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Most of Pinus brutia (Ten.) Holmboe forests are grazed, as silvopastoralism is well adapted in the Mediterranean environment. However, little
attention has been paid to the demographic dynamic of the understorey vegetation even though it is strongly affected by the
absorbed radiation. The purpose of this study was to examine the adaptation of herbaceous plant species under a Pinus brutia canopy, in Northern Greece. Monocultures of four plant groups (annual and perennial grasses, annual and perennial legumes)
were sown in experimental areas of 50%, 70% and 100% light intensity. The density of annual and perennial grasses and perennial
legumes were decreased by the shade cast by the Pinus brutia canopy. Population density of the annual legumes was little affected by light intensity: the annual grasses Bromus mollis L. and Lolium rigidum Gaudin; the perennial grasses Dactylis glomerata L. var. palestine, Festuca arundinacea and Agropyron cristatum (L.) Gaertn; the perennial legume Medicago sativa L. var. romana; the annual legumes Medicago lupulina L. and all the tested varieties of Trifolium subterraneum adapted well to the 50% light intensity habitat. 相似文献
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Kassi E Papoutsi Z Fokialakis N Messari I Mitakou S Moutsatsou P 《Journal of agricultural and food chemistry》2004,52(23):6956-6961
To prevent bone loss that occurs with increasing age, nutritional and pharmacological factors are needed. Traditional therapeutic agents (selective estrogen receptor modulators or SERMs, biphosphonates, calcitonin) may have serious side effects or contraindications. In an attempt to find food components potentially acting as SERMs, we submitted four plant aqueous extracts derived from Greek flora (Sideritis euboea, Sideritis clandestina, Marticaria chamomilla, and Pimpinella anisum) in a series of in vitro biological assays reflective of SERM profile. We examined their ability (a) to stimulate the differentiation and mineralization of osteoblastic cell culture by histochemical staining for alkaline phosphatase and Alizarin Red-S staining, (b) to induce, like antiestrogens, the insulin growth factor binding protein 3 (IGFBP3) in MCF-7 breast cancer cells, and (c) to proliferate cervical adenocarcinoma (HeLa) cells by use of MTT assay. Our data reveal that all the plant extracts studied at a concentration range 10-100 microg/mL stimulate osteoblastic cell differentiation and exhibit antiestrogenic effect on breast cancer cells without proliferative effects on cervical adenocarcinoma cells. The presence of estradiol inhibited the antiestrogenic effect induced by the extracts on MCF-7 cells, suggesting an estrogen receptor-related mechanism. In conclusion, the aqueous extracts derived from Sideritis euboea, Sideritis clandestina, Marticaria chamomilla, and Pimpinella anisum may form the basis to design "functional foods" for the prevention of osteoporosis. 相似文献
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Evaluation of estrogenic/antiestrogenic activity of ellagic acid via the estrogen receptor subtypes ERalpha and ERbeta 总被引：1，自引：0，他引：1
Papoutsi Z Kassi E Tsiapara A Fokialakis N Chrousos GP Moutsatsou P 《Journal of agricultural and food chemistry》2005,53(20):7715-7720
Ellagic acid is a plant-derived polyphenol, possessing antioxidant, antiproliferative, and antiatherogenic properties. Whether this compound has estrogenic/antiestrogenic activity, however, remains largely unknown. To answer this question, we first investigated the ability of ellagic acid to influence the activity of the estrogen receptor subtypes ERalpha and ERbeta in HeLa cells. Cells co-transfected with an estrogen response element (ERE)-driven luciferase (Luc) reporter gene and an ERalpha- or ERbeta-expression vector were exposed to graded concentrations of ellagic acid. At low concentrations (10(-7) to 10(-9) M), this compound displayed a small but significant estrogenic activity via ERalpha, whereas it was a complete estrogen antagonist via ERbeta. Further evaluation revealed that ellagic acid was a potent antiestrogen in MCF-7 breast cancer-derived cells, increasing, like the pure estrogen antagonist ICI182780, IGFBP-3 levels. Moreover, ellagic acid induced nodule mineralization in an osteoblastic cell line (KS483), an effect that was abolished by the estrogen antagonist. Endometrium-derived epithelial cells (Ishikawa) showed no response to the natural compound by using a cell viability assay (MTT). These findings suggest that ellagic acid may be a natural selective estrogen receptor modulator (SERM). 相似文献
4.
Malandrakis Anastasios A. Apostolidou Zoi A. Markoglou Anastasios Flouri Fotini 《European journal of plant pathology / European Foundation for Plant Pathology》2015,142(3):489-499
European Journal of Plant Pathology - 42 field isolates of Alternaria alternata collected from infected tomato fruit of processing plants located in the region of Pelloponisos, South Greece, were... 相似文献
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Influence of combined grazing by wild boar and small ruminant on soil and plant nutrient contents in a coppice oak forest 
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下载免费PDF全文 Zoi M. Parissi Athanassios Papaioannou Eleni M. Abraham Apostolos P. Kyriazopoulos Paraskevi Sklavou Constantinos N. Tsiouvaras 《植物养料与土壤学杂志》2014,177(5):783-791
Limited information is available about the impact of grazing on soil and plant nutrient contents in Mediterranean deciduous oak forests grazed by both livestock and wild life. Thus, the main objective of this study was to investigate the effect of wild boar and small ruminants grazing in a coppice oak forest in N Greece. Eight even‐aged stands of similar canopy cover and site quality were selected: four stands with long‐time grazing mainly by wild boar and four stands with long‐time grazing by sheep and goats. A plot of 150 m2 in each of the stands was fenced in order to be protected from grazing. A similarly sized plot open to grazing was assigned next to the fenced plot. Species composition was recorded in fenced and grazed plots. Soil samples and individual plants of the dominant palatable grass species Dactylis glomerata and Brachypodium sylvaticum were collected in every fenced and grazed plot. These samples were analysed for contents of nutrients. The results indicated that grazing increased organic matter, C, and total N, P and Ca, while it had no effect on the plant nutrients Mg, Na, K and all micronutrients, and pH as well. Wild boar grazing had a significantly negative impact on the concentrations of Na, Fe, Zn, and Mn in the soil. Beside a decrease in K and Mn, grazing had minimal effects on the contents of plant nutrients. Conversely, wild boar grazing increased the contents of several nutrients such as N, Na, Cu and Fe in plant tissue. It seems that species composition was more affected by the topography and management than by the soil features as revealed by RDA analysis. The results of this study indicate that it is important to maintain moderate small ruminant grazing intensity for the sustainable multi‐disciplinary management of coppice oak forests. It is also important to control wild boar population in order to facilitate ecosystem process, soil preservation and forage quality. 相似文献
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Anastasios A. Malandrakis Zoi A. Apostolidou Dimitra Louka Anastasios Markoglou Fotini Flouri 《European journal of plant pathology / European Foundation for Plant Pathology》2018,152(1):199-211
Field isolates of Alternaria alternata collected from tomato processors were characterized for sensitivity to respiration inhibitors using in vitro mycelial growth assays. Pyraclostrobin (QoI), boscalid, fluopyram and isopyrazam (SDHIs) mean EC50 values were 0.32, 1.43, 2.21, and 3.53 μg/ml respectively. Of the 42 isolates, 36 were sensitive to all respiration inhibiting fungicides tested whereas three isolates were less sensitive to boscalid, one to pyraclostrobin and two were simultaneously resistant to both inhibitors and isopyrazam. Correlation analysis between fungicide sensitivities revealed a positive cross-resistance between pyraclostrobin and tebuconazole, and between cyprodinil and mancozeb. There was no cross-resistance between QoIs, SHDIs or any other mode of action. Sequencing of the QoI and SDHI targets revealed the G143A cytochrome b resistance mutation in all pyraclostrobin-resistant isolates while analysis of the succinate dehydrogenase coding gene revealed point mutations in two of three of the gene subunits analyzed in boscalid-resistant isolates. Specifically, two isolates carried the H277Y and three the H133Q resistance mutations located in the sdhB and sdhD subunits of the respiration complex II, respectively. Isolates bearing the H277Y mutation also carried the G143A cytochrome b resistance mutation. Boscalid and pyraclostrobin-resistant isolates exhibited greater pathogenicity and sporulation compared to sensitive isolates, respectively. Isolates with cross-resistance exhibited greater pathogenicity and sporulation but slower mycelial growth compared to sensitive isolates. This is the first report of field isolates of A. alternata with single or double resistance to QoIs and SDHIs in Greece and should be considered in planning and implementing effective anti-resistance strategies. 相似文献
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A. P. Kyriazopoulos E. M. Abraham Z. M. Parissi Z. Koukoura A. S. Nastis 《Grass and Forage Science》2013,68(1):72-82
Production and nutritive value of forage in silvopastural systems can be improved by introducing shade‐ tolerant grass and legume species in appropriate mixtures. The management of these systems can present a challenge regarding the selection of the proper grass and legume species as well as the maintenance of the optimum balance between the two species in the grass–legume stand. The objectives of the present study were to evaluate the performance of pure stands and mixtures (75:25, 50:50, 25:75) of Dactylis glomerata:Trifolium subterraneum under full sun, 60% shade and 90% shade. Dry matter production of D. glomerata was not affected by shading, while that of T. subterraneum was drastically reduced. Shading increased the crude protein (CP) and acid detergent lignin content, but did not affect the acid detergent fibre and neutral detergent fibre content. The dry matter production of the 75:25 and 25:75 mixtures was higher compared to the one of the 50:50 mixture, while the CP content of the 75:25 mixture was the highest under moderate shading. However, relative yield, aggressivity index and competitive balance index indicated higher competitive ability for D. glomerata compared with T. subterraneum especially under shade. Therefore, the 25:75 mixture is suggested as the most suitable under moderate shade (60%), to perpetuate the stand and avoid the frequent re‐sowing of T. subterraneum. 相似文献
8.
Concern about poor natural regeneration of blue oak in California has prompted efforts to artificially regenerate this species. Two studies examined the best time to collect acorns and the effect various pre-storage treatments, including soaking and drying, have on germination. Results indicated that acorns can be successfully collected over a fairly wide interval, extending from late August until late October. Acorns from all harvest dates had high germination, as long as they were not allowed to dry out before storage. However, the earlier the acorns were collected, the earlier they tended to germinate. Soaking acorns for a day prior to storage had little effect. Drying acorns, however, reduced both the rate and amount of germination. A 10% reduction in moisture content resulted in almost 40% less total germination, and all acorns that lost 25% or more of their mositure failed to germinate during the 10-week test interval. To ensure good seed quality, blue oak acorns should be collected directly from tree branches and placed immediately in cold storage. 相似文献
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The competition effects of the perennial bunch-grass (Elymus glaucus B.B.) on the growth and survival of the oak seedlings (Quercus douglasii H. & A.) were investigated. There were four levels of Elymus competition, replicated three times. The three densities ofElymus employed were zero (control), 50 (Low — ‘L’ -), 116 (Medium — ‘M’ -) and 199 (High — ‘H’ -) plants m?2. Rates of soil water depletion, stomatal conductance, transpiration, shoot elongation and leaf expansion rates were measured between 23 March and 26 May 1988. Rates of soil water depletion, stomatal conductance and transpiration differed amongst the treatments and were higher in the control for the duration of the experiment. Shoot elongation rate (SER) and leaf expansion rate (LER) of blue oak seedling were directly related to soil water potentials. Zero values of LER rates for all treatments were observed at soil water potentials lower than?1.91 MPa, and concurrent reductions of stomatal conductance indicated stomatal closure due to the soil water deficit. In the control treatment, transpiration alone was not high enough to deplete soil moisture and to reduce LER of the oak seedlings. Leaf dessication occurred first in the H and M treatments (53% of seedlings dessicated) and two weeks later in the L treatment (37% dessicated) when the soil water potential was approximately ?4.0 MPa. The number of reproductive tillers and seed dry weight indicated thatElymus plants were under water stress from April 25 and concluded on May 25 with an early summer dormancy in all treatments. Data indicated that light intensity of 50% of ambient did not limit the development of oak seedlings. The results suggested that density of the perennial bunch-grassElymus glaucus lower than 50 plants m?2 could allow survival and successful establishment of blue oak in understories. 相似文献
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