Effects of lime application and test branch position on 14C-photosynthate partitioning in 3-year-old Sitka spruce (Picea sitchensis plants growing in pots containing peat soils     

Kakei  M; Clifford  PE 《Forestry》1999,72(3):223-235

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Rice phenolics efflux transporter 2 (PEZ2) plays an important role in solubilizing apoplasmic iron     

Khurram Bashir  Yasuhiro Ishimaru  Hugo Shimo  Yusuke Kakei  Takeshi Senoura  Ryuichi Takahashi 《Soil Science and Plant Nutrition》2013,59(6):803-812

Iron (Fe) is an essential micronutrient for plants; however, despite its abundance in soils it is not readily available in neutral to alkaline soils. Plants secrete phenolics, such as protocatechuic acid (PCA) and caffeic acid (CA), to absorb and utilize precipitated apoplasmic Fe from root surfaces. However, the synthesis and secretion of phenolics have not been well characterized in plants. We have identified and characterized a rice (Oryza sativa L.) mutant with reduced amounts of PCA and CA in xylem sap and root exudates; hence we named it phenolics efflux zero 2 (pez2). PEZ2 localized to the plasma membrane in onion (Allium cepa L.) epidermal cells and transported PCA when expressed in Xenopus laevis oocytes. PEZ2 expression was observed in whole root near root tips. Similarly, strong expression was observed in leaves. In line with reduced amounts of PCA and CA in xylem sap, the xylem Fe concentration was also low in pez2 plants. These results suggest that PEZ2 is involved in solubilization of apoplasmic Fe in rice.  相似文献   

Long-term effects of liming on needles, soil properties, and soil water in a Sitka spruce stand on deep peat   总被引：1，自引：0，他引：1  

Kakei  M.; Clifford  P.E. 《Forestry》2002,75(5):553-567

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Short-term effects of lime application on soil properties and fine-root characteristics for a 9-year-old Sitka spruce plantation growing on a deep peat soil     

Kakei  M.; Clifford  P.E. 《Forestry》2002,75(1):37-50

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Overexpression of the Barley Nicotianamine Synthase Gene HvNAS1 Increases Iron and Zinc Concentrations in Rice Grains   总被引：1，自引：0，他引：1  

Hiroshi Masuda  Kanako Usuda  Takanori Kobayashi  Yasuhiro Ishimaru  Yusuke Kakei  Michiko Takahashi  Kyoko Higuchi  Hiromi Nakanishi  Satoshi Mori  Naoko K. Nishizawa 《Rice》2009,2(4):155-166

In humans, iron (Fe) and zinc (Zn) deficiencies result in major worldwide health problems. Transgenic technologies to produce Fe- and Zn-biofortified rice varieties offer a promising potential solution. Nicotianamine, the precursor of phytosiderophores, chelates Fe²⁺ and Zn²⁺ and plays an important role in transporting these metals to both vegetative and reproductive organs within the plant. Our objective was to increase Fe and Zn contents in rice grains by overexpressing the barley nicotianamine synthase gene HvNAS1. HvNAS1-overexpressing transgenic rice showed increased HvNAS1 expression and subsequent increases in endogenous nicotianamine and phytosiderophore content in shoots, roots, and seeds. Fe and Zn concentrations in polished T₁ seeds from transgenic plants increased more than three and twofold, respectively; Fe and Zn concentrations also increased in both polished and brown T₂ seeds. These results suggest that the overproduction of nicotianamine enhances the translocation of Fe and Zn into rice grains.  相似文献   

Long-term effects of lime application on 15N availability to Sitka spruce seedlings growing in pots containing peat soils     

Kakei  M.; Clifford  P.E. 《Forestry》2000,73(4):393-401

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