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Full potential of any hybrid can be exploited by ensuring the supply of genetically pure seeds. Conventionally, hybrid seed
purity assessment is done through grow out test (GOT) which is based on the morphological and floral characters of plants
grown to maturity. Being land and labor intensive, time consuming and influenced by the environment, there is an immense need
to replace GOT with a simple, rapid, unbiased and cost-effective DNA based assay for hybrid purity assessment. With this objective,
the parental lines of three commercial safflower hybrids of India (NH-1, NH-15 and DSH-129) were screened using 74 safflower
EST-SSR markers and five markers were found to be polymorphic. A PCR-based assay with these markers showed both alleles of
the parental lines in pure hybrids proving the heterozygosity, while the off-types were identified by the presence of either
of the parental alleles. This assay could accurately determine the genetic purity in a predetermined sample of hybrids constituted
by deliberately mixing seeds of parental lines. This is the first report demonstrating the utility of EST-SSR markers for
the assessment of genetic purity of hybrids in crop plants. 相似文献
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Passoupathy Rajendrakumar Akshaya Kumar Biswal Kannabiran Sakthivel Maganti Sheshu Madhav Chirravuri Neeraja Sena M. Balachandran Kommoju Srinivasarao Podishetty Natarajkumar Yadla Hari Kalidindi Sujatha Raman M. Sundaram 《Euphytica》2009,169(2):263-271
SSR markers targeting (GATA)
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motifs are known to be highly polymorphic and useful in many organisms. (GATA)
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motif specific SSR markers covering the whole rice genome are not available. The present study was carried out with an objective
to identify class I rice microsatellites in the rice genome with (GATA)n motifs, in-silico, and validate their potential as molecular markers. A total of 243 such motifs were identified; 65 of these
were present in the genic region, 59 were in the upstream region and the remaining motifs were found in the intergenic regions.
Many of the (GATA)
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motifs were found within and/or upstream of genes associated with biotic or abiotic stress tolerance. A total of 230 PCR-based
markers targeting all the class I (GATA)
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microsatellites were developed and 35 of these markers spread across the rice genome were validated in a set of 24 representative
rice varieties belonging to five distinct cultivar groups. All the markers were polymorphic, with average polymorphism information
content (PIC) value of 0.61, and the rice cultivars could be uniquely distinguished into different cultivar groups based on
marker analysis. These informative markers targeting (GATA)
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motifs representing a new set of markers in rice will be highly useful for genetic studies and marker-assisted selection.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users.
Passoupathy Rajendrakumar, Akshaya Kumar Biswal and Kannabiran Sakthivel contributed equally. 相似文献
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I. Jaikishan P. Rajendrakumar M. S. Ramesha B. C. Viraktamath S. M. Balachandran C. N. Neeraja K. Sujatha K. Srinivasa Rao P. Natarajkumar Y. Hari K. Sakthivel A. S. Ramaprasad R. M. Sundaram 《Plant Breeding》2010,129(1):108-111
This study was undertaken to assess the comparative potential of 25 Expressed Sequence Tag derived simple sequence repeats (EST-SSRs) and 25 genomic SSRs in the prediction of grain yield heterosis using a set of nine cytoplasmic male sterile (CMS) lines and 32 restorer lines of rice. EST-SSRs and genomic SSRs exhibited an average Polymorphism Information Content value of 0.37 and 0.45, respectively. The coefficient of marker polymorphism among parental lines with respect to a set of hypervariable EST and genomic SSRs was correlated with standard heterosis for grain yield of six public bred rice hybrids. EST-SSRs gave a better correlation (r = 0.75) as compared with genomic SSRs (r = 0.09). When 10 'key' informative EST-SSR markers which showed a higher positive correlation with grain yield heterosis were validated in a new set of 14 experimental hybrids, the markers exhibited a higher correlation (r = 0.79), indicating the predictive value of these EST-SSRs. We recommend these 10 'key' informative EST-SSR markers for analysis of genetic diversity of parental lines and prediction of heterosis in hybrid rice breeding programmes. 相似文献
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