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1.
Imports of pesticides have long been subsidised for use in the cocoa agroforests of the humid forest zone of southern Cameroon. With the liberalisation of the cocoa and pesticides sectors and the devaluation of the local currency (CFA franc), farmers are facing fluctuations in the price paid for cocoa and the high cost of farm inputs. Without the support of the extension services, they themselves have developed traditional integrated control methods based on the use of plant extracts mixed with conventional pesticides. From a survey of 300 cocoa farmers, the study assesses the farmers' command of these methods, the pests controlled, the problems encountered and the institutional constraints in the definition and dissemination of integrated control methods. The study concludes with recommendations for research and development towards the better definition and dissemination of integrated control methods. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   
2.
The extent of genetic diversity and relatedness of cowpea germplasm from East Africa are poorly understood. A set of 13 microsatellites (SSR) and 151 single nucleotide polymorphisms (SNPs) markers were applied to assess the levels of genetic diversity in a sample of 95 accessions of local cowpea germplasm and inbred lines of Vigna unguiculata. The average genetic diversity (D), as quantified by the expected heterozygosity, was higher for SSR loci (0.52) than for SNPs (0.34). The polymorphic information content was 0.48 for SSR and 0.28 for SNP while the fixation index was 0.095 for SSR and 0.15 for SNPs showing moderate differentiation and high gene flow among cowpea accessions from East African countries. The results of data analysis of both SSR and SNP markers showed similar clustering patterns suggesting a substantial degree of association between origin and genotype. Principal coordinate analysis (PCoA) with SSR and SNP markers showed that accessions were grouped into two and three broad groups across the first two axes, respectively. Our study found that SNP markers were more effective than SSR in determining the genetic relationship among East African local cowpea accessions and IITA inbred lines. Based on this analysis, five local cowpea accessions Tvu-13490, Tvu-6378, Tvu-13448, Tvu-16073, and 2305675 were identified to be tightly clustered sharing several common alleles with the drought tolerant variety Danila when analyzed with SSR and SNP markers. The findings will assist and contribute to future genetic diversity studies aimed at the genetic improvement of local Eastern Africa cowpea accessions for improved overall agronomic performance in general and breeding for drought tolerant in particular.  相似文献   
3.
The objective of this study was to assess the effect of environmental variations on the abundance of Sardinella aurita and Sardinella maderensis in Senegalese waters in the upwelling system. Monthly data indicating the abundance of sardinella were first estimated from commercial statistics, using Generalized Linear Model from 1966 to 2011. Abundance indices (AIs) were then compared with environmental indices, at the local scale, a Coastal Upwelling Index (CUI) and a coastal Sea Surface Temperature (SST) index, and on a large scale, the North Atlantic Oscillation (NAO), the Atlantic Multidecadal Oscillation (AMO) and the Multivariate El Niño Southern Oscillation Index (MEI), using correlations and times series analyses. The results showed that the abundance of sardinella is determined by a strong seasonal pattern and inter‐annual fluctuations. The abundance of S. aurita peaked in spring and in autumn, whereas that of S. maderensis peaked in the warm season (July–September). The trend of the sardinella abundance was significantly correlated with the CUI, especially in autumn and spring. Interannual fluctuations of S. maderensis and S. aurita abundance are, respectively, driven by the precocity and the duration of the upwelling season that is attributed to distinct migration patterns. Both sardinella species also respond with a delay of around 4 years to the winter NAO index and the autumn CUI, and the AMO index, respectively, both related to migration patterns. The wide variations in sardinella biomass are caused by variations in environmental conditions, which should be considered in the implementation of an ecosystem‐based approach in sardinella stocks management.  相似文献   
4.
We investigate Sardinella aurita and Sardinella maderensis recruitment success relative to the variability of oceanographic conditions in Senegalese waters using generalized additive models (GAM). Results show that recruitment of both species is marked by a strong intra‐annual (seasonal) variation with minimum and maximum in winter and summer, respectively. Their interannual variations are synchronous until 2006 (recruitment decreasing), while from 2007 there is no synchrony. The model developed shows that sardinella recruitment variability is closely related to the tested environmental variables in the study area. However, the key environmental variables influencing the recruitment success are different for both species: the Coastal Upwelling Index and the sea surface temperature for S. aurita and S. maderensis, respectively. We report that recruitment success of S. aurita and S. maderensis are associated with distinct ranges of sea surface temperature, upwelling intensity, wind‐induced turbulence, concentration of chlorophyll‐a and north Atlantic oscillation index. Considering food security and socio‐economic importance of both stocks, we recommend that consideration is given to the environmental variability in the small pelagic fish national management plans, particularly in the context of climate change.  相似文献   
5.
The parasitic weed, Striga gesnerioides, is a major threat to cowpea productivity throughout the savannas of West and Central Africa. The identification of sources of S. gesnerioides resistance and their incorporation into breeding programs would be a beneficial strategy to combat the devastation caused by the parasite in cowpea fields. In this study we examined one hundred and ninety-four (194) accessions, four commercial varieties and two controls collected from a mini core collection of cowpea held at the International Institute of Tropical Agriculture genebank for resistance to S. gesnerioides race 3 (SG3), the most prevalent race in Nigeria, using phenotypic screening and molecular marker analysis. Our studies identified two cowpea accessions, Tvu-1272 and Tvu-16514, that are resistant to S. gesnerioides SG3. Resistance in these lines is associated with the molecular marker SSR1, known to segregate with the gene conferring resistance to SG3 in the cultivar B301. Phenotypically, resistance in Tvu-1272 and Tvu-16514 is expressed as a hypersensitive response at the site of infection on the roots. Allelism tests indicated that the gene that conferring SG3 resistance in Tvu-1272 is independent of that conferring resistance in B301. Tvu-1272 and Tvu-16514 will provide additional new sources of resistance to Striga and races prevalent in Nigeria.  相似文献   
6.
Journal of Crop Science and Biotechnology - Increasing sorghum production per unit area in the farmer’s field by developing high yielding tannin-free and grain mold tolerant varieties in...  相似文献   
7.
Cowpea, Vigna unguiculata (L.), is an important grain legume grown in the tropics where it constitutes a valuable source of protein in the diets of millions of people. Some abiotic and biotic stresses adversely affect its productivity. A review of the genetics, genomics and breeding of cowpea is presented in this article. Cowpea breeding programmes have studied intensively qualitative and quantitative genetics of the crop to better enhance its improvement. A number of initiatives including Tropical Legumes projects have contributed to the development of cowpea genomic resources. Recent progress in the development of consensus genetic map containing 37,372 SNPs mapped to 3,280 bins will strengthen cowpea trait discovery pipeline. Several informative markers associated with quantitative trait loci (QTL) related to desirable attributes of cowpea were generated. Cowpea genetic improvement activities aim at the development of drought tolerant, phosphorus use efficient, bacterial blight and virus resistant lines through exploiting available genetic resources as well as deployment of modern breeding tools that will enhance genetic gain when grown by sub‐Saharan Africa farmers.  相似文献   
8.
Legumes are important components of sustainable agricultural production, food, nutrition and income systems of developing countries. In spite of their importance, legume crop production is challenged by a number of biotic (diseases and pests) and abiotic stresses (heat, frost, drought and salinity), edaphic factors (associated with soil nutrient deficits) and policy issues (where less emphasis is put on legumes compared to priority starchy staples). Significant research and development work have been done in the past decade on important grain legumes through collaborative bilateral and multilateral projects as well as the CGIAR Research Program on Grain Legumes (CRP‐GL). Through these initiatives, genomic resources and genomic tools such as draft genome sequence, resequencing data, large‐scale genomewide markers, dense genetic maps, quantitative trait loci (QTLs) and diagnostic markers have been developed for further use in multiple genetic and breeding applications. Also, these mega‐initiatives facilitated release of a number of new varieties and also dissemination of on‐the‐shelf varieties to the farmers. More efforts are needed to enhance genetic gains by reducing the time required in cultivar development through integration of genomics‐assisted breeding approaches and rapid generation advancement.  相似文献   
9.
A better understanding of protective immune memory against contagious bovine pleuropneumonia (CBPP) is needed in order to facilitate the development of safer vaccines based on selected components of the pathogen. For this purpose, cells collected from lymph nodes draining the lungs of Mycoplasma mycoides subsp. mycoides small colony biotype (MmmSC)-infected cattle were stimulated with the pathogen in vitro and evaluated concurrently for proliferation (CFSE based method), expression of activation, memory markers and cytokine production. Direct evidence is presented for a major contribution of CD4+ T cells to the vigorous proliferative and T1 biased cytokine recall responses observed in cattle that have recovered from infection but not in animals developing the acute form of the disease. Two different phenotypes of MmmSC-specific memory CD4 were observed based on CD62L expression and proliferative capacities. Furthermore, recall proliferation of B cells also occurred but was strictly dependent on the presence of CD4. The information provided in this study will facilitate the search for MmmSC antigens that have potential for the development of subunit vaccines against CBPP.  相似文献   
10.
Flower bud thrips, Megalurothrips sjostedti is the most severe field pest of cowpea that causes massive flower abortion which eventually results to substantial yield reduction in Africa. There is paucity of information on the mode of gene actions controlling inheritance of resistance to flower bud thrips in cowpea in the literature. The objectives of study were to assess the genetic variability for thrips resistance among the cowpea germplasm, determined the mode of inheritance of genes that conferred resistance and both broad and narrow-sense heritability estimates for the inheritance of thrips resistance in cowpea. Twelve cowpea lines were used in crosses in the screen house at IITA, Ibadan. The mating was accomplished using North Carolina design II to generate 48F1 hybrids, which were eventually evaluated with the parents. Data on number of peduncles, number of pods and number of thrips per flower were recorded and subjected to analysis of variance using random model by SAS 9.2. Significant variability was observed for most agronomic and thrip-adaptive traits among the cowpea germplasm, parental-lines and F1 genotypes evaluated. General combining ability (GCA) and specific combining ability (SCA) mean squares were significant (P < 0.01) for number of pods per plant and other traits under the research environment. The GCA effect accounted for 68.82–80.07% of the total variation among hybrids for all traits except days to flowering; SCA explained less than 50% of the total variation. Narrow-sense heritability estimates ranged from 7.53 (days to flower) to 63.92% (number of peduncles per plant). Additive gene action largely controlled the inheritance of yield components and other traits under thrips infestation and these traits were moderately heritable.  相似文献   
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