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By using RACE (rapid amplification ofcDNA ends) based homologous cloning strategy, we have successfully isolated the genomic and full-length cDNA sequences of a gene encoding typical DFR (dihydroflavonol-4-reductase) from black-seeded Brassica campestris L. var. oleifera DC.. The gene, designated BcDFR here, is 1 722bp in length and harbors 5 introns with typical splice sites of plant DFR genes. BcDFR cDNA is 1311bp in length with a 1 158bp ORF as well as a 25bp 5‘ UTR and a 128bp 3‘ UTR. The encoded BcDFR protein is 385 aa with a calculated Mw of 42.85kD and a pI value of 5.55. The nucleotide and amino acid sequences of this gene share extensive homologies to plant DFR genes of wide origins especially high similarities to Cruciferous DFR genes. Sequence analyses such as phylogenetic analysis, conserved domain search and substrate specificity region detection all indicated that BcDFR gene is a quite potentially biofunctional gene. Its cloning enables us to further dissect the possible relatedness between DFR gene and Brassica seed coat color traits and to create transgenic novel yellow-seeded rapeseed germplasm through antisense- or RNAi-suppression of DFR gene expression in black-seeded elite cultivars. 相似文献
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生长调节剂和外植体对油菜离体培养愈伤诱导的影响 总被引：1，自引：0，他引：1
以双低优质油菜品种中双 7号的无菌苗为材料 ,探索了以子叶、下胚轴和幼根为外植体 ,及不同生长调节剂组合对愈伤诱导的影响作用。结果表明 ,生长调节剂是愈伤诱导成功的关键因子 ,2 ,4-D是诱导愈伤所必需的 ,以MS培养基含 1mg·L- 1 2 ,4-D与 2mg·L- 1 6 -BA配比为佳 ;同时子叶优于下胚轴和幼根作为外植体进行愈伤诱导 ,可获得出愈率高、增殖快、质量好的愈伤组织 相似文献
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冷季型草坪草抗旱机理研究进展 总被引：2，自引：0，他引：2
综述了冷季型草坪草抗旱机理的研究进展，重点介绍了冷季型草坪草的渗透调节能力和蒸腾速率等生理生化方面的研究进展，并简述了冷冬型草坪草的根、茎、叶对干旱协迫的反应研究和冷季型草坪草抗旱机理研究中存在的一些问题及研究趋势。 相似文献
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