible for the "male effect" was produced in specific skin regions of castrated male goats by androgen treatments. In the present study, we examined whether androgen can also induce production of the male effect pheromone in female goats. Capsules containing dihydrotestosterone (DHT) or testosterone (T) were subcutaneously implanted into six ovariectomized (OVX) goats for 28 days. Small skin samples were collected from the head and rump regions, and the pheromone activity of their ether extracts was examined using a bioassay that monitors the electrophysiological manifestation of the hypothalamic gonadotropin-releasing hormone pulse generator as multiple-unit activity. Behaviors of OVX goats towards ovary-intact estrous goats were also examined before and at the end of DHT or T treatment. Before androgen treatment, neither the head nor rump skin samples in OVX goats showed pheromone activity. DHT treatment induced pheromone activity in the head skin sample of six OVX goats and in the rump skin sample of two OVX goats. Similar results were obtained by T treatment. In addition, OVX goats treated with T showed masculine-type sexual behaviors such as courtship and mounting behaviors towards the estrous goats. These results demonstrate that androgen is capable of inducing primer pheromone activity in the female and suggest that the synthesis pathway of the male effect pheromone exists in both sexes in the goat.  相似文献   

Vulnerability of feline T-lymphocytes to charged particles     

Kakizaki T  Hamada N  Sakashita T  Wada S  Hara T  Funayama T  Hohdatsu T  Natsuhori M  Sano T  Kobayashi Y  Ito N 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(6):605-609

An analysis of ionizing radiation-induced damage in peripheral lymphocytes has been employed to predict the prognosis of radiotherapy in terms of toxicity in normal tissues. Therefore, understanding the sensitivity of lymphocytes to high linear energy transfer (LET)-charged particles would be indispensable for utilizing charged particle therapy in veterinary medicine. However, the availability of such information is very limited. This study aimed to compare the radiosensitivity of feline T lymphocytes to gamma-rays (0.2 keV/microm) and 4 different types of charged particles with LET values ranging from 2.8 to 114 keV/microm. It was observed that the relative biological effectiveness, inactivation cross-section, and isodose-induced apoptosis increased in an LET-dependent manner. On the other hand, no difference in apoptosis frequency was observed in the cells exposed to an isosurvival dose of all the radiation types tested. This is the first study that demonstrates the LET dependence of cell killing and apoptosis induction in feline T lymphocytes. Our results suggest that lymphocytes can be effectively used to predict the prognosis of charged-particle therapy in cat patients.  相似文献   

Aggregates and gel network structure of globin hydrolysates   总被引：4，自引：0，他引：4  

Liu XQ  Ohtani T  Nakajima M  Xu QY  Ogawa Y  Sano Y 《Journal of agricultural and food chemistry》2001,49(5):2518-2522

A gel with excellent functional properties was prepared successfully using the hydrolysates of globin. In the present study, the structures of intermediate aggregates and gel network were observed directly with an electronic microscope. It was shown clearly that the intermediate aggregates were in a thin rod shape with a length of 130--140 nm, which was in good accordance with the results of the light scattering obtained in a previous study. The diameter of intermediate aggregates was 4--5 nm. Each unit of the intermediate aggregate was composed of beta-chain and peptide beta-1 in a ratio of 1:1. Its molecular weight was 26922 Da, and it had a diameter of 4.1 nm. The thin rod-shaped aggregates were formed with units through the hydrophobic interaction. The length of intermediate aggregate was >30--33 times the diameter. Furthermore, the cross-linked structure formed by peptide alpha-1 and the thin rod-shaped aggregates was also confirmed by the photography of the electronic microscope. These results supported the model proposed in previous papers as proper to depict exactly the formation and structure of the gel network of globin hydrolysates.  相似文献   

Expression and functional analysis of chemokine receptor 7 in canine lymphoma cell lines     

Toshitaka KANEI  Munetaka IWATA  Hiroaki KAMISHINA  Takuya MIZUNO  Sadatoshi MAEDA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(1):25

C-C chemokine receptor 7 (CCR7) contributes to cell homing to lymph nodes (LNs). Recent studies reported that CCR7 is also expressed in tumor cells, which correlates with LN metastasis in various cancers. However, the expression of CCR7 in tumor cells is unknown in dogs due to the lack of appropriate antibodies. In the present study, a fusion protein of C-C chemokine ligand 19 (CCL19) was employed as an alternative method to CCR7 antibodies. The fusion CCL19 protein specifically detected CCR7 expressed in canine lymphoma cell lines, which showed active chemotaxis to both canine and mouse ligands. The present study will help further research on the involvement of canine CCR7 in LN metastasis.  相似文献   

Ceramide aminoethylphosphonate from jumbo flying squid Dosidicus gigas attenuates the toxicity of cyanotoxin microcystin-LR     

Masaharu Komatsu  Naoki Ichiyama  Takashi Kurimoto  Shota Takumi  Kazuhiro Shiozaki  Yasumasa Sugiyama  Tatsuhiko Furukawa  Seiichi Ando  Saki Itonori  Hiroaki Saito 《Fisheries Science》2013,79(2):313-320

We have previously established the method for isolation of ceramide aminoethylphosphonate (CAEP) from jumbo flying squid Dosidicus gigas. In this study, we performed a MTT assay to evaluate the safety of CAEP to the cell lines for the application to health food and supplements. The CAEP did not show any cytotoxicity to various HEK293-transfectant cells. Next, we elucidated the positive function of CAEP to the somatic cells. Recently, we have reported that hepatotoxin microcystin-LR was taken up into the hepatocytes mediated by hepatocellular uptake transporters OATP1B1 and OATP1B3, and the cells were induced cytotoxicity subsequently. Cytotoxicity of microcystin-LR to permanently OATP1B3-expressing HEK293-OATP1B3 cells rather than to HEK293-OATP1B1 cells was preferentially attenuated by CAEP in a concentration-dependent manner. In addition, the enzyme activity of serine/threonine phosphatase, which was inhibited by microcystin-LR, was recuperated by co-exposure to CAEP. Furthermore, microcystin-LR-induced cellular protein phosphorylation were disrupted by CAEP exposure. These results suggested that CAEP is a promising remedy and/or preventive medicine for liver damage with microcystin-LR.  相似文献   

Effects of mangrove deforestation on fish assemblage at Pak Phanang Bay,southern Thailand   总被引：2，自引：0，他引：2  

Tatsuya Shinnaka  Mitsuhiko Sano  Kou Ikejima  Prasert Tongnunui  Masahiro Horinouchi  Hisashi Kurokura 《Fisheries Science》2007,73(4):862-870

Daytime sampling using a seine net was conducted at Pak Phanang Bay (Nakhon Si Thammarat Province, Thailand) in February and July 2006, to determine differences in fish assemblage structures between a mangrove site and an adjacent site completely cleared of mangroves. The overall numbers of fish species and individuals were significantly higher at the mangrove site than the cleared site in both months. Although benthic crustacean feeders showed more species and individual numbers at the mangrove site in both months, the opposite was found for zooplankton feeders. A cluster analysis, based on the abundance of each species, demonstrated that the fish assemblage structures were distinctly different between the two sites. In addition, significant differences in length frequency distributions for each of the four most abundant species were found between the sites in February and/or July. Small individuals of Scatophagus argus, Ambassis nalua, and Tetraodon nigroviridis were more common at the mangrove site, and of Chelon subviridis at the cleared site. These results suggest that mangrove deforestation exerts marked effects on fish assemblages.  相似文献   

Effects of dietary protein level and cold exposure on tissue responsiveness and sensitivity to insulin in sheep     

Sano H  Terashima Y 《Journal of animal physiology and animal nutrition》2001,85(11-12):349-355

The effects of dietary crude protein (CP) level and cold exposure on tissue responsiveness and sensitivity to insulin were studied in sheep. Nine rams were assigned to one of three isoenergetic diets which contained 70, 100, and 140% of CP for maintenance. They were exposed from a thermoneutral environment (20 degrees C) to a cold environment (0 degrees C) for 7 days. A hyperinsulinemic euglycemic clamp approach was applied for the determination of tissue responsiveness to insulin (the maximal glucose infusion rate, GIRmax) and tissue sensitivity to insulin (the plasma insulin concentration at half maximal glucose infusion rate, ED50). Dietary CP level influenced digestibilities of dry matter and CP (P=0.002 and P=0.001, respectively), and cold exposure decreased (P=0.01) CP digestibility. The GIRmax and ED50 tended to be influenced (P=0.08) by dietary CP level. The GIRmax was enhanced (P=0.0001) during cold exposure. Significant interactions between diet and environment were found for the GIRmax (P=0.04), but not for ED50 (P=0.07). It is concluded that in sheep dietary CP level can modify insulin action in response to cold exposure.  相似文献   

Effects of a macrolide antibiotic on enamel formation in rat incisors--primary lesion of ameloblast at the transition stage     

Abe T  Miyajima H  Okada K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(9):985-988

A novel macrolide antibiotic was administered orally to 5-week-old Jcl:Wistar rats at a dose of 5,000 mg/kg/day for 5 weeks, and then a half of animals were maintained without any treatment for 10 weeks. A white discolored lesion with horizontal stripes developed on the surface of the upper and lower incisors after dosing for 4 weeks, and these macroscopical incisal lesions disappeared with the eruption in 4 weeks after stop of administration. Histopathologically, increase in number of karyopycnosis of ameloblast at the transitional stage, vacuolar degeneration of ameloblast and cystic change in the maturation stage, and impaired iron pigment secretion at the pigmentation stage were observed. Microradiography, calcio-traumatic zones, which means hypocalcification, were observed on the superficial layer of enamel. These results suggest that the primary lesion induced by a novel macrolide antibiotic is the increased karyopycnosis of ameloblast at the transitional stage, and followed by later stage.  相似文献