排序方式： 共有21条查询结果，搜索用时 15 毫秒
1.
He H Liyanarachchi S Akagi K Nagy R Li J Dietrich RC Li W Sebastian N Wen B Xin B Singh J Yan P Alder H Haan E Wieczorek D Albrecht B Puffenberger E Wang H Westman JA Padgett RA Symer DE de la Chapelle A 《Science (New York, N.Y.)》2011,332(6026):238-240
Small nuclear RNAs (snRNAs) are essential factors in messenger RNA splicing. By means of homozygosity mapping and deep sequencing, we show that a gene encoding U4atac snRNA, a component of the minor U12-dependent spliceosome, is mutated in individuals with microcephalic osteodysplastic primordial dwarfism type I (MOPD I), a severe developmental disorder characterized by extreme intrauterine growth retardation and multiple organ abnormalities. Functional assays showed that mutations (30G>A, 51G>A, 55G>A, and 111G>A) associated with MOPD I cause defective U12-dependent splicing. Endogenous U12-dependent but not U2-dependent introns were found to be poorly spliced in MOPD I patient fibroblast cells. The introduction of wild-type U4atac snRNA into MOPD I cells enhanced U12-dependent splicing. These results illustrate the critical role of minor intron splicing in human development. 相似文献
2.
Jain M Nilsson R Sharma S Madhusudhan N Kitami T Souza AL Kafri R Kirschner MW Clish CB Mootha VK 《Science (New York, N.Y.)》2012,336(6084):1040-1044
Metabolic reprogramming has been proposed to be a hallmark of cancer, yet a systematic characterization of the metabolic pathways active in transformed cells is currently lacking. Using mass spectrometry, we measured the consumption and release (CORE) profiles of 219 metabolites from media across the NCI-60 cancer cell lines, and integrated these data with a preexisting atlas of gene expression. This analysis identified glycine consumption and expression of the mitochondrial glycine biosynthetic pathway as strongly correlated with rates of proliferation across cancer cells. Antagonizing glycine uptake and its mitochondrial biosynthesis preferentially impaired rapidly proliferating cells. Moreover, higher expression of this pathway was associated with greater mortality in breast cancer patients. Increased reliance on glycine may represent a metabolic vulnerability for selectively targeting rapid cancer cell proliferation. 相似文献
3.
Chettry Upasna Chrungoo Nikhil K. 《Plant foods for human nutrition (Dordrecht, Netherlands)》2021,76(4):399-409
Plant Foods for Human Nutrition - While intensification of farming systems is essential for achieving the Millennium Development Goal of “Zero hunger”, issues such as availability of... 相似文献
4.
Anil K Puniya Abdelfattah Z M Salem Sanjay Kumar Sumit S Dagar Gareth W Griffith Monica Puniya Sreenivas R Ravella Nikhil Kumar Tejpal Dhewa Ravinder Kumar 《农业科学学报》2015,14(3):550-560
To keep the concept of a safe food supply to the consumers, animal feed industries world over are showing an increasing interest in the direct-fed microbials(DFM) for improved animal performance in terms of growth or productivity. This becomes all the more essential in a situation, where a number of the residues of antibiotics and/or other growth stimulants reach in milk and meat with a number of associated potential risks for the consumers. Hence, in the absence of growth stimulants, a positive manipulation of the rumen microbial ecosystem to enhance the feedstuff utilization for improved production efficiency by ruminants has become of much interest to the researchers and entrepreneurs. A few genera of live microbes(i.e., bacteria, fungi and yeasts in different types of formulations from paste to powder) are infrequently used as DFM for the domestic ruminants. These DFM products are live microbial feed supplements containing naturally occurring microbes in the rumen. Among different DFM possibilities, anaerobic rumen fungi(ARF) based additives have been found to improve ruminant productivity consistently during feeding trials. Administration of ARF during the few trials conducted, led to the increased weight gain, milk production, and total tract digestibility of feed components in ruminants. Anaerobic fungi in the rumen display very strong cell-wall degrading cellulolytic and xylanolytic activities through rhizoid development, resulting in the physical disruption of feed structure paving the way for bacterial action. Significant improvements in the fiber digestibility were found to coincide with increases in ARF in the rumen indicating their role. Most of the researches based on DFM have indicated a positive response in nutrient digestion and methane reducing potential during in vivo and/or in vitro supplementation of ARF as DFM. Therefore, DFM especially ARF will gain popularity but it is necessary that all the strains are thoroughly studied for their beneficial properties to have a confirmed ‘generally regarded as safe' status for ruminants. 相似文献
5.
A series of hot brand monoazo reactive dyes (9a-l) were obtained by the coupling of diazotized 1Hbenzo[ g]pyrazolo[3,4-b]quinoline-3-ylamine (5) with various cyanurated coupling components (8a-l) in good yield. Synthesized dyes were characterized by various spectroscopic techniques. Their dyeing performances as reactive dyes have been assessed on silk, wool, and cotton fabrics. The percentage dye bath exhaustion and fixation on different fibers were found to be very good. The dyed fabric showed moderate to very good light fastness and good to excellent washing and rubbing fastness properties. Spectral properties and colorimetric data of synthesized dyes have also been studied in detail. 相似文献
6.
Heterocyclic monoazo quinazolinone based azo dyes derived by the diazotization of 3-(4-aminophenyl)-2-phenylquinazolin-4(3H)-one
with various phenyl pyrazolones based coupling components. All the heterocyclic azo dyes have been characterized by their
percentage yield, UV-VIS spectroscopy, elemental analysis, IR spectroscopy, 1H NMR spectroscopy, and dyeing performance on silk, wool, nylon, and polyester fibers. All the dyes gave moderate to excellent
fastness properties on each fiber. The main focus was to synthesize heterocyclic monoazo dyes that give good dyeing property
along with pharmacological activity (anti bacterial and antifungal). Therefore, the synthesized compounds were examined for
their antimicrobial activity at various concentrations using well-known Kirby-Bauer disk diffusion method. 相似文献
7.
SDS PAGE of endosperm proteins and␣RAPD profiles from different accessions of Himalayan buckwheat were studied to determine
their genetic variation and phylogenetic relationships. Comparisons based on Jaccard’s coefficient and UPGMA clustering revealed
interrelationships broadly in conformity with conventional treatments. Cluster analysis of the endosperm protein profiles
of the selected accessions revealed three broad clusters. A moderate level of intraspecific variability was detected in the
endosperm protein profile of different accessions of Fagopyrum esculentum, Moench. Three subgroups were detected in cluster 1. Subgroup 1 included varieties designated as Local, Kamroo local, OC-2
and␣VL-7 which were collected from VPKAS, Almora. Local, Kamroo local and OC-2 showed a similarity coefficient of 1.0 inspite
of their being identified as different accessions. VL-7 emerged out separately from the rest of the three accessions. Accessions
having winged grains and those having striations on the seed coat formed a 2nd and 3rd subgroup, respectively. IC-13145 which
been identified as “F. himalianum”, showed 100%␣similarity in endosperm protein profile with IC-13376 (F. esculentum) and 85–90% similarity which other accessions of F. esculentum. Our results indicate that “F. himalianum” belongs to the esculentum group and should not be regarded as a different species. Cluster 2 included all the accessions of F. tataricum (L.) Gaertn. with Sangla 1, 2, 3, 5, 6, 7 and IC-412863 showing 100% similarity. F. cymosum emerged as a separate group distinct from both esculentum and tataricum. Accessions of F. tataricum and F. cymosum did not show significant intraspecific variation in the SDS PAGE profile of endosperm proteins. Out of the 20 primers used,
3 generated robust, easily interpretable amplified products. While a 1490 bp and a 300 bp RAPD was detected only in F. tataricum, a 1154 bp RAPD was detected in all accessions of F. tataricum except in Shimla B-1. This variety is early maturing and has high seed yields. 相似文献
8.
Singh Mohar Malhotra Nikhil Singh Kuldeep 《Genetic Resources and Crop Evolution》2021,68(6):2181-2205
Genetic Resources and Crop Evolution - The narrow genetic base of cultivated chickpea (Cicer arietinum L.) has hindered the progress in realizing high genetic gains in chickpea breeding programs.... 相似文献
9.
Anil K Puniya Abdelfattah Z M Salem Sanjay Kumar Sumit S Dagar Gareth W Grififth Monica Puniya Sreenivas R Ravella Nikhil Kumar Tejpal Dhewa Ravinder Kumar 《中国农业科学(英文版)》2015,(3)
To keep the concept of a safe food supply to the consumers, animal feed industries world over are showing an increasing interest in the direct-fed microbials (DFM) for improved animal performance in te... 相似文献
10.
Saikat P. Biswas Arun G. Jadhao Nikhil V. Palande 《Fish physiology and biochemistry》2014,40(2):457-467
We are reporting for the first time that the catecholamines (adrenaline and noradrenaline) inhibit the effect of nitric oxide (NO) on melanosome dispersion in freshly isolated scales of the freshwater snakehead fish, Channa punctatus. We studied the effect of NO and catecholamines on the pigment displacement by observing the changes in the melanophore index. The scales when treated with solution containing NO donor sodium nitroprusside (SNP) showed dispersion of melanosomes, whereas NO synthase blocker N-omega-Nitro-l-arginine suppresses this action of SNP. Treatment with adrenaline and noradrenaline on the isolated scales caused aggregation of melanosomes. Scales treated with solution containing catecholamines and SNP resulted in aggregation of melanosomes suggesting that catecholamines mask the effect of SNP. These results suggest that the catecholamines are inhibiting the effect of NO and causing the aggregation of the melanosomes may be via surface receptors. 相似文献