Expressed sequence tag of Japanese flounder Paralichthys olivaceus skin cells     

Ikuo  HIRONO  Ryosuke  YAZAWA  Takashi  AOKI 《Fisheries Science》2004,70(1):195-197

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First Identification of 12β-Deoxygonyautoxin 5 (12α-Gonyautoxinol 5) in the Cyanobacterium Dolichospermum circinale (TA04) and 12β-Deoxysaxitoxin (12α-Saxitoxinol) in D. circinale (TA04) and the Dinoflagellate Alexandrium pacificum (Group IV) (120518KureAC)     

Michiru Akamatsu  Ryosuke Hirozumi  Yuko Cho  Yuta Kudo  Keiichi Konoki  Yasukatsu Oshima  Mari Yotsu-Yamashita 《Marine drugs》2022,20(3)

Saxitoxin and its analogues, paralytic shellfish toxins (PSTs), are potent and specific voltage-gated sodium channel blockers. These toxins are produced by some species of freshwater cyanobacteria and marine dinoflagellates. We previously identified several biosynthetic intermediates of PSTs, as well as new analogues, from such organisms and proposed the biosynthetic and metabolic pathways of PSTs. In this study, 12β-deoxygonyautoxin 5 (12α-gonyautoxinol 5 = gonyautoxin 5-12(R)-ol) was identified in the freshwater cyanobacterium, Dolichospermum circinale (TA04), and 12β-deoxysaxitoxin (12α-saxitoxinol = saxitoxin-12(R)-ol) was identified in the same cyanobacterium and in the marine dinoflagellate Alexandrium pacificum (Group IV) (120518KureAC) for the first time from natural sources. The authentic standards of these compounds and 12α-deoxygonyautoxin 5 (12β-gonyautoxinol 5 = gonyautoxin 5-12(S)-ol) were prepared by chemical derivatization from the major PSTs, C1/C2, produced in D. circinale (TA04). These standards were used to identify the deoxy analogues by comparing the retention times and MS/MS spectra using high-resolution LC-MS/MS. Biosynthetic or metabolic pathways for these analogues have also been proposed based on their structures. The identification of these compounds supports the α-oriented stereoselective oxidation at C12 in the biosynthetic pathway towards PSTs.  相似文献   

Occurrence and Distribution of Perfluorooctane Sulfonate and Perfluorooctanoic Acid in the Rivers of Tokyo     

Y. Takazawa  T. Nishino  Y. Sasaki  H. Yamashita  N. Suzuki  K. Tanabe  Y. Shibata 《Water, air, and soil pollution》2009,202(1-4):57-67

Comprehensive survey of major rivers in the Tokyo metropolitan area was conducted for clarifying the emission sources of perfluorooctane sulfonate (PFOS) in Tokyo. PFOS was found at all sampling sites at concentrations ranging from 0.5 to 58 ng L^?1; in addition to this, it was also indicated that unknown PFOS emission sources are present in the midstream of the Tama River basin. The relationship between PFOS and perfluorooctanoic acid (PFOA) was constant at a ratio of 10:3 (PFOS/PFOA) throughout the Tama River basin. The sum of daily load amounts of PFOS from Tokyo’s major rivers to Tokyo Bay reached 215 g day^?1. This value corresponds to 12.8 μg day^?1 per person using the sum of wastewater treatment district populations. In contrast, an estimation of PFOS contribution of domestic wastewater was also attempted, and the contribution was 1.6 μg day^?1 per person. We took samples up trunk sewers in the Tama River and finally found at the highest PFOS concentration (58,000 ng L^?1) from one of the wastewater of the electronic parts manufacturing facilities.  相似文献   

Comparison of extracapillary and endocapillary blood flow oxygenators for open heart surgery in dogs: efficiency of gas exchange and platelet conservation     

Hoshi K  Tanaka R  Shibazaki A  Nagashima Y  Hirao H  Namiki R  Takashima K  Noishiki Y  Yamane Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(3):357-361

The goal of the current study was to compare the efficiency of gas exchange and platelet conservation of a new extracapillary blood flow oxygenator versus an endocapillary blood flow oxygenator during open heart surgery with extracorporeal circulation in dogs. Dilation and remodeling of the right ventricular outflow tract of dogs was performed using a patch graft technique to simulate pulmonary stenosis. Sequential pre- and post-operative blood analysis revealed that gas exchange efficiency and platelet conservation was significantly greater with the extracapillary blood flow oxygenator than with the endocapillary blood flow oxygenator. However, the priming volume of the extracapillary blood flow oxygenator was significantly greater, leading to hemodilution. We conclude that while the extracapillary blood flow oxygenator provided benefits in terms of gas exchange and platelet conservation, development of a smaller extracapillary blood flow type oxygenator to reduce hemodilution effects would be beneficial.  相似文献   

Regulation of uterine function by cytokines in cows: Possible actions of tumor necrosis factor-α, interleukin-1α and interferon-τ     

Kiyoshi OKUDA  Ryosuke SAKUMOTO 《Animal Science Journal》2006,77(3):266-274

When animals do not become pregnant, regression of the corpus luteum (CL) is essential for normal cyclicity because it allows the development of a new ovulatory follicle. Luteal regression is caused by a pulsatile release of prostaglandin (PG) F_2α from the uterus in the late luteal phase in most mammals including cattle. Although it has been proposed in ruminants that pulsatile PGF_2α secretion is generated by a positive feedback loop between luteal and/or hypophyseal oxytocin and uterine PGF_2α, the bovine endometrium may possess other mechanisms for initiation of luteolytic PGF_2α secretion. There is increasing evidence that several cytokines mainly produced by immune cells modulate CL and uterine function in many species. Tumor necrosis factor‐α (TNF‐α) stimulates PGF_2α output from bovine endometrium not only at the follicular phase but also at the late luteal phase. Administration of TNF‐α at a high concentration prolongs luteal lifespan, whereas administration of a low concentration of TNF‐α accelerates luteal regression in cows. The data obtained from the authors’ previous in vitro and in vivo studies strongly suggest that TNF‐α is a crucial factor in regulating luteolysis in cows. The authors’ recent study has shown that interleukin‐1α mediates PG secretion from bovine endometrium as a local regulator. Furthermore, interferon‐τ (IFN‐τ) suppresses the action of TNF‐α on PGF_2α synthesis by the bovine endometrium in vitro, suggesting that IFN‐τ plays a luteoprotective role by inhibiting TNF‐α‐induced PGF_2α production in early pregnancy. The purpose of the present review is to summarize current understanding of the endocrine mechanisms that regulate uterine function by cytokines during the estrous cycle and early pregnancy in cows.  相似文献   

Dielectric relaxation due to heterogeneous structure in moist wood     

Hiroyuki Sugimoto  Ryosuke Takazawa  Misato Norimoto 《Journal of Wood Science》2005,51(6):549-553

Dielectric properties in three main directions for hinoki wood (Chamaecyparis obtusa) specimens conditioned at various levels of relative humidity were measured in the frequency range from 20 Hz to 10 MHz over the temperature range from −150°C to 20°C. Three relaxations were observed in the specimens conditioned at high levels of relative humidity. The relaxation in the highest frequency range was ascribed to the motions of adsorbed water molecules. The relaxation in the middle frequency range remained unchanged by the ethanol–benzene extraction of specimens. The relaxation location was independent of measuring directions. The relaxation in the lowest frequency range was not detected in the specimens impregnated with methyl methacrylate (MMA). This result suggested that the relaxation was due to electrode polarization. The Cole-Cole circular arc law applied well to two relaxations recognized in the specimens impregnated with MMA. The relaxation magnitude in the middle frequency range was extremely large, and the distribution of relaxation times was very narrow. These characteristics suggested relaxation of the Maxwell-Wagner type resulting from the interfacial polarization in the heterogeneous structure, which included adsorbed water with large electrical conductivity within the insulating cell walls.  相似文献   

Expression of matrix metalloproteinases in bovine luteal cells induced by prostaglandin F2α, interferon γ and tumor necrosis factor α     

Hironori ABE  Ryosuke SAKUMOTO  Kiyoshi OKUDA 《The Journal of reproduction and development》2015,61(4):277-286

We recently demonstrated that luteal cells flow out from the ovary via lymphatic vessels during luteolysis. However, the regulatory mechanisms of the outflow of luteal cells are not known. Matrix metalloproteinases (MMPs) can degrade the extracellular matrix and basal membrane, and tissue inhibitors of matrix metalloproteinases (TIMPs) inhibit the activity of MMPs. To test the hypothesis that MMP expression in luteal cells is regulated by luteolytic factors, we investigated the effects of prostaglandin F2α (PGF), interferon γ (IFNG) and tumor necrosis factor α (TNF) on the mRNA expression of MMPs and TIMPs in cultured luteal cells. Luteal cells obtained from the CL at the mid-luteal stage (days 8–12 after ovulation) were cultured with PGF (0.01, 0.1, 1 μM), IFNG (0.05, 0.5, 5 nM) and TNF (0.05, 0.5, 0.5 nM) alone or in combination for 24 h. PGF and IFNG significantly increased the expression of MMP-1 mRNA. In addition, 1 μM PGF in combination with 5 nM IFNG stimulated MMP-1 and MMP-9 mRNA expression significantly more than either treatment alone. In contrast, IFNG significantly decreased the level of MMP-14 mRNA. The mRNA expression of TIMP-1, which preferentially inhibits MMP-1, was suppressed by 5 nM INFG. One μM PGF and 5 nM IFNG suppressed TIMP-2 mRNA expression. These results suggest a new role of MMPs: luteal MMPs stimulated by PGF and IFNG break down the extracellular matrix surrounding luteal cells, which accelerates detachment from the CL during luteolysis, providing an essential prerequisite for outflow of luteal cells from the CL to lymphatic vessels.  相似文献   

Comparison of the global gene expression profiles in the bovine endometrium between summer and autumn     

Ryosuke SAKUMOTO  Ken-Go HAYASHI  Shiori SAITO  Hiroko KANAHARA  Keiichiro KIZAKI  Kosuke IGA 《The Journal of reproduction and development》2015,61(4):297-303

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Estimation of Glomerular Filtration Rate in Cynomolgus Monkeys (Macaca fascicularis)     

Ryosuke IWAMA  Tsubasa SATO  Ken SAKURAI  Kiyoshi TAKASUNA  Toshihiro ICHIJO  Kazuhisa FURUHAMA  Hiroshi SATOH 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(10):1423-1426

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Follicular Thyroid Carcinoma Characterized by Abundant Stromal Components with Chondroid and Osseous Metaplasia in a Dog     

Ryosuke KOBAYASHI  Naoaki YAMADA  Takashi KITAMORI  Fumiyo KITAMORI  Kazunari SATO  Takuya DOI  Yumi WAKO  Junko SATO  Minoru TSUCHITANI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(8):1161-1164

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