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Pakull Birte Eusemann Pascal Wojacki Janine Ahnert Diana Liesebach Heike 《European Journal of Forest Research》2021,140(6):1543-1557
European Journal of Forest Research - In Germany, Douglas fir (Pseudotsuga menziesii Franco) is seen as a valuable species for future cultivation in times of climate change. Local seed production... 相似文献
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Development and application of functional markers in maize 总被引：8，自引：0，他引：8
Thomas Lübberstedt Imad Zein Jeppe Reitan Andersen Gerhard Wenzel Birte Krützfeldt Joachim Eder Milena Ouzunova Shi Chun 《Euphytica》2005,146(1-2):101-108
Summary Functional markers (FMs) are derived from polymorphic sites within genes causally involved in phenotypic trait variation (Andersen,
J.R. & T. Lübberstedt, 2003. Trends Plant Sci 8: 554–560). FM development requires allele sequences of functionally characterized
genes from which polymorphic, functional motifs affecting plant phenotype can be identified. In maize and other species with
low levels of linkage disequilibrium, association studies have the potential to identify sequence motifs, such as a few nucleotides
or insertions/deletions, affecting trait expression. In one of the pioneering studies, nine sequence motifs in the dwarf8 gene of maize were shown to be associated with variation for flowering time (Thornsberry, J.M., M.M. Goodman, J. Doebley,
S. Kresovich, D. Nielsen & E.S. Buckler, 2001. Nat Genet 28: 286–289). Proof of sequence motif function can be obtained by
comparing isogenic genotypes differing in single sequence motifs. At current, the most appropriate approach for this purpose
in crops is targeting induced local lesions in genomes (TILLING) (McCallum, C.M., L. Comai, E.A. Greene & S. Henikoff, 2000.
Nat Biotechnol 18: 455–457). In central Europe, maize is mainly grown as forage crop, with forage quality as major trait,
which can be determined as proportion of digestible neutral detergent fiber (DNDF). Brown midrib gene knock out mutations have been shown to be beneficial for forage quality but disadvantageous for overall agronomic performance.
Two brown midrib genes (bm1 and bm3) have been shown to be involved in monolignol biosynthesis. These two and additional lignin biosynthesis genes have been
isolated based on sequence homology. Additional candidate genes putatively affecting forage quality have been identified by
expression profiling using, e.g., isogenic bm lines. Furthermore, we identified an association between a polymorphism at the COMT locus and DNDF in a collection of European
elite inbred lines. 相似文献
3.
Quantitative trait loci mapping of forage agronomic traits in six mapping populations derived from European elite maize germplasm 
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下载免费PDF全文 Pengfei Leng Milena Ouzunova Matthias Landbeck Gerhard Wenzel Joachim Eder Birte Darnhofer Thomas Lübberstedt 《Plant Breeding》2018,137(3):370-378
Four agronomic traits were analysed including dry matter concentration (DMC) and dry matter yield (DMY) for stover, plant height (PHT) and days from planting to silking (DPS). We mapped quantitative trait loci (QTL) in three populations with doubled haploid lines (DHL), one RIL population and two testcross (TC) populations derived from crosses between two of the four populations mentioned above to elite tester lines, based on field phenotyping at multiple locations and years for each; 146–168 SSRs were used for genotyping of the four mapping populations. Significant high phenotypic and genotypic correlations were found for all traits at two locations, while DMC was negatively correlated with the other traits. A total of 42, 41, 54, and 45 QTL were identified for DMC, DMY, PHT, and DPS, respectively, with 9, 7, 12, and 7 major QTL for each trait. Most detected QTL displayed significant interactions with environment. Major QTL detected in more than two populations will contribute to marker‐assisted breeding and also to fine mapping candidate genes associated with maize agronomic traits. 相似文献
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Lise C. Deleuran Birte Boelt 《Acta Agriculturae Scandinavica, Section B - Plant Soil Science》2013,63(1):57-62
Abstract Establishment methods have proven to be of major importance for grass-seed production. The objective of this research was to test the effect of different sowing techniques on plant establishment and the subsequent seed yield. Perennial ryegrass (Lolium perenne L.) is used as the model grass due to its large importance in Danish agriculture. In a three-year trial six different methods of under-sowing of perennial ryegrass in a spring barley cover crop were employed. Perennial ryegrass was either sown directly at different depths within the spring barley (Hordeum vulgare L.) rows or placed 2, 6, or 12 cm from the spring barley rows. Results of dry-matter yield indicate that the best establishment of the grass occurred when placing the grass 6 or 12 cm from the cover-crop row, and this is of importance in less vigorous grasses. Overall, no seed-yield difference has been observed for perennial ryegrass when placing the grass 2, 6, or 12 cm from the cover-crop row. Placement of the ryegrass seed crop 6 cm from the cereal row showed that a significant yield increase of 34–71 kg ha?1 can be obtained compared with sowing in the cereal row. 相似文献
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Most agree that social and ecological approaches should be integrated to ensure sustainable management of natural resources. However, an analysis of the content of three problem-based journals shows that if social sciences are included at all, they are typically subservient to natural sciences, and that quantitative approaches are privileged. We argue that true integration is achievable only if natural sciences and social sciences are each robust and if they meet eye to eye. We call for more openness to stand-alone social science research in problem-based journals, especially to research using qualitative methods. We highlight the potential insights derived from studying decision makers at the microlevel: the pastoralists, farmers, ranchers, and foresters who make final management choices. We argue that publishing such qualitative social sciences promotes dialogue across disciplines, strengthens integration, and increases the real-world impact of research. 相似文献
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Lise C. Deleuran René Gislum Birte Boelt 《Acta Agriculturae Scandinavica, Section B - Plant Soil Science》2013,63(1):68-75
Seed production of spinach (Spinacea oleracea L.) is positively affected by increasing the amounts of nitrogen (N); however, regulations restrict the application rate of N in Danish agriculture. The aim of our study was to test different N application strategies in spinach seed production and their effects on N concentration, seed yield, seed weight and germination. A total amount of 100–150 kg N ha?1 was broadcast or placed at sowing or applied as a split application at sowing and pre-elongation at the Danish Institute of Agricultural Sciences, location Flakkebjerg. Seed yields were positively affected by the high N applications, but no difference was observed between 150 kg N ha?1 broadcast at sowing, and 40 kg N ha?1 broadcast at sowing with an additional late application at a rate based on soil mineral N analysis. No consistent pattern was observed between N management strategy and seed weight or N management strategy and germination. A high N concentration in the plants at the start of growth showed negative effects on final seed yield, whereas later in the season there was a significantly positive correlation between N concentration in the plants and seed yield. A soil mineral analysis can be used as a tool to adjust the N application rate when a split application strategy is chosen. 相似文献
9.
Venner M Reinhold B Beyerbach M Feige K 《Veterinary journal (London, England : 1997)》2009,179(2):301-303
The prophylactic application of azithromycin to prevent pulmonary abscesses in foals was evaluated on a stud with endemic Rhodococcus equi pneumonia. Forty-five foals served as untreated controls in two groups. Twenty-five foals were given azithromycin (10mg/kg) orally once daily for 4 weeks. The foals were examined once a week from birth to the age of 5 months. If clinical signs or leucocytosis were noted and pulmonary sonographic findings (diameter >10 mm) were observed, the diagnosis of abscessing pneumonia was made. The prevalence of pulmonary abscesses was similar in the control groups (31/45 foals), and in the azithromycin group (15/25 foals), but the foals in the azithromycin group were affected significantly later (median: day 83, range 67-123 days) (control groups: day 54, range 52-82; and 46, range 28-86 days). It was concluded that the application of azithromycin for 28 days post-natally does not reduce the prevalence of pulmonary abscesses in foals on a stud with endemic pneumonia. 相似文献
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Koethe M Pott S Ludewig M Bangoura B Zöller B Daugschies A Tenter AM Spekker K Bittame A Mercier C Fehlhaber K Straubinger RK 《Veterinary parasitology》2011,180(3-4):179-190
The protozoan parasite Toxoplasma (T.) gondii is one of the most common zoonotic infectious agents worldwide. Besides its sexual reproduction in cats, T. gondii can also infect a wide spectrum of other warm-blooded animals. These include animals used for human consumption such as pigs or chickens. Nevertheless, the role of turkeys for the epidemiology of T. gondii infections has not been studied thoroughly. We have established a kinetic ELISA (KELA) for the detection of T. gondii-specific IgG antibodies in turkey serum samples. The test is based on the recombinant dense granule antigens GRA7 and GRA8. These proteins were used as an antigen mixture at a concentration of 0.13 μg per well. The overall sensitivity of the assay was between 92.6% and 100% and the specificity ranged from 78.1% to 100%, depending on the method used to calculate these parameters. Using this KELA we examined 1913 turkey serum samples from 14 turkey farms from different areas of Germany. From these sera, 387 produced a signal in the KELA, corresponding to a true seroprevalence of up to 20.2%. The seropositivity rate in individual fattening cycles at individual farms ranged from 0.0% to 77.1%, whereas the rates were highly variable within the individual farms and individual fattening cycles. Consequently, conditions of animal husbandry could not be associated with particular seroprevalence rates. Although seropositivity cannot be linked directly to infectious tissue cysts in the muscle tissue of commercially produced turkey meat, we state that there is a potential risk of being infected by consuming turkey meat products that were not heat treated. 相似文献