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Md. Taslim Anwar Athar Imam Khan Sivasankaran Balasubramanian Ramamurthy Jayaprakash Thandavan A. Kannan Sakthivel Manokaran Sathiamangalam A. Asokan Chitravelan Veerapandian 《The Canadian veterinary journal. La revue veterinaire canadienne》2009,50(8):854-856
We present a case of globosus amorphus delivered from a goat and subjected to radiography and histological examination. Radiography revealed a lack of development of any organ system; histological sections showed evidence of lymphoid aggregations, mononuclear infiltrations, blood capillaries, and dense fibroblasts. 相似文献
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Two n-type conjugated polymers of pyrazine and quinoxaline containing poly(p-phenylene vinylene) (P-1) (P-2) have been synthesized through Wittig route. Formation of the polymers were confirmed by spectral (UV-Vis, PL, FT-IR and NMR), elemental and GPC analysis. These polymers were soluble in common organic solvents such as THF, CH2Cl2, CHCl3 and CH3CN. The resulting polymers have good thermal stability upto 308 and 361 °C with the 5 % weight loss. The absorption spectrum of the polymers displayed the maximum at 398 and 414 nm, corresponding to the ??-??* electronic transition of the conjugated polymer backbones. The optical band gap of these polymers found to have in the range of 2.5?C2.53 eV. The photoluminescence spectrum of the polymers was observed at 487 and 505 nm in CHCl3 solution. AFM images depicted that the micrometer sized globular and triangular shaped particles can be seen from the polymer surfaces. 相似文献
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A. Kumar J. Sharma V. Munjal K. Sakthivel S. K. Thalor K. K. Mondal S. Chinchure R. Gharate 《Plant pathology》2020,69(2):347-359
Yellow-pigmented bacteria isolated from blight-affected pomegranate leaves and fruit across seven Indian states in epidemics during the years 2008–2016 were characterized and identified using phenotypic and genotypic tools. All bacterial isolates shared phenotypic traits such as colony morphology, NaCl and pH sensitivity and fuscan production, and caused typical lesions on pomegranate plants upon artificial inoculation. Analysis of 16S ribosomal DNA and 16S–23S rDNA intergenic spacer sequences confirmed their identity as Xanthomonas axonopodis pv. punicae. The new isolates collected after 2000 were compared with an old isolate from the 1950s using polyphasic taxonomic approaches including multilocus sequence analysis (MLSA). Nucleotide polymorphism in 24 isolates for nine genomic loci (dnaK, fyuA, gyrB (Young), gyrB (Almeida), rpoD, fusA, gapA, gltA and lepA) showed minor variations in loci fyuA and gyrB. Isolates were grouped into four nearly identical sequence types, ST1, ST2, ST3 and ST4, based on their allelic profiles, ST3 being widespread in Indian states. Molecular phylogenetic analysis of concatenated 5690 bp with other Xanthomonas pathovars revealed its close genetic similarity with the X. citri group. The blight outbreak in diverse geographical locations is attributed to a re-emerged clonal population of X. axonopodis pv. punicae on a genetically homogenous pomegranate cultivar. The latently infected vegetative planting material of elite pomegranate cultivars contributed to the dissemination of the bacterial inoculum. This study highlights and forewarns of the role played by the clonally propagated elite pomegranate cultivars in disseminating and sustaining clonal populations of this bacterial plant pathogen in many Indian states. 相似文献
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Passoupathy Rajendrakumar Akshaya Kumar Biswal Kannabiran Sakthivel Maganti Sheshu Madhav Chirravuri Neeraja Sena M. Balachandran Kommoju Srinivasarao Podishetty Natarajkumar Yadla Hari Kalidindi Sujatha Raman M. Sundaram 《Euphytica》2009,169(2):263-271
SSR markers targeting (GATA)
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motifs are known to be highly polymorphic and useful in many organisms. (GATA)
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motif specific SSR markers covering the whole rice genome are not available. The present study was carried out with an objective
to identify class I rice microsatellites in the rice genome with (GATA)n motifs, in-silico, and validate their potential as molecular markers. A total of 243 such motifs were identified; 65 of these
were present in the genic region, 59 were in the upstream region and the remaining motifs were found in the intergenic regions.
Many of the (GATA)
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motifs were found within and/or upstream of genes associated with biotic or abiotic stress tolerance. A total of 230 PCR-based
markers targeting all the class I (GATA)
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microsatellites were developed and 35 of these markers spread across the rice genome were validated in a set of 24 representative
rice varieties belonging to five distinct cultivar groups. All the markers were polymorphic, with average polymorphism information
content (PIC) value of 0.61, and the rice cultivars could be uniquely distinguished into different cultivar groups based on
marker analysis. These informative markers targeting (GATA)
n
motifs representing a new set of markers in rice will be highly useful for genetic studies and marker-assisted selection.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users.
Passoupathy Rajendrakumar, Akshaya Kumar Biswal and Kannabiran Sakthivel contributed equally. 相似文献
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Alterations in inheritance pattern and level of cytosine DNA methylation,and their relationship with heterosis in rice 总被引：2，自引：0，他引：2
K. Sakthivel K. Girishkumar G. Ramkumar V. V. Shenoy S. T. Kajjidoni P. M. Salimath 《Euphytica》2010,175(3):303-314
Novel hybrid-specific/heterotic gene expression patterns observed from expression studies suggest the need to characterize
the underlying regulatory mechanism(s) to reveal the biological basis of heterosis in crop plants. To gain an insight into
the molecular basis of heterosis in rice, we investigated the inheritance pattern and level of cytosine methylation, a major
epigenetic regulatory mechanism, in the leaf tissue of an elite Indian rice hybrid KRH2 and its parents at three stages (15 day-old,
35 day-old seedling and flag leaf) and their correlation with heterosis using methylation-sensitive amplification polymorphism
(MSAP) technique. In contrast to parents, the level of methylation was high during initial growth stages and reduced as the
hybrid grew. Even though, a majority of cytosine methylation profiles were transmitted to hybrid at all stages, a considerable
level (25.4%) of cytosine methylation pattern was observed to be novel in hybrid and some of these altered loci were identified
to code for known/hypothetical proteins. It was also observed that demethylation events occurred more predominantly during
early stages than hypermethylation while the frequency of demethylation events decreased in flag leaf with a remarkable increase
in hypermethylation in the hybrid. These bi-directional events in methylation in KRH2 were validated in six hybrids with different
levels of grain yield heterosis and the level of cytosine methylation in the hybrid was observed to be significantly correlated
with heterosis. 相似文献
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Muthusamy Ranjithkumar Tauseef Ahmed Malik Anju Saxena Ananya Dan Pillanatham Civalingam Sakthivel Sahadeb Dey 《Tropical animal health and production》2013,45(2):417-421
Trypanosomiasis caused by Trypanosoma evansi commonly produces wasting disease with signs of emaciation and cachexia mainly at the end stage. The present study was conducted to explore the possible hyperlipaemia or hyperlipidaemia and its association with cachexia–anorexia in equine trypanosomiasis. Out of the fifteen confirmed animals, none of the plasma sample was opaque. There was a significant increase in plasma triglyceride, total cholesterol and blood urea nitrogen and a highly significant increase in low-density lipoprotein (LDL) levels. A mild increase in high-density lipoprotein (HDL) and very low-density lipoprotein levels were observed, while the relative percentage of HDL and LDL was altered with high significance. A moderate increase in triglyceride and highly significant increase in LDL might be the reasons for retention of appetite and lipolysis. Possible protein breakdown and presence of lipolysis might be the reasons for cachexia in equine trypanosomiasis. 相似文献
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I. Jaikishan P. Rajendrakumar M. S. Ramesha B. C. Viraktamath S. M. Balachandran C. N. Neeraja K. Sujatha K. Srinivasa Rao P. Natarajkumar Y. Hari K. Sakthivel A. S. Ramaprasad R. M. Sundaram 《Plant Breeding》2010,129(1):108-111
This study was undertaken to assess the comparative potential of 25 Expressed Sequence Tag derived simple sequence repeats (EST-SSRs) and 25 genomic SSRs in the prediction of grain yield heterosis using a set of nine cytoplasmic male sterile (CMS) lines and 32 restorer lines of rice. EST-SSRs and genomic SSRs exhibited an average Polymorphism Information Content value of 0.37 and 0.45, respectively. The coefficient of marker polymorphism among parental lines with respect to a set of hypervariable EST and genomic SSRs was correlated with standard heterosis for grain yield of six public bred rice hybrids. EST-SSRs gave a better correlation (r = 0.75) as compared with genomic SSRs (r = 0.09). When 10 'key' informative EST-SSR markers which showed a higher positive correlation with grain yield heterosis were validated in a new set of 14 experimental hybrids, the markers exhibited a higher correlation (r = 0.79), indicating the predictive value of these EST-SSRs. We recommend these 10 'key' informative EST-SSR markers for analysis of genetic diversity of parental lines and prediction of heterosis in hybrid rice breeding programmes. 相似文献