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Maria?Teresa?Dell'AbateEmail author Anna?Benedetti Alessandra?Trinchera Daniele?Galluzzo 《Biology and Fertility of Soils》2003,37(2):124-129
Carbon and nitrogen mineralisation of leather meal fertilisers were studied in two soils characterised by different respiration activity. Both C and N mineralisation were highest in the most active soil, and when leather meal was added as a powder rather than as 2- to 4- and 4- to 6-mm particles. Fast and slow soluble N pools were determined after extraction with cold water and with hot buffer, respectively. The N remaining after the second extraction with hot buffer was named slow-release N. The percentage of slow-release N rose as the size of the applied leather meal particles increased, whereas fast soluble N was highest in the coarsest (4-6 mm) fertiliser. 相似文献
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J. M. Lynch A. Benedetti H. Insam M. P. Nuti K. Smalla V. Torsvik P. Nannipieri 《Biology and Fertility of Soils》2004,40(6):363-385
This review mainly discusses three related topics: the application of ecological theories to soil, the measurement of microbial diversity by molecular techniques and the impact of transgenic plants and microorganisms on genetic diversity of soil. These topics were debated at the Meeting on Soil Emergency held in Erice (Trapani, Italy) in 2001 for the celebration of the 50th anniversary of the Italian Society of Soil Science. Ecological theories have been developed by studying aboveground ecosystems but have neglected the belowground systems, despite the importance of the latter to the global nutrient cycling and to the presence of life on the Earth. Microbial diversity within the soil is crucial to many functions but it has been difficult in the past to determine the major components. Traditional methods of analysis are useful but with the use of molecular methods it is now possible to detect both culturable and unculturable microbial species. Despite these advances, the link between microbial diversity and soil functions is still a major challenge. Generally studies on genetically modified bacteria have not addressed directly the issue of microbial diversity, being mainly focused on their persistence in the environment, colonization ability in the rhizosphere, and survival. Concerns have been raised that transgenic plants might affect microbial communities in addition to environmental factors related to agricultural practice, season, field site and year. Transgenic plant DNA originating from senescent or degraded plant material or pollen has been shown to persist in soil. Horizontal transfer of transgenic plant DNA to bacteria has been shown by the restoration of deleted antibiotic resistance genes under laboratory in filter transformations, in sterile soil or in planta. However, the transformation frequencies under field conditions are supposed to be very low. It is important to underline that the public debate about antibiotic resistant genes in transgenic plants should not divert the attention from the real causes of bacterial resistance to antibiotics, such as the continued abuse and overuse of antibiotics prescribed by physicians and in animal husbandry. 相似文献
3.
Multi‐element stable isotopic dilution and multi‐surface modelling to assess the speciation and reactivity of cadmium and copper in soil 
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下载免费PDF全文 Z. L. Ren Y. Sivry J. Dai M. Tharaud L. Cordier M. F. Benedetti 《European Journal of Soil Science》2015,66(6):973-982
Chemical extraction, multi‐element stable isotopic dilution (ID) and multi‐surface modelling were used to investigate the lability of cadmium (Cd) and copper (Cu) in nine types of soil with different properties and contaminated or not with Cd and Cu. The chemical extraction and ID analyses both showed that Cd was more labile than Cu in all the soil types studied. From the ID results, 32.8–93.3% of total Cd and 14.7–71.8% of total Cu were isotopically exchangeable after 3 days of equilibration. A single extraction in 0.43 m HNO3 gave similar results to the 3‐day ID assay for Cu in most of the soils and for Cd in the non‐calcareous soils. However, an eight‐step selective sequential extraction (SSE) procedure gave different results from the ID assay for both metals. Predictions of the multi‐surface model for the amounts of Cd and Cu adsorbed, based on measured metal ion activities in the soil solution and the concentrations of reactive surfaces in the soil, agreed with the ID results. The model predicted that soil organic matter was the predominant sorbent for Cd and Cu in the soils and that manganese oxide was the least important sorbent. The contributions of iron oxides to sorption were predicted to be small except in soil with a high pH and little organic matter. The predicted sorption on different soil components did not match SSE measurements. 相似文献
4.
Radaelli E Castiglioni V Losa M Benedetti V Piccinini R Nicholas RA Scanziani E Luini M 《Research in veterinary science》2011,91(2):251-253
This report describes an outbreak of Mycoplasma bovis mastitis affecting 45 cows in a herd of 122 dairy cattle in Northern Italy. Clinically, the outbreak was characterized by agalactia, multiple swollen and painless quarters, high milk somatic cell count and unresponsiveness to conventional antibiotic therapy. M. bovis was isolated from the milk samples of all the 32 affected cows tested and from the mammary tissue of three affected cows that underwent necropsy. No other pathogens were isolated from these samples. Lesions in two of the necropsied cows were characterized by mild chronic suppurative mastitis and galactophoritis. The other necropsied cow showed a chronic necrosuppurative and pyogranulamaous galactophoritis, a condition not previously associated with M. bovis. M. bovis was detected immunohistochemically in the lumen of the affected mammary ducts suggesting that ascending infection via the teat canal was the likely route of transmission. No other intralesional pathogens were demonstrated microscopically. 相似文献
5.
Alessandra Lagomarsino Anna Benedetti Sara Marinari Letizia Pompili M. Cristina Moscatelli Pier Paolo Roggero Roberto Lai Luigi Ledda Stefano Grego 《Biology and Fertility of Soils》2011,47(3):283-291
Five soils characterised by different agro-forest managements, typical of Mediterranean environment and with increasing human
impact were chosen in Sardinia (Italy): two vineyards with different management systems, a rotation hay crop-pasture and a
forest (Quercus suber L.). The study aimed to investigate the relationships between C storage and microbial functionality in soil under different
managements. Pools of total organic C and microbial biomass C were determined, as well as the loss of organic C due to microbial
respiration (basal and cumulative) and several microbial indices (metabolic, mineralization, and microbial quotient) as indicators
of the microbial efficiency in the use of energy and the degree of substrate limitation for soil microbes. Enzymes were chosen
on their relevance in the C (β-cellobiohydrolase, N-acetyl-β-glucosaminidase, β-glucosidase, α-glucosidase), N (leucine aminopeptidase), S (arylsulphatase) and P (acid phosphatase)
cycling and were used as indicators of functional diversity in soil. Organic C pools and enzyme activities on average increased
noticeably in soils with a lower human impact showing the highest values in forest and the lowest in the vineyards, following
the trend of organic matter availability. The trend in functional diversity reflected the increase of microbial pool and organic
C availability: the vineyards showed a lower Shannon’s diversity index, whilst pasture and forest sites reached the maximum
levels of functional diversity. These soils showed an increase of microbial efficiency in the use of available resources and
the decrease of substrate limitation for soil microbes. 相似文献
6.
Microbial dynamics after adding bovine manure effluent together with a nitrification inhibitor (3,4 DMPP) in a microcosm experiment 总被引：1，自引：0，他引：1
Anita Maienza Erland Bååth Silvia Rita Stazi Anna Benedetti Stefano Grego Maria Teresa Dell’Abate 《Biology and Fertility of Soils》2014,50(6):869-877
The application of animal manure effluents in agriculture in combination with nitrification inhibitors should be beneficial for nutrient recycling, soil quality, plant productivity, and greenhouse gas emission and offer economic advantages to make them an alternative to conventional fertilizers. The present study aims to estimate the effects of the addition of bovine manure effluent alone or together with a nitrification inhibitor (3,4-dymethylpyrazol-phosphate (3,4 DMPP)) on the microbial community dynamics in a Mediterranean soil in an incubation experiment over 28 days. The application of the bovine manure effluent increased respiration, microbial biomass carbon, fungal and bacterial growth, and enzyme activities and changed the microbial community structure evaluated by the phospholipid fatty acid pattern. Adding the bovine manure effluent together with the nitrification inhibitor, although partly negating the positive effect of the effluent on soil microbial activity, still resulted in higher or similar growth and activity as in the control. Our results indicate that the addition of the nitrification inhibitor 3,4 DMPP together with a bovine manure effluent could be a promising solution to control the animal manure effluent application effects on soil microbiological properties and microbial dynamics, as well as counteracting direct inhibiting effects of 3,4 DMPP on the soil heterotrophic community. 相似文献
7.
L. De Benedetti G. Burchi A. Mercuri N. Pecchioni P. Faccioli T. Schiva 《Plant Breeding》2000,119(5):443-445
Random amplified polymorphic DNA (RAPD) markers were used to verify interspecific hybridization in Alstroemeria. Five putative interspecific hybrids and their parents were analysed by means of four preselected RAPD primers. The putative parentage was confirmed in four hybrids and was excluded in one that showed completely different RAPD patterns from its putative parents and a different phenotype. Our results demonstrated that this molecular technique is a powerful tool for verifying hybridity rapidly if the putative parents are given. This tool will allow screening of small immature seedlings for verification of hybridity and should improve the efficiency of breeding programmes. 相似文献
8.
Leonardo Recena Aydos Luane Aparecida do Amaral Ana Cristina Jacobowski Roberta Serafim de Souza Eduardo Benedetti Parisotto Mariana Biava de Menezes Felipe Francisco Bittencourt Junior Emely Schuindt Fernandes Iandara Schettert Silva Luciane Candeloro Portugal Cláudio Gonçalves Oliveira Gustavo Tsuyoshi Senra Masuko Leandro Fontoura Cavalheiro Carlos Eduardo Domingues Nazário Elisvânia Freitas dos Santos Maria Lígia Rodrigues Macedo 《Journal of animal physiology and animal nutrition》2021,105(2):364-375
Metabolic syndrome (MetS) and obesity are growing in many parts of the world, becoming public health problems. It is proposed that foods with functional properties can assist in the treatment of these diseases. Crude buriti pulp oil (BPO) is a food traditionally consumed by residents in the Pantanal, Cerrado and Brazilian Amazon. It is rich in oleic acid, tocopherols and carotenoids, emerging as a potential functional food. Thus, this study aimed to evaluate the effect of the supplementation of BPO on metabolic disorders caused by a high-fat diet. Four groups of C57BL6 mice were used, a lean group with AIN-93M diet and control oil supplementation, an obese group with a high-fat diet and control oil supplementation, and two obese groups with a high-fat diet and BPO supplementation in the amounts of 50 and 100 mg/kg. BPO worsened the metabolic state caused by the high-fat diet, worsening risk factors associated with MetS, as the abdominal circumference and retroperitoneal fat, serum levels of total cholesterol, uric acid, alanine transaminase, glucose and triglycerides, and renal fat, in addition to changes in glycaemic control and oxidative stress markers. C57BL/6 mice fed with a high-fat diet and supplemented with BPO presented a worsening in metabolic risk factors associated with MetS. 相似文献
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10.
Cardoso SR Queiroz LB Goulart VA Mourão GB Benedetti E Goulart LR 《Research in veterinary science》2011,91(3):e107-e112
Candidate genes have been associated with milk production in bovines, such as the diacylglycerol O-acyltransferase 1 (DGAT1) and leptin (LEP); however, they have not been simultaneously investigated nor have been evaluated in the Brazilian Girolando breed (Gir × Holstein, backcrossed to Holstein). Our aim was to determine the influence of fat-related genes, DGAT1 and LEP, and their polymorphisms on performance traits of milk production in the Girolando breed. Results indicated that the K allele of the DGAT1 gene showed a significant association with total and average daily milk production with additive effect. The LEP gene showed that the A allele and its homozygote are highly prevalent and almost fixed in this population and may have been favorably selected during backcrossing for the origin of this breed. The important impact of the K allele of the DGAT1 gene on milk production corroborates the initiative of performing marker-assisted selections with this gene in breeding programs of the Girolando breed. 相似文献