The influence of progesterone and prostaglandin F2α on decorin and the adhesion of caruncular epithelial cells of bovine placenta at early-mid pregnancy—Part II     

Monika Jamioł  Jacek Wawrzykowski  Marta Kankofer 《Reproduction in domestic animals》2021,56(7):1040-1049

One of the most important processes determining the proper course of gestation and its physiological termination in cows is the adhesion of epithelial cells allowing for direct contact of maternal and foetal parts of the placenta. Throughout pregnancy, placental cells are under strict hormonal control, which among others regulates the concentration and activity of specific proteins participating in the extracellular matrix remodelling of foetal membranes. The aim of the study was to evaluate the influence of progesterone and prostaglandin F_2α on the adhesion of epithelial cells at early-mid pregnancy in cows. Additionally, the impact of selected hormones on anti-adhesive properties of decorin was evaluated. Caruncular epithelial cells were isolated from healthy cows during pregnancy, immediately after slaughter. Primary cell cultures derived from the 2nd and 4th month of gestation were used in the experiments. The viability of cells was assessed by MTT assay. The adhesion of cells to fibronectin was measured spectrophotometrically. The activity of metalloproteinases was confirmed by the metalloproteinase assay. Progesterone (10^–5 and 10^–7 mol/L) and prostaglandin F_2α (10^–4_, 10^–5 and 10^–7 mol/L) increased the viability of bovine caruncular epithelial cells in the 2nd month of pregnancy. The treatment with prostaglandin F_2α significantly reduced the number of adherent cells from the 2nd month of gestation at the doses of 10^–4 and 10^–5 mol/L. Both progesterone and prostaglandin F_2α were shown to have an effect of decorin resulting in both a decrease in metalloproteinase activity and an increase in adhesion of cells to fibronectin.  相似文献   

Respiratory and hematological response of tench, Tinca tinca (L.) to a short-term cadmium exposure     

MAŁGORZATA WITESKA  BARBARA JEZIERSKA  Jacek Wolnicki 《Aquaculture International》2006,14(1-2):141-152

The effects of 3 h exposure to 96hLC50 of cadmium (4.5 mg dm⁻³) on oxygen consumption rate, and hematological parameters (RBC, WBC, erythrocyte and leukocyte pattern) of juvenile tench were evaluated. Oxygen consumption significantly decreased beginning from 24 h postexposure, and remained reduced until the end of the experiment (96 h postexposure). RBC gradually increased, together with the percentage of juvenile cells in circulation. On the other hand, cadmium induced damage to the red cells – the share of cellular anomalies significantly increased with time postexposure. They included abnormal cell shape, vacuolization, swelling, chromatin disintegration in the nucleus, and nucleus indentation. The exposed fish showed a gradual and significant decrease in WBC without a shift of lymphocyte/neutrophil proportion. No significant changes in thrombocyte count occurred. The results show that short-term exposure to cadmium reduced fish energetic metabolism, and suppressed immune abilities. The symptoms gradually developed after the end of exposure, and no recovery took place until␣96 h.  相似文献   

Response of a Juvenile Cyprinid,Lake Minnow <Emphasis Type="Italic">Eupallasella perenurus</Emphasis> (Pallas), to Different Diets     

Rafa??KamińskiEmail author  Micha??Korwin-Kossakowski  Jan?Kusznierz  Leszek?Myszkowski  L. Andrzej?Stanny  Jacek?Wolnicki 《Aquaculture International》2005,13(5):479-486

Three commercial starters (Carp Starter, Uni Starter and Perla Plus) and one non-commercial, with frozen Chironomidae larvae as a reference diet, were evaluated for the intensive rearing of juvenile lake minnow Eupallasella perenurus, a cyprinid fish that is critically endangered in Poland. The growth, condition, survival, body deformities, and chemical body composition were studied. The 90-day laboratory experiment was performed at 22 °C with fish that were initially 24.6 mm (mean total lenth (TL)) and 0.11 g (mean body weight (BW)). Satisfactory fish growth was attained with all of the diets; however, the largest (p ≤ 0.05) final size (48.5 mm TL, 1.55 g BW) and the lowest condition coefficient (K = 1.34) were noted in fish fed the non-commercial starter. The final survival rates were very high (97.5–100%). Skeletal deformities (in 74 to 92% fish) were recorded exclusively in fish fed commercial starters. All commercial starters resulted in considerably higher lipid content and lower ash content than did the non-commercial starter and the reference diet. This suggests that both these factors might be responsible for body deformities. The present results proved that only the non-commercial starter is suitable for juvenile E. perenurus rearing under controlled condition, and that none of the commercial starters can be recommended.  相似文献   

Does reduced MHC diversity decrease viability of vertebrate populations?     

Jacek Radwan  Aleksandra Biedrzycka 《Biological conservation》2010,143(3):537-544

Loss of genetic variation may render populations more vulnerable to pathogens due to inbreeding depression and depletion of variation in genes responsible for immunity against parasites. Here we review the evidence for the significance of variation in genes of the Major Histocompatibility Complex (MHC) for conservation efforts. MHC molecules present pathogen-derived antigens to the effector cells of the immune system and thus trigger the adaptive immune response. Some MHC genes are the most variable functional genes in the vertebrate genome. Their variation is clearly of adaptive significance and there is considerable evidence that its maintenance is mainly due to balancing selection imposed by pathogens. However, while the evidence for selection shaping MHC variation on the historical timescale is compelling, a correlation between levels of MHC variation and variation at neutral loci is often observed, indicating that on a shorter timescale drift also substantially affects MHC, leading to depletion of MHC diversity. The evidence that the loss of MHC variation negatively affects population survival is so far equivocal and difficult to separate from effects of general inbreeding. Some species with depleted MHC variation seem to be particularly susceptible to infection, but other species thrive and expand following severe bottlenecks that have drastically limited their MHC variation. However, while the latter demonstrate that MHC variation is not always critical for population survival, these species may in fact represent rare examples of survival despite of the loss of MHC variation. There is clearly a compelling need for data that would disclose the possible consequences of MHC diversity for population viability. In particular, we need more data on the impact of MHC allelic richness on the abundance of parasites or prevalence of disease in populations, while controlling for the role of general inbreeding. Before such evidence accumulates, captive breeding programs and other conservation measures aimed at inbreeding avoidance should be favoured over those protecting only MHC variation, especially since inbreeding avoidance programs would usually conserve both types of genetic diversity simultaneously.  相似文献   

The struggle toward a unified Europe in the area of veterinary medical education]     

E Adamczyk 《DTW. Deutsche tier?rztliche Wochenschrift》1991,98(12):458-462

The recent years have been conspicuous by an unusually dynamic arrangement of political, social and economic relations among all European countries. Scientific community of nations on our continent concerns also our discipline-veterinary medicine-giving numerous examples of common aims, beginning from the 1st Veterinary Congress in 1863, Hamburg. The present changes occurring in Europe give priority to individual scientific, professional and productive integration also in our veterinary science and practice. It must be said, too, that international exchange of goods stimulates not only their standardization as for production conditions and assessment methods, but also the level of consciousness and objective knowledge of the parties warranting the quality of the product. This concerns particularly the food products as for which the parties interested take into account the technical and sanitary norms concerning the products at all stages of their production. Hence the tendency to unification of veterinary studies in all Europe has been approved by all four facilities in Poland. Accession of our faculties to the European Association of Veterinary Medicine Faculties direct by Prof. TOMA, as well as of Czechoslovak and Hungarian faculties, is a clear example of these countries being ready to integration. Similarly, the resolutions of the Association concerning standardization of diplomas and professional specializations of veterinary surgeons in European Community would inspire the veterinary education centres to comparing and following unified organization and programmes of studies.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

Physiological and biochemical characterization of quinclorac resistance in a false cleavers (Galium spurium L.) biotype     

Van Eerd LL  Stephenson GR  Kwiatkowski J  Grossmann K  Hall JC 《Journal of agricultural and food chemistry》2005,53(4):1144-1151

The physiological and biochemical basis for quinclorac resistance in a false cleavers (Galium spurium L.) biotype was investigated. There was no difference between herbicide resistant (R) and susceptible (S) false cleavers biotypes in response to 2,4-D, clopyralid, glyphosate, glufosinate-ammonium, or bentazon. On the basis of GR(50) (growth reduction of 50%) or LD(50) (lethal dose to 50% of tested plants) values, the R biotype was highly resistant to the acetolactate synthase (ALS) inhibitor, thifensulfuron-methyl (GR(50) resistance ratio R/S = 57), and quinolinecarboxylic acids (quinclorac R/S = 46), resistant to MCPA (R/S = 12), and moderately resistant to the auxinic herbicides picloram (R/S = 3), dicamba (R/S = 3), fluroxypyr (R/S = 3), and triclopyr (R/S = 2). The mechanism of quinclorac resistance was not due to differences in [(14)C]quinclorac absorption, translocation, root exudation, or metabolism. Seventy-two hours after root application of quinclorac, ethylene increased ca. 3-fold in S but not R plants when compared to controls, while ABA increased ca. 14-fold in S as opposed to ca. 3-fold in R plants suggesting an alteration in the auxin signal transduction pathway, or altered target site causes resistance in false cleavers. The R false cleavers biotype may be an excellent model system to further examine the auxin signal transduction pathway and the mechanism of quinclorac and auxinic herbicide action.  相似文献   

Influence of Agricultural Land Use and Management on the Contents of Polycyclic Aromatic Hydrocarbons in Selected Silty Soils     

Patryk Oleszczuk  Jacek Pranagal 《Water, air, and soil pollution》2007,178(1-4):195-205

The characteristics of transport and transformation of SO₂, NO_x and O₃ in northeast Asia have been investigated by using a comprehensive regional air quality model (RAQM) driven by a meteorological model MM5. A study period of 1-15 March 2002 has been selected due to the availability of intense observation of chemical species for both ground and upper levels. Model results have been compared against observational data to provide insights into the strength and weakness of the model’s ability and the evolutionary features of chemical species. Validation shows a good skill of this model system in reproducing most of the key features in long-range transport, but apparent bias still remains due to a series of uncertainties from either emission estimates, prescribed parameters, or inherent model limitations. In general, this model shows a better skill for SO₂ and O₃ than for NO_x. Large discrepancy occurs between the observed and calculated NO_x concentration at higher levels, with the model results being much lower. A series of sensitivity tests have been conducted to investigate the potential affecting factors and it is found that the inaccuracy or incompleteness in currently used emission inventories could be a most likely cause for such discrepancy. Long-range transport from Asian continent to the western Pacific is pronounced in springtime. Substantially high concentrations of SO₂ and NO_x in or above the boundary layer (0.5～3.0 km) over the Yellow Sea suggest an important pathway for long-range transport in northeast Asia.  相似文献   

Use of Enrichment,and Contamination Factors Together with Geoaccumulation Indexes to Evaluate the Content of Cd,Cu, and Ni in the Rybnik Water Reservoir in Poland   总被引：1，自引：0，他引：1  

Loska  Krzysztof  Cebula  Jan  Pelczar  Jacek  Wiechuła  Danuta  Kwapuliński  Jerzy 《Water, air, and soil pollution》1997,93(1-4):347-365

In this paper, the contamination degree of the Rybnik Reservoir with cadmium, copper and nickel was analyzed. Quality of the water from the reservoir was determined by drawing comparisons between the metal content in the water and both the officially permitted levels (contamination factor) and levels of metals occurring in the water of non-contaminated areas (enrichment factor). Contamination of bottom sediment with chosen metals was analyzed with reference to the metal content in mudstone (geoaccumulation index, enrichment factor, contamination factor). Trends towards changing the metal content in the bottom sediment was analyzed by determining the enrichment factor of the surface layer of the bottom sediments in relation to a deeper layer. Enrichment of the bottom sediments with metals coming from the water was also determined.  相似文献   

The impact of a bio-fertilizer on the soil organic matter status and carbon sequestration—results from a field-scale study     

Bożena Dębska  Jacek Długosz  Anna Piotrowska-Długosz  Magdalena Banach-Szott 《Journal of Soils and Sediments》2016,16(10):2335-2343

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Exogenous leptin advances puberty in domestic hen     

Paczoska-Eliasiewicz HE  Proszkowiec-Weglarz M  Proudman J  Jacek T  Mika M  Sechman A  Rzasa J  Gertler A 《Domestic animal endocrinology》2006,31(3):211-226

The present study was undertaken to examine the effect of recombinant chicken leptin administered to fed ad libitum and feed-restricted immature chickens of a layer strain on ovarian development and the timing of sexual maturity. In the first experiment 11-week-old pullets (77 days of age) fed ad libitum were injected daily with leptin at four dose levels (4, 16, 64 and 256 microg/kg body weight) until sexual maturity (lay of the first egg). Leptin treatment at the highest dose significantly (P<0.05) advanced the onset of puberty (day 116.3+/-1.0) in comparison to controls (day 121.3+/-1.2). The rises of luteinizing hormone, estradiol and progesterone in blood plasma were also advanced by leptin treatment. In the second experiment, both full-fed and feed-restricted pullets (79 days of age) were injected daily with leptin (256 microg/kg body weight). In birds fed ad libitum, exogenous leptin again significantly (P<0.05) advanced first ovipostion (day 118.4+/-1.4 versus day 124.4+/-1.7), while abolishing the significant (P<0.05) delay caused by feed restriction (day 131.5+/-1.6) and restoring the normal onset of sexual maturity (day 125.7+/-1.6). Analysis of the ovaries in 106-day-old pullets revealed that leptin injections advanced follicular development, particularly in birds fed ad libitum, and significantly (P<0.01) reduced follicular apoptosis both in full-fed and feed-restricted birds. In conclusion, we have shown that in female chickens exogenous leptin advances the onset of puberty by attenuation of ovarian apoptosis and enhancement of folliculogenesis.  相似文献