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B. Shiran N. Amirbakhtiar S. Kiani Sh. Mohammadi B.E. Sayed-Tabatabaei H. Moradi 《Scientia Horticulturae》2007
RAPDs and SSRs were used to study the genetic diversity of Iranian almond cultivars and their relationship to important foreign cultivars and three related species. Eight unidentified almond Shahrodi cultivars and three wild almonds (Prunus communis, Prunus orientalis and Prunus scoparia) were also included. Of the primers tested, 42 (out of 80) RAPD and 18 (out of 26) SSR primers were selected for their reproducibility and high polymorphism. A total of 664 polymorphic RAPD bands were detected out of 729 bands. The number of presumed alleles revealed by the SSR analysis ranged from 3 to 10 alleles per locus with a mean value of 6.64 alleles per locus. Both techniques discriminated the genotypes very effectively, but only RAPDs were able to discriminate the cultivars Monagha and Sefied. Results demonstrated an extensive genetic variability within the tested cultivars as well as the value of SSR markers developed in peach for characterization of almond and related species of Prunus. Dice similarity coefficient was calculated for all pair wise comparisons and was used to construct a UPGMA dendrogram. For both markers a high similarity in dendrogram topologies was obtained although some differences were observed. All dendrograms, including that obtained by the combined use of both the marker data, depicted the phenetic relationships among the cultivars and species, depending upon their geographic region and/or pedigree information. Almond cultivars clustered with accession of P. communis showing their close relationship. P. orientalis and P. scoparia were clustered out of the rest of P. dulcis. 相似文献
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Nazanin Ghavami Hossein Ali Alikhani Ahmad Ali Pourbabaee Hossein Besharati 《Communications in Soil Science and Plant Analysis》2016,47(12):1517-1527
Plant growth-promoting rhizobacteria (PGPR) are soil bacteria that are able to colonize rhizosphere and to enhance plant growth by means of a wide variety of mechanisms. In the present study, Myristica yunnanensis and Stenotrophomonas chelatiphaga strains were recognized as new records in Iran flora. According to the results, these strains significantly affected plants’ zinc and phosphorous contents which could be due to the production of phytosiderophore. Siderophore-producing bacteria increased canola zinc (Zn) content as strategy-I plant, while in maize, it can be said that probably the effect of phytosiderophore produced by plant on increasing root and shoot Zn content was more than siderophore produced by bacteria. These isolates could be used as bio-input for improving the plant productivity as a substitute to chemical fertilizers and also to correct the nutrient deficiencies in canola and maize for sustainable agriculture. 相似文献
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Obesity subjects individuals into metabolic and endocrine disorders. Thus obesity may increase the risk of vitamin D deficiency. This text aims at studying the prevalence of vitamin D deficiency and secondary hyperparathyroidism in obese children. In a non-randomized case control study on 52 obese children (body mass index (BMI) >95th percentile) aged 4 to 16 years undertaken at the outpatient endocrine clinic of the Children Hospital at Tabriz University between 2009-2011. This study was conducted to compare the prevalence of vitamin D deficiency and secondary hyperparathyroidism in obese children compared with 57 non obese (BMI < 85th percentile). 109 children including 52 (50.5%) boys and 57 (49.5%) girls were studied. Most of case (76.9%) and control (42.1%) groups suffered from degrees of vitamin D deficiency. There was meaningful statistical difference between two groups considering to vitamin D deficiency and parathyroid hormone (p = 0.001). A negative relations was found between iPTH and vit D level (p < 0.001, r = -0.2), BMI and 25-OH vit D (p < 0.001, r = -0.2). A positive relation was observed between parathyroid hormone and BMI (p = 0.009, r = 0.1). Obese children are at high risk at vitamin D deficiency and secondary hyperparathyroidism. BMI appears to be an important risk factor for vitamin D deficiency. 相似文献
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Samaneh Saberi Kazem Zendehdel Sahar Jahangiri Yeganeh Talebkhan Afshin Abdirad Nazanin Mohajerani Maryam Bababeik Najmeh Karami Maryam Esmaili Akbar Oghalaie Parisa Hassanpour Neda Amini Mohammad Ali Mohagheghi Mahmoud Eshagh Hossieni Marjan Mohammadi 《Iranian Biomedical Journal》2012,16(4):179-184
Background
Attempts for early detection of gastric cancer have recently focused on host''s genetic susceptibility factors and gene-environment interactions. We have, herein, studied the association of MTHFR C677T single nucleotide polymorphism (SNP) and its interaction with Helicobacter pylori infection, smoking, age and gender on the risk of gastric cancer among an Iranian population.Methods
Gastric cancer patients (n = 450) and cancer-free controls (n = 780) were studied for serum H. pylori-specific IgG antibodies by ELISA and MTHFR C677T polymorphism (SNP) by PCR-RFLP. Demographic and life style data were collected through patient interviews. Unconditional logistic regression model estimated odds ratio (OR) and the corresponding 95% confidence intervals (CI).Results
The interactions of MTHFR genotype with H. pylori infection (P = 0.03), age (P = 0.049) and gender (P = 0.007) were statistically significant. Accordingly, MTHFR C677T carriers who were also positive for H. pylori infection exhibited 80% (OR = 1.8, 95% CI = 1.0-2.9) significant excess risk of non-cardia gastric cancer. Furthermore, subjects over the age of 50 or female subjects carrying MTHFR C677T SNP showed 40 (OR = 1.4, 95% CI = 1.0-2.0) and 100 (OR = 2.0, 95% CI = 1.2-3.2) percent increased risk of gastric cancer, respectively.Conclusion
MTHFR C677T SNP seems to increase the risk of gastric cancer and the effect is significantly inflated by interactions with H. pylori infection, age and gender. Key Words: Helicobacter pylori, Smoking, Gender identity, Age group, Methylenetetrahydrofolate reductase 相似文献6.
Nazanin Ghavami Hossein Ali Alikhani Ahmad Ali Pourbabaei Hossein Besharati 《Journal of plant nutrition》2017,40(5):736-746
Siderophore-producing rhizobacteria beneficially affect plant growth by providing available iron to plants. In this study, bacteria were isolated from the rhizosphere of canola (Brassica napus L.) plants grown in the central fields in Iran, for the presence of siderophore-producing bacteria. A total of 45 distinct isolates were found to produce siderophore using qualitative chrome azurol sulfonate (CAS)-agar assay. Of them, ten isolates, based on the highest halo diameter/colony diameter ratios, were selected to quantify the rate of siderophore production using CAS-liquid assay. A variety of biochemical assays was used to determine the type(s) of siderophores produced by each of the ten isolates. The best isolates, based on production of the highest rates of either hydroxamates or carboxylates, were identified and used in further studies. Based on 16S ribosomal ribonucleic acid (rRNA) sequence analysis and a variety of phenotypic properties, the isolates were identified as Micrococcus yunnanensis YIM 65004 (T) and Stenotrophomonas chelatiphaga LPM-5 (T). We also studied the plant growth-promoting effect of the most promising isolates (YIM 65004 and LPM-5) on canola and maize plants under greenhouse conditions. The results of this study showed that M. yunnanensis and S. chelatiphaga increased gain weight and iron (Fe) content of roots and shoots significantly, in comparison with control, indicating beneficial effects of these rhizobacteria on plant growth and development. This study reports M. yunnanensis and S. chelatiphaga, as new records for Iran. The latter is reported for the first time from plant (canola) rhizosphere. Besides, the ability of both M. yunnanensis and S. chelatiphaga to produce siderophores is documented for the first time. 相似文献
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分子标记法和形态学特征对杏仁栽培种及野生种基因型的遗传关系构建的辨别 总被引：1，自引：0，他引：1
Karim Sorkheh Behrouz Shiran Soghra Kiani Nazanin Amirbakhtiar Sadegh Mousavi Vahid Rouhi Shahram Mohammady-D Thomas M.Gradziel Lyudmyla V.Malysheva-Otto Pedro Martínez-Gómez 《林业研究》2009,20(3):183-194
应用23个形态学特征,19个扩增片段长度多态性(AFLP)引物组合,80个随机扩增多态DNA(RAPD)引物和32个简单序列重复(SSR)引物对,比较三种分子标记法在29个杏仁栽培种和3个野生种遗传关系构建中的信息景和效率.根据预期杂合度的评价,与AFLPs和RAPDs相比,SSRs具有较高水平的多态性和较大的信息量.AFLPs预期朵合度值最低,但其辨别效率值最高,因为AFLPs能揭示每个反应中的大量条带,导致各种类型的多样性指数均较高.三种分子标记法对杏仁基因型的辨别效率均较高,只是SSRs无法辨别‘Monagha'和‘Sefied'杏仁基因型.三种分子标记法基因型相似性相关系数统计上显著,但SSR数据要低于RAPDs和AFLPs的值.尽管三种分子标记法树形图拓扑结构存在一些差异,但相似性水平均较高.SSRs、RAPDs和AFLPs的系统树图及其综合数据都能依据地理散布反映大多数栽培种的关系.AMOVA检测到每个地理组中栽培种和野生种的变异.辅助程序分析表明,实验所应用的标记物数量足以保证基因相似性估计的可靠性和标记法间的比较是有意义的. 相似文献
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