Clinical studies on bovine leukemia in Japanese black cattle. III. Serum lactate dehydrogenase activity and its isoenzyme pattern in groups of leukemic cattle and those negative or positive for antibody against bovine leukemia virus     

K Ishihara  T Ohtani  H Kitagawa  M Onuma 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1980,42(3):289-295

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Green Fluorescent Detection of Fungal Colonization and Endopolygalacturonase Gene Expression in the Interaction of Alternaria citri with Citrus     

Isshiki A  Ohtani K  Kyo M  Yamamoto H  Akimitsu K 《Phytopathology》2003,93(7):768-773

ABSTRACT Alternaria citri, a postharvest pathogen, produces endopolygalacturonase (endoPG) and causes black rot on citrus fruit. We previously described that an endoPG-disrupted mutant of Alternaria citri was significantly reduced in its ability to macerate plant tissue and cause black rot symptoms on citrus. In order to investigate colonization of citrus fruit tissues by Alternaria citri, pTEFEGFP carrying a green fluorescent protein (GFP) gene was introduced into wild-type Alternaria citri and its endoPG-disrupted mutant (M60). Green fluorescence was observed in spores, germ tubes, appressoria, and infection hyphae of transformants G1 (derived from wild type) and GM4 (derived from M60). Hyphae of G1 but not GM4 vertically penetrated the peel, but the hyphae of both G1 and GM4 spread equally in the juice sac area of citrus fruit. Green fluorescence of Alternaria citri transformant EPG7 carrying a GFP gene under control of the endoPG gene promoter of Alternaria citri was induced by pectin in the peel during the infection stage, but repressed completely in the juice sac area, likely by carbon catabolite repression by sugars in the juice.  相似文献   

Spectral analysis of heart rate variability in dogs with mild mitral regurgitation     

Fujii Y  Wakao Y 《American journal of veterinary research》2003,64(2):145-148

OBJECTIVE: To assess autonomic function in dogs with mild mitral regurgitation (MR) that did not have clinical signs of the condition. ANIMALS: 6 healthy adult Beagles. PROCEDURE: Mild MR was experimentally induced. A 24-hour ambulatory ECG was recorded before and after induction of MR. Heart rate variability was analyzed in frequency domains by use of the ambulatory ECG. Low-frequency (LF) and high-frequency (HF) power were calculated by integrating over their frequency intervals, and the ratio of LF to HF was also calculated. Measurements of frequency domains were analyzed for 4 time periods (midnight to 6 AM, 6 AM to noon, noon to 6 PM, and 6 PM to midnight). RESULTS: Dogs with experimentally induced MR were classified as International Small Animal Cardiac Health Council class Ia. The HF power of dogs with MR was significantly decreased between 6 AM and noon. The ratio of LF to HF in dogs with MR was significantly increased for the periods between midnight and 6 AM, 6 AM and noon, and noon and 6 PM. CONCLUSIONS AND CLINICAL RELEVANCE: Compensatory response through autonomic modulation was observed in dogs with mild MR that did not have abnormalities, except for cardiac murmur, during clinical examination. This result suggests that treatment during the early stages of mild MR may be beneficial. Additional studies are necessary to determine whether such treatment will delay the onset of congestive heart failure and prolong survival in dogs affected with mild MR.  相似文献   

Increase of Th1 type cytokine mRNA expression in peripheral blood lymphocytes of calves experimentally infected with Cryptosporidium parvum     

Takeda K  Omata Y  Koyama T  Ohtani M  Kobayashi Y  Furuoka H  Matsui T  Maeda R  Saito A 《Veterinary parasitology》2003,113(3-4):327-331

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Automatic detection of washboarding in bandsaws     

Nanfeng Zhu  Chiaki Tanaka  Tadashi Ohtani  Hiroshi Usuki 《Journal of Wood Science》2001,47(2):102-108

This paper presents an on-line method for detecting washboarding generated in bandsaws. It uses a parameter based on the shape of sawing force amplitude distributions. The curve of the probability density function of the parallel sawing force amplitude obeyed a normal distribution for the normal sawn surface, but it changed to an M-shaped distribution when washboarding appeared on the sawn surface of the workpiece. The curve of probability density function of the normal sawing force amplitude obeyed the normal distribution for any sawn surface investigated. The workpiece including a knot did not influence use of the parameter for detecting the appearance of washboarding.  相似文献   

Locating and identifying sound knots and dead knots on sugi by the rule-based color vision system     

Chuanshuang?Hu  Chiaki?TanakaEmail author  Tadashi?Ohtani 《Journal of Wood Science》2004,50(2):115-122

Knowledge of the occurrence of sound and dead knots on the surface of sugi is important for the classification and application of the material. This study examined a color vision system for detecting sound and dead knots on sugi. The system can be conceptually divided into three components: a CCD-camera scanning system, an image-segmenting module, and a rule-based defect identifying module. The results showed that the potential defect regions could be located by Otsus threshold algorithm in conjunction with t-test analysis. The accuracies of locating sound knots and dead knots were 92.6% and 97.1%, respectively. The rule-based approach was used to identify sound and dead knots and the identifying accuracies for sound knots and dead knots were 92.0% and 94.1%, respectively. The overall detection accuracy of the system was 87.6%. The results indicated that the rule-based color vision system is an efficient means of detecting sound knots and dead knots on sugi.  相似文献   

Prolonging hypothermic storage (4 C) of bovine embryos with fish antifreeze protein     

Atsushi IDETA  Yoshito AOYAGI  Kanami TSUCHIYA  Yuuki NAKAMURA  Kou HAYAMA  Atsushi SHIRASAWA  Kenichiro SAKAGUCHI  Naomi TOMINAGA  Yoshiyuki NISHIMIYA  Sakae TSUDA 《The Journal of reproduction and development》2015,61(1):1-6

Embryos obtained via superovulation are necessary for mammalian artificial reproduction, and viability is a key determinant of success. Nonfreezing storage at 4 C is possible, but currently used storage solutions can maintain embryo viability for only 24–48 h. Here we found that 10 mg/ml antifreeze protein (AFP) dissolved in culture medium 199 with 20% (v/v) fetal bovine serum and 25 mM HEPES could keep bovine embryos alive for 10 days at 4 C. We used a recombinant AFP isolated from the notched-fin eelpout (Zoarces elongatus Kner). Photomicroscopy indicated that the AFP–embryo interaction was enhanced at 37 C. Embryos pre-warmed with the AFP solution at 37 C for 60 min maintained high viability, whereas those that were not pre-warmed could live no longer than 7 days. Thus, short-term storage of bovine embryos was achieved by a combination of AFP-containing medium and controlled pre-warming.  相似文献   

Increase in plasma total antioxidant capacity of grazing Japanese Black heifers and cows in forestland in Japan     

Satoshi Haga  Hiroshi Ishizaki  Miwa Nakano  Seiji Nakao  Kiyoshi Hirano  Yoshito Yamamoto  Miya Kitagawa  Hiroyuki Sasaki  Yoshihiro Kariya 《Animal Science Journal》2014,85(2):135-142

Blood total antioxidant capacity (TAC) has become a key bio‐marker for animal health. Forest‐grazing cattle are known to forage various native plants that have high TAC. This study evaluated differences of plasma TAC between forest‐grazing (FG) and pasture‐grazing cattle (PG). Experiment 1 monitored the plasma TAC levels of 32 Japanese Black cattle. The level in PG did not change throughout the grazing period. However, that in FG, which increased from summer, was significantly higher than that in PG through fall (P < 0.05). In experiment 2, we used nine Japanese Black heifers and investigated their blood antioxidant parameters and the TAC in plants that the cattle consumed in late June and September. The plasma TAC levels in FG were significantly higher than those in PG in both periods (P < 0.05). Plasma levels of lipid peroxidation in FG tended to be lower than that in PG (P = 0.098). Furthermore, the TAC levels in various species of shrubs and trees consumed by FG were higher than those in pasture grasses. Results of this study show that plasma TAC of grazing Japanese Black cattle in forestland increase from summer through fall.  相似文献   

Detection of antigenic proteins expressed by lymphocystis virus as vaccine candidates in olive flounder, Paralichthys olivaceus (Temminck & Schlegel)     

Jang HB  Kim YR  Cha IS  Noh SW  Park SB  Ohtani M  Hikima J  Aoki T  Jung TS 《Journal of fish diseases》2011,34(7):555-562

Although the major capsid proteins (MCPs) of lymphocystis disease virus (LCDV) have been characterized, little is known about the host-derived immune response to MCPs and other LCDV antigenic proteins. To identify antigenic proteins of LCDV that could be used as vaccine candidates in olive flounder, Paralichthys olivaceus, we analysed the viral proteins responsible for its virulence by applying immuno-proteomics. LCDV proteins were separated by one-dimensional gel electrophoresis, transferred to polyvinylidene difluoride membrane, and probed with homogeneous P. olivaceus antisera elicited by LCDV natural infection and vaccination with formalin-killed LCDV. Four immune-reactive proteins were obtained at 68-, 51-, 41- and 21 kDa using antisera collected from natural infection while two proteins at 51- and 21 kDa exhibited response to antisera from vaccinated fish, indicating that the latter two proteins have vaccine potential. Using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and nanoelectrospray MS/MS, the 51 and 21 kDa proteins were identified as MCP and an unknown protein, respectively.  相似文献   

Chromosome association and pollen fertility in some interspecific hybrids of Lilium   总被引：1，自引：0，他引：1  

Yoshito Asano 《Euphytica》1982,31(1):121-128

Summary Meiotic observations in PMCs were made in eight kinds of interspecific hybrids in Lilium. Three hybrids, 6134-S × L. cernuum. L. regale × L. leichtlinii maximowiczii and L. auratum platyphyllum × L. henryi showed respectively the mean chromosome association frequencies of 1.4_II, 0.8_II and 1.5_II per cell at MI which were the lowest values hitherto reported in Lilium hybrids. In L. longiflorum × L. henryi, 33.3° of the cells had 1 or 2 multivalents of three to five chromosomes in addition to uni- and bivalents. 12° bivalents and 12 univalents were invariably observed in the triploid hybrid L. longiflorum × L. cernuum. The hybrids between the species belonging to the different sections of the genus generally showed high pollen sterility, with some exceptions. A more or less remote genomic homology was found between the different sections in Lilium.  相似文献