Cutaneous papilloma with viral replication in an old dog.     

I Narama  K Ozaki  H Maeda  A Ohta 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1992,54(2):387-389

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Prognostic significance of surgical margin, Ki-67 and cyclin D1 protein expression in grade II canine cutaneous mast cell tumor   总被引：1，自引：0，他引：1  

Ozaki K  Yamagami T  Nomura K  Narama I 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(11):1117-1121

The prognosis for canine cutaneous mast cell tumor (CCMT) is thought to be correlated with histopathological grading. However, the wide variety of histopathologic types of grade II is one of the most troublesome and difficult points for prognosis. The objective of this study is to determine the prognostic value of surgical margin, ki-67 and cyclin D1 protein expression in grade II tumor. Surgically resected specimens of solitary grade II CCMT from 48 dogs with follow-up periods over 360 days (median was 1080 days) were used in this study. The expression of cyclin D1 and ki-67 proteins was determined by morphometrically using slides stained immunocytochemically, and the correlations among the results, survival rate, and recurrence and/or metastasis rate of each dog were analyzed statistically. The recurrence and/or metastasis and mortality rate in the incomplete surgical excision group within 30 months postoperatively were higher than that of the complete surgical excision group. In the incomplete surgical excision group, dogs with low positive staining of ki-67 had a significantly better survival, but the recurrence and metastasis rate and ki-67 positivity failed to show a significant correlation. Only a small number of cases showed cyclin D1-positive tumor cells, but most of them had a poor outcome with a high recurrence rate. In grade II CCMT, incomplete excision induced a relatively high metastasis rate and poor prognosis. Ki-67 positivity is a marker for the estimation of overall survival in incomplete surgical excision cases. Cyclin D1 positivity was low and may not have a prognostic role.  相似文献   

Multiple citrus viroids in citrus from Japan and their ability to produce exocortis-like symptoms in citron     

Ito T  Ieki H  Ozaki K  Iwanami T  Nakahara K  Hataya T  Ito T  Isaka M  Kano T 《Phytopathology》2002,92(5):542-547

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Developmental changes in pituitary–thyroid axis, and formation of gonads in leptocephali and glass eels of Anguilla spp.     

Yuichi Ozaki  Hiromi Okumura  Yukinori Kazeto  Toshitaka Ikeuchi  Shigeho Ijiri  Masaki Nagae  Shinji Adachi and  Kohei Yamauchi 《Fisheries Science》2000,66(6):1115-1122

SUMMARY: Pituitary, thyroid gland and gonads in leptocephali of Japanese eel Anguilla japonica (19.8–32.6 mm in total length), A. obscura (45.0 mm), and A. bicolor pacifica (49.5 mm) and those in glass eels of the Japanese eel were histologically and immunohistochemically examined in order to observe the developmental changes of these endocrine organs in the Anguillidae. The pituitary, consisting of adenohypophysis and neurohypophysis in Japanese eel leptocephali over 22.5 mm, did not contain thyroid stimulating hormone (TSH) immunoreactive cells. Such cells were, however, detectable in the more developed pituitaries of leptocephali of A. obscura and A. bicolor pacifica and in those of glass eels. Conversely, thyroxine (T₄)-immunoreactive thyroid follicles could be detected in all specimens, both leptocephalic and glass eel. Only in glass eels, gonads were found in the body cavity, and these gonads harbored one or two primordial germ cells (PGC) per cross-section. Our results indicate that thyroid hormones (TH) production started prior to TSH production, and that TSH and TH are both secreted during the metamorphosis from leptocephalus to glass eel. Therefore, it is plausible that the TSH–TH axis is involved in the metamorphosis from leptocephalus to glass eel, but not in the early growth from preleptocephalus to leptocephalus.  相似文献   

Isolation of Staphylococcus species from the tonsils of healthy cattle and phage patterns of isolates     

A Shimizu  J Kawano  T Fujinami  J Ozaki  S Kimura  K Sugihara 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1990,52(5):963-968

Staphylococci were found in the tonsils of 121 (75.2%) of 161 cattle. There were 15 different species, 10 belonging to novobiocin-sensitive species. The most predominant species was S. simulans (79.3% of the 121 carriers), followed by S. aureus (20.7%), S. chromogenes (10.7%) and S. epidermidis (8.3%). The other 11 species were present in 0.8 to 5.8%. Twenty-six unidentifiable isolates were isolated from 26 (21.5%) carriers. Sixty-two (51.2%) of the 121 carriers yielded two to five Staphylococcus species together while only one species could be found in each of the other 59 (48.8%). Combinations of S. simulans and other species were most frequently encountered in 50 (41.3%) of the 121 carriers. Twenty-four (96.0%) out of 25 S. aureus isolates, 3 (42.9%) of 7 S. hyicus isolates and 45 (25.4%) of 177 coagulase-negative staphylococci (13 species and unidentifiable isolates) isolates were phage typable. Most of S. aureus isolates were lysed by bovine phages 119 (n = 16) or 116 (n = 5). Thirty-three (25.4%) of 45 coagulase-negative staphylococci typable isolates with Pulverer's phage set showed the phage pattern ph5/ph9/ph10/ph12/ph13/U4/U14/U16/++ +U20/U46. The tonsils of cattle thus appear to be a suitable environment for Staphylococcus species, particularly novobiocin-sensitive species.  相似文献   

Serotypes and antimicrobial susceptibility of Pseudomonas aeruginosa strains isolated from diseased dogs   总被引：1，自引：0，他引：1  

K Ozaki  A Inoue  H Atobe  E Takahashi  S Konishi 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1990,52(2):233-239

Strains of Pseudomonas aeruginosa isolated from diseased dogs in Tokyo area during 1983 through 1986 were serotyped and assayed for antimicrobial susceptibility to obtain an epizootiological aspect of canine P. aeruginosa infection. Major sources of specimens were ear and nasal discharges and urine. The results of O-antigen typing using a monoclonal antibody kit showed that the most predominant serotype was type M. Types G and B were also major serotypes. In a yearly distribution of serotypes, type I was almost limited in 1986, and was isolated mainly from surgical wounds, which showed an episode of nosocomial infection, whereas that of type M or B was dispersed during 4 years from 1983 to 1986. As a result of antimicrobial susceptibility test, most canine strains were considered to be susceptible to 4 drugs, which were commonly used in both human and veterinary clinics, in contrast to human isolates.  相似文献   

Fine structure and differentiation of the alimentary canal in captive-bred Japanese eel Anguilla japonica preleptocephali     

Yuichi Ozaki  Hideki Tanaka  Hirohiko Kagawa  Hiromi Ohta  Shinji Adachi  Kohei Yamauchi 《Fisheries Science》2006,72(1):13-19

ABSTRACT:   The fine structure of the alimentary canal in preleptocephali produced by artificially matured Japanese eel was examined. At 1 day posthatch (dph), the alimentary canal was found only above the dorsal side of the yolk mass, and the epithelium was composed of a single layer of epithelial cells. By 5 dph, the alimentary canal was divided into three segments based on the structure of the epithelial cells: foregut, midgut and hindgut, corresponding to the future esophagus, intestine and rectum, respectively. After 7 dph, the epithelium in the foregut was surrounded by a circular muscle layer, suggesting a role in the transportation of food materials. The epithelial cells of the midgut exhibited well-developed membranous structures, which are deduced to be invaginations of the cytoplasmic membrane. Pinocytotic invaginations and vacuoles were observed in the epithelial cells of the hindgut; this observation suggests that this region is involved in the uptake of food. Significant changes in morphological features of the epithelial cells in each segment were observed until 7 dph; however, these were not evident between 7 dph and 13 dph. Consequently, the differentiation of the alimentary canal was completed by 7 dph, and preleptocephalus had developed the ability to absorb food by 7 dph.  相似文献   

Interannual variability of the ecosystem of the Kii Channel, the Inland Sea of Japan, as influenced by bottom intrusion of cold and nutrient-rich water from the Pacific Ocean, and a recent trend of warming and oligotrophication   总被引：2，自引：0，他引：2  

Koji Ozaki  Shin-Ichi Uye  Teruichi Kusumoto  Tetsuo Hagino 《Fisheries Oceanography》2004,13(1):65-79

Interannual variability of the ecosystem of the Kii Channel, productive shelf water on the Pacific side of south‐western Japan, was analysed based on physicochemical environmental variables and abundance of major zooplankton taxa collected monthly for 12 yr from 1987 to 1999. The Kii Channel experienced both short‐term (i.e. 3–4 yr) cyclical changes and a long‐term (i.e. decadal) environmental trend. The short‐term variability was primarily associated with year‐to‐year differences in intrusion of subsurface, cold nutrient‐rich water along the bottom of the Kii Channel from the Pacific Ocean. When this bottom intrusion was intense, the Kii Channel experienced a cold, new production‐dominated ecosystem. The bottom intrusion, however, has become less intense in recent years, because of the closer proximity of the Kuroshio flow axis to the Kii Channel. Hence, there was a consistent trend towards warming and a regenerated production‐dominated ecosystem. In accordance with such environmental changes, the zooplankton community shifted towards more oceanic conditions; major herbivorous calanoids (i.e. Calanus sinicus, Paracalanus parvus (s.l.), Clausocalanus spp. and Acartia omorii) decreased, while carnivores (i.e. hydromedusae and Sagitta spp.) increased. The amount of total fish catch also decreased, while the catch of subtropical species increased. These findings lead us to conclude that the bottom intrusion from the Pacific Ocean plays a key role in determining the biological production in the Kii Channel.  相似文献   

Investigation of the Heavy Metal Sources in Relation to Automobiles     

Ozaki  Hirokazu  Watanabe  Izumi  Kuno  Katsuji 《Water, air, and soil pollution》2004,157(1-4):209-223

In order to investigate the sources of roadside heavy metal pollution, auto tire, gasoline, gas oil, kerosene, paint for road markings (white, yellow and red), the soot from the discharge spout, auto tire rubber, asphalt pavement, gray paint and anticorrosive on the guardrail were gathered together and analyzed. As a consequence, Pb may be originally from yellow and red road markings, and gray paint or anticorrosive. Hg is mainly emitted from the combustion of premium gasoline and diesel soot is an important source of As, Ni and Zn. Cd and Zn are mainly emitted from the abrasion of tire rubber. The wear of asphalt pavement is considered to be a source of Ni and V.  相似文献   

旱稻水分利用率与碳同位素识别值之间的关系     

赵炳梓  张佳宝  近藤始彦  前田守弘  尾崎保夫 《土壤学报》2004,41(5):707-714

通过温室盆栽试验 ,分析和探讨了三个水平的土壤水分条件对分蘖期和成熟期收获的旱稻(OryzasativaL .)生物量累积、水分利用率 (WUE)、植株不同部位的碳同位素识别值 (CID)的影响 ,并了解了它们之间的相互关系。水分条件包括 :饱和含水量 (W1 )、饱和含水量的 70 %(W2 )、饱和含水量的 4 0 %(W3)。结果表明当土壤水分条件从W1降低到W2时 ,分蘖期收获的生物量降低 4 5 %左右 ,成熟期收获的生物量降低 1 6 %～ 1 9%;而当从W1降低到W3时 ,分蘖期收获的生物量降低 73%左右 ,成熟期收获的生物量降低 5 5 %～ 5 7%。然而 ,根据地上部干重计算而来的WUE(WUE 地上部 )和根据全株干重计算而来的WUE(WUE 全株 )则随土壤含水量的降低而增加 ,其增幅在分蘖期为 0 .0 7～ 0 .2 8gkg-1 ,在成熟期为 0 .0 7～ 0 .4 5gkg-1 。植株的CID值变幅为 1 7.0～ 2 0 .6 ,但植株不同部位间差别显著 ,分蘖期收获的样品CID值从小到大的顺序为 :根 <最近完全伸展叶 <叶芽 <茎秆 ;而成熟期收获的样品CID值从小到大的顺序为 :籽粒 <根 <茎秆 <旗叶。随着土壤含水量的降低 ,植株所有部位的CID值亦显著减小。叶部的CID值与WUE 地上部(和WUE 全株 )之间呈一致的负相关关系。  相似文献