全文获取类型
收费全文 | 705篇 |
免费 | 37篇 |
国内免费 | 2篇 |
专业分类
林业 | 31篇 |
农学 | 30篇 |
基础科学 | 1篇 |
131篇 | |
综合类 | 32篇 |
农作物 | 118篇 |
水产渔业 | 49篇 |
畜牧兽医 | 303篇 |
园艺 | 11篇 |
植物保护 | 38篇 |
出版年
2023年 | 3篇 |
2022年 | 12篇 |
2021年 | 14篇 |
2020年 | 16篇 |
2019年 | 21篇 |
2018年 | 31篇 |
2017年 | 20篇 |
2016年 | 26篇 |
2015年 | 39篇 |
2014年 | 32篇 |
2013年 | 48篇 |
2012年 | 69篇 |
2011年 | 85篇 |
2010年 | 40篇 |
2009年 | 30篇 |
2008年 | 48篇 |
2007年 | 48篇 |
2006年 | 44篇 |
2005年 | 27篇 |
2004年 | 26篇 |
2003年 | 16篇 |
2002年 | 18篇 |
2001年 | 11篇 |
2000年 | 6篇 |
1999年 | 3篇 |
1998年 | 1篇 |
1997年 | 2篇 |
1992年 | 1篇 |
1990年 | 1篇 |
1989年 | 2篇 |
1988年 | 1篇 |
1980年 | 1篇 |
1977年 | 1篇 |
1964年 | 1篇 |
排序方式: 共有744条查询结果,搜索用时 31 毫秒
1.
Effects of hyperbaric oxygen therapy on uncomplicated incisional and open wound healing in dogs
下载免费PDF全文
![点击此处可从《Veterinary surgery : VS》网站下载免费的PDF全文](/ch/ext_images/free.gif)
2.
Chan Song Kim Jin Hur Seong Kug Eo Sang-Youel Park John Hwa Lee 《Canadian journal of veterinary research》2016,80(1):40-48
Salmonella Typhimurium ghost cells expressing K88ab, K88ac, K99, and FasA fimbriae of enterotoxigenic Escherichia coli (ETEC) in their envelopes were constructed. The genes encoding the fimbriae were individually cloned into an expression plasmid, pMMP81, carrying the asd gene, which was subsequently electroporated into the Δasd S. Typhimurium mutant. Plasmid pJHLP99, carrying the phiX174 lysis gene E, was also subsequently electroporated into the Salmonella mutant. The presence of the individual fimbriae on the ghost cells was examined by Western blot analysis. Forty BALB/c mice were equally divided into 2 groups of 20 mice each. Group A mice were intramuscularly vaccinated with a mixture of the 4 ghost cells expressing the individual fimbriae. The group B mice were inoculated with sterile phosphate-buffered saline as a control. The antigen-specific serum IgG concentrations were significantly higher in group A than in group B from week 2 until week 6 after inoculation. In addition, the antigen-specific IgA concentrations in fecal samples were significantly higher in group A than in group B at week 2 after inoculation. A large difference between the groups in the number of antigen-specific IgA-secreting cells in the small intestine was observed by immunohistochemical study. Also, the splenic lymphocyte proliferative responses were significantly greater in group A than in the control mice. These results suggest that vaccination with our Salmonella ghost cells can induce both humoral and cell-mediated immune responses and that the increased number of antigen-specific IgA-secreting cells in the small intestine may be correlated with the elevated fecal IgA immune response. 相似文献
3.
Glutathione content of in vivo and in vitro matured canine oocytes collected from different reproductive stages 总被引:3,自引:0,他引:3
Kim MK Hossein MS Oh HJ Fibrianto HY Jang G Kim HJ Hong SG Park JE Kang SK Lee BC 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(6):627-632
Glutathione (GSH) concentrations of oocytes are considered as an important marker of the cytoplasmic maturation. The present study was designed to compare GSH concentrations of in vivo and in vitro matured canine oocytes. In vivo matured oocytes were collected 72 hr after ovulation by flushing fallopian tubes after laparotomy. Ovaries were collected from bitches with different reproductive stages, and collected oocytes were divided into 2 groups according to the size viz. < 120 microm and > 120 microm in diameter and cultured for 72 hr in Tissue Culture Medium-199 supplemented with 10% FBS, 2.2 mg/ml sodium bicarbonate, 2.0 microg/ml estrogen, 0.5 microg/ml FSH, 0.03 IU/ml hCG, and 1% penicillin-streptomycin solution in the presence or absence of 50 microM beta-mercaptoethanol. GSH concentrations were determined by the dithionitrobenzoic acid-glutathione disulfide (DTNB-GSSG) reductase recycling assay. GSH concentrations of immature canine oocytes were 2.9 and 3.8, 3.5 and 6.8, and 3.1 and 6.5 pM/oocyte for < 120 microm and > 120 microm in diameter oocyte groups at anestrous, follicular and luteal stage, respectively (P<0.05). In vivo matured oocytes had significantly higher GSH concentrations compared with in vitro matured oocytes. The GSH content was 19.2 pM/oocyte for in vivo matured oocytes, while 4.1 to 8.1 and 5.7 to 13.2 pM/oocyte for in vitro matured oocytes cultured in the absence or presence of beta-mercaptoethanol, respectively (P<0.05). Presence of beta-mercaptoethanol increased GSH synthesis in canine oocytes cultured in vitro, and oocytes collected from follicular and luteal stage was superior to anestrus oocytes. 相似文献
4.
Johnson Shelby L. Park Hyun Y. Vattem Dhiraj A. Grammas Paula Ma Hang Seeram Navindra P. 《Plant foods for human nutrition (Dordrecht, Netherlands)》2020,75(4):512-517
Plant Foods for Human Nutrition - Emerging data support that plant food based isoflavones have ameliorating effects on a variety of neurodegenerative diseases including Parkinson’s disease... 相似文献
5.
Eun Ju Cho Do Jin Lee Chi Do Wee Hong Lim Kim Yong Hwa Cheong Ju Sik Cho Bo Kyoon Sohn 《Scientia Horticulturae》2009,122(4):633-637
We aimed to investigate the effects of inoculating Panax ginseng C.A. Meyer seedlings with arbuscular mycorrhizal fungi (AMF) by examining the root colonization, plant nutrition uptake, growth characteristics, and soil aggregation of P. ginseng seedlings inoculated at the time of transplantation. At 16 weeks, the AMF spore density per 30 g of fresh mycorrhizosphere in seedlings inoculated with AMF (AMF+ seedlings) was 256.8 and that in seedlings not inoculated with AMF (AMF− seedlings) was 186.3, respectively. The colonization rate of AMF in the lateral roots of AMF+ seedlings was approximately 19% higher than that in the lateral roots of AMF− seedlings. The patterns of AMF colonization in ginseng roots were similar to those of the Paris-type mycorrhizal association. Plant growth characteristics, such as plant height, root length, leaf area, number of lateral roots, fresh weight of shoots and roots, and chlorophyll content, were significantly enhanced in AMF+ seedlings compared to AMF− seedlings. The macronutrient content (P, K, and Ca) and micronutrient content (Cu, Fe, and Zn) of both shoots and roots were also significantly higher in AMF+ seedlings compared to AMF− seedlings. Furthermore, glomalin content and soil aggregation were significantly enhanced in AMF inoculated areas. Our results indicate that AMF inoculation may enhance the growth of ginseng seedlings by improving the uptake of mineral nutrients and the soil structure in mycorrhizosphere. 相似文献
6.
Lee KH Cho JY Lee HJ Ma YK Kwon J Park SH Lee SH Cho JA Kim WS Park KH Moon JH 《Journal of agricultural and food chemistry》2011,59(18):10124-10128
Two novel caffeoylmalic acid methyl esters, 2-O-(trans-caffeoyl)malic acid 1-methyl ester (6) and 2-O-(trans-caffeoyl)malic acid 4-methyl ester (7), were isolated from pear (Pyrus pyrifolia Nakai cv. Chuhwangbae) fruit peels. In addition, 5 known hydroxycinnamoylmalic acids and their methyl esters were identified: 2-O-(trans-coumaroyl)malic acid (1), 2-O-(cis-coumaroyl)malic acid (2), 2-O-(cis-coumaroyl)malic acid 1-methyl ester (3), 2-O-(trans-coumaroyl)malic acid 1-methyl ester (4), and 2-O-(trans-caffeoyl)malic acid (phaselic acid, 5). The chemical structures of these compounds were determined by spectroscopic data from ESI MS and NMR. Of all the isolated compounds, five hydroxycinnamoylmalic acids and their methyl esters (2-4, 6, 7) were identified in the pear for the first time. 相似文献
7.
Kim JK Lee SY Chu SM Lim SH Suh SC Lee YT Cho HS Ha SH 《Journal of agricultural and food chemistry》2010,58(24):12804-12809
Flavonoids and carotenoids of pigmented rice ( Oryza sativa L.), including five black cultivars and two red cultivars, from Korea were characterized to determine the diversity among the phytochemicals and to analyze the relationships among their contents. Black cultivars were higher in flavonoids and carotenoids than the red and white cultivars. The profiles of eight phytochemicals identified from the rice grains were subjected to principal component analysis (PCA) to evaluate the differences among cultivars. PCA could fully distinguish between these cultivars. The Heugjinjubyeo (BR-1) and Heugseolbyeo (BR-2) cultivars were separated from the others based on flavonoid and carotenoid concentrations. Flavonoid contents had a positive correlation with carotenoid contents among all rice grains. The BR-1 and BR-2 cultivars appear to be good candidates for future breeding programs because they have simultaneously high flavonoid and carotenoid contents. 相似文献
8.
Kihoon Kim Jong Yoon Lee Hyun Kim Jaiho Shin Yongwon Shin Young Tai Yoo Hwi-Yool Kim 《Fibers and Polymers》2017,18(2):231-239
In recent decades, tremendous research has focused on the production of nanoscale fibers using synthetic polymers, with the goal of fabricating nanofibrous scaffolds for wound healing. However, the hydrophobicity of such polymers typically hinders attachment and proliferation of the cells. In this study, we combined poly-d,l-lactide-co-glycolide (PLGA) and small intestine submucosa (SIS) to fabricate blended nanofibers for wound healing by electrospinning. PLGA and SIS were dissolved in 1,1,1,3,3,3-hexafluoro isopropanol to produce different weight ratios of PLGA/SIS-blended nanofibrous membranes (NFM). Physicochemical characterization of the electrospun NFM was performed by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy, water contact angle analysis, degradation test and tensile testing. The PLGA/SIS-blended NFM showed improved hydrophilicity and tensile strength. Better infiltration, attachment and proliferation of rat granulation fibroblasts of PLGA/SIS-blended NFMs compared to PLGA NFMs were identified by morphological differences determined by SEM and a water-soluble tetrazolium salt assay kit. Based on our results, the PLGA/SIS blended NFMs were found to be suitable for use as a potential material for wound dressing. 相似文献
9.
This study was conducted to evaluate the supplementation of glutamic acid(Glu)to reduced protein diets on the performance of weanling pigs.One hundred and eighty crossbred weanling pigs([YorkshireLandrace]Duroc,21 d old)having similar body weight(BW)of 6.45 kg were randomly allotted to 1 of 6 dietary treatments(5 pigs per pen[2 barrows and 3 gilts];6 pens per treatment)based on BW and sex during a 6-week trial.Dietary treatments consisted of positive control(PC)diet formulated to have 226.9,205.6,and 188.8 g crude protein(CP)during phases 1,2,and 3,respectively,and negative control(NC)diets with 20 g CP reduction from PC diets and addition of Glu with increasing levels,resulting in the calculated Lys-to-Glu ratios of 1:2.25,1:2.30.1:2.35,1:2.40,and 1:2.45,designated as NC,NC1,NC2,NC3,and NC4,respectively.The BW of pigs receiving PC diet was higher(P<0.05)than those receiving NC diet at d 7,21 and 42.A higher(P<0.05)average daily gain(ADG)from d 1 to 7,8 to 21,22 to 42 and during the overall experiment period was observed in pigs fed PC than NC diet.Pigs fed NC diets including the graded level of Glu linearly increased(P<0.05)BW at d 42,ADG and gain-to-feed ratio(G:F)during the overall experimental period.In addition,trends in linear increase in BW(P=0.056)at d 7 and ADG from d 1 to 7 and d 22 to 42(linear effect,P=0.081,P=0.058 respectively)were observed.A tendency in the linear increment of NH3(P=0.082)at d 21 and linear reduction in methyl mercaptans(P=0.054)emission at d 42 was observed in pigs fed NC diets supplemented with graded level of Glu.In conclusion,supplementing the reduced protein diet with Glu enhanced the growth performance in weanling pigs suggesting that supplementation of Glu can compensate the reduction of 2%CP in the basal diets. 相似文献
10.
Flow cytometric assessment of ethylene glycol monoethyl ether on spermatogenesis in rats 总被引:1,自引:0,他引:1
Yoon CY Hong CM Cho YY Chung YH Min HK Yun YW Lee BJ Yang KH Lee YS Kim CK 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(2):207-212
The effects of ethylene glycol monoethyl ether (EGEE) on testicular cell populations in rats were investigated by a flow cytometric method. Rats were administered by gavage with EGEE at the various doses of 0 (saline alone), 100, 200, 400, and 800 mg/kg body weight/day for 4 weeks. The treatment of EGEE caused decreases in the weight of testis and epididymis and in the number of testicular cells. Histopathologically, exfoliation of germ cells into the tubular lumen was observed at the doses of above 200 mg/kg. The treatment of EGEE at the dose of 400 mg/kg caused moderate testicular degeneration. A significant depletion of haploid cells and a disproportionate ratio of diploid and tetraploid cells were observed as determined by flow cytometric analysis. These results indicate that the toxic effect of EGEE on the male reproductive system may be strongly associated with the disproportion of testicular germ cells. 相似文献