Comparative efficacy of three antiseptics as surgical skin preparations in dogs          下载免费PDF全文

Charles Boucher BVSc  MMedVet  Maryke M. Henton BVSc  MMedVet  Piet J. Becker BSc  MSc  PhD 《Veterinary surgery : VS》2018,47(6):792-801

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Genetic monitoring of ex situ African Penguin (Spheniscus demersus) populations in South Africa     

Christiaan Labuschagne  Lisa Nupen  Antoinette Kotzé  J Paul Grobler 《African Zoology》2016,51(2):83-90

The African Penguin (Spheniscus demersus) has suffered population declines and is listed in the IUCN Red List as Endangered. The species is endemic to the coast of southern Africa, and breeding colonies are distributed on the south-western coast of Africa. Currently, African Penguins are being kept in zoo and aquarium facilities throughout South Africa. In this study, molecular genetic data based on 12 microsatellite markers from 1 119 African Penguin samples from four facilities were generated in order to determine the level of genetic variation, population structure and differentiation, and effective population size to assist in the development of an effective captive management plan. Expected heterozygosity ranged from 0.57 to 0.62, and allelic richness from 4.2 to 5.1. However, based on differences between first- and second-generation captive birds, we conclude that the ex situ population is at risk of losing genetic variability in the future and management programmes should include exchange of birds between captive facilities in order to induce gene flow and increase effective population size. Adding individuals from in situ populations should also be considered in the future in cases where these birds cannot be rehabilitated. Molecular genetic analyses of wild penguin populations should be carried out for comparison, and to ascertain to what degree ‘in situ genetic diversity’ is represented among ex situ populations. With regular resampling and analyses, the extent of the effect of processes such as genetic drift on diversity in the ex situ penguin populations will become evident.  相似文献   

Culicoides biting midges at the National Zoological Gardens of South Africa     

Labuschagne K  Gerber LJ  Espie I  Carpenter S 《The Onderstepoort journal of veterinary research》2007,74(4):343-347

Culicoides biting midges (Diptera: Ceratopogonidae) are responsible for the transmission of a large number of pathogens to livestock and wild animals. In this study the presence of the genus, using light traps based at four different sites within the National Zoological Gardens of South Africa, was investigated during 2002-2004. In total, 37 species were recorded, including large numbers of Culicoides imicola Kieffer, 1913, which is responsible for the transmission of economically important arboviruses in South Africa, Europe, Middle and Far East. These results are discussed with reference to the wider Culicoides fauna in the Onderstepoort area of South Africa, their vector competence as well as biosecurity at the National Zoological Gardens.  相似文献   

Effect of pavetamine on protein synthesis in rat tissue     

Schultz RA  Fourie N  Basson KM  Labuschagne L  Prozesky L 《The Onderstepoort journal of veterinary research》2001,68(4):325-330

Pavetamine, the active principle of plants causing gousiekte in ruminants, was found in this study to be an inhibitor of protein synthesis in the rat heart. Sprague-Dawley rats were injected intra-peritoneally with 8-10 mg/kg pavetamine and the levels of protein synthesis in different organs determined utilizing L-[4-3H]phenylalanine incorporation. In contrast to the more than 23% inhibition found in heart tissue at 4, 24 and 48 h after administration of pavetamine, the effect on the kidney, liver, spleen, intestine and skeletal muscle was minimal or returned to pretreatment levels within 48 h. These results may offer an explanation for the clinical signs observed in ruminants with gousiekte, where the heart only is affected.  相似文献   

Neurotoxicity in calves induced by the plant, Nierembergia hippomanica Miers var. violacea Millán in South Africa     

Botha CJ  Schultz RA  Van Der Lugt JJ  Retief E  Labuschagne L 《The Onderstepoort journal of veterinary research》1999,66(3):237-244

The plant Nierembergia hippomanica var. violacea has been incriminated in field outbreaks of neurotoxicity in calves in the Free State Province. Hepatotoxicity and electrocardiogram (ECG) deviations were induced in a sheep dosed with 5 g/kg dried plant material on four consecutive days. A calf dosed with 2.5 g/kg dried plant material, on two consecutive days, did not show overt clinical changes. Voluntary ingestion of approximately 30 g/kg fresh flowering plants by a second calf resulted in nervous signs characterized by chewing motions, protrusion of the tongue, dysphagia, hypermetria, ataxia, paresis and lateral recumbency. Salivation, dehydration and cardiac irregularities completed the clinical picture. Clinical chemistry changes revealed muscle damage and increased serum urea and creatinine concentrations indicative of kidney involvement. This is the first confirmed outbreak of Nierembergia hippomanica var. violacea intoxication of stock in South Africa.  相似文献   

The potential of nonpathogenic Fusarium oxysporum and other biological control organisms for suppressing fusarium wilt of banana   总被引：3，自引：0，他引：3  

B. Nel  C. Steinberg  N. Labuschagne  A. Viljoen 《Plant pathology》2006,55(2):217-223

The aim of this study was to evaluate the ability of nonpathogenic F. oxysporum and Trichoderma isolates from suppressive soils in South Africa to suppress fusarium wilt of banana in the glasshouse. Several biological control agents and commercial biological control products were included in the study. The isolates were first screened in vitro on potato dextrose agar. In glasshouse evaluations, the fungal and bacterial isolates were established on banana roots before they were replanted in pathogen-infested soil, while the commercial biocontrol agents were applied as directed by the supplier. Banana plantlets were evaluated for disease development after 7 weeks. In vitro tests showed none of the nonpathogenic isolates suppressed Fusarium oxysporum f.sp. cubense ( Foc ), while slight suppression was observed with the two Trichoderma isolates. Results of the glasshouse evaluations revealed that two of the nonpathogenic F. oxysporum isolates, CAV 255 and CAV 241, reduced fusarium wilt incidence by 87·4 and 75·0%, respectively. The known biological control agent Fo47 did not suppress Foc significantly. Pseudomonas fluorescens strain WCS 417, known for its ability to suppress other fusarium wilt diseases (WCS 417), reduced disease incidence by 87·4%. These isolates should be further evaluated for potential application in the field, independently and in combination.  相似文献   

General combining ability of six genotypes of spring wheat (Triticum aestivum) for biscuit-making quality characteristics     

M. T. Labuschagne    M. C. B. Coetzee  C. S. van  Deventer 《Plant Breeding》1996,115(4):279-281

The first South African soft wheat breeding programme was initiated in 19 90 , consequently almost no information is available on soft wheat quality characteristics. The aim of this study was to determine general combining ability (GCA) of several spring wheats for biscuit-making quality characteristics. A full dialled cross was made with five spring wheats with good biscuit-making quality and a sixth hard wheat with poor biscuit-making quality. Seventeen quality characteristics were measured. When the GCA to specific combining ability (SCA) ratios were calculated, strong additive gene action was shown for all characteristics, excluding flour colour and biscuit diameter, which suggested high heritabilities for most characteristics. None of the characteristics showed more non-additive than additive gene action. Three of the soft wheat cultivars combined well for the characteristics important for biscuit-making quality.  相似文献   

Chromosome locations of leaf rust resistance genes in selected tetraploid wheats through substitution lines     

Shimelis?HusseinEmail author  Johan?J.?Spies  Zakkie?A.?Pretorius  Maryke?T.?Labuschagne 《Euphytica》2005,141(3):209-216

Langdon durum D-genome disomic substitution lines were used to study the chromosome locations of adult-plant leaf rust resistance genes identified from tetraploid wheat accessions. The accessions are 104 (Triticum turgidum subsp. dicoccum var. arras) and 127 (T. turgidum subsp. durum var. aestivum). The complete sets of the substitution lines were crossed as female parents with the accessions and F₁ double monosomic individuals selected at metaphase I. Segregating F₂ individuals were inoculated during the flag leaf stage with pathotype UVPrt2 of Puccinia triticina. The substitution analysis involving accession 104 showed that the gene for leaf rust resistance is located on chromosome 6B. The analysis with accession 127 indicated that chromosome 4A carries a gene for leaf rust resistance. The two novel genes are temporarily designated as Lrac104 and Lrac127, respectively from accessions 104 and 127.  相似文献   

Levels of host plant resistance to banana weevil, Cosmopolites sordidus (Germar) (Coleoptera: Curculionidae), in African Musa germplasm     

Andrew Kiggundu  Clifford S. Gold  Maryke T. Labuschagne  Dirk Vuylsteke  Schalk Louw 《Euphytica》2003,133(3):267-277

Forty-five Musa clones, including endemic and introduced cultivars plus hybrids, were evaluated for resistance against the banana weevil, Cosmopolites sordidus, in a field trial in Uganda. The predominant groups of staple crops, East African highland bananas (Musa spp. AAA) and plantains (Musaspp. AAB), as well as plantain-derived hybrids (AAB × AA), showed the highest levels of susceptibility to this pest. These were followed by dessert bananas (Musa spp. AAA), exotic bananas (Musa spp. ABB) and finally diploids of M. acuminata (AA). Hybrids of banana origin were highly resistant. Some East African highland cultivars, especially brewing types (e.g., Kabula, Bagandeseza, Ediirira), showed intermediate levels of resistance. Among the non-highland bananas, high levels of resistance were observed in Yangambi-Km5 (AAA), Cavendish (AAA), Gros Michel (AAA), Kayinja (ABB, Pisang Awak subgroup), Ndiizi (AB, Ney Poovan subgroup)and Kisubi (Ney Poovan subgroup). The highest resistance was observed in banana hybrids TMB2×7197-2, TMB2×8075-7 and the wild banana Calcutta-4 (AA). These were considered the best sources of resistance for a weevil resistance-breeding programme with the two hybrids commonly used as improved male parents. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   

Diversity in starch, protein and mineral composition of sorghum landrace accessions from Ethiopia   总被引：1，自引：1，他引：0  

Abe Shegro  Nemera Geleta Shargie  Angeline van Biljon  Maryke Tine Labuschagne 《Journal of Crop Science and Biotechnology》2012,15(4):275-280

Sorghum (Sorghum bicolor (L.) Moench) is an important food crop in arid and semi-arid tropics where it is grown mainly due to its drought tolerance. In Ethiopia, sorghum is grown in different agro-ecological zones primarily for food. Landraces are widely cultivated in the region due to their adaptation to the growing conditions and other characteristics such as food quality, quantity, and quality of by-products. The objective of this research was to determine the genetic variation in protein, mineral composition, total starch and its components in sorghum landraces collected from the western regions of Ethiopia. Sorghum whole grains were analyzed for crude protein, total starch and its components, and mineral profile (calcium, magnesium, potassium, phosphorus, iron, manganese, zinc, and sodium). The analysis of variance showed significant differences among the sorghum landraces in nutritional composition. There was highly significant correlation between phosphorus and magnesium, and between zinc and phosphorus and protein which indicated that some interaction existed in absorption and translocation between minerals and protein content. The genetic variation found among the sorghum landrace accessions in chemical composition can be exploited in the breeding program.  相似文献