全文获取类型
收费全文 | 148篇 |
免费 | 8篇 |
专业分类
林业 | 8篇 |
农学 | 50篇 |
基础科学 | 5篇 |
28篇 | |
综合类 | 5篇 |
农作物 | 9篇 |
水产渔业 | 11篇 |
畜牧兽医 | 35篇 |
园艺 | 2篇 |
植物保护 | 3篇 |
出版年
2022年 | 3篇 |
2021年 | 2篇 |
2020年 | 4篇 |
2019年 | 14篇 |
2018年 | 8篇 |
2017年 | 2篇 |
2016年 | 13篇 |
2015年 | 12篇 |
2014年 | 6篇 |
2013年 | 10篇 |
2012年 | 7篇 |
2011年 | 12篇 |
2010年 | 12篇 |
2009年 | 4篇 |
2008年 | 7篇 |
2007年 | 8篇 |
2006年 | 8篇 |
2005年 | 2篇 |
2004年 | 7篇 |
2003年 | 5篇 |
2001年 | 2篇 |
2000年 | 1篇 |
1999年 | 1篇 |
1998年 | 1篇 |
1995年 | 1篇 |
1993年 | 1篇 |
1987年 | 1篇 |
1980年 | 1篇 |
1979年 | 1篇 |
排序方式: 共有156条查询结果,搜索用时 16 毫秒
1.
Sameer Kumar Chanda Venkata Ganga Rao Nadigatla Veera Prabha Rama Rachit K. Saxena Kulbhushan Saxena Hari D. Upadhyaya Moses Siambi Said N. Silim Kothapally Narasimha Reddy Anupama J. Hingane Mamta Sharma Shivali Sharma Stephen Dominic Lyimo Rose Ubwe Meshack Makenge Kananji Gad Paul Kiprotich Kimurto Manuel Amane Kennedy Kanenga Yuventino Obong Emanuel Monyo Chris Ojiewo Nagesh Kumar Mallela Venkata Jaganmohan Polineni Rao Prashanthi Lakkireddy Sudhakar Chourat Indraprakash Singh Sobhan Sajja Shruthi Hirikara Beliappa Rajeev K. Varshney 《Plant Breeding》2019,138(4):445-454
In the past five decades, constant research has been directed towards yield improvement in pigeonpea resulting in the deployment of several commercially acceptable cultivars in India. Though, the genesis of hybrid technology, the biggest breakthrough, enigma of stagnant productivity still remains unsolved. To sort this productivity disparity, genomic research along with conventional breeding was successfully initiated at ICRISAT. It endowed ample genomic resource providing insight in the pigeonpea genome combating production constraints in a precise and speedy manner. The availability of the draft genome sequence with a large‐scale marker resource, oriented the research towards trait mapping for flowering time, determinacy, fertility restoration, yield attributing traits and photo‐insensitivity. Defined core and mini‐core collection, still eased the pigeonpea breeding being accessible for existing genetic diversity and developing stress resistance. Modern genomic tools like next‐generation sequencing, genome‐wide selection helping in the appraisal of selection efficiency is leading towards next‐generation breeding, an awaited milestone in pigeonpea genetic enhancement. This paper emphasizes the ongoing genetic improvement in pigeonpea with an amalgam of conventional breeding as well as genomic research. 相似文献
2.
伤口愈合是一种复杂的现象,是机体控制的系统对细胞进行精微同步调节的结果.然而,一系列可变因素通过多种方法来影响此过程.通常来说,机体内的各种维生素,矿物质,脂肪酸,氨基酸和其他营养物质对该过程起着直接或间接的重要影响.不仅如此,各种疾病与无数的外界因素也以不可预料的形式影响着该过程.因此,任何治疗方法都是努力控制上述因素以使机体处于最佳恢复状态.因此,当这种天然愈合过程被打断,机体的伤口就会转变为慢性,或者出现溃疡. 相似文献
3.
4.
Brajesh C. Varshney N.M. Ponnanna Pranati A. Sarkar Pragna Rehman Jigar H. Shah 《Journal of veterinary science (Suw?n-si, Korea)》2007,8(1):57-64
Escherichia coli (E. coli) strains were collected from young diarrheic calves in farms and field. Strains that expressed the K99 (F5) antigen were identified by agglutination tests using reference antibodies to K99 antigen and electron microscopy. The K99 antigen from a selected field strain (SAR-14) was heat-extracted and fractionated on a Sepharose CL-4B column. Further purification was carried out by sodium deoxycholate treatment and/or ion-exchange chromatography. Monoclonal antibodies to purified K99 antigen were produced by the hybridoma technique, and a specific clone, NEK99-5.6.12, was selected for propagation in tissue culture. The antibodies, thus obtained, were affinity-purified, characterized and coated onto Giemsa-stained Cowan-I strain of Staphylococcus aureus (S. aureus). The antibody-coated S. aureus were used in a co-agglutination test to detect K99+ E. coli isolated from feces of diarrheic calves. The specificity of the test was validated against reference monoclonal antibodies used in co-agglutination tests, as well as in ELISA. Specificity of the monoclonal antibodies was also tested against various Gram negative bacteria. The developed antibodies specifically detected purified K99 antigen in immunoblots, as well as K99+ E. coli in ELISA and co-agglutination tests. The co-agglutination test was specific and convenient for large-scale screening of K99+ E. coli isolates. 相似文献
5.
6.
Protection of Procambarus clarkii against white spot syndrome virus using recombinant subunit injection vaccine expressed in Pichia pastoris 总被引:1,自引:0,他引:1
ABSTRACT: The potentiality of injection vaccine against white spot syndrome virus (WSSV) in crayfish Procambarus clarkii was investigated. WSSV envelope proteins VP19 and VP28 were expressed in yeast Pichia pastoris GS115. The purified recombinant proteins (2 µg/g of crayfish) were injected intramuscularly, and the same dose injected as a booster shot on fifth day after vaccination. The vaccinated crayfish were divided into two even groups and later challenged orally by WSSV-infected dead crayfish muscle (2 g/individual) on the third and 21st days after the booster shot. The relative percent survival (RPS) in the third-day group was the highest in VP28 (91%), followed by VP19 + VP28 (84%), and VP19 (45%). The RPS for the 21st-day group was the highest in VP28 (78%), followed by VP19 + VP28 (76%), and VP19 (17%). Development of vaccine by using recombinant proteins VP19 and VP28 expressed in yeast is feasible. 相似文献
7.
Kumar Chavlesh Singh Sanjay Kumar Verma Mahendra Kumar Pramanick Kallol Kumar Srivastav Manish Kaur Sukhdeep Kumar Rajeev Ranjan 《Genetic Resources and Crop Evolution》2022,69(2):645-659
Genetic Resources and Crop Evolution - The wild Malus germplasm is considered as a gene reservoir for various biotic and abiotic stresses tolerance/resistance genes, including important novel... 相似文献
8.
9.
10.
Molecular mapping of a locus controlling resistance to Albugo candida in Indian mustard 总被引:1,自引:0,他引:1
Brassica juncea (L.) Czern & Coss is widely grown as an oilseed crop in the Indian subcontinent. White rust disease caused by Albugo candida (Pers.) Kuntze is a serious disease of this crop causing considerable yield loss every year. The present study was undertaken to identify molecular markers for the locus controlling white rust resistance in a mustard accession, BEC‐144, using a set of 94 recombinant inbred lines (RILs). The screening of individual RILs using an isolate highly virulent on the popular Indian cultivar ‘Varuna’ revealed the presence of a major locus for rust resistance in BEC‐144. Based on screening of 186 decamer primers employing bulked segregant analysis (BSA), 11 random amplified polymorphic DNA markers were identified, which distinguished the parental lines and the bulks. Five of these markers showed linkage with the rust resistance locus. Two markers, OPN0l000 and OPB061000, were linked in coupling and repulsion phases at 9.9 cM and 5.5 cM, respectively, on either side of the locus. The presence of only two double recombinants in a population of 94 RILs suggested that the simultaneous use of both markers would ensure efficient transfer of the target gene in mustard breeding programmes. 相似文献