Hemodynamic effects of incremental doses of acepromazine in isoflurane-anesthetized dogs     

Julia P.P. Rangel  Eduardo R. Monteiro  Flavia S. Bitti  Juarez S.N. Junior  Daniela Campagnol 《Veterinary anaesthesia and analgesia》2021,48(2):167-173

ObjectiveTo evaluate the effects of incremental doses of acepromazine on hemodynamics in isoflurane-anesthetized dogs.Study designProspective, experimental study.AnimalsHealthy, adult, mixed-breed dogs (two male and four female) weighing 16.8 ± 5.1 kg (mean ± standard deviation).MethodsDogs were anesthetized with propofol (7 mg kg^–1) intravenously (IV) and isoflurane. Thermodilution and arterial catheters were placed for hemodynamic monitoring and arterial blood sampling for blood gas analysis. Baseline measurements were performed with stable expired concentration of isoflurane (Fe′Iso) at 1.8%. Each dog was then administered four incremental acepromazine injections (10, 15, 25 and 50 μg kg^–1) IV, and measurements were repeated 20 minutes after each acepromazine injection with Fe′Iso decreased to 1.2%. The four acepromazine injections resulted in cumulative doses of 10, 25, 50 and 100 μg kg^–1 (time points ACP₁₀, ACP₂₅, ACP₅₀ and ACP₁₀₀, respectively).ResultsCompared with baseline, cardiac index (CI) increased significantly by 34%, whereas systemic vascular resistance index (SVRI) decreased by 25% at ACP₅₀ and ACP₁₀₀. Arterial oxygen content (CaO₂) was significantly lower than baseline after all acepromazine injections (maximum decreases of 11%) and was lower at ACP₅₀ and ACP₁₀₀ than at ACP₁₀. No significant change was found in heart rate, stroke index, oxygen delivery index and systolic, mean and diastolic blood pressures. Hypotension (mean arterial pressure < 60 mmHg) was observed in one dog at baseline, ACP₁₀, ACP₂₅ and ACP₁₀₀, and in two dogs at ACP₅₀.Conclusions and clinical relevanceCompared with isoflurane alone, anesthesia with acepromazine–isoflurane resulted in increased CI and decreased SVRI and CaO₂ values. These effects were dose-related, being more pronounced at ACP₅₀ and ACP₁₀₀. Under the conditions of this study, acepromazine administration did not change blood pressure.  相似文献   

Polymethylmethacrylate orbital implants with interconnecting channels. A retrospective study following enucleation in dogs and cats          下载免费PDF全文

Arianne P. Oriá  Mary'Anne Rodrigues de Souza  Francisco de Assis Dórea Neto  Deusdete C. Gomes Junior  Alessandra Estrela‐Lima  Caterina Muramoto  João Moreira Costa Neto  Ron Ofri 《Veterinary ophthalmology》2016,19(2):102-109

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Pronuclear Embryo Yield in Canindé and Saanen Goats for DNA Microinjection     

RR Moura  ES Lopes‐Junior  DIA Teixeira  IA Serova  LE Andreeva  LM Melo  VJF Freitas 《Reproduction in domestic animals》2010,45(5):e101-e106

The objective of this study was to examine the effect of donor breed on pronuclear‐stage embryo yield to be used for DNA microinjection in a transgenesis goat program. Twelve Canindé and twelve Saanen goats were heat synchronized using a progestagen‐cloprostenol treatment. Forty‐eight hours before the sponge removal, superovulation was induced with a total administration of 4.4 mg/kg bodyweight NIH‐FSH‐P1, given twice daily in decreasing doses over 3 days. In addition, goats received 100 μg of GnRH and they were hand‐mated at 36 and 48 h after progestagen removal. Embryo recovery was performed by oviduct flushing at 72 h after sponge removal. Embryos were microinjected with a DNA construct and noticeable swelling of the nuclei was the criterion for successful microinjection. The total diameter, cytoplasm diameter, zona pellucida thickness and pronuclei diameter were measured for each microinjected embryo. A higher (p < 0.05) percentage of fertilized ova was observed in Canindé (89.9%) than Saanen (36.2%) goats. In addition, Canindé donors produced a higher percentage of pronuclear embryos when compared with Saanen: 72.5% vs 20.6% (p < 0.05), respectively. Successful microinjection was verified in 96.7% and 73.3% of times in Canindé and Saanen embryos, respectively (p < 0.05). Significant differences were observed for all morphometric parameters except for cytoplasm diameter. In conclusion, under our study experimental conditions, Canindé were more efficient than Saanen goats concerning the pronuclear embryo yield and manipulation. The use of Canindé goats in transgenesis programs could be increase the interest in their breeding and could be contribute to saving them from extinction.  相似文献   

Empirical models to predict LAI and aboveground biomass of Coffea arabica under full sun and shaded plantation: a case study of South of Minas Gerais,Brazil     

Priscila Pereira Coltri  Jurandir Zullo Junior  Vincent Dubreuil  Glaucia Miranda Ramirez  Hilton Silveira Pinto  Gustavo Coral  Camila Giorgi Lazarim 《Agroforestry Systems》2015,89(4):621-636

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Immunohistochemical Expression of Growth Factors in the Follicular Wall of Normal and Cystic Ovaries of Sows          下载免费PDF全文

FJF Sant'Ana  JL Reis Junior  GR Blume  EJ Gimeno  F Rey  HH Ortega 《Reproduction in domestic animals》2015,50(2):327-332

The expression of growth factors was evaluated immunohistochemically in normal and cystic ovaries of sows. The immunohistochemically stained area (IHCSA) was quantified by image analysis to analyse the expression of these proteins in the follicular wall of secondary, tertiary and cystic follicles. IGF‐I immunoreactivity was strong in the granulosa cell layer (GC), moderate in the theca interna (TI) and mild in the theca externa (TE) of the normal follicles. There was severe reduction of the labelling to IGF‐I in the GC of the follicular and luteinized cysts. In the normal follicles, the reactivity for IGF‐II was very similar to pattern noted in IGF‐I. There was reduction of the IHCSAs in the GC of the follicular and luteinized cysts, but the decrease was not significant. The staining of the IGF‐II in the TI and TE of the cysts was increased, in comparison with normal follicles. The IHCSAs for VEGF were higher in the GC and TE of the normal follicles in contrast to TI, but this difference was noted only in the tertiary follicle. The VEGF reactivity increased in the GC of the cysts, in relation to normal follicles. The results of the current study show that the formation of ovarian cysts in sows is associated with alterations in the immunohistochemical expression of some growth factors.  相似文献   

Effect of Relaxin on Fertility Parameters of Frozen–Thawed Buffalo (Bubalus bubalis) Sperm          下载免费PDF全文

AR Elkhawagah  V Longobardi  G Neglia  A Salzano  G Zullo  GA Sosa  G Campanile  B Gasparrini 《Reproduction in domestic animals》2015,50(5):756-762

The aim of this work was to evaluate the effect of relaxin on fertility parameters of buffalo frozen/thawed sperm. Sperm were incubated in the absence of capacitating agents (negative control), with a known capacitating agent such as heparin (positive control) and with 50 and 100 ng/ml relaxin for 2 and 4 h. Sperm viability, motility, capacitation and the effect of relaxin on the fertilizing ability after heterologous IVF were evaluated. Although viability was not affected, relaxin increased (p < 0.05) sperm motility compared to the negative and positive controls both after 2 h (60.0 ± 2.0, 60.0 ± 3.1, 68.3 ± 1.7 and 69.4 ± 2.7, respectively, in negative control, positive control, 50 and 100 ng/ml relaxin) and 4 h (55.0 ± 2.5, 53.3 ± 3.0, 62.2 ± 3.0 and 65.0 ± 3.2, respectively, in negative control, positive control, 50 and 100 ng/ml relaxin) incubation. When sperm were incubated with both 100 ng/ml relaxin and heparin, a decrease (p < 0.01) of pattern A, that is low capacitation level, was observed compared to the negative control both after 2 h (54.4, 34.3 and 36.4%, respectively, in negative control, positive control and 100 ng/ml relaxin) and 4 h (51.9, 35.0 and 34.3%, respectively, in negative control, positive control and 100 ng/ml relaxin). Moreover, an increase (p < 0.01) of pattern EA, that is high capacitation level, was recorded with 100 ng/ml relaxin and heparin compared to the negative control both after 2 h (44.1, 59.3 and 57.7%, respectively, in negative control, positive control and 100 ng/ml relaxin) and after 4 h (43.0, 54.4 and 56.0%, respectively, in negative control, positive control and 100 ng/ml relaxin). Finally, relaxin increased (p < 0.01) cleavage rate compared to the negative control (57.1 ± 4.4, 72.5 ± 6.0, 71.4 ± 5.5 and 73.6 ± 2.9, respectively, in negative control, positive control, 50 and 100 ng/ml relaxin). In conclusion, relaxin has a beneficial effect on motility, capacitation and fertilizing ability of frozen–thawed buffalo sperm.  相似文献   

Potential distribution and population trends of the smalltail shark Carcharhinus porosus inferred from species distribution models and historical catch data     

Leonardo Manir Feitosa  Lucas Pereira Martins  Leandro Augusto de Souza Junior  Rosangela Paula Lessa 《水产资源保护:海洋与淡水生态系统》2020,30(5):882-891

1. Updated distribution ranges are crucial for conservation status assessments. Comprehensive analyses combining published literature and available data on historical catches and species distribution models (SDMs) are effective tools that could improve the prediction of more realistic scenarios for some species, especially those with limited information available and facing multiple threats.
2. The present study aimed at generating an updated distribution for the smalltail shark Carcharhinus porosus, one of the most threatened and understudied shark species of the western Atlantic Ocean. Estimates of the key areas for this species conservation based on the SDMs, and trends in catch probabilities throughout its distribution range are provided.
3. Four algorithms (BIOCLIM, Domain, Mahalanobis, and Maximum Entropy) were used to model the distribution of C. porosus and calculate its habitat suitability based on marine environmental variables. To assess historical catch probability trends, we built a generalized linear model from published and grey literature data. This analysis was used to estimate catch probability as an indication of population trends.
4. SDMs suggest that the northern coast of South America (NCSA) harbours the most suitable habitats for C. porosus in the world, which was expected given its historically high catch rate in this region. In addition, there was a continuously declining catch probability trend starting in the 1970s. However, the decline was smaller for the NCSA as compared with the Gulf of Mexico and the eastern South America coast.
5. Results indicate that the NCSA should be considered the currently most important area in the world for this species conservation. Furthermore, the lack of data throughout Central and South American marine regions hampers the evaluation of extinction risk throughout its updated distribution. Thus, research in these areas is urgently required for a more comprehensive conservation status assessment.

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Performance of four <Emphasis Type="Italic">Trichogramma</Emphasis> species (Hymenoptera: Trichogrammatidae) as biocontrol agents of <Emphasis Type="Italic">Heliothis virescens</Emphasis> (Lepidoptera: Noctuidae) under various temperature regimes     

Gilberto Santos Andrade  Dirceu Pratissoli  Leandro Pin Dalvi  Nicolas Desneux  Hugo José Gonçalves dos Santos Junior 《Journal of pest science》2011,84(3):313-320

Trichogramma spp. are major parasitoids of lepidopteran pest eggs, but there is large variation in efficacy toward a given pest among the numerous described Trichogramma species. It is important to select the Trichogramma species that most effectively parasitize and develop in target pest eggs for biological control. In this context, Trichogramma pretiosum, T. exiguum, T. atopovirilia and T. acacioi were studied in Heliothis virescens eggs under different thermal conditions. The parasitoids were reared at constant temperatures of 20, 25 and 30°C and tested at these respective temperatures, while parasitoids reared at 25°C were also tested at 20 and 30°C, for a total of 20 species–temperature combinations. About 30 H. virescens eggs were offered to the parasitoids for 24 h. Among the four species, parasitism rate by T. atopovirilia was highest at all temperature conditions, whereas T. acacioi had the lowest rates of parasitism at 25°C and 25/30°C. Parasitism ranged from 13.8 to 43.8% among all species–temperature combinations. Viability (emerged parasitoids) ranged from 80.8 to 98.4%, and was deemed satisfactory. The emergence rates of T. exiguum and T. acacioi were affected by temperature. Temperature also affected the sex ratio of T. exiguum at 25/30°C, whereas T. pretiosum and T. acacioi produced females predominantly independent of temperature. Overall, the parasitoid T. atopovirilia was the most efficient in parasitizing H. virescens eggs, though the levels of parasitism obtained might not ensure its successful use in biological control programs. The temperature-related differences in biological traits observed in the four Trichogramma species tested hint at the importance of making careful choices regarding climatic conditions where the parasitoid is going to be used when selecting a species for biological control programs.  相似文献   

Effect of root temperature on nodule development of bean, lentil and pea     

M.de A. Lira Junior  J.R.F. Arruda 《Soil biology & biochemistry》2005,37(2):235-239

The rhizobia-legume symbiosis is the main source of fixed nitrogen for many agricultural systems. However, it is inhibited by low soil temperature. To date, research on nodulation has involved either qualitative or destructive analyses. The use of computer-based image analysis potentially allows nodules to be followed during the course of development. Seedlings of bean (Phaseolus vulgaris L.), lentil (Lens culinaris Medik.) and pea (Pisum sativum L.) were transplanted into plastic growth pouches suspended in water baths maintained at 10, 15, 20 or 25 °C. Two days after transplanting, all plants were inoculated with appropriate rhizobial strains. Seven days after inoculation, plant roots were scanned; this was repeated weekly for 7 weeks. Data on nodule length were collected through image analysis. Nodule length was correlated with nodule size and development. There were increases in the precision of estimates of environmental effects through observation of individual nodule development, as opposed to averages for populations of nodules. The effects of root temperature on nodulation and nodule development were observed both in the delayed onset of nodulation and in reduced subsequent nodule growth rate, resulting in effects on final nodule size.  相似文献   

Iron Sources Effects on Growth,Physiological Parameters and Nutrition of Cacao     

V. C. Baligar  R. C. Sicher  M. K. Elson  Z. He    J. O. De Souza Junior 《Journal of plant nutrition》2015,38(11):1787-1802

Productivity and sustainability of cacao (Theobroma cacao L.) in tropical soils are affected by levels of iron. Information is lacking on the cacao response to various sources of iron (Fe). A greenhouse experiment was conducted to evaluate the effects of five iron sources iron sulfate heptahydrate, ferric ethylenediamine-N,N’-bis(2-hydroxyphenylacetic acid), ferric diethylenetriaminepentaacetic acid, ferric ethylenediaminetetraacetic acid, fiesta herbicide (FeSO₄ · 7H₂O, FeEDDHA, FeDTPA, FeEDTA,) at 10 mg Fe kg^?1 soil on growth, photosynthesis, content of photosynthetic pigments and starch and macro- and micronutrient nutrition of cacao. The various iron sources had significant effects on shoot and root dry biomass accumulation, leaf chlorophyll a and b content, carotenoid levels, SPAD index and P_N. These parameters were significantly correlated with concentration, uptake, influx, and transport and use efficiency of Fe. In cacao net photosynthesis, stomatal conductance, internal carbon dioxide (CO₂)_, and transpiration in leaf level responded differently to the sources of Fe. Invariably, macro and micronutrient uptake, influx, transport, and use efficiency showed differential responses to sources of iron but significant effects were only observed for copper (Cu), Fe, manganese (Mn), and zinc (Zn). Overall, FeDTPA, FeEDTA and FeHEDTA could be the best sources of Fe in improving, growth, photosynthesis and macro and micro nutrition of cacao.  相似文献