排序方式： 共有22条查询结果，搜索用时 234 毫秒
1.
Prevalence of Cryptosporidium infection in goats in selected locations in three agroclimatic zones of Sri Lanka 总被引：1，自引：0，他引：1
Noordeen F Rajapakse RP Faizal AC Horadagoda NU Arulkanthan A 《Veterinary parasitology》2000,93(2):95-101
The prevalence of Cryptosporidium oocysts in the faeces of 1020 goats in three age categories was examined during 1999 in selected locations of three agroclimatic zones of Sri Lanka. The oocysts were demonstrated using the Sheather's sucrose flotation method followed by staining with the modified Ziehl Neelsen technique. Cryptosporidium oocysts were detected in animals from all agroclimatic zones with the highest prevalence of infection in the dry zone (33.6%) compared with 24.7 and 21.7% in the intermediate zones and wet, respectively (P<0.001). Overall, Cryptosporidium oocyst counts were significantly higher in goats of <6 months and 7-12 months of age groups compared with goats of >12 months of age (P<0.001). Cryptosporidium oocysts were detected in 291/1020 (28.5%) animals, while 194/1020 animals (19%), 84/1020 animals (8.2%) and 13/1020 animals (1.3%) excreted low (1-1000 oocysts per gram of faeces), moderate (1000-5000 oocysts per gram of faeces) and high (>5000 oocysts per gram of faeces) counts, respectively. The mean Cryptosporidium count was 383 oocysts per gram of faeces. The majority of the infected goats were asymptomatic. These animals are likely to play an important role in the epidemiology of cryptosporidiosis in goat kids and humans. 相似文献
2.
Antioxidant properties of a radical-scavenging peptide purified from enzymatically prepared fish skin gelatin hydrolysate 总被引：8，自引：0，他引：8
Hoki (Johnius belengerii) skin gelatin was hydrolyzed with three commercial enzymes to identify radical-scavenging potencies of derived peptides. Peptides derived from tryptic hydrolysate exhibited the highest scavenging activities on superoxide, carbon-centered 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals assessed by ESR spectroscopy. Following consecutive chromatographic separations of tryptic hydroolysate, the peptide sequence His-Gly-Pro-Leu-Gly-Pro-Leu (797 Da) acted as a strong radical scavenger under studied conditions. Further, this peptide could act as an antioxidant against linoleic acid peroxidation and the activity was closer to the highly active synthetic antioxidant butylated hydroxytoluene (BHT). In addition, antioxidative enzyme levels in cultured human hepatoma cells were increased in the presence of this peptide and it was presumed to be the peptide involved in maintaining the redox balance in the cell environment. Present data indicate that free-radical-scavenging activities of hoki skin gelatin peptides substantially contribute to their antioxidant properties measured in different oxidative systems. 相似文献
3.
The protozoan parasite N. caninum is a major pathogen in cattle and dogs. However, clinical symptoms are occasionally described for other potential hosts. Natural abortion in goats due to N. caninum has been rarely reported and only little data is available on the seroprevalence of N. caninum in this species. In the present study, 486 goats from Sri Lanka were tested in a crude antigen ELISA for the presence of serum antibodies against N. caninum. Additionally, the sera were analysed by N. caninum-Western blot and indirect fluorescence antibody test (IFAT). In all three tests applied, only three sera (0.7%) were scored clearly positive for anti-N. caninum antibodies. The optimal correlation between ELISA, IFAT, and Western blot confirms the suitability of the ELISA for large-scale seroepidemiologic studies, not only in cattle but also in goats. 相似文献
4.
Kalubowila DG Udagama-Randeniya PV Perera NA Rajapakse RP 《Journal of veterinary medicine. B, Infectious diseases and veterinary public health》2004,51(2):89-93
An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of antibodies to Sarcosystis infection in cattle and buffaloes. Crude antigens derived from cystozoites of Sarcosystis cruzi and antibodies from the sera of naturally infected cattle and buffaloes were used. The assay was validated by determination of the specificity, sensitivity and negative and positive predictive values. Using ELISA, a sero-epidemiological survey for sarcosystosis was carried out in cattle (n = 215) and buffaloes (n = 123) from the wet and dry zones of Sri Lanka. The prevalence of infection in cattle was 69.3%. Prevalence of the infection collectively in both cattle and buffaloes in the dry zone was significantly higher (P < 0.001) than that in the wet zone. The number of sero-positive cattle and buffaloes in the dry zone was significantly higher than that in the wet zone (P < 0.05). Buffaloes in the dry and wet zones showed a greater prevalence of infection (P < 0.05) than cattle. 相似文献
5.
Dangolla A Ekanayake DK Rajapakse RP Dubey JP Silva ID 《Veterinary parasitology》2006,137(1-2):172-174
Serum samples collected during August 2003-June 2004 from 45 privately owned captive and 8 elephants from the Pinnawala Elephant Orphanage were tested for the presence of antibodies against Toxoplasma gondii using the direct modified agglutination test (MAT). Antibodies were found in sera of 14 of 45 (32%) privately owned elephants with titers of 1:25 in three, 1:50 in three, 1:100 in three, 1:200 in three, and 1:400 in three elephants. The elephants from Pinnawala Elephant Orphanage were seronegative. This is the first report of T. gondii seroprevalence in elephants in Sri Lanka. 相似文献
6.
Summary Protein banding patterns of 119 lines (85 Japanese potato lines and introductions, 22 dihaploids, 1 wild source (Solanum bulbocastanum Dun.), 10 transformants and 1 somaclonal variant) were compared by sodium dodecylsulfate polyacrylamide gel electrophoresis
(SDS-PAGE). Microtubers formed in vitro were used as the source of plant materials. There were more than 15 bands in the 35–20
kDa molecular weight range and less than 10 bands in the 65–40 kDa range. The bands in the 65–40 kDa range which corresponded
to the potato tuber storage protein patatin were focussed to about 40 kDa after heat treatment. The results indicate a distinct
difference in the banding patterns among potato lines. 相似文献
7.
The effect of methyl jasmonate (MeJA) in terms of its induction of inherent bioactive chemicals in sweet basil (Ocimum basilicum L.) was evaluated after MeJA was sprayed on healthy basil plants. The total phenolic content of the sweet basil significantly increased after 0.1 and 0.5 mM MeJA treatments compared with the control not subjected to MeJA. Two phenolic compounds, rosmarinic acid (RA) and caffeic acid (CA), were identified as strong antioxidant constituents of the sweet basil. Their amounts also significantly increased after the MeJA treatment. In addition, eugenol and linalool increased 56 and 43%, respectively, by the 0.5 mM MeJA treatment. Due to the accumulation of RA, CA, and eugenol, which possess strong 2,2-diphenyl-1-picrylhydrazyl (DPPH*) free radical scavenging activities, the antioxidant activity of the sweet basil extract was 2.3-fold greater than that of the control after the 0.5 mM MeJA treatment. In the DPPH* assay, the EC50 values of RA, CA, and eugenol were determined as 23, 46, and 59 microM, respectively, which indicated they were 6-, 3-, and 2.4-fold more efficient than BHT (140 microM). Besides, an unidentified HPLC peak in the methanolic extract of the sweet basil was 4.3-fold higher than that of the control after the 0.5 mM MeJA treatment. 相似文献
8.
9.
F Noordeen N U Horadagoda A C M Faizal R P V J Rajapakse M A A Razak A Arulkanthan 《Veterinary parasitology》2002,103(3):217-225
An experimental study was carried out in neonatal goat kids to examine the infectivity of Cryptosporidium oocysts, pattern of oocyst shedding and morphological changes in the intestine during the infection. Cryptosporidium oocysts isolated from adult asymptomatic goats, and identified as C. parvum by polymerase chain reaction (PCR) were used in this study. Of three 4-day-old goat kids, two were orally infected with C. parvum oocysts (10(5) oocysts in 10 ml PBS/kid). One goat kid given 10 ml PBS only by the oral route served as a control. Cryptosporidium oocysts were detected in the faeces of one infected kid on day 3 post-inoculation (pi) whereas in the other 6 days pi. The faecal oocyst counts gradually increased and the peak counts in the two kids were 2 x 10(6)g(-1) (on day 12 pi) and 3.2 x 10(6)g(-1) (on day 14 pi). The increase in faecal oocyst output coincided with diarrhoea in an infected kid from days 10-17 pi. Although the oocyst excretion declined gradually after the peak, both infected kids excreted oocysts until euthanized on days 20 and 22 pi. Light and scanning electron microscopic investigations of the ileum revealed the endogenous stages on the brush border of the enterocytes, infiltration of neutrophils and mononuclear cells into the lamina propria, atrophy, stunting and fusion of villi. For purposes of comparison, goat Cryptosporidium oocysts were inoculated orally (10(3) oocysts/mouse) to eight, 1-week-old mice. All experimental mice excreted oocysts from day 3 pi, and four infected mice continued to excrete oocysts up to day 42 pi. The experimental infection described in goat kids resembled the natural disease in terms of oocyst excretion, clinical signs and intestinal pathology. The ability of oocysts excreted by asymptomatic goats, to infect goat kids and mice is likely to have a major impact on the epidemiology of cryptosporidiosis in livestock and man. 相似文献
10.
Faizal AC Rajapaksha WR Rajapakse RP 《Journal of veterinary medicine. B, Infectious diseases and veterinary public health》2002,49(3):115-119
The benefit of gastrointestinal nematode control in cross-bred goats in the dry area of Sri Lanka was studied throughout 1 year by comparing the performances of three groups: group 1 animals (n = 11) were left untreated against gastrointestinal nematodes; group 2 animals (n = 11) were given monthly anthelmintic treatment. doramectin, at the dose rate of 300 microg/kg bodyweight during the entire study period; group 3 animals (n = 11) were also given monthly doramectin treatment at the dose rate of 300 microg/kg bodyweight but only during the rainy months, October to January and again once in April. All three groups were turned out together on to communal pasture. Tracers were used for the monthly estimation of gastrointestinal nematode burdens. Four untreated animals and a group 3 animal suffered from parasitic gastroenteritis during the study period. When compared with the controls, the treated groups had significantly lower gastrointestinal nematode infection as indicated by faecal egg counts (P<0.01). Faecal egg counts in group 3 were significantly higher than those in group 2 (P < 0.01). When compared with the controls, the treated groups had significantly greater weight gains (P< 0.05). Haemonchus contortus and Trichostrongylus colubriformis were the predominant gastrointestinal nematodes identified from tracers. The worm burden varied according to the rainfall pattern, with very minimal transmission during the dry period which extends from May to August. Under the conditions of this study, the monthly anthelmintic treatments increased weight gains and prevented parasitic gastroenteritis of goats in the dry zone of Sri Lanka. 相似文献