Die Mikronährstoffversorgung der Böden der Bezirke Halle und Magdeburg: 3. Mitteilung: Der Einfluß verschiedener Bodenfaktoren auf den Mn-Gehalt der Ackerböden     

Karlheinz Klemm 《Archives of Agronomy and Soil Science》2013,59(10):877-888

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In vitro characterization andin vivo detection ofRigidoporus lignosus,the causal agent of white root disease inHevea brasiliensis,by ELISA techniques     

M. Louanchi  P. Robin  T. Michels  M. H. Balesdent  D. Despréaux 《European journal of plant pathology / European Foundation for Plant Pathology》1996,102(1):33-44

The aim of these studies is to develop a method for early detection ofRigidopoms lignosus (Basidiomycete, Polyporaceae), the causative agent of white root disease of rubber tree. Two polyclonal sera were produced against soluble mycelial proteins of twoR. lignosus isolates, one from Africa (FCI2), the other from Asia (FID2). The specificity of the antisera was tested using isoelectric focusing (IEF)/Western-blot and DAS-ELISA. The two sera recognized all 20R. lignosus isolates from various geographical origins. The banding patterns obtained by Western-blot enabled a distinction to be made between isolates from Africa and those from Asia. In DAS-ELISA and Western-blot analyses, strong cross reactions were observed withR. ulmarius. Only slight reactions were observed in Western-blot analysis toR. lineatus andP. noxius, both causative agents of root rot inHevea. These cross reactions were not observed under our DAS-ELISA analysis conditions. Finally, no cross reactions were obtained with 9 otherPolyporaceae orHevea root pathogen species. The sensitivity threshold of the DAS-ELISA method was 5 ng ml^–1 ofR. lignosus protein. An initial approach to using the DAS-ELISA test for the detection ofR. lignosus in infected plants was carried out on artificially inoculated root samples. The DAS-ELISA protocol enabled detection ofR. lignosus in the root systems of diseased plants. Moreover, no cross reaction was observed with healthy plant extracts.  相似文献   

Prognosis following surgical excision of canine cutaneous mast cell tumors with histopathologically tumor-free versus nontumor-free margins: a retrospective study of 31 cases     

Michels GM  Knapp DW  DeNicola DB  Glickman N  Bonney P 《Journal of the American Animal Hospital Association》2002,38(5):458-466

The purpose of this study was to determine if the presence of histopathologically tumor-free versus nontumor-free margins was prognostic for relapse or tumor-related death in dogs following surgical excision of single or multiple cutaneous mast cell tumors confined to the skin without evidence of metastasis to lymph nodes or other noncutaneous sites. Differences in tumor-related death or frequency of relapse between the two groups were not significant. Failure to achieve histopathological tumor-free margins frequently did not lead to local relapse. All tumor-related deaths occurred following local relapse. The lack of statistical support for an association between prognosis and histopathological tumor-free versus nontumor-free margins may be a result of small sample size.  相似文献   

THE PHYSIOLOGIC RESPONSE OF DOMESTIC ANIMALS TO IONIZING RADIATION: A REVIEW     

Christina  von ZallingerDVM  Karlheinz  TempelDVM 《Veterinary radiology & ultrasound》1998,39(6):495-503

With respect to clinical signs of the radiation syndromes, some remarkable species variations exist. For example the marked delayed reaction of the acute hematologic response in cows. An unusually high sensitivity of the central nervous system is found in burros, which is probably caused by acute vascular and/or metabolic changes in the brain. The species-specific number of intestinal crypt and hemopoietic stem cells may explain the early survival differences among species after high doses of irradiation. Mortality due to acute radiation syndromes is lowest in chickens. Regarding late effects, various neoplasms are typical in dogs, and cattle more commonly develop cataracts.  相似文献   

OBITUARY     

Briggs LJ  Michels NA 《Science (New York, N.Y.)》1932,76(1963):137-138

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Trees and windbreaks in the Sahel: establishment,growth, nutritive,and calorific values     

J. P. A. Lamers  K. Michels  R. J. Vandenbeldt 《Agroforestry Systems》1994,26(3):171-184

Tree and windbreak species considered for the Southern Sahelian Zone (SSZ) of West Africa have to be evaluated following multiple criteria such as fast establishment, shelter efficiency, production of feed for livestock, and firewood. An on-station experiment was conducted on a sandy soil in southwest Niger between 1988 and 1993 to assess the establishment, growth, nutritive, and calorific values of seven species considered for windbreaks:Andropogon gayanus, Bauhinia rufescens, Acacia holosericea, Acacia nilotica, Acacia senegal, Faidherbia albida, andAzadirachta indica. Mortality and stem diameter were monitored twice a year. From 20 months after planting (MAP) onwards, species were annually trimmed to 2 m height and to 1 m sidewards of the main stem. Fresh and dry weight of leaves, twigs, wood, and fruits were recorded. Leaves were analyzed for crude protein (CP) and metabolizable energy (ME) content. Wood was analyzed for its gross calorific value and its ash content. At 56 MAP, all species had less than 5% morality, except forA. holosericea, which had a mortality rate of 15.9%.A. indica andA. holosericea showed the highest stem diameter among species with 12.2 and 11.8 cm, respectively, at 56 MAP.A. senegal had the highest dry matter production with 22.3 t ha^–1 at 56 MAP, followed byA. indica (12.0 t ha^–1),A. holosericea (11.7 t ha^–1) andB. rufescens (11.2 t ha^–1), butA. holosericea was most productive at earlier harvests. OnlyA. senegal (6.4 t ha^–1) andA. indica (5.1 t ha^–1) had a significant wood production, whereasA. holosericea (5.7 t ha^–1) had the highest phyllodes production. Leaves ofA. senegal andA. indica had highest CP contents of 258 and 214 g kg^–1, respectively. The leaves of all species exceptA. gayanus had a higher CP:ME ratio than natural pasture in the region. The calorific values of firewood did not differ significantly among the species.A. nilotica, the species with one of the lowest firewood production, had the highest Fuelwood Value Index of 6.6. The choice of species for planting trees and windbreaks in the SSZ must be oriented along these criteria. Further research should be directed towards cost-benefit analyses, land tenure and property rights in combination with surveys on local knowledge of rural people.  相似文献   

On-farm evaluation of ridging and residue management options in a Sahelian millet-cowpea intercrop. 2. Crop development     

C.L. Bielders  K. Michels 《Soil Use and Management》2002,18(4):309-315

Abstract. In the Sahel, promising technologies for agricultural intensification include millet stover mulching and ridging. A four year on‐farm experiment was set‐up in order to assess the effect of various combinations of these two technologies on crop development and yield in a millet (Pennisetum glaucum (L.) R. Br.) ‐ cowpea (Vigna unguiculata (L.) Walp.) intercropping system. Treatments included bare surface, ridging, a surface applied banded millet stover mulch (2 t ha^–1) and a banded millet stover mulch (2 t ha^–1) buried in ridges. The latter three treatments were implemented exclusively in the cowpea rows, with an annual rotation between the millet and cowpea rows. On bare and ridged plots, millet yields fell below 100 kg grain ha^–1 after the first year. This was ascribed mainly to soil acidification and loss of soil organic matter rather than to soil physical constraints or water availability despite extensive surface crusting and high soil penetration resistance and bulk density. Compared to the bare plots, ridging increased cowpea hay production by 330% over the four years which was attributed to lower soil penetration resistance and bulk density but also to a reduction of 0.15 cmol₊ kg^–1 exchangeable acidity in the ridges. Except during the severe drought year of 1997, millet grain yield in the banded mulch treatment remained fairly stable over time at 526 ± 9 kg ha^–1. However, a detailed analysis revealed yield compensation mechanisms between various yield components depending on the timing of occurrence of the abiotic stresses. Cowpea productivity was always higher in buried banded mulch plots than in surface applied banded mulch plots but the former treatment appeared unable to sustain millet yields. This decline was attributed to a greater nutrient uptake by cowpea and more rapid acidification in the buried mulch treatment compared to the banded mulch treatment.  相似文献   

Physical mapping of a translocation breakpoint in neurofibromatosis   总被引：13，自引：0，他引：13  

J W Fountain  M R Wallace  M A Bruce  B R Seizinger  A G Menon  J F Gusella  V V Michels  M A Schmidt  G W Dewald  F S Collins 《Science (New York, N.Y.)》1989,244(4908):1085-1087

The gene for von Recklinghausen neurofibromatosis (NF1), one of the most common autosomal-dominant disorders of humans, was recently mapped to chromosome 17 by linkage analysis. The identification of two NF1 patients with balanced translocations that involved chromosome 17q11.2 suggests that the disease can arise by gross rearrangement of the NF1 locus, and that the NF1 gene might be identified by cloning the region around these translocation breakpoints. To further define the region of these translocations, a series of chromosome 17 Not I-linking clones has been mapped to proximal 17q and studied by pulsed-field gel electrophoresis. One clone, 17L1 (D17S133), clearly identifies the breakpoint in an NF1 patient with a t(1;17) translocation. A 2.3-megabase pulsed-field map of this region was constructed and indicates that the NF1 breakpoint is only 10 to 240 kilobases away from 17L1. This finding prepares the way for the cloning of NF1.  相似文献   

Extraktion eines kupferbelasteten Bodens unter Verwendung eines aminosäurehaltigen Reststoffhydrolysats. 2. Elutionsdynamik und Bilanzierung der Aminosäuren nach kontinuierlichem Eintrag in Bodensäulen     

Klaus Fischer  Karlheinz Blay  Jarmila Kotalik  Peter Riemschneider  Dietmar Klotz  Antonius Kettrup 《植物养料与土壤学杂志》1997,160(4):511-518

Extraction of a copper contaminated soil material by the percolation of an amino acid containing residue hydrolysate. 2. Time course of amino acid elution and input/output balance of amino acids During 16 days an amino acid containing blood meal hydrolysate (amino acid concentration: 188 mMol·L^?1) was percolated through a column packed soil material (soil content per column: 4.1 kg dry weight, four parallels). The copper contaminated material (soil type: Typic Udifluvent, soil texture: sandy loam, loamy sand) was sampled from an area formerly used for cultivation of hop (Humulus lupus). Besides the investigation of the copper liberation the experiments aimed to determine the elution dynamic and input/output balance of amino acids (time span for amino acids balance 14 days). In total 11.7 L of hydrolysate, containing 2.2 Mol of amino acids, were introduced into each column. The mean amino acid output with the column effluent was 1.13 Mol. This corresponds to an elution degree of 51.2%, related to the sum of applicated amino acids, and to a mean substance specific elution degree of 48.4% reflecting the elution of 15 compounds. The substance specific elution ranged from 9.6% (serine) to 75.5% (valine). The highest concentrations of serine and threonine were determined in the effluents after two days, whereas the histidine concentration was highest at the last sampling. The differences in the percolation properties of the amino acids are discussed in terms of important retention and elimination processes (biodegradation, ad-/desorption, intercalation).  相似文献   

Anaemia, iron status and vitamin A deficiency among adolescent refugees in Kenya and Nepal     

Woodruff BA  Blanck HM  Slutsker L  Cookson ST  Larson MK  Duffield A  Bhatia R 《Public health nutrition》2006,9(1):26-34

OBJECTIVE: To investigate the prevalence of anaemia (haemoglobin<11.0 to 13.0 g dl(-1) depending on age and sex group), iron deficiency (transferrin receptor concentration>8.3 microg ml(-1)) and vitamin A deficiency (serum retinol <0.7 micromol l(-1)) in adolescent refugees. DESIGN: Cross-sectional surveys. SETTING: Kakuma refugee camp in Kenya and seven refugee camps in Nepal. SUBJECTS: Adolescent refugee residents in these camps. RESULTS: Anaemia was present in 46% (95% confidence interval (CI): 42-51) of adolescents in Kenya and in 24% (95% CI: 20-28) of adolescents in Nepal. The sensitivity of palmar pallor in detecting anaemia was 21%. In addition, 43% (95% CI: 36-50) and 53% (95% CI: 46-61) of adolescents in Kenya and Nepal, respectively, had iron deficiency. In both surveys, anaemia occurred more commonly among adolescents with iron deficiency. Vitamin A deficiency was found in 15% (95% CI: 10-20) of adolescents in Kenya and 30% (95% CI: 24-37) of adolescents in Nepal. Night blindness was not more common in adolescents with vitamin A deficiency than in those without vitamin A deficiency. In Kenya, one of the seven adolescents with Bitot's spots had vitamin A deficiency. CONCLUSIONS: Anaemia, iron deficiency and vitamin A deficiency are common among adolescents in refugee populations. Such adolescents need to increase intakes of these nutrients; however, the lack of routine access makes programmes targeting adolescents difficult. Adolescent refugees should be considered for assessment along with other at-risk groups in displaced populations.  相似文献