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1.
Arisaema sikokianum (Araceae) native to Japan is classified as a vulnerable species in the Red Data Book of Japan. Control of dormancy is essential for efficient corm production and forcing culture. Sprouting of both vegetative and reproductive corms was enhanced by exposure to low temperature. Vegetative corms exposed to low temperatures at 5 °C longer sprouted faster when grown at 20 °C. Effective temperatures for breaking dormancy was 5 °C. Reproductive corms treated at 5 °C longer showed shorter days to flower. Successful forcing culture was achieved; corms treated at 5 °C from November for 30 days flowered on 5th February. 相似文献
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Nakamura K Murata C Ito M Iwamori T Nishimura S Hisamatsu S Sonoki S Nakayama A Suyama E Kawasaki H Taira K Nishino K Tachi C 《The Journal of reproduction and development》2006,52(1):73-80
As the first step in investigating the possiblity of applying ribozyme technology to artificial control of the sex ratios at birth in farm animals, where the demand for females exceeds that for males, we designed a hammerhead ribozyme (HHRz) and 2 tRNA(val)-hammerhead ribozyme complexes (tRNARz3 and tRNARz4), and examined their effects upon murine Sry mRNA in vitro and in cells. We demonstrated that HHRz and tRNARz3 could effectively cleave the target Sry mRNA in vitro. For the purpose of experiments in vivo, HHRz was cloned into the highly efficient pUC-CAGGS mammalian expression vector (pCAG/HHRz), and the tRNA ribozyme complexes were cloned into the pol III promoter-driven pPUR-KE vector (pPUR/tRNARz3 and pPUR/tRNARz4); the ribozyme vectors were co-transfected with the target vector (pCAG/Sry). A suppressive action (up to approx. 60%) was confirmed for pCAG/HHRz and pPUR/tRNARz3 upon the transiently expressed exogenously introduced Sry in M15 cultured cells. 相似文献
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为茶园生态控草提供理论依据,以茶单作茶园为对照,采用5点取样法研究茶-芋间作茶园夏季杂草的种类/群落结构及其防治效果。结果表明:2类茶园中主要杂草有20科37种,其中,茶-芋间作茶园有17科24种,杂草科的数目较对照(16科30种)多,但杂草种类数目较对照少。茶-芋间作同时期杂草田间密度和物种丰富度较对照显著降低,最高株防效达92%,夏季6-8月平均株防效为73. 6%;茶-芋间作可改变茶园杂草群落结构,显著降低优势杂草的危害程度。单作茶园的优势杂草为尼泊尔蓼、丛枝蓼、薄叶新耳草、野茼蒿和碎米荠,茶-芋间作茶园优势杂草为野茼蒿、薄叶新耳草、蕺菜、宽叶母草和尼泊尔蓼。 相似文献
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Morioka K Fukai K Ohashi S Sakamoto K Tsuda T Yoshida K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(7):653-658
At least two biotypes were observed at the 2nd passage stage after the isolation of Foot-and-mouth disease Virus (FMDV) O/JPN/2000 strain. These 2 types of viruses differed from their plaque phenotypes and were distinguishable by using a monoclonal antibody (MAb) 64G8 that was made for the FMDV O/JPN/2000 strain. One of these 2 biotypes formed small plaque (SP) and with immuno staining showed a positive reaction to MAb 64G8, while the other formed clear large plaque (LP) and did not react with MAb 64G8. The amino acid sequences of the capsid coding region (VP1-VP4) of the SP virus (SPV) and the LP virus (LPV) revealed two substitutions on the 133rd amino acid in VP2, and the 56th amino acid in VP3. These amino acid changes of SPV and LPV are Asn to Asp, Arg to His, respectively. The Arg of the 56th amino acid in VP3 that have been known as critical position of cell culture adapted virus. Only LPV showed high pathogenicity in suckling mice, and its LD(50) was calculated to be about 10(2) TCID(50)/0.1 ml. These results showed that the SPV that existed at the 2nd passage stage from isolation was a low virulence virus, which may suggest why the pathogenicity of O/JPN/2000 did not show clear symptoms in infected cattle. 相似文献
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Md. Masud Karim Nazmoon Naher Tonu Mohammad Shakhawat Hossain Taketo Funaki M. Bahadur Meah Delwar M. Hossain M. Asad ud-doullah Eigo Fukai Keiichi Okazaki 《Euphytica》2016,208(3):535-544
Low erucic acid (LEA) rapeseed, which has accumulated mutant fatty acid elongase genes at the BnFAE1.1 and BnFAE1.2 loci of the A- and C-genome, respectively, is an important oilseed crop. Short growing turnip rape (B. rapa) is also important as a catch crop in the continuous cropping of rice in Asia but there is no LEA B. rapa cultivar for cultivation in South Asia. In order to develop LEA turnip rape cultivars, high erucic acid turnip rape cultivars were interspecifically crossed as recurrent parents to a canola quality rapeseed. In the meantime, we monitored incorporation of the mutant bnfae1.1 (e1) gene into A-genome of turnip rape, using a dCAPS primer pair, which can amplify PCR fragment only for the mutant e1 gene from A-genome. The early backcross progenies showed poor seed set, but which was improved in advanced progenies. Finally, homozygous e1e1 genotypes were established in the selfed progenies of BC2–BC3, and their LEA content was confirmed by gas-chromatography analysis. Our results and promising lines will contribute to LEA-trait selection in turnip rape and rapeseed breeding. 相似文献
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C. Ye S. Fukai I. D. Godwin H. Koh R. Reinke Y. Zhou C. Lambrides W. Jiang P. Snell E. Redoña 《Euphytica》2010,176(3):291-301
Low temperature is a major abiotic stress for rice cultivation, causing serious yield loss in many countries. To identify
QTL controlling low temperature induced spikelet sterility in rice, the progeny of F2, BC1F1 and BC2F1 populations derived
from a Reiziq × Lijiangheigu cross were exposed to 21/15°C for 15 days at the booting stage, and spikelet sterility was assessed.
For genotyping, 92 polymorphic markers from 373 SSR and 325 STS primer pairs were used. A major QTL was initially indentified
on the short arm of chromosome 10 by selective genotyping using highly tolerant and susceptible progeny from F2 and BC1F1
populations. The QTL (qLTSPKST10.1) was validated and mapped by genotyping the entire F2 (282 progeny) and BC1F1 (84 progeny)
populations. The results from the F2 population showed that qLTSPKST10.1 could explain 20.5% of the variation in spikelet
sterility caused by low temperature treatment with additive (a = 14.4) and dominant effect (d = −7.5). From the analysis of
98 selected BC2F1 progeny, the QTL located in the 3.5 cM interval between S10010.9 and S10014.4 was further confirmed. Based
on the studies of 3 generations in 2 years, it was clear that the QTL on chromosome 10 is a major determinant of the control
of low temperature induced spikelet sterility at booting stage. 相似文献
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为探究生物刺激素产品在春季花椰菜绿色防控生产中的应用效果,2019年在北京市大兴区开展4种生物刺激素对花椰菜生产的影响试验,比较植株长势、结球率、单球直径、单球质量、品质等指标,以筛选适合推广使用的生物刺激素。结果表明:苗期施用大丽轮枝孢激活蛋白(处理2)可以促进花椰菜苗期生长,推荐用量为苗期施用大丽轮枝孢激活蛋白5000倍液,定植后30 d施用12000倍液;定植后施用大丽轮枝孢激活蛋白(处理6)可以提高花椰菜的产量,推荐定植后7 d和结球前各施用1次大丽轮枝孢激活蛋白6000倍液;采用以上处理方法可以增加花椰菜的维生素C含量,提高品质和安全性。 相似文献
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Reactivity of green tea catechins with formaldehyde 总被引：3，自引：0，他引：3
Akiko Takagaki Katsuhiko Fukai Fumio Nanjo Yukihiko Hara 《Journal of Wood Science》2000,46(4):334-338
In the reaction of green tea catechins with formaldehyde at room temperature (25°C), tea catechins were found to have reactivity. In particular, (-)-epicatechin gallate and (-)-epigallocatechin gallate, which have a galloyl moiety at the C-3 position, showed higher reactivity than (+)-catechin, (-)-epicatechin, or (-)-epigallocatechin. Reactivity of various kinds of simple phenolic compounds and flavonoids with formaldehyde was also examined. Among these compounds, only phloroglucinol showed reactivity to the same degree as that of nongalloylated catechins. These results suggest that factors for reactivity with formaldehyde at room temperature may be the presence of a phloroglucinolic A-ring structure and the absence of the electron-attractive group such as a carbonyl group in Cring. The comparison of the reactivity of 3-O-acylated catechins with that of 3-O-galloylated catechins indicated that only a galloyl group effectively enhanced reactivity with formaldehyde. 相似文献
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Antinephritis activity of 5 prenylflavonoids similar to glabridin (1-5), isolated from Morus alba, Artocarpus communis, Glycyrrhiza uralensis and G. inflata, was evaluated in mice with glomerular disease (Masugi-nephritis). Oral administrations of artonin E (2) or licochalcone A (4) for 10 days (30 mg kg(-1) day(-1)) reduced the amount of urinary protein excretion compared to nephritic mice. ESR spectroscopy demonstrated that morusin (1) and licorisoflavan A (5) increased the radical intensity of sodium ascorbate by about two times. Morusin, licoricidin (3), licochalcone A and licorisoflavan A showed weak scavenging activity against superoxide anion radical. 相似文献