A coat protein transgene from a Scottish isolate of potato mop-top virus mediates strong resistance against Scandinavian isolates which have similar coat protein genes     

Brian Reavy  Maria Sandgren  Hugh Barker  Pekka Heino  Per Oxelfelt 《European journal of plant pathology / European Foundation for Plant Pathology》1997,103(9):829-834

Resistance tests were made on seedlings of transformed lines of Nicotiana benthamiana which contain a transgene encoding the coat protein (CP) gene of a Scottish isolate of potato mop-top virus (PMTV). This transgene has been reported to confer strong resistance to the PMTV isolate from which the transgene sequence was derived and also to a second Scottish isolate. Plants of lines of the transgenic N. benthamiana were as resistant to two Swedish and two Danish PMTV isolates as to a Scottish isolate, and of five lines tested, greater than 93.5% of transgenic plants were immune. The coat protein gene sequences of these four Scandinavian isolates were very similar to those of the two Scottish isolates. The greatest divergence between the isolates was three amino acid changes and there was less than 2% change in CP gene nucleotide sequence. It is concluded that the PMTV CP transgene used in these experiments could confer resistance against isolates from different geographical areas because it is becoming apparent that the CP genes of PMTV isolates are highly conserved.  相似文献   

玉米青贮与花生秧配比对奶牛瘤胃中花生秧降解特性的影响     

秦雯霄  廉红霞  付彤  高腾云  孙宇  李改英 《动物营养学报》2015,(6)

本试验旨在研究玉米青贮与花生秧配比对奶牛瘤胃中花生秧降解特性的影响。选择4头体重、生理状态、生产性能相近,装有永久瘤胃瘘管的中国荷斯坦奶牛,分别饲喂含有玉米青贮与花生秧不同配比的全混合日粮(TMR),3种TMR中玉米青贮与花生秧的干物质(DM)配比分别为3.9∶1.0(A组)、1.2∶1.0(B组)、0.4∶1.0(C组)。试验分3期进行,依次进行A、B、C组试验。每期预试15 d,采样期4 d;共57 d。采用尼龙袋瘤胃降解技术测定花生秧在奶牛瘤胃中DM、粗蛋白质(CP)、中性洗涤纤维(NDF)和酸性洗涤纤维(ADF)的72 h瘤胃降解率,并求得目标养分的动态降解参数及有效降解率。结果表明:1)花生秧的DM在瘤胃中有效降解率为56.49%~59.62%,CP为40.45%~47.36%,NDF为33.26%~35.20%,ADF为36.31%~37.45%。2)B组DM的有效降解率显著高于C组(P0.05),极显著高于A组(P0.01);B、C组的CP有效降解率显著高于A组(P0.05)。3)B、C组的NDF快速降解部分含量显著高于A组(P0.05);3种TMR对花生秧粗NDF和ADF有效降解率无显著影响(P0.05)。由此可见,花生秧具有较高的饲用价值。本试验条件下,玉米青贮与花生秧DM配比为1.2∶1.0时,可有效提高花生秧DM和CP的瘤胃降解率。  相似文献   

Changing stock of biomass carbon in a boreal forest over 93 years     

Pekka E. Kauppi  Aapo Rautiainen  Kari T. Korhonen  Aleksi Lehtonen  Jari Liski  Pekka Nöjd  Sakari Tuominen  Markus Haakana  Tarmo Virtanen 《Forest Ecology and Management》2010,259(7):1239-1244

The growing stock more than doubled from 1.6 to 3.4 million m³ between 1912 and 2005 in forests on an area of 387 km² in southern Finland. The stock expansion continued for 93 years noting interim results, which were available for 1959, 1982, 1994 and 1999. Forested area in the region hardly changed. Carbon sequestration was mainly a result of a long-term recovery from forest degradation, a legacy of land use in the 18th and 19th centuries. Tree demography responded to management change especially of mature stands: Average tree size and stocking density of stands increased. On average the expanding biomass stock sequestered 18 tons C annually per km² (18 g C per m²). In comparison, the emissions of fossil carbon in the region were estimated at 12 tons C per km² (12 g C per m²) on average. However, fossil CO₂ emissions exceeded biomass sequestration in recent decades. The powerful and persistent expansion of the carbon stock was an unintended co-benefit of forestry, which was motivated by the intention to improve timber yield. On the more negative side the change in management introduced clear-cuts, and a loss of diverse elements of the pre-industrial biota.  相似文献   

当前广西柑橘无病苗木生产和推广工作中应当重视的几个问题     

莫健生  刘升球  邓光宙  陈传武  付慧敏 《广西园艺》2007,18(5):11-14

在2005年12月由广西区农业厅和桂林市人民政府联合举办的“全区成功防治柑桔黄龙病交流培训会”的推动下，全区各地都将柑桔无病苗木生产提到了政府议事日程上，一些主产区已建起了自己的无病苗圃，开始柑桔无病苗木的生产。2006年国家农业部下达“广西桂林市柑桔无病良种繁育基地”项目由广西柑桔研究所承担。  相似文献   

三个以色列水果黄瓜品比试验初报     

张兴无  李向林  高安辉  黄静 《广西园艺》2007,18(5):26-27

引进以色列黄瓜新星(NOVA)、伯金(BERGKAMP)和萨娜(SANA)三个水果黄瓜品种在罗甸进行栽培比较试验,结果表明新星(NOVA)单瓜最重,产量最高,抗性最强,适合在贵州南亚热带栽培.  相似文献   

蔬菜生产在土壤重金属污染治理中的作用   总被引：3，自引：0，他引：3  

朱勇  江潇潇  许秀琴  叶宇飞 《长江蔬菜》2007,(10):31-33

由于人们对工业高度发达的负面影响预料不够,预防不利,导致污染事件时有发生.但污水、黑烟、臭气都能被人能直接的感觉到,而土壤污染是看不见的一种污染,它往往不能给人以直接的感受,而是通过其他途径来表现,如粮食减产、绝收,蔬菜产量品质下降.  相似文献   

昆虫性信息素群集诱杀技术对斜纹夜蛾的控害研究   总被引：2，自引：0，他引：2  

覃保荣  黄庆文  陈丽丽  陈斌艳  何燕  黎美坤 《广西植保》2007,20(3):4-7

试验结果表明,与常规防治相比,昆虫性信息素群集诱杀技术具有安全、环保、长效的控害作用,防治效果与成本均优于常规防治;不同放置密度、高度对斜纹夜蛾诱杀效果有差异,高密度区诱蛾量及对减少田间落卵量、压低田间虫源等作用方面表现较好,但成本相对较高,诱捕器悬挂高度以距离地面80~100cm诱蛾效果较好,挂放10d内及发蛾高峰期诱蛾效果明显。  相似文献   

6类9种杀虫剂对稻飞虱的田间效果综合评价     

彭超美  孙光忠  姜干明  董兰  许凌风 《湖北植保》2007,(5):27-29

水稻稻飞虱是我国和许多亚洲国家当前水稻生产中最主要的害虫,也是湖北省水稻生产上的主要迁飞性害虫之一。近几年来我省连续特大发生。特别是2006年。为了明确防治稻飞虱当家农药品种和不同剂型的田间实际防治效果,选择高效、环保、低毒和安全的农药品种推广应用,我们于2006年  相似文献   

籼爪交中稻新组合特优671的选育与应用   总被引：3，自引：1，他引：2  

陈若平  黄华康  杨卓飞  刘兆平  陈华  尤光熙  陈芦根 《福建稻麦科技》2007,25(3):1-3

利用籼爪杂交育种技术育成亚恢671,与龙特甫A配组育成亚种间杂交中稻新组合特优671,具有高产、稳产、适应广等特点,2004年通过福建省农作物品种审定委员会审定。  相似文献   

Polymerisation of added coniferyl alcohol by inherent xylem peroxidases and its effect on fungal decay resistance of Norway spruce     

Sanni Raiskila  Kurt Fagerstedt  Tapio Laakso  Pekka Saranpää  Mia Löija  Leena Paajanen  Riitta Mahlberg  Anne-Christine Ritschkoff 《Wood Science and Technology》2006,40(8):697-707

It is known that active peroxidase isozymes exist in mature wood of Norway spruce (Picea abies L. Karst.) and that they remain active for years and are found even in the heartwood (in Scots pine), where all cellular activity has ceased. This peroxidase activity was utilised in the impregnation of wood blocks with a natural monolignol, coniferyl alcohol and hydrogen peroxide. The hypothesis was that the internal wood peroxidases would oxidise the added monolignol and bind it stably into the cell wall matrix, which could hinder fungal decay. Since coniferyl alcohol is not very soluble in water, the impregnation was done under vacuum with an acetone–water solution containing 10% coniferyl alcohol and 0.4 mM H₂O₂ at room temperature (ca. 0.02 g of coniferyl alcohol was added to 1 g of wood). After impregnation, dimers of coniferyl alcohol and free coniferyl alcohol were found in acetone extracts with GC–MS analysis. Penetration of coniferyl alcohol and non-extractable reaction products were studied from the wood blocks with FTIR PAS technique. The wood samples treated were also subjected to a fungal decay test with Coriolus versicolor. This treatment hindered fungal decay in a 60-day experiment and led to a dry weight loss of 8.8% in comparison with 19.9% in the control. The reactions of coniferyl alcohol and H₂O₂ in the presence of peroxidases are discussed as well as the use of monolignols to increase wood decay resistance.  相似文献