Effects of land use on infestation and parasitism rates of cabbage seed weevil in oilseed rape     

Gabriella Kovcs  Riina Kaasik  Marjolein E Lof  Wopke van der Werf  Tanel Kaart  John M Holland  Anne Luik  Eve Veromann 《Pest management science》2019,75(3):658-666

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Potential of parasitoids to control populations of the weevil Ceutorhynchus obstrictus (Marsham) on winter oilseed rape     

E. Veromann  I. H. Williams  R. Kaasik  A. Luik 《国际虫害防治杂志》2013,59(1):85-92

Ceutorhynchus obstrictus (Marsham) (Coleoptera: Curculionidae) is a major pest of oilseed rape in Europe and North America. The last decade has seen growing interest in the occurrence, importance and efficiacy of its parasitoids as biocontrol agents. The infestation of pods by C. obstrictus larvae and their parasitism rates were assessed in a total of nine commercially-grown crops and fifteen experimental plots of winter oilseed rape in 2004–2007. Pod samples were collected at the pod maturation stage and incubated in emergence traps. Emerged larvae, their exit holes and parasitoids were counted and identified; the percentage of infested pods and parasitism rates were calculated. Pod infestation by larvae increased continuously over the study period. The parasitism level was lowest in 2005 and increased thereafter up to 96%. The most abundant parasitoid was Trichomalus perfectus (Walker), but Mesopolobus morys (Walker) and Stenomalina gracilis (Walker) (Hymenoptera: Pteromalidae) were also reared from host larvae. This study showed that naturally-occurring ectoparasitoids of C. obstrictus were capable of becoming established as viable populations and have the potential to contribute to biological control of C. obstrictus.  相似文献   

Towards dsRNA-integrated protection of medical Cannabis crops: considering human safety,recent- and developing RNAi methods,and research inroads     

Jonathan Willow  Ana I. Silva  Clauvis Nji Tizi Taning  Guy Smagghe  Eve Veromann 《Pest management science》2023,79(4):1267-1272

Owing to the expanding industry of medical Cannabis, we discuss recent milestones in RNA interference (RNAi)-based crop protection research and development that are transferable to medical Cannabis cultivation. Recent and prospective increases in pest pressure in both indoor and outdoor Cannabis production systems, and the need for effective nonchemical pest control technologies (particularly crucial in the context of cultivating plants for medical purposes), are discussed. We support the idea that developing RNAi tactics towards protection of medical Cannabis could play a major role in maximizing success in this continuously expanding industry. However, there remain critical knowledge gaps, especially with regard to RNA pesticide biosafety from a human toxicological viewpoint, as a result of the medical context of Cannabis product use. Furthermore, efforts are needed to optimize transformation and micropropagation of Cannabis plants, examine cutting edge RNAi techniques for various Cannabis–pest scenarios, and investigate the combined application of RNAi- and biological control tactics in medical Cannabis cultivation. © 2022 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.  相似文献   

Targeting a coatomer protein complex-I gene via RNA interference results in effective lethality in the pollen beetle Brassicogethes aeneus     

Willow  Jonathan  Sulg  Silva  Taning  Clauvis Nji Tizi  Silva  Ana Isabel  Christiaens  Olivier  Kaasik  Riina  Prentice  Katterinne  Lövei  Gabor L.  Smagghe  Guy  Veromann  Eve 《Journal of pest science》2021,94(3):703-712

The pollen beetle Brassicogethes aeneus is a serious pest of oilseed rape (Brassica napus) in Europe. Management of this pest has grown difficult due to B. aeneus’s development of resistance to pyrethroid insecticides, as well as the pressure to establish control strategies that minimise the impact on nontarget organisms. RNA interference represents a nucleotide sequence-based, and thus potentially species-specific, approach to agricultural pest control. The present study examined the efficacy of targeting the coatomer gene coatomer subunit alpha (αCOP), via both microinjection and dietary exposure to exogenous complementary dsRNA, on αCOP-silencing and subsequent mortality in B. aeneus. Beetles injected with dsRNA targeting αCOP (at 0.14 µg/mg) showed 88% and 100% mortality at 6 and 10 days post-injection, respectively; where by the same time after dietary exposure, 43%–89% mortality was observed in the 3 µg dsRNA/µL treatment, though the effect was concentration-dependent. Thus, the effect was significant for both delivery routes. In working towards RNA-based management of B. aeneus, future studies should include αCOP as a target of interest.

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