Upper body movement analysis of multiple limb asymmetry in 367 clinically lame horses     

Suphannika Phutthachalee  Kathrin Mählmann  Suvaluk Seesupa  Christoph Lischer 《Equine veterinary journal》2021,53(4):701-709

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Comparative infectivity for axenic and specific-pathogen-free chickens of O2 Escherichia coli strains with or without virulence factors   总被引：1，自引：0，他引：1  

A Brée  M Dho  J P Lafont 《Avian diseases》1989,33(1):134-139

Adhesion to epithelial respiratory cells, iron acquisition, and production of K1 polysaccharide capsules have been proposed as potential virulence factors of avian Escherichia coli. These factors were studied by inoculating groups of axenic or specific-pathogen-free (SPF) chickens intratracheally with O2 E. coli strains after previous challenge with a wild strain of infectious bronchitis virus (IBV). In all experiments, the association between IBV and an E. coli strain endowed with the three virulence factors previously mentioned resulted in the most severe pathological effects, as measured by mortality, weight gains, lesions, and reisolation of E. coli from internal organs. An E. coli strain devoid of virulence factors was able only to induce mild pathological effects restricted to the respiratory tract when combined with IBV. Both E. coli strains were more invasive in axenic chickens than in SPF chickens. These results confirm the probable involvement of the three factors studied in the pathogenic properties of avian E. coli. This model can be used to assess the role of virulence factors, by comparing pairs of positive and negative isogenic strains.  相似文献   

Verbesserung der Krankheitsresistenz von Kohlgemüse: 2. Kohlhernie, Alternaria- und Phoma-Blattfleckenkrankheit     

Paul Scholze  Reiner Krämer  Frank Marthe  Ulrich Ryschka  Evelyn Klocke  Günter Schumann 《Gesunde Pflanzen》2003,55(7):199-204

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Tentative description of Hippeastrum latent virus in Hippeastrum hybridum plants and differentiation from Hippeastrum mosaic virus     

J. E. Brölman-Hupkes 《European journal of plant pathology / European Foundation for Plant Pathology》1975,81(6):226-236

In mosaic-diseased plants ofHippeastrum hybridum two viruses were found. One virus with a normal length of 706 nm caused local lesions onHyoscyamus niger test plants and mosaic symptoms in the leaves ofH. hybridum. This virus was identified with theHippeastrum mosaic virus (HMV) (*/*∶*/*∶E/E∶S/*) and had a dilution end point between 10^?3 and 10^?4, a thermal inactivation point between 55–60°C and a longevity at room temperature of 28–32 hours. The second virus had a normal length between 584 and 611 nm depending on the method used. It caused local lesions onGomphrena globosa andChenopodium quinoa leaves, and after inoculation ofH. hybridum was found to be present without showing symptoms. It was readily purified from inoculated leaf tissue ofC. quinoa andNicotiana clevelandii by differential centrifugation and ofH. hybridum by density-gradient centrifugation. Purified virus had an absorption minimum at 242 nm, a maximum at 262 nm and a 260/280 absorption ratio of 1.19. The dilution end point was between 10^?3 and 10^?4, the thermal inactivation point between 70 and 80°C and the longevity in vitro at room temperature 28–32 hours. Although no direct comparisons have been made with other members of the potexvirus group, the virus seems to be a new one now namedHippeastrum latent virus. Both viruses were not seed-borne.  相似文献   

Möglichkeiten zur Förderung und zum Einsatz von Nützlingen an Stadtbäumen     

Barbara Jäckel  Hartmut Balder  Kristina Hasselmann 《Gesunde Pflanzen》2002,54(7):218-226

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Bedeutung von Regenwurmröhren für die Verlagerung des Herbizids Diuron in Böden von Obstanlagen     

S. Pätzold  G. W. Brümmer 《Erwerbs-Obstbau》2004,46(3):74-80

Herbicide translocation was investigated in a Haplic Luvisol from loess (clayey silt; Klein-Altendorf near Bonn, Germany) and a Eutric Cambisol (sandy loam; fluvial sediments on the lower Rhine terrace north of Bonn) using the herbicide diuron as an example. diuron was applied once a year in May at the recommended dose of 2.4 kg/ha. It rapidly degraded in the topsoil: the “time for 50% disappearance” (DT₅₀) was 14–30 days. A population of adapted soil micro-organisms, which is repeatedly exposed to applications of the same compound every year, is thought to be responsible for this fast degradation. Remaining Diuron residues were largely degraded in the loess soil during the subsequent summer and became less mobile due to increasing binding strength. As a result, residue levels and leaching in the loess soil during the winter were negligible, with diuron traces of less than 10 µg/kg below the topsoil 11 months after application. Comparison of the translocation in a sandy loam soil showed—as expected—that diuron was leached to the subsoil to a higher extent than in clayey silt (loess). However, in the loess soil, diuron leached to the subsoil via macropores after 20 mm of natural precipitation within 1 week after herbicide application due to macropore flow. Colour tracer and analysis of the linings of earthworm burrows identified these macropores as preferred pathways of herbicide leaching. Macropore flow occurred in wet soil and moist soil with a sealed surface or crust but not in dry soil. Superficial mechanical soil cultivation before the herbicide application is discussed as one possibility to interrupt macropore entries and reduce herbicide leaching after its application in the spring.  相似文献   

The potential for the Rapid Screening of Potato Cultivars (Solanum tuberosum) for Resistance to Powdery Scab (Spongospora subterranea) using a Laboratory Bioassay   总被引：2，自引：0，他引：2  

U. Merz  V. Martinez  R. Schwärzel 《European journal of plant pathology / European Foundation for Plant Pathology》2004,110(1):71-77

Powdery scab of potato, once established in a field, is difficult to control because of the longevity of the resting spores (cystosori) of the causal organism, Spongospora subterranea f.sp. subterranea. Host resistance is likely to be the most efficient in a long-term control strategy for preventing build-up of field inoculum and spread of the disease. Resistance screening of potato cultivars is mostly done in laborious field trials where disease development is likely to be unpredictable. A bioassay with potato tissue cultured plantlets and cystosori as inoculum is described and was tested for its potential to screen potato cultivars at an early stage for their relative susceptibility to powdery scab by comparing the lab results with field data. With cystosori inoculum of Swiss origin, the laboratory test showed clear differences between the potato cultivars in the severity of zoosporangial root infection which correlated better with ranked tuber infection data, compared to root galling. There are apparent differences in the relative trends in susceptibility between roots and tubers of five selected cultivars when using naturally infested soil instead of prepared cystosori as inoculum in the lab bioassay. Furthermore, differences in the severity of zoosporangial root infection of two selected cultivars were found when cystosori from different countries where used as inoculum. A possible host genotype × pathogen interaction is discussed. The bioassay has the potential to screen and select for resistant material at an early breeding stage thus making field trials not unnecessary but more economical. It will allow the use of a standard set of pathogen collections and facilitate testing for inoculum virulence in infested soils.  相似文献   

Effects of dobutamine,dopamine, phenylephrine and noradrenaline on systemic haemodynamics and intestinal perfusion in isoflurane anaesthetised horses          下载免费PDF全文

C. Dancker  K. Rohn  S. B. Kästner 《Equine veterinary journal》2018,50(1):104-110

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Genetic diversity and historic introgression in German Angler and Red Dual Purpose cattle and possibilities to reverse introgression     

J. Schäler  R. Wellmann  J. Bennewitz  G. Thaller  D. Hinrichs 《Acta Agriculturae Scandinavica, Section A - Animal Sciences》2018,68(2):63-72

Recovering native uniqueness has major importance for breeds with historic introgression. The aim of the study was to estimate population genetic parameters for two local red cattle breeds from Northern Germany and to study possibilities to reverse introgression. Genealogical information consisted of 90,783 individuals for German Angler and 187,255 individuals for Red Dual-Purpose cattle breed, with additional information on sex, born, breed, status, and conventional breeding values. It is concluded that the native genetic contribution could be included as an additional trait in the total merit index in order to recover a part of the native genetic background. Native contributions should be estimated in the long term from marker data in order to account for Mendelian sampling. The maintenance of a sufficient genetic diversity of native alleles can be achieved by an advanced OCS with appropriate constraints.  相似文献   

CD117‐ and vimentin‐positive telocytes in the bovine teat sphincter          下载免费PDF全文

M. G. Wagener  S. Leonhard‐Marek  J.‐D. Häger  C. Pfarrer 《Anatomia, histologia, embryologia》2018,47(3):268-270

Mastitis is a common economically relevant problem in dairy farming. As the major entry for pathogens is the papillary duct, one of the first defence mechanisms is the teat sphincter. This sphincter shows a rhythmic contractility of yet unknown origin. Searching for possible modulatory pacemaker cells, teat sphincters of eight cows were stained immunohistochemically with antibodies against CD117 and vimentin and evaluated microscopically for the presence of telocytes. CD117‐ and vimentin‐positive telocytes with telopodes were found in close contact with smooth muscle cells. Our findings present a first evidence of telocytes in the teat of bovines.  相似文献