Pigeonpea improvement: An amalgam of breeding and genomic research     

Sameer Kumar Chanda Venkata  Ganga Rao Nadigatla Veera Prabha Rama  Rachit K. Saxena  Kulbhushan Saxena  Hari D. Upadhyaya  Moses Siambi  Said N. Silim  Kothapally Narasimha Reddy  Anupama J. Hingane  Mamta Sharma  Shivali Sharma  Stephen Dominic Lyimo  Rose Ubwe  Meshack Makenge  Kananji Gad  Paul Kiprotich Kimurto  Manuel Amane  Kennedy Kanenga  Yuventino Obong  Emanuel Monyo  Chris Ojiewo  Nagesh Kumar Mallela Venkata  Jaganmohan Polineni Rao  Prashanthi Lakkireddy  Sudhakar Chourat  Indraprakash Singh  Sobhan Sajja  Shruthi Hirikara Beliappa  Rajeev K. Varshney 《Plant Breeding》2019,138(4):445-454

In the past five decades, constant research has been directed towards yield improvement in pigeonpea resulting in the deployment of several commercially acceptable cultivars in India. Though, the genesis of hybrid technology, the biggest breakthrough, enigma of stagnant productivity still remains unsolved. To sort this productivity disparity, genomic research along with conventional breeding was successfully initiated at ICRISAT. It endowed ample genomic resource providing insight in the pigeonpea genome combating production constraints in a precise and speedy manner. The availability of the draft genome sequence with a large‐scale marker resource, oriented the research towards trait mapping for flowering time, determinacy, fertility restoration, yield attributing traits and photo‐insensitivity. Defined core and mini‐core collection, still eased the pigeonpea breeding being accessible for existing genetic diversity and developing stress resistance. Modern genomic tools like next‐generation sequencing, genome‐wide selection helping in the appraisal of selection efficiency is leading towards next‐generation breeding, an awaited milestone in pigeonpea genetic enhancement. This paper emphasizes the ongoing genetic improvement in pigeonpea with an amalgam of conventional breeding as well as genomic research.  相似文献   

Broad-few-leaflets and outwardly curved wings: two new mutants of chickpea   总被引：3，自引：0，他引：3  

P. M. Gaur  V. K. Gour 《Plant Breeding》2003,122(2):192-194

This study was aimed at the induction of morphological mutations for increasing genetic variability and making available additional genetic markers for linkage studies in chickpea (Cicer arietinum L.). A wilt‐resistant, well‐adapted chickpea cultivar of central India,‘JG 315’(Jawahar gram 315), was used for the induction of mutations. Seeds presoaked in distilled water for 2 h were treated with ethyl methane sulphonate (EMS) using six different concentrations (0.1, 0.2, 0.3, 0.4, 0.5 and 0.6%) and two different durations (6 and 8 h). Several morphological mutants were identified in M₂. One of the mutants, isolated from a treatment of 0.3% EMS for 8 h, had five to nine large leaflets per leaf in comparison with 11‐17 normal‐sized leaflets per leaf observed in the parental cultivar ‘JG 315′. The mutant was designated broad‐few‐leaflets. Many leaves of this mutant showed a cluster of three to five overlapping leaflets at the terminal end. The other mutant, designated outwardly curved wings, was isolated from the 0.5% EMS treatment for 6 h. In this mutant, the wings were curved outwards, exposing the keel petal, while the wings in typical chickpea flowers are incurved and enclose the keel. The lines developed from the broad‐few‐leaflets and outwardly curved wings mutants were named JGM 4 (Jawahar gram mutant 4) and JGM 5, respectively. Inheritance studies indicated that each of these mutant traits is governed by a single recessive gene. The gene for broad‐few‐leaflets was designated bfl and the gene for outwardly curved wings was designated ocw. The locus bfl was found to be linked with the locus lg (light green foliage) with a map distance of 18.7 ± 6.3 cM.  相似文献   

A gene producing one to nine flowers per flowering node in chickpea     

P.M. Gaur  V.K. Gour 《Euphytica》2002,128(2):231-235

Chickpea (Cicer arietinum L.) has a racemose type of inflorescence and at each axis of the raceme usually one or two and rarely three flowers are borne. Plants producing 3 to 9 flowers, arranged in acymose inflorescence, at many axis of the raceme, were identified in F₂ of an interspecific cross ICC 5783 (C. arietinum) × ICCW 9 (C. reticulatum)in which both the parents involved were single-flowered. A spontaneous mutation in one of the two parents or in the F₁was suspected. However, the possibility for establishment of a rare recombination of two interacting recessive genes could not be ruled out. The number of pods set varied from 0 to 5 in each cyme. Inheritance studies indicated that a single recessive gene, designated cym, is responsible for cymose inflorescence. The allelic relationship of cym with sfl, a gene for double-flowered trait, was studied from a cross involving multi flowered plants and the double-flowered line ICC 4929. Thecym gene was not allelic to sfl, suggesting that two loci control the number of flowers per peduncle in chickpea. The cym locus segregated independently of the locus sfl, ifc (inhibitor of flower color) and blv (bronze leave). This revised version was published online in August 2006 with corrections to the Cover Date.  相似文献   

Identification of a non-redundant set of 202 in silico SSR markers and applicability of a select set in chickpea (Cicer arietinum L.)     

Gaurav Agarwal  Murali M. Sabbavarapu  Vikas K. Singh  Mahendar Thudi  S. Sheelamary  Pooran M. Gaur  Rajeev K. Varshney 《Euphytica》2015,205(2):381-394

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Estimation of genetic components of variation for salt tolerance in chickpea using the generation mean analysis     

Srinivasan Samineni  Pooran M. Gaur  Timothy D. Colmer  L. Krishnamurthy  Vincent Vadez  Kadambot H. M. Siddique 《Euphytica》2011,182(1):73-86

Chickpea (Cicer arietinum L.) is known to be salt-sensitive and in many regions of the world its yields are restricted by salinity. Recent identification of large variation in chickpea yield under salinity, if genetically controlled, offers an opportunity to develop cultivars with improved salt tolerance. Two chickpea land races, ICC 6263 (salt sensitive) and ICC 1431 (salt tolerant), were inter-crossed to study gene action involved in different agronomic traits under saline and control conditions. The generation mean analysis in six populations, viz. P₁, P₂, F₁, F₂, BC₁P₁ and BC₁P₂, revealed significant gene interactions for days to flowering, days to maturity, and stem Na and K concentrations in control and saline treatments, as well as for 100-seed weight under salinity. Seed yield, pods per plant, seeds per plant, and stem Cl concentration were controlled by additive effects under saline conditions. Broad-sense heritability values (>0.5) for most traits were generally higher in saline than in control conditions, whereas the narrow-sense heritability values for yield traits, and stem Na and K concentrations, were lower in saline than control conditions. The influence of the sensitive parent was higher on the expression of different traits; the additive and dominant genes acted in opposite directions which led to lower heritability estimates in early generations. These results indicate that selection for yield under salinity would be more effective in later filial generations after gene fixation.  相似文献   

Comparative genomics of trypanosomatid parasitic protozoa     

El-Sayed NM  Myler PJ  Blandin G  Berriman M  Crabtree J  Aggarwal G  Caler E  Renauld H  Worthey EA  Hertz-Fowler C  Ghedin E  Peacock C  Bartholomeu DC  Haas BJ  Tran AN  Wortman JR  Alsmark UC  Angiuoli S  Anupama A  Badger J  Bringaud F  Cadag E  Carlton JM  Cerqueira GC  Creasy T  Delcher AL  Djikeng A  Embley TM  Hauser C  Ivens AC  Kummerfeld SK  Pereira-Leal JB  Nilsson D  Peterson J  Salzberg SL  Shallom J  Silva JC  Sundaram J  Westenberger S  White O  Melville SE  Donelson JE  Andersson B  Stuart KD  Hall N 《Science (New York, N.Y.)》2005,309(5733):404-409

A comparison of gene content and genome architecture of Trypanosoma brucei, Trypanosoma cruzi, and Leishmania major, three related pathogens with different life cycles and disease pathology, revealed a conserved core proteome of about 6200 genes in large syntenic polycistronic gene clusters. Many species-specific genes, especially large surface antigen families, occur at nonsyntenic chromosome-internal and subtelomeric regions. Retroelements, structural RNAs, and gene family expansion are often associated with syntenic discontinuities that-along with gene divergence, acquisition and loss, and rearrangement within the syntenic regions-have shaped the genomes of each parasite. Contrary to recent reports, our analyses reveal no evidence that these species are descended from an ancestor that contained a photosynthetic endosymbiont.  相似文献   

Serodiagnosis of porcine cysticercosis by enzyme-linked immunosorbent assay (ELISA) using fractionated antigens   总被引：1，自引：0，他引：1  

D Kumar  S N Gaur 《Veterinary parasitology》1987,24(3-4):195-202

The sensitivity and specificity of the enzyme-linked immunosorbent assay (ELISA) for the diagnosis of Taenia solium cysticercosis was evaluated in experimentally and naturally infected pigs, using T. solium larval scoleces and its fractionated 1st and 2nd peaks on Sephadex G-200 as antigens. First peak antigen gave maximum sensitivity and highest antibody titres. The overall sensitivity of this test was found to be 91.5, 95.8 and 70.8% with scolex, 1st and 2nd peak antigens, respectively. False positive reactions occurred in 9.09% of uninfected pigs with scolex and 1st peak antigens and cross-reactions occurred in 25% of Taenia hydatigena-infected animals using scolex and 2nd peak antigens. No cross-reaction was observed using 1st peak antigen. The specificity of the test was 92.3, 96.2 and 92.3% with scolex, 1st and 2nd peak antigens, respectively.  相似文献   

Forest utilization patterns and socio-economic status of the Van Gujjar tribe in sub-Himalayan tracts of Uttarakhand, India     

Jyotsana SHARMA  Sumeet GAIROLA  R.D.GAUR  R.M.PAINULI 《中国林学(英文版)》2012,14(1):36-46

The present study was conducted on the Van Gujjar tribe inhabiting a sub-Himalayan tract in the North Western Himalayas of Uttarakhand State,India.The Van Gujjars have been practicing transmigration over hundreds of years.They migrate each year with their households and livestock between summer and winter pastures.A few years ago with the announcement of the establishment of the Rajaji National Park,the tribe has been forced out of the forest area and rehabilitated outside the park,which has affected their lifestyle.The newly established rehabilitation colony in the Gandikhata area of Haridwar District of Uttarakhand State was taken as a case study.The aims of the present study were to understand and evaluate the socio-economic status of the Van Gujjars in their newly established rehabilitation colony,the utilization pattern of forest resources by the tribe and their relative preference for selective trees for various uses.A total of 176 households were interviewed (giving equal weight to all economic classes and family every size) by using pre-structured questionnaires.The education level was very low (12.9%) and the average income per household was recorded as Rs.36000 (approximately $ 803) per year.The major source of income was dairy production (80.6%) followed by labor employment (13.9%),NTFPs (4.2%) and agricultural production (1.4%).More than 90% of fuel wood and fodder is extracted from the forest.The average fuel wood and fodder consumptions per household per day were recorded as 25.86 and 21.58 kg,respectively.A total of 35 species of cultivated plants and 89 species of wild plants were found to be utilized as food sources.Selectively 25 wild tree species are well known as being used by the Van Gujjars as fodder,fuel wood,agricultural implements,household articles,dye,medicine,fiber and other products.According to their utility value,the most preferred and useful tree species is Ougeinia oojeinensis,followed by Terminalia alata,Bombax ceiba,Shorea robusta and Dalbergia sissoo.  相似文献   

Assessment of Genetic Diversity in Feronia limonia (L.) Swingle Using Inter Simple Sequence Repeats     

Shailendra Vyas  Anupama Gaur  Akhilesh K. Tyagi 《Journal of Sustainable Forestry》2013,32(3):328-342

ABSTRACT

Genetic variation was studied in threatened natural populations of Feronia limonia (Rutaceae). Samples were collected from 3 populations located in the Aravalli mountain range of south-east Rajasthan, India. Using 25 Inter Simple Sequence Repeat (ISSR) primer combinations, significant diversity was characterized among the individuals. Analysis of Molecular Variance (AMOVA) revealed that diversity is partitioned mainly within population components (80%). F statistics showed that low but significant amount of gene flow is taking place between populations (φ_ST = 0.21, P < .001). The study highlights the utility of ISSRs in assessing diversity of populations that are fragmented or limited in size.  相似文献   

Assessment of meat quality defect genes in indigenous pigs of Bareilly region     

Saini  B. L.  Gaur  G. K.  Sahoo  N. R.  Naha  B. C.  Baranwal  A. 《Tropical animal health and production》2019,51(6):1329-1335

This investigation was undertaken to assess the population of indigenous (Bareilly local) pigs for meat quality genes (RYR1, PRKAG3, HFABP, MYF-5, and MC4R). The results showed that indigenous pigs were monomorphic at RYR1locus (100% NN genotype), HFABP locus (100% HH genotype), and MYF-5 locus (100% DD genotype). Homozygote RR and heterozygote QR genotypes were observed at PRKAG3 (c.599 G>A) SNP locus with 89 and 11% frequency. The frequency of wild (R) and mutant (Q) allele at the said locus was 95 and 5%. The MC4R SNP had three genotypes; homozygote AA with 5% frequency, heterozygote AG with 53% frequency, and homozygote GG with 42% frequency. Corresponding frequency of A and G allele was 32 and 68%, respectively. Monomorphic status at RYR1locus for NN genotype, HFABP locus for HH genotype, and MYF-5 locus for DD genotype indicated that favorable genes for quality pork production have been fixed in the population. The higher frequency of RR genotype (89%) at PRKAG3 and GG genotype (42%) at MC4R locus further explained the existence of favorable genotypes in indigenous pigs.

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