Accuracy of imputation of single nucleotide polymorphism marker genotypes from low‐density panels in Japanese Black cattle          下载免费PDF全文

Shinichiro Ogawa  Hirokazu Matsuda  Yukio Taniguchi  Toshio Watanabe  Akiko Takasuga  Yoshikazu Sugimoto  Hiroaki Iwaisaki 《Animal Science Journal》2016,87(1):3-12

Using target and reference fattened steer populations, the performance of genotype imputation using lower‐density marker panels in Japanese Black cattle was evaluated. Population imputation was performed using BEAGLE software. Genotype information for approximately 40 000 single nucleotide polymorphism (SNP) markers by Illumina BovineSNP50 BeadChip was available, and imputation accuracy was assessed based on the average concordance rates of the genotypes, varying equally spaced SNP densities, and the number of individuals in the reference population. Two additional statistics were also calculated as indicators of imputation performance. The concordance rates tended to be lower for SNPs with greater minor allele frequencies, or those located near the ends of the chromosomes. Longer autosomes yielded greater imputation accuracies than shorter ones. When SNPs were selected based on linkage disequilibrium information, relative imputation accuracy was slightly improved. When 3000 and 10 000 equally spaced SNPs were used, the imputation accuracies were greater than 90% and approximately 97%, respectively. These results indicate that combining genotyping using a lower‐density SNP chip with genotype imputation based on a population of individuals genotyped using a higher‐density SNP chip is a cost‐effective and valid approach for genomic prediction.  相似文献   

Effect of Pimobendan in Dogs with Preclinical Myxomatous Mitral Valve Disease and Cardiomegaly: The EPIC Study—A Randomized Clinical Trial          下载免费PDF全文

A. Boswood  J. Häggström  S.G. Gordon  G. Wess  R.L. Stepien  M.A. Oyama  B.W. Keene  J. Bonagura  K.A. MacDonald  M. Patteson  S. Smith  P.R. Fox  K. Sanderson  R. Woolley  V. Szatmári  P. Menaut  W.M. Church  M. L. O'Sullivan  J.‐P. Jaudon  J.‐G. Kresken  J. Rush  K.A. Barrett  S.L. Rosenthal  A.B. Saunders  I. Ljungvall  M. Deinert  E. Bomassi  A.H. Estrada  M.J. Fernandez Del Palacio  N.S. Moise  J.A. Abbott  Y. Fujii  A. Spier  M.W. Luethy  R.A. Santilli  M. Uechi  A. Tidholm  P. Watson 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2016,30(6):1765-1779

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The organic acid profiles in the feces of pigs at Brachyspira hyodysenteriae- or B. pilosicoli-positive farms     

Piao SJ  Tsukahara T  Itoh M  Shiga A  Adachi Y  Ushida K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(4):425-428

We observed a significant difference in the organic acid profile of diarrheal feces between pigs infected with and free from pathogenic spirochetes. Diarrhea and loose feces were collected from growing pigs, held at 15 different commercial farms. A total of 106 samples were measured for organic acid concentration by HPLC and were checked for the presence of B. hyodysenteriae and B. pilosicoli by PCR. B. hyodysenteriae was detected in 3 samples collected from one farm. B. pilosicoli was detected in 5 samples collected from another farm. Lower concentrations of iso-butyrate and iso-valerate were likely associated with development of pathogenic spirochete infection.  相似文献   

Rice cultivation and its environmental conditions in the mediterranean countries I. Climate and physiography in relation to rice cultivation     

Yoshikazu Takaya  Kazutake Kyuma  Keizaburo Kawaguchi 《Soil Science and Plant Nutrition》2013,59(3):209-223

In order to characterize the rice cultivation in the Mediterranean countries, climate and physiography of rice growing areas in Italy, Spain, and Portugal were studied. In northern Italy rice is extensively cultivated on terraces and fluvial plains of the Po and its tributaries, making use of the supply of water from Alpine glaciers in addition to the relatively abundant rainfalls of the area. In the Iberian peninsula summer climate is so dry that rice is cultivable only where the ratio of catchment area to rice land area is sufficiently large, thus rice lands are practically limited to the floodplains and deltas of the big rivers, such as the Sado and the Tejo in Portugal and the Guadalquivir and the Ebro in Spain.

In order to characterize the rice cultivation in the Mediterranean countries, climate and physiography of rice growing areas in Italy, Spain, and Portugal were studied. In northern Italy rice is extensively cultivated on terraces and fluvial plains of the Po and its tributaries, making use of the supply of water from Alpine glaciers in addition to the relatively abundant rainfalls of the area. In the Iberian peninsula summer climate is so dry that rice is cultivable only where the ratio of catchment area to rice land area is sufficiently large, thus rice lands are practically limited to the floodplains and deltas of the big rivers, such as the Sado and the Tejo in Portugal and the Guadalquivir and the Ebro in Spain.  相似文献   

Long‐term field insecticide susceptibility data and laboratory experiments reveal evidence for cross resistance to other neonicotinoids in the imidacloprid‐resistant brown planthopper Nilaparvata lugens     

Tomohisa Fujii  Sachiyo Sanada‐Morimura  Takaho Oe  Manami Ide  Dinh Van Thanh  Ho Van Chien  Phan Van Tuong  Phung Minh Loc  Le Quoc Cuong  Ze‐Wen Liu  Zeng‐Rong Zhu  Jian‐Hong Li  Gang Wu  Shou‐Horng Huang  Gerardo F Estoy  Shoji Sonoda  Masaya Matsumura 《Pest management science》2020,76(2):480-486

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Iron uptake in a bicarbonate-resistant cell line of tobacco     

Yoshikazu Kiriiwa  Sunao Osumi  Masanobu Endo  Akira Nukaya  Akio Morita  Hiromi Yokota 《Soil Science and Plant Nutrition》2013,59(7):1119-1124

Abstract

In alkaline soils, plant growth is impaired mainly by high pH and high concentration of bicarbonates. The bicarbonate concentration increases the pH value, and causes deficiency of iron. A bicarbonate-resistant cell line (BR line) of tobacco (Nicotiana tabacum L. cv. Burley21) was selected by adding excess bicarbonate ions (20 mmol L^?1) to the culture medium. The pH of the medium was buffered 8.0 to 8.3. Under these conditions, about 80% of iron in the medium became insoluble. However, under such conditions, the BR line grew well. In this report, we examined some characteristics of the growth and iron uptake in the BR line under iron-deficient (i.e. high pH or no-iron) condition. At pH 5.8, the Fe³⁺ reduction activity was not significantly different between the non-selected line and the BR line. At pH 8.0, however, the Fe³⁺ reduction activity of the BR line was higher than that of the non-selected line. In no-iron condition, the growth of the non-selected line was markedly reduced after 2 weeks, while the BR line was not affected. The content of malic acid in both lines increased with the medium pH, and the content in the BR line was higher than that in the non-selected line. The BR line was able to adapt to the conditions, which restricted iron uptake, such as high bicarbonate concentration, high pH, and low iron conditions. The high ability of Fe³⁺ reduction was maintained at even high pH conditions. Further, the BR line may be able to improve the utilization of iron in the cells.  相似文献   

Therapeutic effect of anti-TNF-alpha antibody and levofloxacin (LVFX) in a mouse model of enterohemorrhagic Escherichia coli O157 infection     

Isogai E  Isogai H  Hirose K  Kubota T  Kimura K  Fujii N  Hayashi S  Takeshi K  Oguma K 《Comparative immunology, microbiology and infectious diseases》2001,24(4):217-231

The ability of an anti-TNF-alpha antibody to confer protection against enterohaemorrhagic Escherichia coli (EHEC) O157 was investigated in germfree IQI mice. The use of an antibiotic levofloxacin (LVFX) alone or with the antibody was also studied. Protection included an increase in survival rate. Treatment with the anti-TNF-alpha antibody inhibited the histological signs associated with EHEC infection but did not prevent the colonization of EHEC or production of Shiga toxin (Stx). No clinical signs were observed and EHEC was completely eliminated in the mouse model receiving both anti-TNF-alpha antibody and LVFX. Anti-TNF-alpha antibody suppressed inflammatory cytokine response in the mouse kidney and brain by EHEC infection.  相似文献   

Silicon content in rice seedlings to protect rice blast fungus at the nursery stage   总被引：1，自引：0，他引：1  

Tsuyoshi Hayasaka  Hiroshi Fujii  Tsuneo Namai 《Journal of General Plant Pathology》2005,71(3):169-173

To control rice blast effectively at the nursery stage, the absolute SiO₂ content necessary for rice plants to resist blast disease was investigated using various rice cultivars and soils. Nine rice cultivars with different complete resistance genes and different degrees of partial resistance were grown on nursery soils amended with silica gel at different rates to change the SiO₂ content of rice plant. The rice seedlings were then inoculated 28 days after sowing with Pyricularia grisea to estimate their blast resistance. In all rice cultivars, the number of lesions was significantly reduced when SiO₂ content increased in the rice seedling; lesions were reduced to 5%–20% of the number on the seedlings grown in soil without silica gel when the seedling SiO₂ content reached 5%. Additionally, the susceptibility to blast disease of rice seedlings grown on eight soils collected from different districts, with varying amounts of silica gel, was compared. The number of lesions decreased significantly when the SiO₂ content in the seedlings reached 5%. These results suggest that SiO₂ content of at least 5% in the rice plant can control this disease at the nursery stage under any conditions.  相似文献   

Evaluation of doping drugs by treadmill exercise in dogs. I. Caffeine (author's transl)     

C Kusanagi  S Fujii  S Inada 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1974,36(2):81-92

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Pharmacological studies on doping drugs for race horses. IV. Chlorpromazine and phenobarbital (author's transl)]     

S Fujii  S Inada  S Yoshida  C Kusanagi  K Mima 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1975,37(2):133-139

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