Effect of different planting times on the quantitative variation of total seed isoflavone content and composition in Korean soybean cultivars (Glycine max (L.) Merr.)     

Kim  Hong-Sik  Kang  Beom-Kyu  Seo  Jeong-Hyun  Kim  Hyun-Tae  Ha  Tae-Joung  Oh  Jae-Hyeon  Shin  Sang-Ouk  Baek  In-Yeol 《Journal of Crop Science and Biotechnology》2021,24(2):179-190

Journal of Crop Science and Biotechnology - There is great interest in the enhancement of isoflavones as one of the functional ingredients in soybean. This study aimed to investigate the effects of...  相似文献   

Differential regulation of senescence and in vitro differentiation by 17β-estradiol between mesenchymal stem cells derived from male and female mini-pigs     

Won-Jae Lee  Seung-Chan Lee  Jeong-Hyun Lee  Gyu-Jin Rho  Sung-Lim Lee 《Journal of veterinary science (Suw?n-si, Korea)》2016,17(2):159-170

The characterization and potential of mesenchymal stem cells (MSCs) are gender dependent and estrogen influences these properties. This study demonstrated that supplementation with 17β-estradiol (E2) increases the proliferation of bone marrow-MSCs derived from male and female mini-pigs (Mp- and Fp-BMSCs) in a concentration-dependent manner, with 10^-12 M E2 suggested as the optimal dose of E2 that led to the greatest improvement in BMSCs proliferation. Supplementation of 10^-12 M E2 resulted in down-regulation of β-galactosidase activity and pro-apoptotic activity in both BMSCs, while anti-apoptotic activity was up-regulated in only Fp-BMSCs. Further, E2 increased the osteogenic ability of Fp-BMSCs. Based on these findings, optimal utilization of E2 can improve cellular senescence and apoptosis, as well as in vitro osteogenesis of BMSCs, and could therefore be useful in stem cell therapy, particularly in bone regeneration for adult females.  相似文献   

Dieckol Ameliorates Aβ Production via PI3K/Akt/GSK-3β Regulated APP Processing in SweAPP N2a Cell     

Jeong-Hyun Yoon  Nayoung Lee  Kumju Youn  Mi Ra Jo  Hyeung-Rak Kim  Dong-Seok Lee  Chi-Tang Ho  Mira Jun 《Marine drugs》2021,19(3)

The proteolytic processing of amyloid precursor protein (APP) by β-secretase (BACE1) and γ-secretase releases amyloid-β peptide (Aβ), which deposits in amyloid plaques and contributes to the initial causative events of Alzheimer’s disease (AD). In the present study, the regulatory mechanism of APP processing of three phlorotannins was elucidated in Swedish mutant APP overexpressed N2a (SweAPP N2a) cells. Among the tested compounds, dieckol exhibited the highest inhibitory effect on both intra- and extracellular Aβ accumulation. In addition, dieckol regulated the APP processing enzymes, such as α-secretase (ADAM10), β-secretase, and γ-secretase, presenilin-1 (PS1), and their proteolytic products, sAPPα and sAPPβ, implying that the compound acts on both the amyloidogenic and non-amyloidogenic pathways. In addition, dieckol increased the phosphorylation of protein kinase B (Akt) at Ser473 and GSK-3β at Ser9, suggesting dieckol induced the activation of Akt, which phosphorylated GSK-3β. The specific phosphatidylinositol 3-kinase (PI3K) inhibitor LY294002 triggered GSK-3β activation and Aβ expression. In addition, co-treatment with LY294002 noticeably blocked the effect of dieckol on Aβ production, demonstrating that dieckol promoted the PI3K/Akt signaling pathway, which in turn inactivated GSK-3β, resulting in the reduction in Aβ levels.  相似文献   

Quantitative trait loci mapping for flooding tolerance at an early growth stage of soybean recombinant inbred line population     

Sanjeev K. Dhungana  Hong-Sik Kim  Beom-Kyu Kang  Jeong-Hyun Seo  Hyun-Tae Kim  Sang-Ouk Shin  Chang-Hwan Park  Do-Yeon Kwak 《Plant Breeding》2020,139(3):626-638

Flooding stress causes a significant yield reduction in soybean. The early growth of soybean in Korea coincides with the rainy season, potentially exposing to flooding stress. Greenhouse experiments were conducted to map the quantitative trait loci (QTL) for flooding tolerance in soybean and to identify and investigate candidate genes near the QTL hot spots. Flood stress was imposed at V1–V2 stage on a recombinant inbred line population (‘Paldalkong’ × ‘NTS1116’), and leaf chlorophyll content (CC) and shoot dry weight (DW) were measured under control and flooded conditions. The genetic map was constructed using 180K Axiom^® SoyaSNP markers. The QTL were analysed under control and flooded conditions as well as for index (ratio of CC or DW under flooded to control, CCI and DWI) and flooding tolerance index (FTI, mean of CCI and DWI). A total of 20 QTL with LOD scores 3.59–19.73 causing 5.8%–33.3% phenotypic variation were identified on nine chromosomes. Chromosomes 10, 12 and 13 harboured relatively more stable QTL. Results of this study could be useful to further understand the genetic basis of soybean's flooding tolerance and applied in breeding programmes.  相似文献   

Analysis of differentially expressed genes in soybean leaf tissue of tolerant and susceptible cultivars under flooding stress revealed by RNA sequencing     

Dhungana  Sanjeev K.  Kim  Hong-Sik  Kang  Beom-Kyu  Seo  Jeong-Hyun  Kim  Hyun-Tae  Oh  Jae-Hyeon  Shin  Sang-Ouk  Baek  In-Yeol 《Journal of Crop Science and Biotechnology》2021,24(1):83-91

Journal of Crop Science and Biotechnology - Flooding stress causes severe yield reduction in soybean worldwide. The development of stress-tolerant cultivars could be an effective measure to reduce...  相似文献