Cell lines and plants obtained after protoplast fusions of Rosa+Rosa,Rosa+Prunus and Rosa+Rubus     

Squirrell  J.  Mandegaran  Z.  Yokoya  K.  Roberts  A. V.  Mottley  J. 《Euphytica》2005,146(3):223-231

Summary Protoplasts of three Rosa cultivars were fused with each other, with protoplasts of Prunus `Colt' and with protoplasts of Rubus laciniatus, using polyethylene glycol 4000 as a fusogen. Protoplasts of Prunus were incapable of cell division and those of Rosa and Rubus were disabled by treatments with metabolic inhibitors, either iodoacetate (IOA) or rhodamine 6-G (R6G). Parental protoplasts were then fused in combinations that required complementation for their survival. RAPD analysis of 41 fusion-derived cell lines showed that two lines resulting from fusions of Rosa + Rosa and one from a fusion of Rosa + Prunus, contained some DNA markers from both fusion partners. The others contained markers of only one fusion parent. This showed that after protoplast fusion, the heterokaryons did not develop into cell lines with stable hybrid nuclei. Plants regenerated from cell lines derived from Rosa + Prunus and Rosa + Rubus fusions contained DNA markers of only Rosa and their DNA amounts were no greater than that of the Rosa parent. However, they differed morphologically from the Rosa parent to a remarkable degree, possibly because they inherited undetected genes of Prunus or Rubus, or because they were somaclonal variants of the Rosa parent. Alternative strategies for the production of somatic hybrids are discussed.  相似文献   

Studies on the modes of action of n-alkylguanidines and triorganotins on photosynthetic energy conservation in the pea and the unicellular alga Chlamydomonas reinhardi Dangeard     

J. Mottley 《Pesticide biochemistry and physiology》1978,9(3):340-350

The pea (Pisum sativum L.) and the CW 15 (cell wall-less) strain of the unicellular alga Chlamydomonas reinhardi Dangeard were used to investigate the modes of action of two n-alkylguanidines and several triorganotin salts on reactions associated with photosynthetic energy conservation. The two n-alkylguanidines, n-octylguanidine and n-dodecylguanidine, affected state 3 (ADP stimulated) noncyclic electron transport of pea chloroplasts in a concentration-dependent manner, viz, energy transfer inhibition at low concentrations, uncoupling of electron transport from photophosphorylation at intermediate concentrations, and direct inhibition of electron transport at relatively high concentrations. The Ca²⁺-dependent ATPase (EC 3.6.1.5) activity of C. reinhardi chloroplast fragments was inhibited by both n-alkylguanidines over concentration ranges similar to those required to affect state 3 electron transport in pea chloroplasts. Dodecylguanidine required lower concentrations than octylguanidine to produce similar effects in both assays. The triorganotins produced uncoupler-reversible inhibition of the state 3 electron transport of pea chloroplasts in a sucrose medium and uncoupled the electron transport in a medium containing high chloride concentrations, as shown by their stimulation of both the light-induced transmission changes and the decay of the light-induced pH gradients of chloroplast suspensions. The inhibition of the Ca²⁺-dependent ATPase activity of C. reinhardi by most of the triorganotins was correlated to their inhibition of the growth of the alga on agar medium under different trophic conditions. The efficacies of the tri-n-alkyltins in all the assays used generally increased along the homologous series.  相似文献   

Experimental Hendra virus infection of dogs: virus replication,shedding and potential for transmission          下载免费PDF全文

DJ Middleton  S Riddell  R Klein  R Arkinstall  J Haining  L Frazer  C Mottley  R Evans  D Johnson  J Pallister 《Australian veterinary journal》2017,95(1-2):10-18

  相似文献   

THE EFFECT OF TEMPERATURE ON THE NUMBER OF SCALES IN TROUT     

Mottley CM 《Science (New York, N.Y.)》1931,74(1917):316

  相似文献