Using the indicator amino acid oxidation technique to study threonine requirements in horses receiving a predominantly forage diet          下载免费PDF全文

Chan Hee Mok  Crystal L. Levesque  Kristine L. Urschel 《Journal of animal physiology and animal nutrition》2018,102(5):1366-1381

Threonine has been reported to be the second limiting amino acid in typical equine diets, but its actual requirement has not been determined in horses. To evaluate amino acid metabolism and requirements, the indicator amino acid oxidation (IAAO) method has been successfully used in other species. The objective of this research was to estimate threonine requirements in mature horses fed timothy hay and concentrate in 4:1 ratio using the IAAO method. Six Thoroughbred mares (579.9 ± 46.7 kg) received each of 6 levels of threonine intake, 41, 51, 61, 70, 80 and 89 mg/kg BW/day, in a randomly determined order. Each study period was 7‐day long, and on day 6, blood samples were collected before and 90 min after feeding to measure amino acid concentrations using HPLC. On day 7, horses underwent IAAO procedures, which included a 2‐hr primed, constant intravenous infusion of [¹³C]sodium bicarbonate to measure total CO₂ production and a 4‐hr primed, constant oral administration of [1‐¹³C]phenylalanine to estimate phenylalanine oxidation to CO₂. Blood and breath samples were collected to measure blood [¹³C]phenylalanine, using GC‐MS analysis and breath ¹³CO₂ enrichment, using an infrared isotope analyser. Increasing threonine intake levels did not affect plasma phenylalanine oxidation by the ANOVA test (p > 0.05) but resulted in a linear decrease in phenylalanine oxidation (p = 0.04) without a breakpoint by the orthogonal linear contrast. This study is the first attempt to evaluate threonine requirements in horses by the IAAO method; however, threonine requirements are still unknown in mature horses at this time.  相似文献   

In vitro bactericidal activity of 465–470 nm blue light phototherapy and aminolevulinic acid on Staphylococcus pseudintermedius          下载免费PDF全文

Seulgi Bae  Taeho Oh 《Veterinary dermatology》2018,29(4):296-e102

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Rare β-Resorcylic Acid Derivatives from a Halophyte-Associated Fungus Colletotrichum gloeosporioides JS0419 and Their Antifungal Activities     

Sunghee Bang  Jaekyeong Kim  Jiwon Oh  Ji-Seok Kim  Seong-Ryong Yu  Stephen Deyrup  Yong-Sun Bahn  Sang Hee Shim 《Marine drugs》2022,20(3)

Six new β-resorcylic acid derivatives (1–5 and 7) were isolated from a halophyte-associated fungus, Colletotrichum gloeosporioides JS0419, together with four previously reported β-resorcylic acid lactones (RALs). The relative and absolute stereochemistry of 1 was completely established by a combination of spectroscopic data and chemical reactions. The structures of the isolated compounds were elucidated by analysis of HRMS and NMR data. Notably, compounds 1–3 had a β-resorcylic acid harboring a long unesterified aliphatic side chain, whereas the long aliphatic chains were esterified to form macrolactones in 4–9. Among the isolated compounds, monocillin I and radicicol showed potent antifungal activities against Cryptococcus neoformans, comparable to clinically available antifungal agents and radicicol showed weak antifungal activity against Candida albicans. These findings provide insight into the chemical diversity of fungal RAL-type compounds and their pharmacological potential.  相似文献   

Improvement of water-stability of clay aggregates admixed with aqueous polymer soil stabilizers     

Jin Liu  Bin Shi  Hongtao Jiang  Sunyoung Bae  He Huang 《CATENA》2009,77(3):175-179

Aqueous soil stabilizers improve soil properties. Water-stability of soil aggregates was determined using two different soil stabilizers: S-type and E-type. Various concentrations of the two soil stabilizers were tested in soil aggregates that were bigger than 5 mm but smaller than 10 mm. Testing the aggregates using the static water-measure method showed that the water-stability index, K, increased with the concentrations of soil stabilizers. S-type soil stabilizer in concentrations ranging between 20% and 40% resulted in K-values between 64.0% and 83.8%, and E-type polymer emulsions in concentrations between 3% and 7% resulted in K-values between 90.1% and 99.7% respectively. The soil aggregates admixed with water resulted in a K-value of 11.5%, which was tested as a control. Two aggregate collapse models were found and discussed based on the collapse characteristics of the modified aggregates: S-type soil stabilizer in low concentrations follows a gradual collapse pattern, while S-type stabilizer in high concentrations and E-type stabilizer in low concentrations follow an explosive collapse pattern. These patterns depend on the formation of a membrane structure on the surface of the aggregates through physicochemical bonding. By identifying the mechanisms of the membrane structure and calculating the K-values, significant information on the improvement of soil properties can be determined.  相似文献   

Application of the rpoS gene for the detection of Vibrio anguillarum in flounder and prawn by polymerase chain reaction     

Kim DG  Bae JY  Hong GE  Min MK  Kim JK  Kong IS 《Journal of fish diseases》2008,31(9):639-647

Vibrio anguillarum , an opportunistic fish pathogen, is the main species responsible for vibriosis, a disease that affects feral and farmed fish and shellfish, and causes considerable economic losses in marine aquaculture. In this study, we used polymerase chain reaction (PCR) to detect V. anguillarum . PCR specificity was evaluated by amplifying the rpoS gene, a general stress regulator, in six strains of V. anguillarum and 36 other bacterial species. PCR amplified a species-specific fragment (689 bp) from V. anguillarum . Furthermore, the PCR assay was sensitive enough to detect rpoS expression from 3 pg of genomic DNA , or from six colony-forming units (CFU) mL⁻¹ of cultured V. anguillarum . However, the assay was less sensitive when genomic DNA from the infected flounder and prawn was used (limit of detection, 50 ng and 10 ng g⁻¹ tissue, respectively). These data demonstrate that PCR amplification of the rpoS gene is a sensitive and species-specific method to detect V. anguillarum in practical situations.  相似文献   

Numerical analysis of injection molding for the Syringe Barrel with optimum design and processing condition     

Du Hwan Chun 《Fibers and Polymers》2017,18(9):1790-1795

The numerical analysis was performed to predict the potential problem, often occurring during the manufacturing process of the disposable medical device of a great volume. The cavity filling analyses were performed for the new design of the 3cc Syringe Barrel using polypropylene(PP1), and a new nucleated polypropylene(PP2) material for better clarity. These analyses have been performed for different processing conditions as well as various wall thickness designs for both materials. With the nucleated material, only the original wall thickness design has been studied at two different processing conditions for comparison purpose. This study was conducted to investigate the optimum part design and processing condition for two different materials. The most desirable design was selected with Design 3 for material utilization and reduced flow stresses by comparing the field results. The new nucleated polypropylene provided slightly better product quality and processing.  相似文献   

Cellular Localization of Rice SUMO/SUMO Conjugates and in vitro Sumoylation Using Rice Components     

Joo Joungsu  Hee Choi Dong  Hyon Kim Sang  Ik Song Sang 《水稻科学》2020,27(1):1-4

正Many proteins are regulated by post-translational modifications,such as the reversible covalent attachment of ubiquitin and ubiquitin-like proteins in eukaryotes(Kerscher et al, 2006). Post-translational modification of proteins by the SUMO protein family is involved in diverse cellular processes, including development, hormonal responses, and biotic and abiotic stress signaling(Park et al, 2011). SUMO modification can modulate protein-protein interactions, intracellular localization or the activities of the protein(Gareau and Lima, 2010).  相似文献   

Compensatory growth and body composition of juvenile black rockfish <Emphasis Type="Italic">Sebastes schlegeli</Emphasis> following feed deprivation   总被引：1，自引：0，他引：1  

Sung-Yong Oh  Choong Hwan Noh  Rae-Seon Kang  Chong-Kwan Kim  Sung Hwoan Cho  Jae-Yoon Jo 《Fisheries Science》2008,74(4):846-852

ABSTRACT:   Compensatory growth, feeding rate, feed efficiency and chemical composition of juvenile black rockfish (mean weight 1.43 g) were investigated for 35 days after a 14-day feed deprivation treatment under four feeding conditions: one group continuously fed (control) and the other three groups fasted for 5 days (F5), 10 days (F10) and 14 days (F14). All fasted fish were re-fed from day 15. Only F5 achieved the same body weight as the control, indicating that complete compensation occurred in F5. The specific growth rate (SGR) of F5 was the highest at day 21 and then decreased thereafter, showing higher values than the control at days 21, 28 and 42. In contrast, although SGRs of F10 and F14 were higher than that of the control during the whole refeeding period except day 21, they did not catch up the control in body mass, indicating that only partial compensation occurred in F10 and F14. The feeding rate (FR) of all groups except F14 changed in a pattern similar to SGR (Spearman's rank correlation, r _s > 0.9), suggesting that SGR varied depending on FR. Similar feeding efficiencies (FEs) were found in the four groups and they did not vary significantly during the whole refeeding period, suggesting that FE was not the factor affecting SGR. At day 14, the ratios of lipid to lean body mass in F10 and F14 were lower than those in the control and F5, and there was no difference between the control and F5. At day 49, however, only F14 showed a lower value than the other three groups, and there was no difference among the three groups. These results indicate that juvenile black rockfish fasted for 5–14 days can exhibit compensatory growth after refeeding, but timing and degree vary depending on the duration of feed deprivation.  相似文献   

Genetic Diversity and Population Structure in the Heavily Exploited Korean Rockfish,Sebastes schlegeli,in Korea     

Hyesuck An  Mi‐Jung Kim  Kwangjae Park  Keechae Cho  Bongseong Bae  Jaewoo Kim  Jeong‐In Myeong 《Journal of the World Aquaculture Society》2012,43(1):73-83

The Korean rockfish, Sebastes schlegeli, is a valuable and intensively exploited species in Korea. We discuss the genetic diversity and genetic structure of four Korean rockfish populations using eight microsatellite loci. In total, 161 different alleles from 138 individuals were observed. Average allele number per locus ranged from 2.5 to 23 and allelic richness varied from 13.38 to 14.63 within a population. Despite a long history of stocking practices, we found very high levels of polymorphism (mean heterozygosity = 0.810), which is comparable to other congeneric species. No significant difference in genetic diversity and molecular genetic variance (F_ST and R_ST) was observed among four local samples (P > 0.1). Little indication of contemporary inbreeding (F_IS= 0.051) or population structure (K = 1) was detected. This absence of differentiation may reflect high levels of gene flow along the coast of Korea. Our study demonstrates that rockfish in Korea should be managed as a single unit. Currently, the species does not appear to be genetically threatened, but the potential for a rapid loss of genetic diversity remains. This information on the genetic characteristics of Korean rockfish populations has important implications for fisheries management and conservation efforts, and will aid in the sustainable exploitation of the fishing resources and the preservation of biodiversity.  相似文献   

Guided bone regeneration with beta-tricalcium phosphate and poly L-lactide-co-glycolide-co-epsilon-caprolactone membrane in partial defects of canine humerus     

Taehoon Oh  Md. Mizanur Rahman  Ji-Hey Lim  Mi-Sun Park  Dae-Yong Kim  Jung-hee Yoon  Wan Hee Kim  Masanori Kikuchi  Junzo Tanaka  Yoshihisa Koyama  Oh-Kyeong Kweon 《Journal of veterinary science (Suw?n-si, Korea)》2006,7(1):73-77

This study was performed to evaluate the effect of beta-tricalcium phosphate and poly L-lactide-co-glycolide-co-epsilon-caprolactone (TCP/PLGC) membrane in the repair of partial bone defects in canine proximal humerus. Three adult mixed-breed dogs were used during the experimental period. The length of the defect was quarter of the full length of humerus, and width of the defect was quarter of middle diameter of the lateral aspect of humerus. The humeri of each dog were divided into treatment (TCP/PLGC) and control groups. The defect was covered with TCP/PLGC membrane in treatment group. To evaluate regeneration of the bone, computerized tomography (CT) and histopathologic examination were performed. The radiopaque lines were appeared at the original defect sites in TCP/PLGC group but below the original site in control at 4th week. Radiopacity and thickness of the defect sites, and radiopaque lines were more increased at 8th week than those of 4th week. Histopathologic findings revealed fibrous connective tissue migration into the defect and the migration inhibited the structure of new cortex to be placed in the original level in control whereas new cortex growth was found in the level of original line in TCP/PLGC group. However, the new cortical bone in the TCP/PLGC group was thinner and less organized than the adjacent intact cortex, and the amount of new cancellous bones were also scanty. The result suggested that TCP/PLGC membrane is a good guided bone regeneration material to restore the original morphology of humerus in partial defect.  相似文献