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Kidney BA Haines DM Ellis JA Burnham ML Jackson ML 《American journal of veterinary research》2002,63(1):60-63
OBJECTIVE: To evaluate a group of vaccine site-associated sarcomas (VSS) for the presence of feline foamy virus (FeFV) DNA, using polymerase chain reaction (PCR) methods. SAMPLE POPULATION: 50 formalin-fixed paraffin-embedded (FFPE) tissue blocks from VSS of cats. PROCEDURE: DNA was extracted from FFPE sections of each tumor, and regions of the gag and pol genes of FeFV were amplified by use of PCR methods, using 1 primer set for each region. Sensitivity of the method was compared between fresh and FFPE cells, using mouse kidney tissue that was injected with FeFV-infected cultured cells and using agarose-cell pellets. Results-Feline foamy virus DNA was not detected in VSS tissues. Sensitivity of the method was 10 times greater in fresh versus FFPE mouse tissues. Sensitivity of the method in fresh FeFV-infected cultured cells versus FFPE agarose-cell pellets was equal when fixation was 24 or 48 hours and 10 times greater when fixation was 72 hours or 1 week. CONCLUSIONS AND CLINICAL RELEVANCE: A PCR-based method can be successfully applied to FFPE tissues for FeFV DNA detection. Results suggest there is no direct FeFV involvement in the pathogenesis of VSS in cats. 相似文献
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Andrea Sáenz–Arroyo Callum M. Roberts Jorge Torre & Micheline Cariño-Olvera 《Fish and Fisheries》2005,6(2):121-133
Designing fishing policies without knowledge of past levels of target species abundance is a dangerous omission for fisheries management. However, as fisheries monitoring started long after exploitation of many species began, this is a difficult issue to address. Here we show how the ‘shifting baseline’ syndrome can affect the stock assessment of a vulnerable species by masking real population trends and thereby put marine animals at serious risk. Current fishery data suggest that landings of the large Gulf grouper (Mycteroperca jordani, Serranidae) are increasing in the Gulf of California. However, reviews of historical evidence, naturalists’ observations and a systematic documentation of fishers’ perceptions of trends in the abundance of this species indicate that it has dramatically declined. The heyday for the Gulf grouper fishery occurred prior to the 1970s, after which abundance dropped rapidly, probably falling to a few percent of former numbers. This decline happened long before fishery statistics were formally developed. We use the case of the Gulf grouper to illustrate how other vulnerable tropical and semi‐tropical fish and shellfish species around the world may be facing the same fate as the Gulf grouper. In accordance with other recent studies, we recommend using historical tools as part of a broad data‐gathering approach to assess the conservation status of marine species that are vulnerable to over‐exploitation. 相似文献
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Michael Simone-Finstrom Micheline K Strand David R Tarpy Olav Rueppell 《Journal of insect science (Online)》2022,22(1)
The effects of honey bee management, such as intensive migratory beekeeping, are part of the ongoing debate concerning causes of colony health problems. Even though comparisons of disease and pathogen loads among differently managed colonies indicate some effects, the direct impact of migratory practices on honey bee pathogens is poorly understood. To test long- and short-term impacts of managed migration on pathogen loads and immunity, experimental honey bee colonies were maintained with or without migratory movement. Individuals that experienced migration as juveniles (e.g., larval and pupal development), as adults, or both were compared to control colonies that remained stationary and therefore did not experience migratory relocation. Samples at different ages and life-history stages (hive bees or foragers), taken at the beginning and end of the active season, were analyzed for pathogen loads and physiological markers of health. Bees exposed to migratory management during adulthood had increased levels of the AKI virus complex (Acute bee paralysis, Kashmir bee, and Israeli acute bee paralysis viruses) and decreased levels of antiviral gene expression (dicer-like). However, those in stationary management as adults had elevated gut parasites (i.e. trypanosomes). Effects of environment during juvenile development were more complex and interacted with life-history stage and season. Age at collection, life-history stage, and season all influenced numerous factors from viral load to immune gene expression. Although the factors that we examined are not independent, the results illuminate potential factors in both migratory and nonmigratory beekeeping that are likely to contribute to colony stress, and also indicate potential mitigation measures. 相似文献
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Nitrogen management for production of bread quality wheat ( Triticum aestivum L.) in eastern Canada has received little research attention. An experiment was conducted for 2 years at each of two sites in Québec to study the effect of level and timing of nitrogen (N) fertilizer application on grain protein concentration, protein content per seed, non-protein seed dry matter, grain protein yield and nitrogen harvest index (portion of plant N in the grain) of four hard red spring wheat cultivars known to have potential as bread wheats in eastern Canada. The soil types were Bearbroock clay (fine, mixed, non-acid, frigid, Humaquept) and Ste-Rosalie clay (typic, non-acid, frigid, Humaquept). The experiment was a 4 × 4 × 2 factorial. Four cultivars were used: Columbus, Katepwa, Max and Hege 155–85. In both years 0, 60, 120 and 180 kg Nha−1 were applied either all at seeding or 60 % at seeding and 40 % at heading. Grain protein concentration and grain protein yield increased consistently with increasing N fertilizer and with split N application. Nitrogen harvest index was not increased by increasing applications of N fertilizer. Protein content per seed was more critical in determining grain protein concentration than non-protein seed dry matter content. The western Canadian cultivars Columbus and Katepwa generally had greater grain protein concentration than the European cultivars Max and Hege 155–85, With reasonable N fertility the grain protein concentration of spring wheats grown in eastern Canada are sufficient for bread production. 相似文献
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The influence of landscape features on the movement of an organism between two point locations is often measured as an effective distance. Typically, raster models of landscape resistance are used to calculate effective distance. Because organisms may experience landscape heterogeneity at different scales (i.e. functional grains), using a raster with too fine or too coarse a spatial grain (i.e. analysis grain) may lead to inaccurate estimates of effective distance. We adopted a simulation approach where the true functional grain and effective distance for a theoretical organism were defined and the analysis grains of landscape connectivity models were systematically changed. We used moving windows and grains of connectivity, a recently introduced landscape graph method that uses an irregular tessellation of the resistance surface to coarsen the landscape data. We then used least-cost path metrics to measure effective distance and found that matching the functional and analysis grain sizes was most accurate at recovering the expected effective distance, affirming the importance of multi-scale analysis. Moving window scaling with a maximum function (win.max) performed well when the majority of landscape structure influencing connectivity consisted of high resistance features. Moving window scaling with a minimum function (win.min) performed well when the relevant landscape structure consisted of low resistance regions. The grains of connectivity method performed well under all scenarios, avoiding an a priori choice of window function, which may be challenging in complex landscapes. Appendices are provided that demonstrate the use of grains of connectivity models. 相似文献
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Enrichment of anhydrous milk fat in polyunsaturated fatty acid residues from linseed and rapeseed oils through enzymatic interesterification 总被引：1，自引：0，他引：1
Aguedo M Hanon E Danthine S Paquot M Lognay G Thomas A Vandenbol M Thonart P Wathelet JP Blecker C 《Journal of agricultural and food chemistry》2008,56(5):1757-1765
Lipozyme TL IM was used in a solvent-free batch and microaqueous system for enzymatic interesterification of anhydrous milkfat (AMF) with linseed oil (LO) in binary blends and with rapeseed oil (RO) in one ternary blend. The aim was to obtain and characterize physicochemically fats enriched with unsaturated C 18 fatty acids (oleic, linoleic, and, especially, linolenic acids) from natural vegetable oils. Binary blends of AMF/LO 100/0, 90/10, 80/20, 70/30, and 60/40 (w/w) were interesterified. The change in triacylglycerol (TAG) profiles showed that quasi-equilibrium conditions were reached after 4-6 h of reaction. Free fatty acid contents <1%. The decrease in solid fat content and in dropping point temperature obtained with increasing content of LO and interesterification resulted in good plastic properties for the products originating from the blends 70/30 and 60/40. This was confirmed by textural measurements. Melting profiles determined by differential scanning calorimetry showed complete disappearance of low-melting TAGs from LO and the formation of intermediary species with a lower melting temperature. Oxidative stability of the interesterified products was diminished with increasing LO content, resulting in low oxidation induction times. A ternary blend composed of AMF/RO/LO 70/20/10 gave satisfactory rheological and oxidative properties, fulfilling the requirements for a marketable spread and, moreover, offering increased potential health benefits due to the enriched content in polyunsaturated fatty acid residues. 相似文献
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Evaluation of N Fertilizer Rate and Timing and Wheat Cultivars on Soil Residual Nitrates 总被引：3，自引：0，他引：3
Nitrogen (N) fertilizer management for production of bread quality wheat may increase nitrate residues in the soil. To assess soil nitrate levels associated with bread quality wheat ( Triticum aestivum L.) production in Eastern Canada, an experiment was conducted for 2 years at each of two sites in Québec. Four cultivars (Columbus, Katepwa, Max and Hege 155-85), four N levels (0, 60, 120 and 180 kg N ha−1 ) and two N timings (all at seeding time or 60 % at seeding and 40 % at anthesis) were combined in a factorial arrangement on a Bearbrook clay and a Ste-Rosalie clay. Residual soil NO3 -N levels were measured in the 0–20 and 20– 60 cm soil layers. The cultivars used have potential as bread wheats. Cultivar effects on soil nitrate levels existed only in the Ste-Rosalie soil, suggesting that the cultivars used were better adapted to the conditions on the Bearbrook soil. Changes in soil NC3 N levels over winter indicated that mineralization had occurred. Calculated balance-sheet values were larger than measured residual NO3 -N in the autumn of each year, indicating that NO3 -N was lost from the systems, possibly due to denitrification. Potential increases in, and thus potential pollution from, residual soil NO-N existed only at the 180 kg N ha−1 level. Overwinter changes in soil NO3 -N levels were proportional to the inverse of the fall NC3 N levels. Differences between sites were large for many of the variables measured. 相似文献
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Tour guides as tourist products in Dalat,Vietnam: Exploring market freedoms in a communist state 
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下载免费PDF全文 This paper investigates how tour guides position themselves in Vietnam's transition from a command economy to market socialism. The case study of the Dalat Easy Riders motorcycle guides demonstrates that tour guides take advantage of, personify and contribute to the economic, political and social transitions in the country. Relatedly, we argue that Vietnam's transition (known as ??i m?i) is a key component of the Easy Riders' promotional narratives. In sum, we analyse how tour guides become tourist products in a transitional society. 相似文献
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