Operating a major electric utility today     

Nagel TJ 《Science (New York, N.Y.)》1978,201(4360):985-993

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Taenia solium oncosphere antigens induce immunity in pigs against experimental cysticercosis   总被引：11，自引：0，他引：11  

Verastegui M  Gilman RH  Gonzales A  Garcia HH  Gavidia C  Falcon N  Bernal T  Arana Y  Tsang VC;Cysticercosis Working Group In Peru 《Veterinary parasitology》2002,108(1):49-62

Immunity to Taenia solium infection was investigated using an experimental intramuscular oncosphere infection assay (IMOA) model in pigs. Three naturally infected pigs with cysticercosis were treated with oxfendazole (OFZ), a drug demonstrated to kill cysts in porcine muscle. These animals were then challenged with oncospheres but did not develop any cysts while three uninfected pigs that were similarly challenged, did develop intramuscular cysts. In another study, two groups of three pigs each were immunized with crude T. solium oncosphere and metacestode antigens, respectively, and tested with the IMOA. Immunization with crude oncosphere antigens (OAs) induced 100% protection, while metacestode antigens provided only partial protection. Immunoblots showed that pigs with complete immune protection to oncosphere intramuscular challenge had antibodies to two OAs at 31.3 and 22.5 kDa, respectively. Antibody to these two antigens was absent in pigs immunized with metacestodes or in uninfected control pigs. This study demonstrated the presence of two antigens that are unique to the oncosphere. Although, antibody to these two antigens is consistently present in pigs that are protected from an oncosphere intramuscular challenge their role in preventing infection by T. solium larval cysts is still hypothetical.  相似文献   

Effects of deficit irrigation strategies on cluster microclimate for improving fruit composition of Moscatel field-grown grapevines   总被引：1，自引：0，他引：1  

Tiago Pedreira dos Santos  Carlos M. Lopes  M. Lucília Rodrigues  Claudia R. de Souza  Jorge M. Ricardo-da-Silva  João P. Maroco  João S. Pereira  M. Manuela Chaves 《Scientia Horticulturae》2007

The grapevine plays a very important role in the economic, social and cultural sectors of many regions; however vineyards are often grown in regions under stressful conditions and thus they are vulnerable to climate change. The objective of this research was to investigate the effect of partial root-zone drying (PRD) irrigation on vine water relations, vegetative growth, plant microclimate, berry composition and yield components, compared to conventional deficit irrigation (DI, 50% ETc), full irrigation (FI, 100% of ETc) and non-irrigated vines (NI). The study was undertaken in mature ‘Moscatel’ grapevines (Vitis vinifera L.) grown in Pegões, South of Portugal. Compared to the other irrigated treatments, PRD vines showed a better microclimate at the cluster zone with higher incident photosynthetic photon flux density (PPFD). Within the more open canopies of NI and PRD treatments, berry temperatures were higher than those of denser ones (DI and FI). Compared to the conventional irrigation technique the better microclimate observed in PRD vines was a consequence of a reduction in vine growth, where lower values of leaf layer number, leaf area, canopy wideness, water shoots and shoot weight were observed. In PRD vines we observed a tendency to a development of a deeper root system, while DI and FI showed a more homogeneous root distribution throughout the different soil layers. PRD showed an improvement in berry quality with higher values of flavour precursors, and total phenols concentration without any significant yield reduction compared to DI and FI.  相似文献   

Avirulence and immunogenicity in mice of a bovine mastitis Staphylococcus aureus mutant.          下载免费PDF全文

C Bogni  M Segura  J Giraudo  A Giraudo  A Calzolari    R Nagel 《Canadian journal of veterinary research》1998,62(4):293-298

An avirulent mutant, designated RC122, was derived from Staphylococcus aureus bovine mastitis strain RC108 after N-methyl-N'-nitro-N-nitrosoguanidine mutagenesis. Mutant RC122, which was isolated on the basis of reduced colony size, showed diminished virulence in mice (LD50 of RC122: 3.1 x 10(10) cfu vs LD50 of RC108: 2.3 x 10(7) cfu). Mutant RC122 grew more slowly than its parental strain and showed decreased production of several exoproteins, such as alpha- and beta-hemolysin, DNAse and coagulase. The production of its capsule was induced only under in vivo growth conditions. Clearance studies performed in the mouse kidney revealed that the kinetics of disappearance of the mutant was similar to that of its parental strain. Protection experiments carried out by intraperitoneal administration in mice showed that mutant RC122 conferred a good degree of protection from challenge with homologous and heterologous strains.  相似文献   

Quantitative determination of duodenal juice in the cow     

R Krawielitzki  S Nagel  B Piatkowski 《Archives of Animal Nutrition》1974,24(4):309-313

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Case report of a special form of congenital femur defect, partial femoral aplasia in dogs     

M L Nagel  P Ost 《Tier?rztliche Praxis》1985,13(3):361-364

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Giardia duodenalis in colony stray cats from Italy     

Isabel Guadano Procesi  Azzurra Carnio  Federica Berrilli  Margherita Montalbano Di Filippo  Alessia Scarito  Cristina Amoruso  Marco Barni  Marco Ruffini  Giulia Barlozzari  Manuela Scarpulla  Claudio De Liberato 《Zoonoses and public health》2022,69(1):46-54

Giardia duodenalis is the most common intestinal protozoan in humans and animals worldwide, including eight morphologically identical assemblages, infecting pets, livestock, wildlife and human beings. Assemblages A and B are those with the higher zoonotic potential, and they have been detected in several mammals other than humans; the others (C to H) show a higher host specificity. Cats can harbour both the specific Assemblage F and the zoonotic ones A and B. Several studies have been carried out on G. duodenalis genotypes in cats; however, the role of this species in the epidemiology of giardiasis is still poorly understood. In this scenario, the present study carried out the detection and genetic characterization at sub-assemblage level of G. duodenalis from colony stray cats in central Italy. In the period 2018–2019, 133 cat faecal samples were analysed for the presence of G. duodenalis cysts by a direct immunofluorescence assay. Positive samples were subsequently subjected to molecular analyses for assemblage/sub-assemblage identification. Forty-seven samples (35.3%) were positive for G. duodenalis cysts by immunofluorescence. G. duodenalis DNA was amplified at SSU-rDNA locus from 39 isolates: 37 were positive for zoonotic Assemblage A and 2 showed a mixed infection (A + B). Positive results for the β-giardin gene were achieved for 25 isolates. Sequence analysis revealed 16 isolates belonging to Sub-assemblage AII and 8 to Sub-assemblage AIII. One isolate resulted as ambiguous AI/AIII. Large sequence variability at the sub-assemblage level was detected, with several double peaks and mutations, making complex a proper isolate allocation. When compared with previous studies, the 35.3% prevalence of G. duodenalis in cats reported in the present article was surprisingly high. Moreover, all positive cats resulted to be infected with zoonotic assemblages/sub-assemblages, thus indicating stray cats as a possible source of human giardiasis and highlighting the sanitary relevance of cat colonies in the study area.  相似文献   

A new dihydroxysterol from the marine phytoplankton Diacronema sp     

Rauter AP  Filipe MM  Prata C  Noronha JP  Sampayo MA  Justino J  Bermejo J 《Fitoterapia》2005,76(5):433-438

Diacronema sp. was cultured and its sterols were separated by column chromatography on silica gel. The new sterol 24-ethyl-4alpha-methyl-cholestane-3,20-diol (1) was characterised by NMR and MS spectrometry, as well as (22E)-24-ethyl-4alpha-methyl-5alpha-cholest-22-en-3beta-ol (2) and beta-sitosterol, the major components of the sterol fractions. Neither the biosynthetic origin of the new dihydroxysterol nor its role in the biochemistry of Diacronema is known.  相似文献   

A microwave tomographic system for wood characterization in the forest products industry     

Matteo Pastorino  Alessandro Fedeli  Andrea Salvadè  Samuel Poretti  Manuela Maffongelli 《Wood material science & engineering》2015,10(1):75-85

Abstract

Nondestructive testing and evaluation techniques able to extract information about the internal structure of the samples under test are very important in the wood industry. Microwave imaging systems have been considered for a long time promising apparatuses for this task. In this framework, approaches exploiting the full scattering phenomena for creating images of the distributions of the dielectric properties of the targets have been developed in the last few years. In this paper, a prototype of microwave tomographic system is presented and several experimental validation confirming its suitability for the use in the wood and forest product industry are reported.  相似文献   

Determination of cocoa butter equivalents in milk chocolate by triacylglycerol profiling     

Buchgraber M  Androni S  Anklam E 《Journal of agricultural and food chemistry》2007,55(9):3284-3291

An analytical approach for the detection and quantification of cocoa butter equivalents (CBEs) in milk chocolate is presented. It is based on (i) a comprehensive standardized database covering the triacylglycerol composition of a wide range of authentic milk fat (n=310), cocoa butter (n=75), and CBE (n=74) samples and 947 gravimetrically prepared mixtures thereof, (ii) the availability of a certified cocoa butter reference material (IRMM-801) for calibration, (iii) an evaluation algorithm, which allows a reliable quantification of the milk fat content in chocolate fats using a simple linear regression model, (iv) a subsequent correction of triacylglycerols deriving from milk fat, (v) mathematical expressions to detect the presence of CBEs in milk chocolate, and (vi) a multivariate statistical formula to quantify the amount of CBEs in milk chocolate. The detection limit was 1% CBE in chocolate fat (0.3% CBE in milk chocolate, having a fat content of 30%). For quantification, the average error for prediction was 1.2% CBE in chocolate fat, corresponding to 0.4% in milk chocolate (fat content, 30%).  相似文献