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WANG Chun-yan Isoda Akihiro LI Mao-song WANG Dao-long 《中国农业科学(英文版)》2007,6(8):949-955
A cotton cultivar Xinluzao 8 was grown under four levels of water stress treatments （normal irrigation, slight, mild and severe water stress） from the initial reproductive growth stage in Shihezi, Xinjiang, China, in 2002, to evaluate the growth and eco-physiological performances. Under water stress conditions, the transpiration ability decreased while the leaf temperature increased. Although the relative leaf water content decreased as water stress increased, the differences among the treatments were small, indicating that cotton has high ability in maintaining water in leaf. The stomatal density increased as water stress increased, while the maximum stomatal aperture reduced only in the severest stressed plants. The time of the maximum stomatal aperture was delayed in the mild and severe stressed plants. When severe stress occurred, the stomata were kept open until the transpiration decreased to nearly zero, suggesting that the stomata might not be the main factor in adjusting transpiration in cotton. Cotton plant has high adaptation ability to water stress conditions because of decrease in both stomatal conductance and hydraulic conductance from soil-to-leaf pathway. The actual quantum yield of photosystem Ⅱ （PS Ⅱ） decreased under water stress conditions, while the maximum quantum yield of PS Ⅱ did not vary among treatments, suggesting that PS II would not be damaged by water stress. The total dry weight reduced as water stress increased. 相似文献
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Atsushi Kodama Hiroki Sakai Keiya Kobayashi Takashi Mori Kohji Maruo Tadaaki Kudo Tokuma Yanai Toshiaki Masegi 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2008,37(4):409-415
Abstract: A 1‐year‐old intact female miniature Dachshund was presented with hematochezia, vomiting, and diarrhea of more than 1‐week duration. An abdominal mass was palpated, which at exploratory surgery was found to be a 7‐cm‐long thickened section of ileum. The thickened ileum was resected. Impression smears revealed numerous small‐ to medium‐sized lymphocytes, with a smaller number of cells resembling Mott cells. The Mott‐like cells contained multiple pale vacuoles that were positive for periodic acid‐Schiff (PAS) in wet‐fixed smears, consistent with Russell bodies. Histologic evaluation of the surgically excised ileum revealed 2 populations of neoplastic lymphoid cells. The majority were uniform medium‐sized lymphocytes with hyperchromatic oval or round nuclei and inconspicuous nucleoli. The remaining cells resembled Mott cells, which contained several PAS‐positive eosinophilic globules in the cytoplasm, occasionally compressing the nucleus. The majority of neoplastic cells stained positively for vimentin, CD20, CD79a, and Pax‐5, but were negative for CD3 and lysozyme; 43.5% of cells stained positively for Ki‐67. The Mott cells were strongly positive for immunoglobulin but were negative for Pax‐5. Using electron microscopy, a homogenous substance of intermediate electron density was observed frequently in the cisternae of rough endoplasmic reticulum in the cytoplasm of the Mott cells, and rarely in the perinuclear cisternae of the lymphoid cells, corresponding to the site of immunoglobulin staining. Monoclonal rearrangement of immunoglobulin heavy‐chain (IgH) gene was observed by PCR testing for lymphocyte–antigen receptor rearrangement. The morphologic features, immunophenotype, and IgH gene rearrangement verified the lymphoid cells were neoplastic (mature cell type) and had a B‐cell phenotype, with evidence of immunoglobulin production and differentiation into Mott cells. This case was unusual because of the age of the dog and because most intestinal lymphomas are T‐cell phenotype. The Mott cell morphology also differed from typical mature B‐cell lymphoma types and may be a unique B‐cell lymphoma variant. 相似文献
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Kenichi Kashiwagi Mohamed Kefi Mohamed Ksibi Monem Kallel Atsushi Kawachi Hiroko Isoda 《农业科学与技术》2013,(9):667-676
This paper investigates the effect of the introduction of an irrigation technique on the technical efficiency of olive production in a sample of olive-growing farms in Tunisia, estimating the Cobb-Douglas form of stochastic frontier production function. Results indicate that estimated technical efficiency scores vary, ranging from a minimum of 3.0% to a maximum of 91.2% with a mean value of 61.2%. This suggests that olive-growing farms in Tunisia can increase their production on average by 38.8% through more efficient use of technology and inputs. The introduction of irrigation increased productivity, however, the estimated technical efficiency of irrigated olive farms varies across farms and they are less efficient than non-irrigated farms. This finding suggests that the introduction of irrigation to non-irrigated farms has the potential to increase production levels, however, the current production of the irrigated farms is far from the ＂best practice frontier＂ that realizes maximum possible output. Accumulation of experience and knowledge by farm owners and selection of olive cultivar significantly contribute to improving technical efficiency. 相似文献
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H Isoda H Motojima D Margout M Neves J Han M Nakajima M Larroque 《Journal of agricultural and food chemistry》2012,60(32):7851-7858
The inhibitory effect of Picholine olive oil from Montpellier in Southern France on the chemical mediator release in type I allergy, using rat basophilic leukemia (RBL-2H3) cells, was investigated. Oil-in-water (O/W) emulsions prepared using Picholine olive oil showed an inhibitory effect on the chemical mediator release and decreased expressions of genes related to type I allergy in RBL-2H3 cells. We then measured the phenolic compounds present in Picholine olive oil using high-performance liquid chromatography and investigated some physical properties, such as droplet size, size distribution, viscosity, and surface tension of the resulting olive O/W emulsions. Our findings indicate that Picholine olive oil has high flavonoids content, especially apigenin, and the prepared emulsion of Picholine olive oil resulted in a considerable small size distribution, with an average droplet size of 170 nm. 相似文献
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Ageyama N Hanazono Y Shibata H Ono F Nagashima T Ueda Y Yoshikawa Y Hasegawa M Ozawa K Terao K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(5):507-510
Genes and proteins of human origin are often administered to monkeys for research purposes, however, it can be difficult to obtain sufficient levels of the products in vivo due to immunological clearance. In this study, we showed that human erythropoietin (hEPO) induces generation of anti-hEPO antibody in cynomolgus macaques (n=2), although 92% of amino acid residues are common between the human and macaque EPO. The administered hEPO was thus eliminated from the animals. On the other hand, when an immunosuppressant, cyclosporin A (CyA), was administered (6 mg/kg) intramuscularly every other day in combination with hEPO (n=2), no anti-hEPO antibody was generated and high serum levels of hEPO were obtained during administration of hEPO, resulting in an increase in serum hemoglobin levels. No adverse effects associated with CyA were observed. Thus, CyA treatment is useful for prevention of immune responses associated with the administration of human proteins in monkeys. 相似文献
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Agaricus blazei (class Basidiomycotina) aqueous extract enhances the expression of c-Jun protein in MCF7 cells 总被引：3，自引：0，他引：3
The edible mushroom Agaricus blazei Murill is considered a health food in many countries after it was reported to be a source of antitumor and immunoactive compounds. An aqueous extract (AE) from this basidiomycete significantly enhanced the expression of the c-Jun/activator protein-1 (AP1) in the human breast cancer cell line MCF7. Incubating the cells with 17-beta-estradiol (E2), p-nonylphenol (NP), and the AE combined, or NP plus the AE, resulted in increased cell proliferation compared to the untreated control by 93 and 67%, respectively. However, incubating the cells with the extract alone did not enhance cell division. It is suggested that the enhanced proliferation of MCF7 cells in the presence of NP and the AE may be due to the involvement of an AP1 gene regulatory complex. This is the first report showing enhanced c-Jun/AP1 expression in MCF7 cells incubated with an aqueous fungal extract. 相似文献