Vaccination of rabbits against coccidiosis using precocious lines of Eimeria magna and Eimeria media in Benin     

Akpo Y  Kpodékon MT  Djago Y  Licois D  Youssao IA 《Veterinary parasitology》2012,184(1):73-76

Three groups of twelve 35-day-old rabbits were used for the experiment. Two groups were vaccinated with a mixture of precocious lines of Eimeria magna and Eimeria media originating from corresponding wild strains isolated in Benin. One group benefited of a booster whereas the second one was kept without booster. A third non-vaccinated group was used as control. All groups were challenged per os with an equal mixture of the wild strains of E. magna and E. media at a dose of 104 oocysts per animal. Three weeks after the challenge inoculation, no case of diarrhoea was recorded in the two groups of vaccinated rabbits, as compared to the non-vaccinated rabbits that developed diarrhoea. No mortality was recorded in the three groups. During the patent period, oocyst output of vaccinated rabbits was significantly lower than that of control animals (P < 0.01), confirming a good immunogenic characteristic of the precocious lines. No booster effect was noticed for the boost vaccinated group. The daily weigh gain of the two groups of vaccinated rabbits was significantly higher than that of the non-vaccinated rabbits (P < 0.05). Consequently the precocious lines of Benin origin turned out to be immunogenic and therefore constitute good potential candidates for vaccine production for this country.  相似文献   

Genomics,genetics and breeding of tropical legumes for better livelihoods of smallholder farmers     

Chris Ojiewo  Emmanuel Monyo  Haile Desmae  Ousmane Boukar  Clare Mukankusi‐Mugisha  Mahendar Thudi  Manish K. Pandey  Rachit K. Saxena  Pooran M. Gaur  Sushil K. Chaturvedi  Asnake Fikre  NPVR Ganga Rao  CV SameerKumar  Patrick Okori  Pasupuleti Janila  Jean Claude Rubyogo  Chigeza Godfree  Essegbemon Akpo  Lucky Omoigui  Stanley Nkalubo  Berhanu Fenta  Papias Binagwa  Michael Kilango  Magdalena Williams  Omari Mponda  David Okello  Mekasha Chichaybelu  Amos Miningou  Joseph Bationo  Dramane Sako  Sory Diallo  Candidus Echekwu  Muhammad Lawan Umar  Richard Oteng‐Frimpong  Haruna Mohammed  Rajeev K. Varshney 《Plant Breeding》2019,138(4):487-499

Legumes are important components of sustainable agricultural production, food, nutrition and income systems of developing countries. In spite of their importance, legume crop production is challenged by a number of biotic (diseases and pests) and abiotic stresses (heat, frost, drought and salinity), edaphic factors (associated with soil nutrient deficits) and policy issues (where less emphasis is put on legumes compared to priority starchy staples). Significant research and development work have been done in the past decade on important grain legumes through collaborative bilateral and multilateral projects as well as the CGIAR Research Program on Grain Legumes (CRP‐GL). Through these initiatives, genomic resources and genomic tools such as draft genome sequence, resequencing data, large‐scale genomewide markers, dense genetic maps, quantitative trait loci (QTLs) and diagnostic markers have been developed for further use in multiple genetic and breeding applications. Also, these mega‐initiatives facilitated release of a number of new varieties and also dissemination of on‐the‐shelf varieties to the farmers. More efforts are needed to enhance genetic gains by reducing the time required in cultivar development through integration of genomics‐assisted breeding approaches and rapid generation advancement.  相似文献   

Market‐led options to scale up legume seeds in developing countries: Experiences from the Tropical Legumes Project     

Jean‐Claude Rubyogo  Essegbemon Akpo  Lucky Omoigui  Gaur Pooran  Sushil Kumar Chaturvedi  Asnake Fikre  Desmae Haile  Ajeigbe Hakeem  Emmanuel Monyo  Stanley Nkalubo  Berhanu Fenta  Papias Binagwa  Michael Kilango  Magdalena Williams  Omari Mponda  David Okello  Mekasha Chichaybelu  Amos Miningou  Joseph Bationo  Dramane Sako  Zoumana Kouyate  Sory Diallo  Richard Oteng‐Frimpong  Julius Yirzagla  Teryima Iorlamen  Umar Garba  Haruna Mohammed  Chris Ojiewo  Alpha Kamara  Rajeev Varshney  Shyam Narayan Nigam  Pasupuleti Janila  Hajisaheb Lalasab Nadaf  Sylvia Kalemera 《Plant Breeding》2019,138(4):474-486

There are several hurdles to ensure sustainable seed production and consistent flow of improved legume varieties in sub‐Saharan Africa (SSA) and South Asia (SA). The unreliable demand, autogamous nature of most of the grain legumes, and slow variety replacement rate by smallholder farmers do not provide strong incentive for private seed companies to invest in legume seed business. Unless a well thought‐out and comprehensive approach to legume seed delivery is developed, current seed shortages will continue, eroding emerging market opportunities. The experiences reported here are collated through a 10‐year partnership project, the Tropical Legumes in SSA and SA. It fostered innovative public–private partnerships in joint testing of innovative market‐led seed systems, skills and knowledge enhancement, de‐risking private sector initiatives that introduced in new approaches and previously overlooked entities in technology delivery. As new public and private seed companies, individual seed entrepreneurs and farmer organizations emerged, the existing ones enhanced their capacities. This resulted in significant rise in production, availability and accessibility of various seed grades of newly improved and farmer demanded legume varieties in the target countries.  相似文献   

Genetic diversity of Jatropha curcas L. in Senegal compared with exotic accessions based on microsatellite markers     

Bassiaka Ouattara  Khadidiatou Ndoye Ndir  Mame Codou Gueye  Ibrahima Diédhiou  Adeline Barnaud  Daniel Fonceka  Ndiaga Cissé  Elie Léonard Akpo  Diégane Diouf 《Genetic Resources and Crop Evolution》2014,61(6):1039-1045

Significant efforts have been undertaken in West Africa to increase biofuel production with the expectation to alleviate the dependency on fossil energies and to reduce rural poverty by diversifying cultivated crops. In this context, Jatropha curcas L., a shrub belonging to Euphorbiaceae family, has gained great interest because of its oil which can be converted to biodiesel. It is also highly adaptable to marginal soils due to its drought-tolerant characteristics. Characterisation of J. curcas germplasm in Senegal could be an important input for its better management and in identifying genotypes that could be used in breeding program. Genetic diversity of 103 accessions including 82 accessions from different agro ecological zones in Senegal and 21 exotic accessions was assessed through 33 microsatellite markers. All the markers gave amplifications at the expected band size. Only one microsatellite marker, JCT17, was polymorphic showing 3 alleles and allows distinguishing 2 accessions from Burkina Faso. The surprisingly low level of genetic variation might be because introduction of J. curcas in Senegal seems to have been done from one or a few origins and the species has not regained genetic diversity since then due to vegetative propagation. Cultivation of J. curcas at large scale may face to vulnerability to pests and require many cautions. They are necessity to widen the genetic base of J. curcas in Senegal via new introductions from its centre of origin.  相似文献