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1.
The effects of azadirachtin (AZA) and nucleopolyhedrovirus (NPV) on midgut enzyme activity in Spodoptera litura Fabricius (Lepidoptera: Noctuidae) (Tobacco cutworm) were evaluated. Gut enzyme activities were decreased by AZA and NPV individually and in combination. When S. litura larvae were fed a diet of castor leaves treated with AZA and NPV in bioassays, gut enzyme—acid phosphatases, alkaline phosphatases, adenosine triphosphatases, and lactate dehydrogenase—activities were decreased. There were statistically significant differences (P ? 0.05) in enzyme activities between combined and individual treatment. A synergistic effect of botanical insecticides and virus was found when combined in low doses. These effects are most pronounced in early instars. Maximum weight loss (59-72%) occurred, when AZA and NPV were combined. 相似文献
2.
Geographical isolates ofSpodoptera litura (Fabricius) (Noctuidae: Lepidoptera) nucleopolyhedrovirus (SpltNPV), collected from different parts of India and maintained
at Tamil Nadu Agricultural University, were compared for their biological activity and subjected to Restriction Endonuclease
(REN) analysis. Neonate and second instar bioassay studies revealed similarity in biological activity as shown by the overlapping
fiducial limits of LC50 values. However, there were differences in yield among isolates: significantly higher yields were obtained from isolates
UAS and CBE than from the BARC isolate. REN analysis of the four isolates withPst I,Hind III,Bam HI andEco RI enzymes indicated genotypic variation among the isolates. Based on the commonality of the bands, the isolates could be
broadly divided into two groups: isolates AU and CBE formed one group, and the other group comprised UAS and BARC based on
genetic relatedness.
http://www.phytoparasitica.org posting Nov. 21, 2005. 相似文献
3.
Fatty acids are the largest component of lipids and have become a useful tool in the determination of live feeds to a variety of cultured species. Bioencapsulation is a technique which allows high-level incorporation of desired components (i.e., fatty acids, vitamins, antibiotics, etc.) in live feeds, which in turn can be supplemented to the consumer organisms. The procedure described in the present study serves as a platform of technology for enriching the Streptocephalus dichotomus. Uptake of two enrichment diets (ALGAMAC2000 and DHA-SELCO) by adult S. dichotomus was investigated. The fatty acid profile supports the hypothesis that the enrichment diet increases the level of essential fatty acids, such as linolic, linolenic, eicosapentenoic, and docosahexaenoic acids. The average content (percent of total fatty acids detected) of the enriched organism by different highly unsaturated fatty acid (HUFA) products were as follows: ALGAMAC2000 showed 14-22% saturated fatty acid (SFA), 17-18% monounsaturated fatty acid (MUFA), 28-41% polyunsaturated fatty acid (PUFA), 23-34% n-3, and 4.9-7.5% n-6, whereas DHA-SELCO showed about 20-23% SFA, 20-26% MUFA, 38% PUFA, 28-31% n-3, and 7.5-10% n-6. Our present investigation proves that both HUFA-rich diets appear to be an appropriate enrichment diet, and further provides an additional rationale for using fairy shrimp as a maturation diet for any cultivable freshwater organism. 相似文献
4.
Senthil T Vishwanath A Balents L Sachdev S Fisher MP 《Science (New York, N.Y.)》2004,303(5663):1490-1494
The theory of second-order phase transitions is one of the foundations of modern statistical mechanics and condensed-matter theory. A central concept is the observable order parameter, whose nonzero average value characterizes one or more phases. At large distances and long times, fluctuations of the order parameter(s) are described by a continuum field theory, and these dominate the physics near such phase transitions. We show that near second-order quantum phase transitions, subtle quantum interference effects can invalidate this paradigm, and we present a theory of quantum critical points in a variety of experimentally relevant two-dimensional antiferromagnets. The critical points separate phases characterized by conventional "confining" order parameters. Nevertheless, the critical theory contains an emergent gauge field and "deconfined" degrees of freedom associated with fractionalization of the order parameters. We propose that this paradigm for quantum criticality may be the key to resolving a number of experimental puzzles in correlated electron systems and offer a new perspective on the properties of complex materials. 相似文献
5.
Entomophaga aulicae (Hoffman in Bail) Batko (Zygomycotina: Entomophthorales) was found to cause epizootics in Spilarctia obliqua (Walker) (Arctiidae: Lepidoptera), a polyphagous pest in Manipur, India. Infected caterpillars climbed to elevated positions
on plants where they hung upside down, attached by their abdominal prolegs. Infection levels reached up to 57%. The conidiophores
are simple, conidia are ovoid to pyriform in shape, and the nuclei stained readily in aceto-orcein. Secondary, tertiary conidia
and resting spores were also observed. No rhizoids and cystidia were found. This is the first report of E. aulicae infecting S. obliqua in Manipur. 相似文献
6.
Recombinant LipL32 antigen-based single serum dilution ELISA for detection of canine leptospirosis 总被引：4，自引：0，他引：4
Dey S Mohan CM Kumar TM Ramadass P Nainar AM Nachimuthu K 《Veterinary microbiology》2004,103(1-2):99-106
A recombinant antigen-based single serum dilution enzyme-linked immunosorbent assay (ELISA) was developed to measure the specific antibody activity in sera of dogs with leptospirosis. The recombinant antigen developed and used in the assay was specific for the pathogenic serovars of Leptospira. A linear relationship was found to exist between the predicted antibody titres at a single working dilution of 1:1000 and the corresponding observed serum titres as determined by the standard serial-dilution method. Regression analysis was used to determine a standard curve from which an equation can be derived that allows demonstration of the mentioned correlation. The equation was then used to convert the corrected absorbance readings of the single working dilution directly into the predicted ELISA antibody titres. The assay was proved to be sensitive, specific and accurate as compared to the standard microscopic agglutination test (MAT). 相似文献
7.
Vishnu Sukumari Nath Muthukrishnan Senthil Vinayaka Mahabaleswar Hegde Muthulekshmi Lajapathy Jeeva Raj Shekhar Misra Syamala Swayamvaran Veena Mithun Raj 《European journal of plant pathology / European Foundation for Plant Pathology》2013,136(3):483-494
The oomycete Phytophthora colocasiae that causes taro leaf blight is the most devastating disease of taro and is widely distributed worldwide. Molecular and phenotypic techniques were employed for assessing and exploiting the genetic variability among four populations of P. colocasiae obtained from a fine spatial scale (multiple leaf blight lesions on single taro leaf). Phenotypic characters such as virulence, morphology and mating type showed no variation. ITS characterization revealed detectable polymorphism among isolates of P. colocasiae. The mean number of haplotypes (H), haplotype diversity (HD), nucleotide diversity (π), and nucleotide substitution rate (θ) among analyzed sequences were 6.75, 1.00, 0.069, and 0.088 respectively. High levels of inter and intra specific variation were detected by random amplified polymorphic DNA (RAPD) assays. Moderate genetic diversity (H?=?0.2651) was observed among populations of P. colocasiae. Analysis of molecular variance (AMOVA) confirmed that most of the genetic variability was confined to within a population (63.54 %). The coefficient of genetic differentiation among populations (G ST ) was 0.2007 and estimates of gene flow (Nm) among populations was 1.991 migrants per generation. Cluster analysis using UPGMA revealed that individuals from the same population failed to cluster in one distinct group. The results of the study reveal considerable genetic diversity among and within populations of P. colocasiae obtained from fine spatial scale. The possible mechanisms and implications of this genetic variation are discussed. 相似文献
8.
Kurunthachalam Senthil Kumar Kenneth S. Sajwan Ashok K. Alva Sellamuthu Manian 《Archives of Agronomy and Soil Science》2013,59(2):205-219
Abstract Litter decomposition rate, changes in macronutrients such as nitrogen (N), phosphorus (P) and potassium (K) from different grades of litter decomposition and occurrence of soil microfungi were investigated in a Cymbopogon polyneuros-dominated tall grass ecosystem from a burned and an unburned site in southern India. The litter decomposition rate was higher at the burned site than at the unburned site. This rate was also higher when the litter was mixed with the mineral soil material than leaving the litter unaffected on the soil surface. The concentrations of N, P, and K in the litter decreased as a result of progressed litter decomposition. Occurrence of microfungi identified from the different decomposition grades of the Cymbopogon polyneuros litter was higher at the burned site compared to the unburned site. Microfungal species present at both sites showed only minor differences. 相似文献
9.
Secondary metabolites from Dysoxylum malabaricum and Dysoxylum beddomei were tested against mature and immature stage of the mosquito vector Anopheles stephensi under laboratory conditions. The triterpenes 3beta,24,25-trihydroxycycloartane and beddomeilactone from D. malabaricum and D. beddomei showed strong larvicidal, pupicidal and adulticidal activity. They also affected the reproductive potential of adults by acting as oviposition deterrents. The highest concentration tested (10 ppm) of both compounds evoked more than 90% mortality and oviposition deterrence. 相似文献
10.
Two field experiments were carried out one after the other to study the effect of date of sowing and soil temperature on the soil nitrogen availability and yield of sorghum (Co. 25). Crops sown at weekly intervals were the treatments. Mean soil temperature at different depths, available soil N at different stages, leaf area index were assessed and correlation among different parameters established. The results indicated that time of sowing sorghum crop influenced the soil available N due to variations in soil temperature and the eanopv development at different levels as measured by leaf area indices.
Mean soil temperature increased with depths upto 30 cm. Also available N varied at different stages of crop growth but invariably higher in early sown crops Qanuary sown). Soil temperature at 15 cm depth influenced the available soil N most and consequently the leaf area index. This was reflected in the yield also. 相似文献
Mean soil temperature increased with depths upto 30 cm. Also available N varied at different stages of crop growth but invariably higher in early sown crops Qanuary sown). Soil temperature at 15 cm depth influenced the available soil N most and consequently the leaf area index. This was reflected in the yield also. 相似文献