The F38-Like Group,a New Group of Caprine Mycoplasmas?     

H. Ern   R. H. Leach  M. M. Salih  K. J. MacOwan 《Acta veterinaria Scandinavica》1983,24(3):275-286

This paper concerns the taxonomic status of the F38-like group (MacOwan), a prime determinant of contagious caprine pleuropneumonia (CCPP). Extensive biochemical and serological investigations on strain F38 are reported. Some complex serological relationships with other mycoplasma species are revealed. The results, taken in conjunction with earlier published work on geno-typic characters, lead to the conclusion that final classification of these organisms should await further comparative studies of a number of field strains with a related group of strains classified as M. capri-colum.The characterization of F38 confirms its partial relationship to the “M. mycoides group” of ovine/caprine/bovine mycoplasmas, and has also revealed a very close phenotypic relationship to the bovine mycoplasma serogroup 7, a finding of potential diagnostic and epidemiological importance.Key words: mycoplasmas, classification, F38-like group  相似文献   

Determination of vc and mating types of Cryphonectria parasitica isolates by multiplex PCR and their genetic diversity in 13 chestnut-growing provinces of Turkey     

Deniz Çakar  Göksel Özer  Seçil Akıllı Şimşek  Salih Maden 《Forest Pathology》2023,53(3):e12813

Vegetative compatibility (vc) and mating types and genetic diversity of Cryphonectria parasitica isolates were determined using 183 isolates obtained from 215 infected chestnut trees growing in 13 provinces of Turkey. Based on the cultural aspects, 143 of these isolates were evaluated as virulent whereas the remaining 40 isolates were hypovirulent. When vc types of 183 isolates were classically differentiated, 135 of them matched to EU-1 (82.3%), 29 of them to EU-12 (17.6%) vc type, whereas 19 of them did not match to the two. When molecular vic markers were used, all the isolates were assigned to two EU vc types; 149 to EU-1 (81.4%) and 34 (18.5%) to EU-12. Of the majority of the isolates, 134 (73.2%) had mating-type MAT-1, while 44 (24%) isolates had MAT-2 and 5 (2.8%) isolates had both mating types. The population analysis based on two DNA marker systems, Inter-Primer Binding Site and Start Codon Targeted Polymorphism, showed no intraspecific genetic variation among the C. parasitica isolates. The prevalence of two dominant vc types revealed by this study shows that biological control with hypovirulent EU-1 and EU-12 isolates will be significant for the country. The results might be helpful to chestnut breeders carrying out resistance breeding studies to manage this disease based on hypovirulence attributed to Cryphonectria hypovirus 1.  相似文献   

Investigation of biochemical and haematological side-effects of cefquinome in healthy dogs     

Maden M  Traş B  Baş AL  Elmas M  Yazar E  Birdane FM 《The Veterinary quarterly》2001,23(1):32-34

In the present study, the effects of cefquinome, a 4th generation cephalosporin, on clinical, biochemical, haematological, and blood gas variables were investigated. Five healthy dogs were injected with cefquinome (1 mg/kg body weight, IM, daily) for 14 days. Negative effects of cefquinome on clinical, biochemical, and haematological variables were not observed, but it did change some blood gas variables.  相似文献   

Miliary bovine farcy experimentally induced in a Zebu calf.     

S M El Sanousi  M A Salih 《Veterinary pathology》1979,16(3):372-373

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Identification and localization of a 94 kDa membrane protein found in Mycoplasma bovoculi strains     

Barik A Salih  Ricardo F Rosenbusch 《Comparative immunology, microbiology and infectious diseases》1998,21(4):281-290

Six isolates of Mycoplasma bovoculi obtained from cattle herds with bovine keratoconjunctivitis were analyzed by gel electrophoresis and immunoblotting techniques. All six strains showed similarity in their protein profiles although no two patterns were identical. Antigenic differences between strains were detected in immunoblots reacted with post-exposure calf serum. A common 94 kDa protein band designated p94 was detected in all six strains reacted with monoclonal antibody MA25.5 developed to one of the strains. The p94 was also recognized in these strains by the calf serum. Trypsin treatment of intact mycoplasma cells resulted in the removal of p94 from immunoblots reacted with MA or hyperimmune rabbit serum. Other trypsin-resistant antigens shared between strains or being strain-specific in nature were identified when trypsin-treated mycoplasma cells were reacted with hyperimmune rabbit serum. The p94 antigen was shown to be of mycoplasmal origin by radio-immunoprecipitation using the MA or hyperimmune rabbit serum. These studies identify the presence of a surface antigen (p94) on M. bovoculi membrane in all strains examined that is trypsin sensitive by the use of monoclonal antibody, calf serum and hyperimmune rabbit serum.

Résumé

Six souches de Mycoplasma bovoculi, d'origine bovine (animaux affectés de keratoconjunctivite) ont été analysées par des techniques d'éléctrophorèse et par ‘immunoblotting’. Les six souches ont montré des similitudes dans leurs profils proteiniques. Cependant, aucun profil n'a été identique. Des differences antigéniques entre les souches ont étés detectées suite au traitement des immunoblots avec un sérum bovin infecté par M. bovoculi. L'anticorps monoclonal MA25.5 dirigé contre une des souches a réagi avec une bande proteinique (94 kDa) commune aux six souches. Cette bande a été designee p94. Cette bande p94 a été également identifiée chez tous les souches en utilisant le sérum bovin. Le traitement des mycoplasmes intactes par la trypsine a éliminé la bande p94 des immunoblots: cela a été démontr é par l'usage d'un anticorps monoclonal, ou par un sérum hyperimmun de lapin. D'autres antigènes résistants à la trypsine, communs ou spécifiques aux souches ont été identifiés quand les mycoplasmes traités par le trypsine ont été mélangés au sérum hyperimmun de lapin. La radioimmunoprécipitation par l'anticorps monoclonal ou le sérum hyperimmun de lapin a demontré que l'antigène p94 est d'origine mycoplasmique. Ces études identifient la présence d'un antigène de surface (le p94) localisé sur la membranc de M. bovoculi chez toutes les souches examinées. Cet antigène est sensible à la trypsine. Cela a été montré avec l'anticorps monoclonal, le sérum bovin et le sérum hyperimmune du lapin.  相似文献   

Bladder Eversion Caused by Chronic Cystitis in an Arabian Racehorse: A Case Report     

Cihan Kumas  Mehmet Maden 《Journal of Equine Veterinary Science》2014

Bladder eversion is a rare condition and may occur in mares as a result of excessive straining during pregnancy or in the postpartum period. In the present case, bladder eversion was caused by chronic cystitis in a nonpregnant mare. An Arabian racehorse (mare, 3 years old) was admitted to The Racehorse Hospital of the Turkish Jockey Club with a history of lumbar pain, excessive straining, and frequently assuming the urination position. Physical examination revealed the presence of tenesmus, stranguria, passing of small amounts of urine, and a visible mucosal structure at the ventral vulvar commissure during tenesmus. Laboratory findings revealed leucocytosis, increased urine pH, proteinuria, pyuria, and hematuria. Streptococcus equi subsp. zooepidemicus and Escherichia coli were isolated and identified in urine culture. Transrectal ultrasonography revealed thickening of the bladder wall and prolapse of the bladder corpus into the bladder. In the cystoscopic examination, performed following bladder reduction, severe hyperemia, erosion, and ulcers were determined in the bladder mucosa. Chronic cystitis was treated using antibiotics, based on urine culture test results, together with steroids and non-steroidal anti-inflammatory drugs. Twenty-four hours after the start of treatment, the severity of straining was observed to have decreased, ceasing completely on Day 4, and the bladder returned to its normal position.  相似文献   

Soil Salinization in Some Irrigated Areas of the Kingdom of Bahrain     

Asma Ali Abahussain  Abdelhadi Abdelwahab Mohamed  Ahmed Ali Salih  Ahmad Al Safe  Nader Abdul Hamed Mosa  Yahya Othman 《农业科学与技术》2014,(2):112-122

Monitoring and assessment of agricultural land degradation is of vital importance for better land and water management planning and reclamation. It requires setting baseline information and basic analysis at specific time and space. About 33 geo-referenced soil sampling spots were selected in two agricultural production locations in the Kingdom of Bahrain to assess the status and preliminary causes of land degradation. Soil samples were taken from 13 sites in Diraz location while 19 samples were taken from Budayyi location. The samples were taken to 90 cm depth at 30 cm intervals. Standard procedures were followed to determine soil physiochemical properties. In addition, field observations on farm condition, distance from the sea, method of irrigation and irrigation water source were taken. Some of the soil samples were deliberately taken from outside the irrigated basins among trees compared with samples taken from inside the actively growing area for comparison. The results indicated that the salinity level was significantly （P 〈 0.001） higher at the 0-30 cm soil depth compared with 30-60 cm or 60-90 cm depths in both locations. The distance from the sea did not show clear correlation with surface soil salinity in Budayyi area compared with Diraz. Both locations showed significantly higher salinity levels on samples taken outside the actively growing areas compared with those taken from within. The effect is more prominent at the 0-30 cm depth. The observed variability on salinity levels may be attributed to farm management practices and deteriorating quality of ground water. Thus, agricultural land degradation in Bahrain cannot be attributed to ground water deterioration alone. The use of tertiary treated sewage water （TSE） may ease the pressure on ground water, but the pH of the TSE should be carefully monitored and managed with proper studies on leaching requirements to avoid further salinity complications.  相似文献   

Mutations in a human ROBO gene disrupt hindbrain axon pathway crossing and morphogenesis     

Jen JC  Chan WM  Bosley TM  Wan J  Carr JR  Rüb U  Shattuck D  Salamon G  Kudo LC  Ou J  Lin DD  Salih MA  Kansu T  Al Dhalaan H  Al Zayed Z  MacDonald DB  Stigsby B  Plaitakis A  Dretakis EK  Gottlob I  Pieh C  Traboulsi EI  Wang Q  Wang L  Andrews C  Yamada K  Demer JL  Karim S  Alger JR  Geschwind DH  Deller T  Sicotte NL  Nelson SF  Baloh RW  Engle EC 《Science (New York, N.Y.)》2004,304(5676):1509-1513

The mechanisms controlling axon guidance are of fundamental importance in understanding brain development. Growing corticospinal and somatosensory axons cross the midline in the medulla to reach their targets and thus form the basis of contralateral motor control and sensory input. The motor and sensory projections appeared uncrossed in patients with horizontal gaze palsy with progressive scoliosis (HGPPS). In patients affected with HGPPS, we identified mutations in the ROBO3 gene, which shares homology with roundabout genes important in axon guidance in developing Drosophila, zebrafish, and mouse. Like its murine homolog Rig1/Robo3, but unlike other Robo proteins, ROBO3 is required for hindbrain axon midline crossing.  相似文献   

Quality of Tigris River passing through Baghdad for irrigation     

S. M. Mutlak  B. M. Salih  S. J. Tawfiq 《Water, air, and soil pollution》1980,13(1):9-16

The effect of Baghdad city on the water quality of the Tigris River was studied from April 1977 to March 1978. The chemical and physical characteristics of the water which are necessary in judging the quality of water for irrigation were studied. It was found that Baghdad was responsible for increasing the water salinity from 390 to 443 mg l^?1. Total hardness and turbidity were increased when the river passed through Baghdad. The increase in the total hardness was mostly due to the increase in Mg concentration. The results also suggested that there should be no problem from the heavy metals or inorganic N in this water when used for irrigation. The Tigris River water in Baghdad was classified as classC ₂ S ₁; that is water of second class with regard to salinity and first class with regard to sodicity.  相似文献   

In vitro hatching of the infective eggs of Ascaridia galli in tissue extracts     

N E Salih  K M Saleem 《Veterinary parasitology》1987,24(3-4):263-268

Extracts of proventriculus (PE) and small intestine (IE) of fowl were used, for the first time, as media for the in vitro hatching of infective eggs of Ascaridia galli. Hatching was successful in a mixture of equal parts of PE and IE (MIP) under normal air and under different concentrations of CO2, but not under 100% CO2. A very highly significant retardation (P less than 0.001) in the rate of hatching appeared as the concentration of CO2 was increased, indicating that high levels of CO2 inhibit the process of hatching. 100% of the eggs hatched in PE in 40.5 min whereas they did not hatch at all in IE, suggesting that IE alone has no influence on egg hatching. The shortest time taken for 100% of the eggs to hatch in this study suggests that the present method of hatching is faster than all previous methods used by different workers.  相似文献