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1.
试验旨在探讨解耦联蛋白1（UCP1）基因核苷酸变异对绵羊生长性状的性别差异和胴体性状的影响，以期能够筛选出可以提升绵羊生长及胴体性状的核苷酸变异，为提高绵羊相关重要经济性状的分子遗传标记提供材料。以9个绵羊品种为研究对象，用PCR-SSCP方法检测不同性别绵羊中UCP1基因内含子5区和外显子6区变异。利用Minitab 16.0软件中一般线性模型分析内含子5区等位基因与不同性别绵羊生长性状、公羔胴体性状的关联性。结果显示，绵羊UCP1基因内含子5区和外显子6区共检测到8个核苷酸变异，其中位点c.910 G/A突变导致p.Ala304Thr氨基酸变异。生长性状关联分析结果表明，内含子5区等位基因对绵羊生长性状的影响存在性别特异性，母羔中携带等位基因A1的群体较缺失群体具有较低的初生重（P<0.05），公羔中携带等位基因C1的群体较缺失群体具有较高的断尾重（P<0.05），未发现其他等位基因与羔羊的生长性状存在性别特异性。胴体性状关联分析结果表明，携带等位基因A1的群体具有较低后腿瘦肉量、腰部瘦肉量和较高的肩部瘦肉比例（P<0.05），携带等位基因C1的群体具有较低的后腿瘦肉比例（P<0.05），其他胴体性状均没有发现与等位基因存在显著相关，基因型分析结果与等位基因分析结果一致。结果提示，UCP1基因对绵羊的生长性状影响具有性别特异性，且携带等位基因A1的公羔群体具有较低的胴体生产性状，为提高公羔胴体生产性能提供了理论依据。 相似文献
2.
MARC R. RAFFE dvm ms Diplomateacva STEPHEN I. BISTNER dvm Diplomateacvo ANTHONY J. CRIMI JON RUFF 《Veterinary surgery : VS》1986,15(1):139-141
The use of retrobulbar block in conjunction with general anesthesia was evaluated in the horse. A prospective study of 12 cases fulfilling criteria for application of retrobulbar block was compared to a retrospective survey of 25 cases (control group) in which retrobulbar block was not performed. Retrobulbar block was done using a modified Peterson technique and 2% lidocaine HCI without epinephrine. In the control group, four horses exhibited signs compatible with initiation of the oculocardiac reflex; three horses developed bradyarrhythmias and hypotension during global traction related to enucleation, and cardiac arrest occurred in one horse during cataract extraction. In the retrobulbar block group, one horse developed bradyarrhythmia subsequent to retrobulbar block. 相似文献
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MILA FREIRE IAN ROBERTSON HOWARD D. BONDELL JAMES BROWN JON HASH ANTHONY P. PEASE B. DUNCAN X. LASCELLES 《Veterinary radiology & ultrasound》2011,52(3):239-247
Degenerative joint disease (DJD) is common in domesticated cats. Our purpose was to describe how radiographic findings thought to indicate feline DJD relate to macroscopic cartilage degeneration in appendicular joints. Thirty adult cats euthanized for reasons unrelated to this study were evaluated. Orthogonal digital radiographs of the elbow, tarsus, stifle, and coxofemoral joints were evaluated for the presence of DJD. The same joints were dissected for visual inspection of changes indicative of DJD and macroscopic cartilage damage was graded using a Total Cartilage Damage Score. When considering all joints, there was statistically significant fair correlation between cartilage damage and the presence of osteophytes and joint‐associated mineralizations, and the subjective radiographic DJD score. Most correlations were statistically significant when looking at the different joints individually, but only the correlation between the presence of osteophytes and the subjective radiographic DJD score with the presence of cartilage damage in the elbow and coxofemoral joints had a value above 0.4 (moderate correlation). The joints most likely to have cartilage damage without radiographic evidence of DJD are the stifle (71% of radiographically normal joints) followed by the coxofemoral joint (57%), elbow (57%), and tarsal joint (46%). Our data support radiographic findings not relating well to cartilage degeneration, and that other modalities should be evaluated to aid in making a diagnosis of feline DJD. 相似文献
4.
KAREN J. WHITEHAIR DVM MS STEPHEN B. ADAMS DVM MS Diplomate ACVS JAMES P. TOOMBS DVM MS Diplomate ACVS JILL E. PARKER VMD Diplomate ACVS JOSEPH M. PROSTREDNY DVM MS JON G. WHITEHAIR DVM SEAN W. AIKEN DVM 《Veterinary surgery : VS》1992,21(3):228-233
A llama, a miniature horse, and a miniature donkey with severe bilateral congenital flexural deformities of the metacarpophalangeal and metatarsophalangeal joints were treated successfully by arthrodesis with dynamic compression plating or external skeletal fixation. The flexor tendons were more taut than the suspensory ligaments and were transected. In the llama, the suspensory ligament was transected, and overcorrection caused ischemic necrosis of one distal limb and subluxation of the other fetlock joint. In the horse and donkey, the suspensory ligament was preserved and wedge osteotomy was performed to correct the deformity. 相似文献
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DANIEL A. DEGNER DVM RICHARD WALSHAW BVMS Diplomate ACVS STEVE P. ARNOCZKY DVM MS Diplomate ACVS RICK J. SMITH MD JON S. PATTERSON DVM PhD Diplomate ACVP LISA A. DEGNER DVM ANNICK HAMAIDE DVM DIANA ROSENSTEIN DVM 《Veterinary surgery : VS》1996,25(4):292-299
This study evaluates the cranial rectus abdominus muscle pedicle flap as the sole blood supply for the caudal superficial epigastric skin flap. This flap was composed of a cranially based rectus abdominus muscle pedicle flap that was attached to the caudal superficial epigastric island skin flap (including mammary glands 2 to 5) via the pudendoepigastric trunk. Selective angiography of the cranial epigastric artery in eight cadaver dogs proved that the arterial vasculature in the cranial rectus abdominus was contiguous with that in the caudal superficial epigastric skin flap. In the live dog study, three of six of the flaps failed because of venous insufficiency. Necrosis of mammary gland 2 occurred in two of six flaps. One of six flaps survived with the exception of the cranial most aspect of mammary gland 2. Angiography of the cranial epigastric artery proved that arterial blood supply to these flaps was intact. Histological evaluation of the failed flaps showed full-thickness necrosis of the skin and subcutaneous tissues, the presence of severe congestion, and venous thrombosis. Retrograde venous blood flow through the flap was inconsistent, and hence resulted in failure of this myocutaneous flap. Use of this flap for clinical wound reconstruction cannot be recommended. 相似文献
7.
KIRK WENDELBURG DVM JON DEE DVM MS DipiomateACVS ROBERT KADERLY DVM PhD DipiomateACVS LARRY DEE DVM RICHARD EATON-WELLS BVSC 《Veterinary surgery : VS》1988,17(3):128-134
Acetabular fractures in 26 racing Greyhounds were reviewed. All fractures occurred during racing or training and were unrelated to any external trauma. All fractures had similar configurations, were minimally displaced, and involved only the acetabulum. Affected dogs were young (16-36 months). Fractures occurred unilaterally (22 dogs) and bilaterally (4 dogs). There was no sex predilection, and both right and left sides were equally represented. Bilateral fractures were associated with retraining between the occurrence of the first and second fractures. Radiographically, a fracture line was consistently visible in the caudal third of the acetabulum. Fractures in gross specimens resembled an inverted "Y." Results of histologic evaluation of two fractured acetabula showed changes characteristic of a nonunion fracture. Microfractures were evident in the grossly normal acetabulum opposite the fractured side. Evidence obtained from this study suggests a common pathogenesis of the fractures related to tremendous repetitive stresses produced during running. Greyhounds with unilateral acetabular fractures may provide a reproducible model for future studies of stress fractures in animals and humans because of the high incidence of bilateral fractures that develop during retraining. Surgical repair of the acetabular stress fracture was more successful than conservative management in returning the dogs to competitive racing. 相似文献
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ROLFE M. RADCLIFFE DVM Diplomate ACVS JON CHEETHAM Vet MB Diplomate ACVS ABRAHAM J. BEZUIDENHOUT BVSc DVSc NORM G. DUCHARME DVM MS Diplomate ACVS ALAN J. NIXON BVSC MS Diplomate ACVS 《Veterinary surgery : VS》2008,37(8):733-740
Objective— To describe anatomic considerations and arthroscopic technique in horses for arthroscopic removal of palmar/plantar osteochondral fragments from the proximal interphalangeal (PIP) joint. Study Design— Retrospective study. Animals— Adult horses (n=4) with osteochondral fragments of the palmar/plantar PIP joint. Methods— Arthroscopic removal of palmar/plantar osteochondral fragments within the PIP joint was performed with horses in dorsal recumbency under general anesthesia. Medical records of affected horses were reviewed to determine history; physical, lameness, and radiological findings; surgical technique; complications and outcome. Results— Two horses had lameness localized to the PIP joint. Two other horses had lameness suspected, but not confirmed to the pastern region. One of these horses had a history of intermittent lameness, but was not lame on admission. All horses had radiographic evidence of palmar/plantar osteochondral fragmentation within the PIP joint. Fragmentation was located abaxially in 2 horses in the hind limb and axially in 2 horses in the left forelimb. Osteochondral fragments were successfully removed via a palmar/plantar arthroscopic approach in all horses. Three horses returned to previous levels of athletic performance; 1 horse was used for trail riding instead of reining. Conclusions— Arthroscopy of the palmar/plantar pouch of the PIP joint allowed limited assessment of the joint and removal of osteochondral fragments. Clinical Relevance— Arthroscopy of the palmar/plantar PIP joint pouch for assessment and removal of osteochondral fragments is possible and should be considered when lameness is localized to this joint. 相似文献
9.
The Accuracy of Intraoperative Diagnoses Based on Examination of Frozen Sections A Prospective Comparison with Paraffin-Embedded Sections 总被引：1，自引：1，他引：0
JON G. WHITEHAIR dvm STEPHEN M. GRIFFEY dvm HARVEY J. OLANDER dvm PhD Dipiomate acvp PHILIP B. VASSEUR dvm Dipiomate acvs DIANE NAYDAN as ht 《Veterinary surgery : VS》1993,22(4):255-259
The accuracy of diagnoses based on examination of frozen sections was determined by comparing the results to those obtained by examination of tissues prepared using conventional methods (formalin fixation, paraffin-embedded tissue). One hundred ninety-four specimens were examined using the frozen section technique; 37 were examined to confirm a tentative diagnosis or to document lymph node metastasis and the remainder were examined to diagnose an unknown pathologic process. Of the 194 specimens examined, an accurate, specific diagnosis was obtained in 161 (83%); in 19 (10%), the pathologic process was correctly identified, but a specific diagnosis was not obtained; and in 2 (1%) the diagnosis was deferred. The remaining 12 (6%) were incorrectly diagnosed by the frozen section technique. When the number of specimens in which a specific diagnosis was obtained was combined with the number of specimens in which the pathologic process was correctly identified, the overall accuracy rate of the frozen section technique was 93%. There was no difference in the accuracy of the frozen section technique based on the reason for submission of the sample, source of tissue submitted, or the type of pathologic process (i.e., inflammatory or neoplastic). Of the 12 incorrect diagnoses, 4 (33%) were because of sampling errors and 8 (67%) were caused by interpretation errors. The proposed indications for the use of intraoperative frozen sections are: 1) to determine the nature of a pathologic process for which a preoperative diagnosis has not been established, 2) to determine the extent of spread of neoplastic tissue to lymph nodes and other organs, 3) to evaluate resection margins of a neoplastic process, and 4) to clarify situations where a discrepancy exists between the preoperative cytologic or histologic diagnosis and intraoperative gross pathology. 相似文献
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1. The effects of macrophyte removal from a regulated reach of the River Great Ouse, UK, were recorded. The relationship between macrophyte cover, zooplankton distribution and 0+roach Rutilus rutilus (L.) distribution, diet and growth, were compared before, directly after, and over several weeks following weed-cutting operations. 2. Both zooplankton and fish were significantly associated with the macrophyte zone before weed cutting. It is suggested that this zone provides high food densities and refuge during periods of elevated flows. 3. Removal of all but a 2 m marginal strip of the macrophyte zone led to a rapid decline in the mean densities of planktonic Cladocera, probably as a result of increased washout, fish predation and starvation. This was accompanied by a rapid decline in the growth rate of the roach, which were forced to feed on the less nutritious aufwuchs. 4. It is suggested that river management involving weed cutting adjacent to one bank in alternate years would be sufficient to prevent loss of channel capacity and would provide a refuge for fish and zooplankton. 相似文献