Genomic characterisation of bovine papillomavirus types 1 and 2 identified in equine sarcoids in Japan     

Nanako Yamashita-Kawanishi  James K. Chambers  Kazuyuki Uchida  Yumiko Tobari  Hisashi Yoshimura  Masami Yamamoto  Norio Yumoto  Hiroshi Aoki  Katsuaki Sugiura  Tohru Higuchi  Shigeaki Saito  Takeshi Haga 《Equine veterinary journal》2021,53(6):1199-1209

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Quantities and types of ceramides and their relationships to physical properties of the horn covering the claws of clinically normal cows and cows with subclinical laminitis          下载免费PDF全文

H. Higuchi  M. Nakamura  A. Kuwano  M. Kasamatsu    H. Nagahata 《Canadian journal of veterinary research》2005,69(2):155-158

Quantities and types of ceramides and their relationships to physical properties of the horn covering the claws of clinically normal cows and cows with subclinical laminitis were investigated. Total ceramide content of the horn covering the sole and wall from cows with subclinical laminitis was 872.2 +/- 146.6 microg/g and 528.6 +/- 61.3 microg/g, respectively, and was significantly (P < 0.01, 0.05) lower than that from clinically normal cows. The mean moisture content in the claws from cows with subclinical laminitis (43.5% +/- 4.3%) was significantly (P < 0.05) higher than that in the claws from clinically normal cows. The hardness of claws from cows with subclinical laminitis (35.2 +/- 3.5) was significantly (P < 0.05) less than that of claws from clinically normal cows. Significant correlations between ceramides and moisture content (P < 0.001) and between ceramide and hardness (P < 0.001) were found in clinically normal cows and cows with subclinical laminitis. Our results indicate that decreases in ceramide contents may be related to changes in physical properties of the horn covering the claw in cows with subclinical laminitis.  相似文献   

Progesterone receptor mRNA levels during pregnancy, labor, lactation and the estrous cycle in rat uterus     

Murata T  Higuchi T 《The Journal of reproduction and development》2003,49(6):425-432

Progesterone plays important roles in the regulation of female reproduction. In this study, progesterone receptor (PR) mRNA levels in rat uterus during pregnancy, labor, lactation and the estrous cycle were examined by competitive RT-PCR. During pregnancy and lactation, PR mRNA levels had decreased on day 20 of pregnancy (P20) and P21 compared with P15 but increased during labor. After a decline on day 1 of lactation (L1), PR mRNA levels had increased again on L3 and L14 compared with P15, P18, P20, P21 and P21pm (at 2200-2300 h on P21). There was no significant change in the PR mRNA level during the estrous cycle. The PR mRNA level did not change during 1 week of progesterone treatment or afterwards. Injection of 17beta-estradiol did not affect PR mRNA levels in rats treated with progesterone or those without any injections. In rats on P18, 17beta-estradiol injection did not change PR mRNA levels after sham-operation but induced an increase in PR mRNA levels of rats ovariectomized 6 h before the treatment. These results suggest that uterine PR mRNA levels are differently regulated during late pregnancy, labor and lactation, and during labor estrogen is one of the essential factors for the increase in PR mRNA levels.  相似文献   

Changes of receptor mRNAs for oxytocin and estrogen during the estrous cycle in rat uterus   总被引：1，自引：0，他引：1  

Murata T  Narita K  Honda K  Higuchi T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(6):707-712

Oxytocin receptor (OTR) mRNA levels increase dramatically near term and is potently stimulated by estrogen because increased OTR mRNA levels result from estrogen treatment in ovariectomized rat uterus. In this study, OTR, estrogen receptor (ER) alpha and ERbeta mRNA levels in the rat uterus during the estrous cycle were examined by quantitative RT-PCR. OTR mRNA levels during the estrous cycle began to increase on diestrus (P<0.05, vs value on estrus), reached maximal increase both in the morning (1000-1130 hr) and afternoon (1600-1630 hr) on proestrus (P<0.01, vs metestrus, diestrus and estrus) and then declined on estrus. In contrast ER alpha mRNA levels began to decrease on diestrus, reached statistical significance both in the morning and the afternoon on proestrus (P<0.01, vs metestrus, diestrus and estrus) and returned to the value of metestrus on estrus. ERbeta mRNA levels were low in the morning and the afternoon on proestrus (P<0.01, vs metestrus and estrus) and also returned to metestrus values on estrus. Treatments with estrogen for 3 days significantly decreased both ERalpha and ERbeta mRNA levels. It can be concluded from these results that during the estrous cycle, OTR mRNA levels in rat uterus predominantly increase at proestrus with a decrease in ERalpha and ERbeta mRNA levels, which is probably due to the increased estrogen levels in circulation before ovulation.  相似文献   

Polymerase chain reaction-restriction fragment length polymorphism analysis of the SzP gene of Streptococcus zooepidemicus isolated from the respiratory tract of horses     

Anzai T  Timoney JE  Kuwamoto Y  Wada R  Oikawa M  Higuchi T 《American journal of veterinary research》2002,63(9):1298-1301

OBJECTIVE: To develop polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis for molecular typing of strains of Streptococcus zooepidemicus and to use the new typing method to analyze a collection of isolates from the respiratory tract of Thoroughbreds. SAMPLE POPULATION: 10 strains of S zooepidemicus, 65 isolates from the respiratory tract of 9 yearlings following long distance transportation, and 89 isolates from tracheal aspirates of 20 foals with pneumonia. PROCEDURE: Phenotypic variations in the SzP protein were detected by western immunoblot analysis. Using PCR-RFLP analysis, genotypes were obtained with primer sets from the SzP gene, followed by restriction endonuclease digestion of the amplicons. RESULTS: Unique genotypic patterns were obtained with a primer set designed from both ends of the structural gene and the restriction endonuclease DdeI. Forty-five isolates from the lymphoid tissue within the pharyngeal recess (ie, pharyngeal tonsil) of yearlings included 10 SzP genotypes and SzP phenotypes. Isolates from the trachea of each yearling were of a single genotype that was also present among isolates from the pharyngeal tonsil of the same horses. Isolates from tracheal aspirates of foals belonged to 14 genotypes. CONCLUSIONS AND CLINICAL RELEVANCE: Analysis of the SzP gene by use of PCR-RFLP was effective for molecular typing of strains of S zooepidemicus in the study of respiratory tract disease in horses. Results of PCR-RFLP analysis indicate that a single strain of S zooepidemicus can migrate from the pharyngeal tonsil to the trachea at a high rate in horses undergoing long distance transportation.  相似文献   

Analysis of serum cytokine profile in a holstein heifer with leukocyte adhesion deficiency which survived for long period     

Nagahata H  Hagiwara K  Higuchi H  Kirisawa R  Iwai H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(8):683-687

Serum cytokine levels and their expression of mRNA on neutrophils from a bone marrow (BM) transplanted heifer with leukocyte adhesion deficiency were evaluated. The clinical condition of the affected heifer was relatively stable after BM-transplantation. Persistent hyper gamma-globulinemia and increased serum immunoglobulin G (IgG) concentrations were monitored longitudinally. The concentration of interleukin (IL)-1beta in serum from the affected heifer ranged from 15.8 to 321.7 ng/ml, and maximum concentration occurred at the time which coincided with peak IL-6. Serum levels of IL-6 ranged from 0.32 to 27.9 ng/m l, and they appeared to be associated with the increment of serum IgG in the affected heifer. mRNAs for IL-1beta, IL-6, IL-8 and granulocyte and macrophage colony stimulating factor (GM-CSF) were increased in neutrophils from the affected heifer compared to controls. Persistent hyper gamma-globulinemia of the affected heifer appeared to be associated with enhanced mRNA expression for IL-6 and its serum levels. These findings suggest that humoral immunity of the affected heifer is activated and the production of neutrophils appears to be enhanced under the incapability of beta(2) integrin-mediated functions of phagocytic cells.  相似文献   

Evaluation of intracranial pressure by transcranial Doppler ultrasonography in dogs with intracranial hypertension   总被引：3，自引：0，他引：3  

Fukushima U  Miyashita K  Okano S  Higuchi S  Takase K  Hagio M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2000,62(3):353-355

Transcranial Doppler ultrasonography (TCD) has been used to confirm changes in cerebral hemodynamics. In this study, we investigated whether the parameters for the basilar artery measured by TCD were correlated with the intracranial and cerebral perfusion pressures in extreme intracranial hypertension. An intracranial hypertension model was produced in seven dogs by inflating a balloon inserted into the epidural space. The resistance index was compared with the corresponding intracranial pressure and cerebral perfusion pressure values during intracranial hypertension. A significant correlation was recognized between the resistance index and cerebral perfusion pressure. Therefore, measurement of the basilar artery by TCD in the dog with intracranial hypertension is useful in estimating the intracranial circulation in cases where the measurement of intracranial pressure is not available or not indicated.  相似文献   

Activities of some enzymes of lignin formation in reaction wood of Thuja orientalis and Metasequoia glyptostroboides 2     

H. Kutsuki  T. Higuchi 《Wood Science and Technology》1982,16(4):287-291

Summary To elucidate biochemical features leading to p-hydroxyphenyl-rich lignin in gymnosperm reaction wood the activities of the following five enzymes involved in the biosynthesis of p-hydroxyphenyl lignin were compared in reaction and opposite woods: phenylalanine ammonialyase (EC 4.3.1.5), cinnamate 4-hydroxylase (EC 1.14.13.11), p-hydroxycinnamate: CoA ligase (EC 6.2.1.12), cinnamyl alcohol dehydrogenase (EC 1.1.1.-) and peroxidase (EC 1.11.1.7). The enzyme activities in the reaction woods of Thuja orientalis and Metasequoia glyptostroboides were remarkably higher than those in the opposite woods, reflecting the higher contents of p-hydroxyphenyl lignin in reaction wood.This work was partly supported by the Grant-in-Aid for Scientific Research (548047) from the Ministry of Education, Science and Culture of Japan. We thank the Ministry of Education for the support  相似文献   

Book reviews     

H. Kubler  D. Fengel  T. Higuchi 《Wood Science and Technology》1988,22(2):191-192

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A split-root apparatus for examining the effects of hydraulic lift by trees on the water status of neighbouring crops   总被引：1，自引：0，他引：1  

I. Hirota  T. Sakuratani  T. Sato  H. Higuchi  E. Nawata 《Agroforestry Systems》2004,60(2):181-187

We describe a split-root system for examining the effects of hydraulic lift by trees on crop growth. In this system, upper lateral tree roots were grown in a container set on the ground through which the taproot of the tree could penetrate into the moist soil below. The container, with a radius of 0.5 m and a height of 0.20 m, consisted of two compartments divided by a waterproof barrier. A markhamia tree (Markhamia lutea (Benth.) Schumann) and upland rice (Oryza sativa (L.)) plants were planted in one compartment, with only rice plants planted in the other compartment. Irrigation of the container was ceased at the start of the experiment. The stomatal conductance of the rice plants in the associated side, in which both trees and rice plants were grown, declined more rapidly during the first drying period than in the rice-only compartment, suggesting that there was competition for water between the tree and the crop plants. However, during the later drying period, the rice plants in the associated side were green and viable, while those in the rice-only side became desiccated. Rice roots were seen intermingling with tree roots, and the soil water content in the associated site tended to be higher than in the rice-only side. It is likely that hydraulic lift occurred in the associated side and that water that had been transferred to the surface roots was released into the soil, enabling the rice plants in this compartment to remain viable. This novel system is useful for examination of the effects of hydraulic lift by trees on the growth of neighbouring crops. This revised version was published online in June 2006 with corrections to the Cover Date.  相似文献